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Citations for Thermo Fisher :
6701 - 6750 of 10000+ citations for 1 2 Dihydro 1 2 tetrahydro 2H pyran 2 yl oxy ethyl 5H tetrazole 5 thione d4 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: SARS-CoV-1 (Genbank AAP13567) or SARS-CoV-2 (Genbank MN908947) S proteins were transiently expressed in Freestyle 293F system (ThermoFisher) whereas RBD proteins were expressed in the Expi293 system (ThermoFisher) ...
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bioRxiv - Immunology 2022Quote: ... washed with complete DMEM (DMEM + 2 mM L-glutamine, 1 mM sodium pyruvate, 10 mM HEPES, 200 U/mL penicillin/streptomycin, 10% FBS; GIBCO) and resuspended in complete DMEM in 15 cm tissue culture plates overnight ...
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bioRxiv - Immunology 2021Quote: ... Some of the precipitates were eluted in the mixture of IP Buffer and SDS sample buffer (2:1) to elute proteins and the others were eluted in TRIzol Reagent (Invitrogen) for RNA isolation.
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bioRxiv - Biochemistry 2020Quote: ... one µg of small RNAs was mixed with 2 µg of HPDP-Biotin (ThermoFisher Scientific, 1 mg/ml in DMSO). The reaction was incubated for three hours at room temperature protected from light and mixed every 15 minutes ...
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bioRxiv - Cancer Biology 2020Quote: ... cell line GRANTA-519 waskept in culture in RPMI 1640 medium supplemented with 10% heat-inactivated FBS 2 mM L-glutamine and 1% penicillin/streptomycin antibiotics (Gibco® ...
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Therapy-induced lipid uptake and remodeling underpin ferroptosis hypersensitivity in prostate cancerbioRxiv - Cancer Biology 2020Quote: ... DNA and F-actin were counterstained for 20 min in the dark with 1 μL/ml 4’,6-diamidino-2-phenylindole (DAPI, Invitrogen) and Alexa Fluor 647 phalloidin (Thermo Fisher) ...
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bioRxiv - Genetics 2020Quote: ... and exposed to 1% 2-hydroxy-propyl-beta-cyclodextrin for 45min were labelled with 50 μg/ml DiI-LDL (Invitrogen) for 30 min at 4 °C ...
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bioRxiv - Microbiology 2021Quote: ... The 5’ and 3’ fragments (2 μg of each) were ligated for 1 h at 16°C by T4 DNA ligase (Invitrogen) and purified by phenol-chloroform extraction ...
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bioRxiv - Biophysics 2021Quote: ... the grids were blotted for 2 s with a blot force of 1 in a Vitrobot Mark IV (Thermo Fisher) and plunge-frozen in liquid ethane ...
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bioRxiv - Immunology 2021Quote: Released tissue cells were stained in aliquots of 1 ×106 cells/100 μL of PBS with 2% fetal calf serum (FCS, GIBCO) and 0.1% NaN3 for 30min on ice ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... Sections were then incubated for 2 h at room temperature with anti-mouse Alexa 488 (Invitrogen A-1101, 1:500) and anti-rabbit Cy3 (Invitrogen A-10520 ...
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bioRxiv - Immunology 2021Quote: ... then the lysate equivalent to 2×105 cells was mixed at a 1:4 ratio with 4x LDS buffer containing 10% sample reducing agent (Invitrogen). Samples were heated at 95°C for 5 minutes and collected by centrifugation before loading.
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bioRxiv - Neuroscience 2020Quote: ... the free-floating sections were incubated for 2 h in staining solution together with anti-rat Alexa 594 and anti-rabbit Alexa 647 (1:400, ThermoFisher). Slices were washed 3 times with PBS and mounted using Dako Fluorescent mounting medium (Agilent) ...
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bioRxiv - Developmental Biology 2021Quote: ... Nuclei-beads were eluted in 1 ml of NPBt as described above and the NPBt removed using a magnetic stand (DynaMag TM-2 magnet, Invitrogen). Nuclei-beads were then processed for RNA extraction.
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bioRxiv - Neuroscience 2020Quote: ... and AlexaFluor 555-conjugated F(ab’)2 fragment goat anti-rabbit (1:500; A21428, Invitrogen, Thermo Fisher Scientific, Waltham, MA). After five 5-minute washes with PBS ...
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bioRxiv - Neuroscience 2020Quote: ... AlexaFluor 647-conjugated F(ab’)2 fragment donkey anti-mouse (1:500; A31571, Life Technologies, Thermo Fisher Scientific, Waltham, MA), and AlexaFluor 555-conjugated F(ab’)2 fragment goat anti-rabbit (1:500 ...
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bioRxiv - Neuroscience 2020Quote: ... and AlexaFluor 555-conjugated F(ab’)2 fragment goat anti-rabbit (1:500; A21428, Invitrogen, Thermo Fisher Scientific, Waltham, MA). After five 5-minute washes with PBS ...
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bioRxiv - Neuroscience 2020Quote: ... AlexaFluor 647-conjugated F(ab’)2 fragment donkey anti-mouse (1:500; A31571, Life Technologies, Thermo Fisher Scientific, Waltham, MA), and AlexaFluor 555-conjugated F(ab’)2 fragment goat anti-rabbit (1:500 ...
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bioRxiv - Cancer Biology 2021Quote: ... The Click-iT reaction was performed for 30 min at room temperature by suspending the cells in Click-iT cell reaction buffer containing 1 mM azide-modified Alexa 488 and 2 mM CuSO4 (Invitrogen). Cells were washed twice in PBS with 1% FCS ...
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bioRxiv - Biochemistry 2020Quote: ... The aqueous supernatant was transferred to a new 2 ml screw-top tube to which 400 μl of 24:24:1 phenol:chloroform:isoamyl alcohol (Ambion cat. # 9732) was added ...
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bioRxiv - Bioengineering 2021Quote: ... the cells were incubated with 2 μM EthD-1 and 4 μM Calcein AM solutions of cell viability/cytotoxicity kit (Invitrogen) for 20 minutes ...
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bioRxiv - Biophysics 2021Quote: ... slides were dehydrated through an ethanol series and incubated with 2 × SSC supplemented with 100 µg ml−1 RNase A (Invitrogen) at 37°C for 60 min ...
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bioRxiv - Neuroscience 2021Quote: ... the sections were incubated with goat anti-rabbit IgG Alexa 488 secondary antibody (1:500 for 2 h at 4°C; Invitrogen) in solution with PBS ...
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bioRxiv - Neuroscience 2020Quote: ... Validation of shRNA constructs for reduction of Arl8b was done by transfecting mouse N2a cells at 40% confluency with pLKO3.G-Arl8b shRNA #1 and #2 either separately or together using Lipofectamine 2000 (Invitrogen). Transfection rates were > 80% and were ascertained by counts of GFP fluorescence on cell bodies ...
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bioRxiv - Cancer Biology 2020Quote: All 2-D monolayer cultures (2-D) were maintained in standard RPMI media supplemented with 10% fetal bovine serum (FBS) and 1% penicillin-streptomycin (Gibco) in tissue-culture treated flasks at 37°C with 5% CO2 ...
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bioRxiv - Cell Biology 2021Quote: ... the cells were washed three times with PBS and nuclei counterstained with 1 µg/ml 4’,6-diamidino-2-phenylindole (DAPI) (#D3571, Invitrogen) in PBS for 30 min at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... on 12 or 15 mm coverslips in a 6-well plate and 10 nM siRNA-1 or -2 was transfected using RNAi MAX reagents (Invitrogen) according the manufacturer protocol ...
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bioRxiv - Cell Biology 2021Quote: T47D cells were seeded in six-well plates (50 000 cells/well) in 2 ml of cell medium (10% hiFBS [Fisher] and 1% Pen/Strep [Invitrogen]). On Day 1 ...
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bioRxiv - Neuroscience 2020Quote: ... 1-2 µg total RNA were reverse transcribed into cDNA using the Superscript III enzyme and oligo(dT) primers (Invitrogen). qPCR was performed using SYBR Green (Quanta) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Copies of SARS-CoV-2 E gene were measured by qRT-PCR TaqMan Fast Virus 1-step assay (Thermo Fisher). SARS-CoV-2 specific primers and probes from the 2019-nCoV RUO Assay kit (Integrated DNA Technologies ...
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bioRxiv - Biochemistry 2021Quote: B16-F1 cells and derived CapZa1/2 CRISPR#1 and CapZb KO#10 were cultured in DMEM (4.5 g/L glucose; Invitrogen, Germany), supplemented with 10% FCS (Gibco) ...
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bioRxiv - Systems Biology 2021Quote: ... a portion of each kidney was added to 1 mL of Nuclei EZ lysis buffer supplemented with 2% protease inhibitor (Thermofisher), and 1% Superase (Thermofisher) ...
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bioRxiv - Microbiology 2020Quote: ... cells were fixed with 2% PFA at room temperature for 20 minutes and were incubated in 1:10,000 Hoescht 33342 (Life Technologies) in PBS at 4°C overnight ...
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bioRxiv - Neuroscience 2020Quote: ... sections were incubated for 2 hours at RT with secondary antibodies: goat anti-rabbit Alexa Fluor633 (1:500, Invitrogen, A21070), goat anti-mouse Alexa Fluor488 (Jackson Immunoresearch 115-545-003 ...
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bioRxiv - Microbiology 2021Quote: ... PMA differentiated THP-1 cells were treated with 10 μg/mL mouse monoclonal anti-human IFNAR2 (ThermoFisher Scientific, MMHAR-2), or human IgG (Invitrogen ...
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bioRxiv - Microbiology 2020Quote: ... wells were stained with FM 1-43 FX membrane dye (100 µl per channel, 2-10 µg/ml, F35355, Thermofisher) in PBS for 30 min on ice ...
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bioRxiv - Molecular Biology 2021Quote: ... Nuclease S1 cleavage was carried out for 15 minutes at room temperature in 1× Reaction Buffer (40 mM sodium acetate pH 4.5, 300 mM NaCl and 2 mM ZnSO4; Thermo Scientific), and 0.1 μg of total yeast tRNA (Ambion ...
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bioRxiv - Microbiology 2020Quote: ... Cells are washed 5 times with 200 μL of PBS and incubated with 100 μL of the secondary antibody anti-rabbit (1/1000 in 2% BSA in PBS, Goat anti-Rabbit Alexa Fluor 647 #A21245 – Invitrogen) for 45 min at 4°C in a humidity chamber ...
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bioRxiv - Cancer Biology 2020Quote: ... Harvested cells were spinned at 400g for 5 min and pellet was suspended at 1×106 cells per ml in pre-warmed DMEM (supplemented with 2% serum) media (Gibco, Invitrogen) in two tubes ...
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bioRxiv - Neuroscience 2020Quote: ... the sections were incubated for 2 hours at room temperature with an anti-DNP antibody conjugated with Alexa 488 (1/500, Invitrogen). After quenching HRP activity and washing ...
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bioRxiv - Cell Biology 2021Quote: iPSC-CMs cultured in chamber slides were loaded with 1 μM Rhod-2 (Molecular Probes, Life Technologies, Carlsbad, CA, USA) in Hank’s balanced salts modified buffer (HBSS ...
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bioRxiv - Cell Biology 2021Quote: iPSC-CMs cultured in chamber slides were loaded with 1 μM Rhod-2 (Molecular Probes, Life Technologies, Carlsbad, CA, USA) in Hank’s balanced salts modified buffer (HBSS ...
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bioRxiv - Biophysics 2020Quote: ... incubated 1 hour with Alexa Fluor 647-conjugated cross-adsorbed goat anti-mouse IgG F(ab’)2 (A-21237, Invitrogen) diluted at 1:1000 in 3% BSA/PBS ...
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bioRxiv - Cancer Biology 2021Quote: ... Coverslips were rinsed for 2 min in Click-iT reaction rinse buffer and stained in Hoechst solution (1:10,000, Invitrogen H3570) for 5 min at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... Secondary antibodies were applied in incubation buffer (1:500 in PBS/BSA) with 4‘,6-diamidino-2-phenylindole (DAPI, ThermoFisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... washed twice in FACS buffer (1X PBS with 2% FBS and 1 mM EDTA) and the data were acquired using the Attune NxT flow cytometer (ThermoFisher). For FL phenotyping ...
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bioRxiv - Cancer Biology 2022Quote: ... 1 mM DTT) at 37°C for 2 h followed by incubation with 0.75 U UDG (EN0361, Thermo Fisher Scientific) in 1x UDG buffer at 37°C for 45 min ...
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bioRxiv - Immunology 2022Quote: ... The appropriate volume of cells was then pelleted at 1500rpm for 5 minutes after which the supernatant was decanted and the cells were resuspended at a 2:1 ratio of RPMI media (Gibco) + FLIPR Calcium 6 dye (Molecular Devices) ...
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bioRxiv - Immunology 2022Quote: ... Antigens were separately diluted down to a concentration of 2uM in 50uL of the 2:1 ratio of RPMI media (Gibco) + FLIPR Calcium 6 dye (Molecular Devices ...
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bioRxiv - Immunology 2022Quote: ... and reduced at 37°C with 1:125 dilution of Tris (2-carboxyethyl) phosphine hydrochloride (TCEP) (ThermoFisher, Waltham, MA, USA) for 30 minutes ...