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Citations for Thermo Fisher :
701 - 750 of 10000+ citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... or AlexaFluor647 (Thermo Fisher) for confocal ...
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bioRxiv - Cancer Biology 2024Quote: ... Cell viability was determined using the Zombie Aqua Fixable Viability Kit (Invitrogen). Samples were acquired using BD FACS Calibur ...
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bioRxiv - Cancer Biology 2024Quote: ... HEK293-FT (RRID: CVCL_6911) was purchased from Thermo Fisher Scientific (#R70007) ...
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bioRxiv - Cancer Biology 2024Quote: ... fixed cells were incubated after the first set of washes with streptavidin conjugated to AlexaFluor647 (Thermo Fisher) diluted in high-salt buffer (0.5 M NaCl ...
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bioRxiv - Cancer Biology 2024Quote: ... placed onto Superfrost PlusTM slides (Fisher Scientific) and fixed in -20°C acetone for 5 minutes before air drying ...
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bioRxiv - Cancer Biology 2024Quote: ... SuperSignal™ West Pico PLUS Chemiluminescent Substrate or SuperSignal™ West Femto Maximum Sensitivity Substrate (Thermo Fisher) were used to detect HRP-conjugated secondary antibodies according to manufacturer’s instructions prior to imaging ...
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bioRxiv - Cancer Biology 2024Quote: ... For the minigene assays 500 ng RNA was reverse transcribed using SuperScriptTM IV Reverse Transcriptase (Thermo Fisher Scientific, #18090010) with the plasmid specific BGH-rev oligo in 20 µl ...
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bioRxiv - Cancer Biology 2024Quote: ... Membranes were labeled using AlexaFluor488- or AlexaFluor647-conjugated wheat germ agglutinin (WGA; Thermo Fisher). Slides were washed 6 times as before and mounted using Prolong Gold Antifade (Thermo Fisher ...
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bioRxiv - Cancer Biology 2024Quote: RNA was extracted using the PureLinkTM RNA mini extraction kit (Thermo Fisher) and on-column DNA digestion was completed using PureLinkTM DNase according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... Real-time PCR was performed using the SYBR select Master Mix for CFX (Thermo Fisher) in hard-shell 96-well PCR plates (Bio-Rad Laboratories ...
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bioRxiv - Cancer Biology 2024Quote: Genomic DNA from mouse leukemia samples was isolated using the GeneJET Genomic DNA purification kit (Thermo Fisher Scientific), following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... BTSCs were dissociated into single cell suspension and 3 x 104 cells were plated on 48- well plates coated with poly-D-Lysine (Thermofisher, #A38904-01). When cells adhered to the plate ...
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bioRxiv - Cancer Biology 2024Quote: ... 100 U/ml penicillin-streptomycin (Fisher Scientific, #11548876), 2 mM GlutaMAX (ThermoFischer ...
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bioRxiv - Cancer Biology 2024Quote: ... permeabilized and stained using the Click-iT EdU cell proliferation kit (Thermo Fisher Scientific, #C10340) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... followed by DNase digestion using the DNase I Amplification Grade Kit (Invitrogen) and a clean-up using RNeasy Min Elute columns (Qiagen) ...
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bioRxiv - Cancer Biology 2024Quote: ... Reverse transcription was performed using the TaqMan Reverse Transcription kit (ThermoFisher Scientific, N8080234). cDNA was detected using PowerUp SYBR Green Master Mix for qPCR (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were transfected with Lipofectamine RNAiMAX transfection reagent (ThermoFisher Scientific, 13778150) as follows ...
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bioRxiv - Cancer Biology 2024Quote: ... in 2mL of Neurobasal media (Fisher Scientific, #11570556) supplemented with 1X B-27 supplement (ThermoFischer ...
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bioRxiv - Cancer Biology 2024Quote: BTSCs were dissociated to single cell suspension using Accutase solution (Fisher Scientific, #15323609), plated in low-attachment plates or flasks ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were washed three times with 1X PBS and then incubated with secondary antibody for 30 minutes rocking at room temperature (Goat anti-Rabbit IgG Alexa Fluor 488; ThermoFisher Scientific, A-11008). Cells were then washed two times with 1X PBS and imaged on the Keyence BZ-X800 microscope.
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bioRxiv - Cancer Biology 2024Quote: Total proteins were harvested in RIPA lysis buffer containing protease and phosphatase inhibitors (Thermo Fisher Scientific, #A32959). Protein concentration was determined by Bradford assay (Sigma ...
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bioRxiv - Cancer Biology 2024Quote: ... and 100 U/ml penicillin-streptomycin (Fisher Scientific, #11548876).
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bioRxiv - Cancer Biology 2024Quote: ... GlutaMAX supplement (ThermoFisher, 35050061) was added to a final concentration of 1X ...
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bioRxiv - Cancer Biology 2024Quote: ... and Vinculin (1:5000, Invitrogen, #MA511690).
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bioRxiv - Cancer Biology 2024Quote: ... the number of live cells was estimated using Calcein- AM (ThermoFisher Scientific, #C1430) / DAPI double staining ...
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bioRxiv - Cancer Biology 2024Quote: ... The cells were washed with 1X PBS and stained with FxCycle PI/RNase staining solution (Thermo Fisher Scientific, #F10797)78 ...
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bioRxiv - Cancer Biology 2024Quote: ... The ReNcell CX cells were cultured on matrigel-coated plates or flasks and maintained in DMEM/F12 media (Fisher Scientific, #11530566) supplemented with 2 mM GlutaMAX (ThermoFischer ...
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bioRxiv - Cancer Biology 2024Quote: ... neurons were plated at a density of 0.8x106 cells per well of 6-well plates coated with 50 ug/mL Poly-D-Lysine (Thermofisher, #A38904-01) and 1ug/mL Laminin (Sigma ...
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bioRxiv - Cancer Biology 2024Quote: ... recombinant (Thermo Fisher Scientific, #10342020), and specific oligonucleotides (Supplementary Table S4 ...
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bioRxiv - Cancer Biology 2024Quote: ... Slides were then washed and incubated with fluorescence-conjugated secondary antibodies (Invitrogen) at RT for 1 h ...
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bioRxiv - Cancer Biology 2024Quote: ... mounted using ProLong Diamond Antifade Mountant (Fisher Scientific, #15205739), and imaged using a slide scanner (Hamamatsu Nanozoomer 2.0HT) ...
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bioRxiv - Cancer Biology 2024Quote: ... BTSCs were recovered from cryopreservation in 10% DMSO and cultured in low attachment flasks as spheres in DMEM/F12 media (Fisher Scientific, #11530566) supplemented with 2 mM GlutaMAX (ThermoFischer ...
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bioRxiv - Cancer Biology 2024Quote: ... All libraries have been quantified using a Qubit HS dsDNA kit (Thermofisher) and the library quality check has been performed using a High Sensitivity NGS Fragment Analysis Kit on a Fragment Analyzer System (Agilent Technologies) ...
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bioRxiv - Cancer Biology 2024Quote: ... 293T cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) with 4 mM glucose (high glucose) (SH30243.01; Hyclone) and passaged with 0.25% Trypsin/EDTA (Gibco). All cell cultures were supplemented with 10% Fetal Bovine Serum (Sigma) ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were then stained in 1.5mL microcentrifuge tubes with lipophilic dyes—Vybrant CM-DiI (4 μl/mL in DPBS 1X) or Deep Red Cell Tracker (1 μl/mL in DPBS 1X, 10 mM stock) (Life Technologies), for 15 min at 37 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... All HepG2 and HUH7 cells were grown in Dulbecco’s modified Eagle’s medium (DMEM, ThermoFisher Scientific), while SNU475 cells were grown in RPMI-1640 (ATCC) ...
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bioRxiv - Cell Biology 2024Quote: N-glycans were purified using HyperSep Hypercarb SPE 10 mg cartridges (Thermo Scientific). The samples were applied to the cartridges ...
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bioRxiv - Developmental Biology 2024Quote: Purified RNA was quantified using a NanoDrop 1000 spectrophotometer (Thermo Fisher Scientific). RNA (2 μg ...
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“Identification of microRNAs regulated by E2F transcription factors in human pluripotent stem cells”bioRxiv - Developmental Biology 2024Quote: Total RNA was extracted using TRIzol reagent (Thermo Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... goat anti-guinea pig-Alexa647 (IF, Life Technologies, 1:750), goat anti-mouse (GAM)-Alexa594 (IF ...
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bioRxiv - Cell Biology 2024Quote: ... goat anti-mouse (GAM)-Alexa594 (IF, Invitrogen, 1:500), goat anti-rabbit-RRX and GAM-FITC (Jackson Laboratory ...
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bioRxiv - Cell Biology 2024Quote: ... rabbit anti-GFP (IF, Invitrogen, 1:2000), chicken anti-GFP (IF ...
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bioRxiv - Cell Biology 2024Quote: ... Nissl stain (NeuroTrace 640/660, Invitrogen, 1:2000) was used and added to secondary antibody solutions to label and identity neurons in some IF experiments in brain slices.
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bioRxiv - Cell Biology 2024Quote: N-glycan samples were loaded on a porous graphitic carbon column (100 x 0.32 mm, Thermo Scientific) using 10 mM ammonium bicarbonate buffer as the aqueous solvent (solvent A ...
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bioRxiv - Cell Biology 2024Quote: ... using Lipofectamine 3000 (Invitrogen). After 48 hr ...
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bioRxiv - Cell Biology 2024Quote: ... was developed over 17 min at a flow rate of 6 μl/min using an Ultimate 3000 capillary flow LC system (Thermo Scientific). Detection was performed with an ion trap mass spectrometer (Bruker amazon speed ETD ...
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bioRxiv - Cell Biology 2024Quote: ... washed again and mounted in ProLong Diamond (Invitrogen). For labeling with rabbit anti-GluA1 ...
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bioRxiv - Cell Biology 2024Quote: ... cells were transfected with 1μg each/well of HA-NHERF1 and/or FLAG-RGS14 (WT, mutants as indicated) using Lipofectamine 3000 (Invitrogen). 48 hr post-transfection ...
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bioRxiv - Cell Biology 2024Quote: ... Slices were then incubated in PBS supplemented with 3% normal goat serum (NGS, Invitrogen) and 0.2% Triton overnight at 4°C and then in primary antibodies diluted in the same buffer at 4°C till the next day ...
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bioRxiv - Cancer Biology 2024Quote: ... and transferred onto nitrocellulose or PVDF membranes (Li-Cor Biosciences) with the XCell transfer system (Invitrogen). Membranes were incubated at 4 °C overnight with primary antibodies and then for 1 hr with secondary donkey antibodies coupled to IRDye 680 or 800 (Li-Cor) ...