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Citations for Thermo Fisher :
801 - 850 of 10000+ citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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“Identification of microRNAs regulated by E2F transcription factors in human pluripotent stem cells”bioRxiv - Developmental Biology 2024Quote: ... then incubated for 30 minutes at 37°C with RNAse A (100 ⎧g/ml) (Invitrogen), and then with Propidium Iodide (PI ...
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“Identification of microRNAs regulated by E2F transcription factors in human pluripotent stem cells”bioRxiv - Developmental Biology 2024Quote: The RNA was extracted with mirVanaTM miRNA Isolation Kit (Ambion, Thermo Fisher Scientific) following the manufacturer’s protocols ...
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“Identification of microRNAs regulated by E2F transcription factors in human pluripotent stem cells”bioRxiv - Developmental Biology 2024Quote: ... Membranes were then incubated with the corresponding counter-antibody and the proteins were revealed by enhanced chemiluminescence detection (SuperSignal West Femto System, Thermo Scientific). For information about the antibodies used please see Supplementary Table S3 ...
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bioRxiv - Developmental Biology 2024Quote: ... The relative expression of gene was calculated as 2–ΔΔCt using the comparative Ct (ΔΔCt) method and GAPDH (Hs02758991_g1) (Thermo Fisher Scientific) as an internal housekeeping control ...
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bioRxiv - Developmental Biology 2024Quote: ... DAPI (Molecular Probes) was used 1:1000 ...
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bioRxiv - Developmental Biology 2024Quote: ... B27 w/o vitamin A (Life Technologies), N2 (Life Technologies) ...
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bioRxiv - Developmental Biology 2024Quote: ... and TaqMan gene assays (AR Hs00171172_m1; PCDH11Y Hs00263145_m1, Thermo Fisher Scientific) on a StepOnePlus System (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2024Quote: ... or Hoechst 33342 (diluted into 1 µg/ml) (ThermoFisher, Massachusetts, USA). The images were then captured using LSM 780 confocal microscope and analyzed by ZEN software (Carl-Zeiss ...
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bioRxiv - Cancer Biology 2024Quote: ... 1 µg/ml Hoechst 33342 (ThermoFisher, #62249) and 0.2% bovine serum albumin (BSA ...
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bioRxiv - Cancer Biology 2024Quote: ... 50 µg of protein lysate was prepared with 4X Bolt™ LDS Sample Buffer (ThermoFisher, #B0007) and 10X Bolt™ Sample Reducing Agent (ThermoFisher ...
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bioRxiv - Cancer Biology 2024Quote: ... and Qubit® 2.0 Fluorometer (ThermoFisher). Library preparation and sequencing was performed at the QUT Central Analytical Research Facility (CARF ...
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bioRxiv - Cancer Biology 2024Quote: ... then intracellular lipids were co-stained with 0.1 µg/mL Nile Red (Invitrogen, #N1142) and 1 µg/mL 4,6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Cancer Biology 2024Quote: ... using the Bolt Mini Blot Module (ThermoFisher, #B1000). Protein transfer using nitrocellulose membrane was conducted in 1X Bolt Transfer Buffer at 10V (ThermoFisher ...
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bioRxiv - Cancer Biology 2024Quote: ... qPCR was monitored on the ViiA-7 Real-Time PCR system (Applied Biosystems). Relative mRNA expression was then calculated using the ΔΔCT method normalising gene expression to the RPL32 house-keeping gene.
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bioRxiv - Cancer Biology 2024Quote: ... 10 mM glucose (ThermoFisher, #A2494001) and 1 mM sodium pyruvate (ThermoFisher ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were then treated with 5 µM C16:0 BODIPY (C16) (Invitrogen, #D3821), 1 µg/ml Hoechst 33342 (ThermoFisher ...
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bioRxiv - Cancer Biology 2024Quote: ... RNA concentration was quantified using the NanoDrop ND-1000 (ThermoFisher, #ND-1000) and RNA purity was subsequently assessed ...
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bioRxiv - Cancer Biology 2024Quote: ... and quantified using the NanoDrop ND-1000 spectrophotometer (ThermoFisher, #ND-1000). Viral supernatants were generated in 10 cm dishes by transfection of HEK293T cells with 0.2 µg pCMV delta R8.2 (lentiviral packaging plasmid) ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein transfer using nitrocellulose membrane was conducted in 1X Bolt Transfer Buffer at 10V (ThermoFisher, #BT0006) for 1 hour ...
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bioRxiv - Cancer Biology 2024Quote: ... and 1 µg/mL 4,6-diamidino-2-phenylindole (DAPI) (Invitrogen, #D1306) in PBS for 1 hour at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: ... Gel electrophoresis was run at 120 V (constant) for 60 minutes in 1X MOPS SDS running buffer (ThermoFisher) using the Bolt Mini Blot Module (ThermoFisher ...
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bioRxiv - Cancer Biology 2024Quote: ... siHIBCH (SASI_Hs01_00064760) or siMCCC2 (SASI_Hs01_00039625) was transfected into cells with RNAiMAX transfection reagent (ThermoFisher, #13778150) diluted 1:25 in serum free RPMI 1640 ...
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bioRxiv - Cancer Biology 2024Quote: ... and 10X Bolt™ Sample Reducing Agent (ThermoFisher, #B0009) and loaded onto a Bolt™ 4-12% Bis-Tris Mini Protein Gel (ThermoFisher ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were seeded at a density of 10,000 cells per well in standard phenol red-free RPMI 1640 (ThermoFisher, #11835055) with 5% FBS ...
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bioRxiv - Cancer Biology 2024Quote: ... and 1 mM sodium pyruvate (ThermoFisher, #11360070) adjusted to pH 7.4 ...
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bioRxiv - Cancer Biology 2024Quote: ... cDNA was then diluted (1:5) with RNAase/DNAase-free water and qRT-PCR performed using SYBR-Green Master Mix (ThermoFisher, #4309155) and primers listed in Supplementary Data ...
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bioRxiv - Cell Biology 2024Quote: ... (Invitrogen) solution was added to the pre-gel mixture (1.8 v/v%) ...
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bioRxiv - Cell Biology 2024Quote: ... anti-RAD51 (Invitrogen, MA1-23271), anti-RNF8 (14112-1-AP ...
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bioRxiv - Developmental Biology 2024Quote: ... Worms were incubated in 10µM OPP (Invitrogen) diluted with M9 buffer containing 2×108 CFU/mL of dead OP50 in a total volume of 1 mL for 3 hours at 20 °C with gentle shaking ...
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bioRxiv - Developmental Biology 2024Quote: ... The cells were selected in puromycin (Gibco; A1113803) (1.2mg/ml ...
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bioRxiv - Developmental Biology 2024Quote: ... Plasmid DNA was isolated using a midi-prep kit (Invitrogen; K210005). The integrity of the CRISPRi library was validated by Illumina Miseq through Cornell Genomics Facility (detailed library preparation steps are presented below in “Library validation and CRISPRi dropout screening”).
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bioRxiv - Developmental Biology 2024Quote: ... 1% penicillin-streptomycin (Gibco; 15140122), and 0.18% β-mercaptoethanol (Sigma ...
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bioRxiv - Cell Biology 2024Quote: ... (Thermo Fisher Scientific) for 48 h at 4 °C on a vertical rotator at 20 rpm ...
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bioRxiv - Cell Biology 2024Quote: ... Si-RNA transfections were carried out using Lipofectamine RNAiMAX (Invitrogen), according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2024Quote: ... in 50 μL Opt-MEM (Thermo Fisher Scientific). Media was changed 16 hours later to 1.5 mL fresh complete media ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were identified in Proteome Discoverer 2.4 (Thermo Fisher Scientific) using Sequest HT ...
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bioRxiv - Cell Biology 2024Quote: The sites of unsaturation in glycerophospholipids were elucidated using a high-resolution mass spectrometer (Orbitrap Elite, Thermo Scientific), modified to allow for ozone-induced dissociation (OzID ...
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bioRxiv - Cell Biology 2024Quote: ... The organic fraction was evaporated using a Savant Speedvac spd111v (Thermo Fisher Scientific) at room temperature and the remaining lipid pellet was stored at -20°C under argon.
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bioRxiv - Cell Biology 2024Quote: ... Cells were washed 3 times in wash buffer and incubated in CY5-conjugated antibodies at 1:300 and 0.6 mg/mL Hoescht-33342 (Invitrogen) for one hour with rocking at 4°C ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were collected into fluorescence-activated sorting tubes and co-stained with 5 µM 7AAD (Invitrogen) and 1X BD Pharmingen™ PE Annexin V (BD Biosciences ...
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bioRxiv - Cell Biology 2024Quote: ... and scraped with 300 µL extraction buffer 1:1 LC-MS methanol:water (Optima®, Thermo Fisher Scientific) into a 1.5 mL microcentrifuge tube ...
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bioRxiv - Cancer Biology 2024Quote: ... washed with Dulbecco’s phosphate-buffered saline (DPBS) 1X (Biowest) and detached enzymatically-TrypLE (Thermo Fisher). Cell suspension was collected to 15mL centrifuge tubes ...
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bioRxiv - Cancer Biology 2024Quote: Cells were washed with ice-cold PBS three times and treated with 1-5mM disuccinimidyl suberate (DSS, A39267, Thermo Scientific, Waltham, MA) for 30 min at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were cultured in the presence of 2.5 µM BrdU for one population doubling before 0.2 µg/ml colcemid (Gibco 15212012) treatment for the final 4 hours before trypsinization ...
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bioRxiv - Cancer Biology 2024Quote: Adherent cells grown on 13 mm circular glass coverslips (Thermo Fisher Scientific) were pre-extracted in 0.2% Triton x-100 in PBS for 2 min on ice ...
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bioRxiv - Cancer Biology 2024Quote: ... Both types of media were supplemented with 10% FBS (Gibco) and maintained in a humidified atmosphere at 37°C in 5% CO2 ...
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bioRxiv - Cancer Biology 2024Quote: ... 1mL of highly concentrated bacterial culture was seeded into 9mL of Middlebrook 7H9 + 10% ADC + Hygromycin 50μg/mL in 30mL sterile bottles (Cat. No. 2019-0030 – Thermo Scientific) and incubated at 37°C ...
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bioRxiv - Cancer Biology 2024Quote: ... whereas siRNA was delivered using Lipofectamine™ RNAiMAX (ThermoFisher Scientific) according to the manufacturer’s protocols ...
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bioRxiv - Cancer Biology 2024Quote: ... and Bcl-xS antibody was from ThermoFisher Scientific (Waltham ...
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bioRxiv - Developmental Biology 2024Quote: ... including IMDM (Thermo Fisher, No.12440053) and Ham’s F12 (Corning ...