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Citations for Thermo Fisher :
751 - 800 of 10000+ citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... The Plexin-B2 lock mutant expressing lentiviruses were transduced into GBM cells carrying CRISPR/Cas9 Plexin-B2 KO and were selected with 200 µg/ml G418 (Gibco) for 7 days before confirmation of expression by WB and ICC.
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bioRxiv - Cancer Biology 2024Quote: ... the cells were also counterstained for F-actin with Alexa-594 phalloidin (Thermo Fisher).
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bioRxiv - Cancer Biology 2024Quote: ... and penicillin–streptomycin (1:100; Gibco). Cells were propagated in adherent conditions on culture dishes coated with laminin (10 µg/ml in PBS ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were resolved by SDS-PAGE on 4–12% polyacrylamide NuPAGE gels (Invitrogen) and transferred onto nitrocellulose or PVDF membranes (Li-Cor Biosciences ...
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bioRxiv - Cancer Biology 2024Quote: ... Chips stained with CellMask (1/50,000 dilution in PBS, Thermo Fisher) were permeabilised with 0.1% Triton X-100 (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2024Quote: ... Secondary antibodies were incubated in dilution buffer at room temperature for 1 hr together with nuclear stain (DAPI, 1:1000; Thermo Fisher). For most preparations ...
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bioRxiv - Cancer Biology 2024Quote: ... was used with an Alexa Fluor 555 goat anti-rabbit secondary antibody (1/1000 dilution, Invitrogen A21429). Chips stained with CellMask (1/50,000 dilution in PBS ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein concentrations were determined using a BCA assay (Thermo Scientific). Proteins were resolved by SDS-PAGE on 4–12% polyacrylamide NuPAGE gels (Invitrogen ...
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bioRxiv - Cancer Biology 2024Quote: ... the microchannels were coated with laminin (100 µg/ml in PBS; Invitrogen) for 1 hour at 37 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were propagated in adherent conditions on culture dishes coated with laminin (10 µg/ml in PBS; 1 hour at 37 °C; Gibco). Passaging of GSCs was performed by dissociating cells with Accutase (BD Biosciences).
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bioRxiv - Cancer Biology 2024Quote: ... primary samples analyzed on an Exactive (Thermo Fisher Scientifc) operating in negative ion mode55 ...
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bioRxiv - Cancer Biology 2024Quote: Crude protein extraction obtained as described above was electrophoresed through a slab isoelectric focusing gel (pH 3-7, Invitrogen Novex EC66452) employing freshly made cathode and anode buffers (Novex) ...
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bioRxiv - Cancer Biology 2024Quote: ... MW 10,000 (Invitrogen D22910). After a wash with PBS ...
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bioRxiv - Cancer Biology 2024Quote: ... and proteins were detected by enhanced chemiluminescence (ThermoFisher Scientific Inc., cat#7074). Antibodies used in this study are listed in Supplementary Table 1.
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bioRxiv - Cell Biology 2024Quote: ... EGFP constructs transfection were performed using Lipofectamine® 2000 (Invitrogen). For SMARCA2 or SMARCA4 knockout ...
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bioRxiv - Cell Biology 2024Quote: ... A fraction of cDNA was mixed with TaqMan qPCR Master Mix (ThermoFisher, cat# 4444557), loaded into fill reservoir of the card and the reaction run on the ViiA™ 7 instrument ...
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bioRxiv - Cell Biology 2024Quote: ... 1-2 ug of total RNA was DNaseI (Ambion) digested followed by first strand syntheses using random hexamers and Superscript II reverse transcriptase (Invitrogen) ...
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bioRxiv - Cell Biology 2024Quote: ... The PEC was flatmounted with glass coverslips and mounting medium (Invitrogen, TA-030-FM). The stained samples were imaged on a ZEISS LSM 900 with Airyscan ...
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bioRxiv - Cell Biology 2024Quote: ... TetraSpeck™ Microspheres (Invitrogen, 0.1 μm) were used as CLEM fiducials ...
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bioRxiv - Cell Biology 2024Quote: ... Reactions were run with TaqMan Universal PCR Master Mix on the ViiA7 system (Applied Biosystems) according to the instructions of the manufacturer.
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bioRxiv - Cell Biology 2024Quote: ... PCR reactions were prepared in triplicate with Sybr Green Master Mix (Applied Biosystems) and amplified using a CFX96 PCR Cycler (Bio-rad) ...
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bioRxiv - Cell Biology 2024Quote: ... Preparations were confirmed labeled through visual inspection of solution and through Nanodrop quantification (ThermoFisher).
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bioRxiv - Cell Biology 2024Quote: ... Non-specific binding was inhibited by incubating for 2 h in blocking buffer (5% BSA or Goat Serum (Invitrogen, #500622) + Triton X-100 (VWR ...
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bioRxiv - Cell Biology 2024Quote: ... cells were grown on 4-well glass chamber slides coated with poly-D-lysine (Thermo Fisher Scientific, Waltham, MA, USA). About 70,000 cells were seeded in each chamber well with a total aliquot of growth medium of 1 ml ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were counterstained with DAPI (0.25 μg/ml) for 2 min and embedded in Prolong Gold (Invitrogen, Carlsbad, CA, USA). When staining for α-tubulin and actin ...
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bioRxiv - Cell Biology 2024Quote: Custom TaqMan Array Cards were ordered from ThermoFisher (cat# 4391526). Total RNA (1 μg/sample ...
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bioRxiv - Cell Biology 2024Quote: ... in blocking buffer followed by PBS wash 15 min three times and then incubation for 2 h at room temperature with Alexa Fluor 594 conjugated donkey anti-rabbit antibody (1:1000, ThermoFisher A-21207). Stained samples were then washed for 15 min three times in PBS ...
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bioRxiv - Cell Biology 2024Quote: ... was fluorescently labeled using Ulyssis Alexa Fluor 647 Nucleic Acid Labeling Kit (Thermo Fisher, U21660). In short ...
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bioRxiv - Cell Biology 2024Quote: ... and 600 μg/ml L-glutamine (Gibco; Thermo Fisher
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bioRxiv - Cell Biology 2024Quote: ... Total RNA (1 μg/sample) was reverse transcribed to cDNA using SuperScript™ IV VILO™ Master Mix (ThermoFisher, cat# 11756050). A fraction of cDNA was mixed with TaqMan qPCR Master Mix (ThermoFisher ...
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bioRxiv - Cell Biology 2024Quote: ... immunofluorescence staining for ZO-1 (Thermo Fisher Scientific, Waltham, MA #33-9111) and BEST1 (Novus ...
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bioRxiv - Cell Biology 2024Quote: ... cDNA was amplified with platinum Taq polymerase (Invitrogen) for RERE (30 cycles ...
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bioRxiv - Cell Biology 2024Quote: ... and 600 μg/ml L-glutamine (Gibco; Thermo Fisher
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bioRxiv - Cell Biology 2024Quote: ... diluted in blocking buffer containing Hoechst 33342 (1:1000, ThermoFisher 33342) was added to the tissue for 48 h at 4ºC with gentle rocking ...
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bioRxiv - Cell Biology 2024Quote: ... digested followed by first strand syntheses using random hexamers and Superscript II reverse transcriptase (Invitrogen). A list of primers for which amplicons were confirmed for specificity by Sanger sequencing are presented in Supplementary Table 2 ...
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bioRxiv - Cell Biology 2024Quote: ... GAPDH (for in vitro) or 18s (for in vivo) as control (Applied Biosystems). Reactions were run with TaqMan Universal PCR Master Mix on the ViiA7 system (Applied Biosystems ...
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bioRxiv - Cell Biology 2024Quote: ... RNA was reverse transcribed using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Foster City, CA). Real-time PCR was performed using FAM-labeled TaqMan probes targeting genes of interest and a VIC-labeled TaqMan probe targeting β-actin ...
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bioRxiv - Cell Biology 2024Quote: ... slides were washed five times in 0.01% TBST and covered with TBST containing appropriate secondary antibody in 1:300 dilution (goat anti-mouse IgG Alexa Fluor 488, goat anti-rabbit IgG Alexa Fluor 594 (Invitrogen, Carlsbad, CA, USA)) and incubated for 40 min at RT in the dark in a humidified chamber ...
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bioRxiv - Cell Biology 2024Quote: ● 90 mm Petri Dishes (Fisher scientific).
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bioRxiv - Cell Biology 2024Quote: ... cell selection was undertaken using 4 µg/mL puromycin dihydrochloride (Gibco). ELOVL5 upregulation was confirmed by Western blot as described previously27.
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bioRxiv - Cell Biology 2024Quote: ... secondary antibodies were diluted 1:300 in Abdil together with 0.3 μg/mL Hoescht-33342 (Invitrogen) and the sample was incubated for 1 h at room temperature followed by 3 consecutive washes in PBS containing 0.1% Triton X-100 ...
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bioRxiv - Cell Biology 2024Quote: ... and reversed transfected at a concentration of 5 nmol/L using Lipofectamine RNAiMAX transfection reagent (Invitrogen) according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2024Quote: ... SelectaTM ultrasons-H (Fisher scientific).
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bioRxiv - Cell Biology 2024Quote: ● Disposable 1 mL cuvettes (Fisher scientific).
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bioRxiv - Cell Biology 2024Quote: ... Cells were collected into fluorescence-activated sorting tubes and stained with 2.5 µM MitoSOX™ Red Mitochondrial Superoxide Indicator (Invitrogen) in PBS for 30 mins at 37 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... Total RNA was extracted from live cells using Trizol reagent (Invitrogen) and purified by Direct-zol RNA Purification Kit (Zymo) ...
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bioRxiv - Cell Biology 2024Quote: ... Alexa Fluor-568 conjugated anti-rabbit IgG (1:1000) antibody (ThermoFisher, Massachusetts, USA) or Hoechst 33342 (diluted into 1 µg/ml ...
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bioRxiv - Developmental Biology 2024Quote: ... on a StepOnePlus System (Thermo Fisher Scientific). The relative expression of gene was calculated as 2–ΔΔCt using the comparative Ct (ΔΔCt ...
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bioRxiv - Developmental Biology 2024Quote: ... RNA (2 μg) was reverse transcribed with a SuperScript III Reverse Transcription kit (Applied Biosystems, Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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“Identification of microRNAs regulated by E2F transcription factors in human pluripotent stem cells”bioRxiv - Developmental Biology 2024Quote: ... primary antibody from Santa Cruz Biotechnology (sc-5279) and Alexa Fluor 488 goat anti-mouse IgG (1:400) from Invitrogen (A11001) were used ...