Labshake search
Citations for Thermo Fisher :
5151 - 5200 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2024Quote: ... was purchased from American Type Culture Collection (ATCC) and cultured in Dulbecco’s minimal essential medium (MEM) supplemented with 10% fetal bovine serum (FBS) (Invitrogen) and 1% penicillin/streptomycin and L-glutamine ...
-
bioRxiv - Microbiology 2024Quote: ... A 1 mL suspension of the bacterial cells was mixed with EZ-Link Sulfo-NHS-LC-Biotin (Thermo Fisher Scientific, #A39257) dissolved in 100 µl of sterile MilliQ water ...
-
bioRxiv - Microbiology 2024Quote: ... Incubation mixtures were arranged in 96-well plates and introduced into the StepOne Real-Time PCR equipment (Thermo Fisher Scientific). Samples were heated from 10 °C to 80 °C and fluorescence was measured at each point with SYBR Green filters ...
-
bioRxiv - Microbiology 2024Quote: ... and XhoI (Thermo Fisher) restriction enzymes ...
-
bioRxiv - Microbiology 2024Quote: ... 50 µL of Dynabeads™ M-280 Sheep Anti-Rabbit IgG (Thermo Fisher Scientific, #11203D) was added to each tube followed by incubation at 4°C for 2 hours ...
-
bioRxiv - Microbiology 2024Quote: ... 20 μL of pre-washed Streptavidin Dynabeads (Thermo Fisher Scientific, #11205D) was added to each sample and incubated for 48 hours ...
-
bioRxiv - Microbiology 2024Quote: ... pilosicoli were first labeled with Alexa Fluor 680 (Invitrogen, #A37574) and then subjected to a one-hour pre-incubation with a 1:25 dilution of anti-BPP43_05035 or control serum at RT ...
-
bioRxiv - Microbiology 2024Quote: ... secondary antibody or streptavidin Alexa Fluor 680 (1:20,000; Invitrogen, #S21378) for one hour at RT in the dark ...
-
bioRxiv - Microbiology 2024Quote: ... We used dissociation enzyme 0.25% Trypsin (Thermo Fisher Scientific) and 10 ug/mL DNase I (Sigma ...
-
bioRxiv - Microbiology 2024Quote: ... #CCL-247) cells were cultured in HyClone IMDM Modified medium (Cytiva, #SH30228.01) supplemented with 10% fetal bovine serum (FBS; Thermo Fisher Scientific, #10270106) in a 5% CO2 humidified atmosphere at 37°C ...
-
bioRxiv - Microbiology 2024Quote: ... the cells were stained using anti-rabbit IgG Alexa Fluor 488 (Invitrogen, #A21206) at a 1:300 dilution for two hours at RT ...
-
bioRxiv - Microbiology 2024Quote: Caco-2 cells were cultured and stained on chamber slides (Thermo Fisher Scientific, #154534PK). Cells were washed 3 times in PBS followed by a 10-minute fixation in 4% PFA in PBS at RT ...
-
bioRxiv - Microbiology 2024Quote: ... were grown in Luria broth (Invitrogen, #12795084) with 30 µg/ml kanamycin at 37°C.
-
bioRxiv - Cell Biology 2024Quote: Total RNA was extracted from lungs using Trizol (Invitrogen) and RNeasy Mini RNA extraction kit (Qiagen) ...
-
bioRxiv - Cell Biology 2024Quote: ... 10 μl CTB488 (concentration at 1 mg/ml, Thermo Scientific, C22841) was injected into the dorsal side of the trachea from bilateral sides ...
-
bioRxiv - Cell Biology 2024Quote: ... Resuspend in 50-500 µL of 1.11x tagmentation buffer (66 mM Tris-acetate (pH=7.8, BP-152, Thermo Fisher Scientific), 132 mM K-acetate (P5708 ...
-
bioRxiv - Microbiology 2024Quote: ... dissolved in PBS with 5% Fetal Bovine Serum (FBS, GIBCO) to digest the tissues ...
-
bioRxiv - Microbiology 2024Quote: ... sections were treated with Alexa Fluor 488 goat anti-rabbit (1:1000; Invitrogen, #A11055) and Alexa Fluor 555 donkey anti-mouse (1:1000 ...
-
bioRxiv - Microbiology 2024Quote: ... qRT-PCR was performed on an ABI StepOnePlus PCR system (Thermo Fisher Scientific), and results were normalized to GAPDH mRNA levels ...
-
bioRxiv - Microbiology 2024Quote: Isolation of primary embryonic neurons and single-cell suspensions was performed using Pierce Primary Neuron Isolation Kit (88280, Thermo Scientific) by density gradient centrifugation.
-
bioRxiv - Microbiology 2024Quote: Proteins were subjected to reduction in 4X reducing sample buffer (8% SDS, 400 mM Dithiothreitol) and resolved on a precast 4%–12% SDS-polyacrylamide gel (Thermo Fisher Scientific, #XP04125BOX). Coomassie staining was performed using Imperial Protein Stain (Thermo Fisher Scientific ...
-
bioRxiv - Microbiology 2024Quote: ... Cell suspensions were filtered using a 40-μm nylon cell strainer and red blood cells were removed by red blood cell lysis solution (Thermo Fisher Scientific). Dissociated cells were washed with DPBS containing 2% FBS ...
-
bioRxiv - Cell Biology 2024Quote: ... and 1 mM sodium pyruvate (Gibco). RPE1-hTERT cells were grown in DMEM/F12 medium (Gibco ...
-
bioRxiv - Cell Biology 2024Quote: ... RPE1-hTERT cells were grown in DMEM/F12 medium (Gibco) supplemented with 10% FBS (Gemini Bio) ...
-
bioRxiv - Developmental Biology 2024Quote: ... chicken anti-GFP (1:1000, A10262, Invitrogen/ Thermo Fisher), rabbit anti-pHH3 (1:200 ...
-
bioRxiv - Developmental Biology 2024Quote: ... while the proliferation assay was conducted employing the BrdU incorporation method (Invitrogen, #000103), following previously established protocols [46] ...
-
bioRxiv - Developmental Biology 2024Quote: EdU staining was performed according to the Click-iT EdU Cell Proliferation Kit manufacturer’s instructions (Thermo Fisher Scientific; C10340). Briefly ...
-
bioRxiv - Developmental Biology 2024Quote: ... and 100:100 units:µg/ml penicillin: streptomycin (Sigma, P4333; or Gibco, 15140122), supplemented with leukemia inhibitory factor (LIF ...
-
bioRxiv - Cell Biology 2024Quote: ... RPL11 (Invitrogen, 37-3000), RPL5 (Invitrogen ...
-
bioRxiv - Developmental Biology 2024Quote: ... 15% ESC-qualified fetal bovine serum (Invitrogen), 2 mM L-glutamine (Invitrogen ...
-
bioRxiv - Neuroscience 2024Quote: ... All images were acquired using an EVOS M5000 micro-scope (Invitrogen). The microscope was equipped with DAPI (AMEP4650) ...
-
The satiety hormone cholecystokinin gates reproduction in fish by controlling gonadotropin secretionbioRxiv - Neuroscience 2024Quote: All expression plasmids were generated using the Tol2kit (63) and Gateway system (Invitrogen). Briefly ...
-
bioRxiv - Cell Biology 2024Quote: ... RPL5 (Invitrogen, PA5-102539), RPL17 (Gentech ...
-
bioRxiv - Developmental Biology 2024Quote: ... and concentration was determined using Qubit™ dsDNA HS Assay kit (Invitrogen, Q32854). Each library was diluted to 4 nM and pooled for sequencing on an Illumina Hiseq4000 ...
-
bioRxiv - Cell Biology 2024Quote: ... and glycogen (Thermo Scientific, R0551) and re-suspended in a buffer containing 20 mM Tris-HCl pH 7.5 ...
-
bioRxiv - Developmental Biology 2024Quote: ... and QuantStudio® 5 System (Applied Biosystems, USA). Equimolar pooling of libraries was performed based on QC values ...
-
bioRxiv - Developmental Biology 2024Quote: ... All murine ESC lines were cultured on mitotically inactivated C57BL/6J mouse embryonic fibroblasts (MEFs) in standard ESC culture medium composed of knock out DMEM (Invitrogen, 10829-018), 15% ESC-qualified fetal bovine serum (Invitrogen) ...
-
bioRxiv - Developmental Biology 2024Quote: ... cDNA conversion was performed using SuperScript-III RT kit (Thermo Fisher; 18080093) according to manufacturer’s instructions using 1 μg of extracted RNA ...
-
bioRxiv - Developmental Biology 2024Quote: ... 2 mM L-glutamine (Invitrogen, 25030-024), 0.1 mM 2-mercaptoethanol (Invitrogen ...
-
bioRxiv - Cell Biology 2024Quote: ... 200 ng of each sample were loaded onto a 300 µm ID × 5mm PepMap C18 pre-column (Thermo Scientific, Dionex) at 20 µL/min in 2% ACN ...
-
bioRxiv - Developmental Biology 2024Quote: ... 0.1 mM 2-mercaptoethanol (Invitrogen, 31350-010), and 100:100 units:µg/ml penicillin ...
-
bioRxiv - Developmental Biology 2024Quote: ... Each anterior- posterior or lateral section was then collected for total RNA extraction with the RNAqueous- Micro Total RNA Isolation Kit (Thermo Fisher Scientific, # AM1931) according to the kit’s protocol and processed for 3’RNA-seq library preparation ...
-
bioRxiv - Developmental Biology 2024Quote: ... Carcasses were then placed in 25mg/mL 10kD fluorescein-conjugated dextran (Thermo Fisher, D1820) diluted 1:8 in Schneider’s medium and incubated for 30min at room temperature in the dark while rocking ...
-
bioRxiv - Developmental Biology 2024Quote: ... the tissue slurry was further digested by collagenase II (100U/ml) and dispase (1.1U/ml, Gibco) in wash medium for 0.5 h to get single cell suspension for cell sorting ...
-
bioRxiv - Developmental Biology 2024Quote: ... stained with DAPI (1μg/ml in 0.05% PBT) and mounted in anti-fade diamond solution (Invitrogen). tdT fluorescence was preserved ...
-
bioRxiv - Developmental Biology 2024Quote: ... 1X Non-essential AA (Gibco), Pen/Strep 1X (Gibco)) ...
-
bioRxiv - Developmental Biology 2024Quote: ... anti-mouse antibody at 1:1000 dilution and all histone marks with secondary donkey Alexa Fluor 546 (Thermofisher) anti-rabbit at 1:1000 dilution ...
-
bioRxiv - Developmental Biology 2024Quote: ... and quantified using Qubit Fluorimetric Quantification (Thermo Scientific). The integrity of the RNA was determined using the TapeStation High Sensitivity RNA kit (Agilent Technologies) ...
-
bioRxiv - Developmental Biology 2024Quote: ... Protein G Dynabeads (Thermo Scientific Catalog#10003D) were washed ...
-
bioRxiv - Developmental Biology 2024Quote: ... then mounted in ProLong Diamond (Thermofisher) and left to cure overnight in the dark at 4 °C ...