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Citations for Thermo Fisher :
5301 - 5350 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2024Quote: ... The samples were then further digested by pipetting up and down with 0.25% trypsin (GIBCO Cat# 15090-046), in 1mM EGTA and 0.1 mg/ml of Polyvinyl alcohol dissolved in PBS for 1 min at 37°C in a waterbath ...
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bioRxiv - Genomics 2024Quote: ... cells were stained with DAPI in a 0.5μg/mL concentration (Invitrogen, D1306) at 4°C for 3 min immediately before running the samples in the flow cytometer ...
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bioRxiv - Genetics 2024Quote: ... Purified PCR products were eluted in 25 µl sterile H2O and quantified using a dsDNA broad range assay kit on a Qubit fluorometer 3.0 (both Invitrogen). Sequencing libraries were constructed following ONT’s ’Amplicon barcoding with Native Barcoding’ protocol (version ...
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bioRxiv - Genomics 2024Quote: ... cells were dissociated using 500uL of prewarmed TrypLE (ThermoFisher 12604013) and left for 5 min at 37℃ ...
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bioRxiv - Genomics 2024Quote: ... and then treated with 400µg/ml RNase A (Fisher Scientific) for 30 mins at 37°C in a humidity chamber ...
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bioRxiv - Genomics 2024Quote: ... 22] on the Ion S5 DNA sequencing platform (Thermo Fisher Scientific), which in turn involved PCR amplification ...
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bioRxiv - Genomics 2024Quote: ... and 20X Salmon Sperm DNA (Invitrogen) and resuspended at 100ng/ul in TE buffer.
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bioRxiv - Genetics 2024Quote: ... Extracted samples were treated with TURBO DNA-free™ Kit (Catalog Number: AM1907, ThermoFisher Scientific) according to manufacturer’s instructions ...
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bioRxiv - Genetics 2024Quote: ... mounted using ProLongTM Gold Antifade aqueous mounting medium (ThermoFisher Scientific, catalog # P36930) and coverslips placed and sealed with nail polish ...
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bioRxiv - Genetics 2024Quote: ... Nuclei quality and concentration were measured in Countess AutoCounter (Invitrogen, C10227). Diluted nuclei were loaded and incubated in chromium single cell ATAC library & gel bead kit’s transposition mix (10X Genomics ...
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bioRxiv - Genetics 2024Quote: ... 293T cells (Life Technologies, Carlsbad, CA, USA) were seeded in five 15 cm2 dishes with 13-15×106 cells per plate 24 hours pre-transfection ...
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bioRxiv - Genetics 2024Quote: Unfixed bone marrow smears were diffusely spread on Fisherbrand™ SuperfrostTM glass slides (Fisher Scientific, catalog # 12-550-14G) without heparin treatment in the dark ...
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bioRxiv - Genetics 2024Quote: ... and 1 mM Ethylenediaminetetraacetic acid [EDTA]) containing 0.01% MilliporeSigma™ GelRed™ Nucleic Acid Stain (Fisher Scientific, catalog # SCT123) with electrophoresis performed at 200V for 30 minutes to detect the 604 bp transgene amplicon ...
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bioRxiv - Genetics 2024Quote: Total RNA was extracted using TRIzol (Fisher Scientific) from pellets of mixed-stage animals collected from non-starved but crowded plates in biological triplicate for each strain after exposure to either unc-22 RNAi or the L4440 vector ...
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bioRxiv - Genetics 2024Quote: ... sections were treated with 500-1000 µL of 1:500 dilution (4 µg/mL) of goat anti-hamster IgG (H+L) AlexaFluor® 488 antibody (ThermoFisher Scientific catalog # A78963) diluted in 2% NGS in 1X PBS and incubated for 1 hour at RT in the dark ...
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bioRxiv - Genetics 2024Quote: ... DNA concentrations were measured using Nanodrop 2000 (Thermo Fisher Scientific).
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bioRxiv - Genetics 2024Quote: Neutral lipids in one-day-old synchronized adults were visualized by Oil Red O dye (Thermo Fisher Scientific, USA) staining using a published protocol [77] ...
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bioRxiv - Genetics 2024Quote: Bodipy 493/503 (Catalog Number: D3922, Invitrogen) was used to stain lipid droplets in age synchronized worms as described earlier [78] ...
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bioRxiv - Genomics 2024Quote: ... Probes were then precipitated with 20X Mouse Cot-1 DNA (Invitrogen) and 20X Salmon Sperm DNA (Invitrogen ...
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bioRxiv - Genetics 2024Quote: ... and PCRs were carried out using AmpliTaq DNA polymerase (Life Technologies). Primers and PCR protocols are available on request ...
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bioRxiv - Genomics 2024Quote: ... as evident from NanoDrop spectrophotometry and Qubit fluorometry (both the operations were carried out using platforms manufactured by Thermo Fisher Scientific), were used as the input material (approximately 1 ng total quantity ...
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bioRxiv - Genomics 2024Quote: ... The resulting biotin-marked fragments were isolated using Dynabeads MyOne Streptavidin C1 (Invitrogen #65001) and diluted in TWB buffer (1M Tris-HCl pH 8.0 ...
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bioRxiv - Genomics 2024Quote: ... viability was corroborated by Calcein AM (Thermo Fisher Scientific, Cat# C1430) and Draq7TM (Cat# 564904 ...
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bioRxiv - Genetics 2024Quote: ... 1 μg RNA was reverse transcribed using the TaqMan Reverse transcription kit and gene level detection performed using SYBR Green Master Mix (all Thermo Scientific). Relative expression was normalized to GAPDH and ACTB ...
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bioRxiv - Developmental Biology 2024Quote: ... embryos were harvested at the 2-cell stage on E1.5 by flushing the oviducts with M2 Media (Cytospring) and each cell was microinjected with 100ng/ul Cas9 mRNA (made by IVT (mMESSGAE mMACHINE SP6 transcription kit, Thermo Fisher) using Addgene plasmid 122948) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and 100 U/mL penicillin-streptomycin (PS; Gibco).
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bioRxiv - Developmental Biology 2024Quote: ... onto a 1% (v/v) gel coated wells (Geltrex (Thermo Fisher Scientific), or with Cultrex SCQ (Bio-techne)) ...
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bioRxiv - Developmental Biology 2024Quote: ... tagBFP PCR-fused to nkx3.1 or cxcl12b were made by amplification using primers in S4 Table and cloned into pDONR221 using BP Clonase (ThermoFisher). Transposon Tol2 vectors were assembled using LR Clonase (ThermoFisher ...
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bioRxiv - Developmental Biology 2024Quote: ... The cDNA was synthesized from total RNA (1μg) using High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems). Quantitative real-time RT-PCR (qPCR ...
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bioRxiv - Developmental Biology 2024Quote: Total RNA was isolated from Ino80WT and Ino80cKO spermatocytes using Trizol reagent (Invitrogen) followed by the Direct-zol RNA kit (Zymo) ...
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bioRxiv - Developmental Biology 2024Quote: ... and mounted in Prolong Gold anti-fade medium (P-36931; Life Technologies). Relative signal intensity was measured by NIH ImageJ software ...
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bioRxiv - Developmental Biology 2024Quote: ... or 4% normal donkey serum (Gibco) in PBS 1 hour to overnight at 4°C ...
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bioRxiv - Developmental Biology 2024Quote: ... After incubation with Alexa Fluor 488-conjugated goat anti-rat IgG secondary antibody (1:200, Thermo Fisher) for 30 min ...
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bioRxiv - Biochemistry 2024Quote: ... The digestion was quenched to 0.5% trifluoracetic acid and spiked with Pierce Retention Time Calibrant (PRTC) peptide cocktail (Thermo Fisher Scientific) to a final concentration of 50 fmol/µL ...
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bioRxiv - Developmental Biology 2024Quote: ... Cas9 mRNA was transcribed from linearized pCS2-Cas9 plasmid using the mMachine SP6 kit (Ambion). To generate mutants ...
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bioRxiv - Developmental Biology 2024Quote: ... The samples were blocked in 4% heat-inactivated goat serum (Gibco) or 4% normal donkey serum (Gibco ...
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bioRxiv - Developmental Biology 2024Quote: ... or with Cultrex SCQ (Bio-techne) coated 6 well plates (Nunc). Cells were passaged as small clumps every 4 to 5 days with Dispase (Gibco) ...
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bioRxiv - Developmental Biology 2024Quote: ... and the protein concentration was measured using a NanoDrop spectrophotometer (Thermo Fisher). The FGF10-Alexa488 solution was stored in small aliquots at −20°C to avoid freeze-thaw cycles ...
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bioRxiv - Developmental Biology 2024Quote: ... Lung lobes were cut into pieces and soaked in dispase solution (1 U/mL in HBSS; Gibco) for 10 min at 37 °C ...
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bioRxiv - Developmental Biology 2024Quote: ... Alexa Fluor–conjugated WGA (membrane, Thermo Fisher Scientific). All samples were mounted on slides using 90% glycerol (in 1x PBS) ...
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bioRxiv - Developmental Biology 2024Quote: ... Transposon Tol2 vectors were assembled using LR Clonase (ThermoFisher) and the Tol2 vector (53) ...
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bioRxiv - Developmental Biology 2024Quote: Recombinant FGF10 protein was labeled using an Alexa Fluor 488 Microscale Protein Labeling Kit (Invitrogen). To reduce the loss of physiological activity of FGF10 ...
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bioRxiv - Developmental Biology 2024Quote: ... In short, samples (hPSC-cysts, hPSC-amnion) grown on the coverslip were rinsed with phosphate-buffered saline (PBS; Gibco) twice ...
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bioRxiv - Developmental Biology 2024Quote: ... Cells were passaged as small clumps every 4 to 5 days with Dispase (Gibco). All cells were cultured at 37°C with 5% CO2 ...
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bioRxiv - Developmental Biology 2024Quote: ... Protein A conjugated Dynabeads (Invitrogen), and for mouse antibodies ...
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bioRxiv - Developmental Biology 2024Quote: 30 hpf MZ nkx3.1ca116 mutant and wild type embryos were collected and RNA prepared using Trizol (ThermoFisher, Waltham MA). Libraries were prepared from 50ng/µg/µl of total mRNA per sample using the Illumina Ultra II directional RNA library prep (SanDiego ...
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bioRxiv - Developmental Biology 2024Quote: ... hESC were cultured on 1% (v/v) Geltrex (Thermo Fisher Scientific), or with Cultrex SCQ (Bio-techne ...
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bioRxiv - Biochemistry 2024Quote: ... HALT cocktail (protease and phosphatase inhibitors, Thermo Fisher Scientific) was added to plasma (20-100 µL ...
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bioRxiv - Developmental Biology 2024Quote: ... the unreacted dye in the sample was removed using a Slide-A-Lyser Mini Dialysis Unit (Thermo Scientific) in PBS for 16 h at 4°C ...
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bioRxiv - Developmental Biology 2024Quote: ... sgRNAs were generated by in vitro transcription using the Megascript kit (Ambion). Cas9 mRNA was transcribed from linearized pCS2-Cas9 plasmid using the mMachine SP6 kit (Ambion) ...