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Citations for Thermo Fisher :
5401 - 5450 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: Equipment: Attune NXT flow cytometer (Thermo fisher scientific A29002). Neon transfection system (Thermo Fischer MPK5000).
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bioRxiv - Molecular Biology 2024Quote: ... 1 µg of the extracted total RNA per sample was reverse transcribed using oligo(dt)18 primers (First Strand cDNA Synthesis Kit; ThermoFisher Scientific; Waltham, USA), and 1 µL cDNA was used for qPCR with tgb-1 as housekeeping gene (19) ...
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bioRxiv - Molecular Biology 2024Quote: ... and first-strand cDNA synthesis was performed using Superscript III (Invitrogen). Cells were cultured in phenol red-free medium supplemented with 5% FBS-charcoal stripped for 24 hours then treated with 10 nM 17β-estradiol for either 1 ...
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bioRxiv - Neuroscience 2024Quote: ... magnesium or phenol red (HBSS) (Gibco/Thermo Fischer Scientific, U.S.), pH 7.0-7.4 ...
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bioRxiv - Neuroscience 2024Quote: ... saline-perfused brains were collected into ice-cold Roswell Park Memorial Institute 1640 medium without phenol red (Gibco/Thermo Fischer Scientific, U.S.) and stored at 4 °C for a maximum of 3 h until processing.
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bioRxiv - Neuroscience 2024Quote: ... Alexa Fluor-conjugated secondary antibodies (Invitrogen, Molecular Probes) applied overnight at 1:200 dilutions in 0.2 % PBST included anti-rabbit (488 nm) ...
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bioRxiv - Neuroscience 2024Quote: ... Primary antibody incubation was followed by Alexa-488-conjugated donkey anti-rabbit secondary antisera (1:500; Invitrogen).
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bioRxiv - Neuroscience 2024Quote: ... DyLightTM 405 conjugated (21831 Thermoscientific) or Avidin-FITC (43-4411 Invitrogen). TTX was purchased from Alomone Labs ...
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bioRxiv - Neuroscience 2024Quote: ... Alexa Fluor-conjugated secondary antibodies (Invitrogen, Molecular Probes) applied overnight at 1:200 dilutions in 0.2 % PBST included anti-rabbit (488 nm) ...
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bioRxiv - Neuroscience 2024Quote: Fixed brains were coronally sectioned on a Microm HM450 freezing microtome (Thermo Scientific, U.S.) with 35 µm thickness in series of 12 and stored in Walter’s antifreeze solution at 4 °C until IHC staining ...
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bioRxiv - Neuroscience 2024Quote: ... with LR Clonase II Plus (Invitrogen). Verified constructs (25 ng/μl plasmid DNA ...
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bioRxiv - Neuroscience 2024Quote: ... Tie2 (Thermo Fisher, Cat#14–5987-81) 6C3 (BioLegend ...
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bioRxiv - Neuroscience 2024Quote: ... in nuclease-free water (Thermo Fisher Scientific, Cat#: 10977023) according to a modified SmartSeq2 protocol46,50 ...
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bioRxiv - Neuroscience 2024Quote: ... total RNA was purified using the RNA Mini Kit (Invitrogen, Waltham MA). For quantitative polymerase chain reaction (qPCR) ...
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bioRxiv - Neuroscience 2024Quote: ... PDPN (Thermo Fisher Scientific, Cat#17-9381-42), CD164 (BioLegend ...
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bioRxiv - Molecular Biology 2024Quote: ... Nikkyo Technos) using EASY-nLC 1200 system (ThermoFisher Scientific). The elution conditions are listed in Table S3 ...
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bioRxiv - Molecular Biology 2024Quote: ... The enteroids were then pelleted at 300 x g for 3 min at 4 °C and washed once with advanced DMEM/F-12 basal media (Gibco). The enteroid pellet was resuspended in the desired volume of IntestiCult complete medium (Stemcell Technologies ...
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bioRxiv - Molecular Biology 2024Quote: ... Primocin® was discontinued after the second passage and the enteroids were supplemented with Anti/Anti (Gibco). Enteroids were maintained and expanded in Matrigel patties in the “basal out” orientation.
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bioRxiv - Microbiology 2024Quote: ... Nuclei were stained with diamidino-2-phenylindole (DAPI; Life Technologies). Images were acquired on the LSM 980.
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bioRxiv - Microbiology 2024Quote: ... Events were assessed by flow cytometry on the AttuneNxT (Thermo Fisher Scientific). At least 10,000 events were collected per run ...
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bioRxiv - Microbiology 2024Quote: Total RNA from U2OS cells was isolated using TRIzol® reagent (Invitrogen). RNA integrity was assessed using the Bioanalyzer TapeStation 4200 RNA High Sensitivity (Agilent) ...
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bioRxiv - Microbiology 2024Quote: ... qRT-PCR was performed with PowerTrack SYBR Green Master Mix (Thermo Fisher Scientific) on the LightCycler 480 System (Roche ...
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bioRxiv - Microbiology 2024Quote: Total RNA was isolated using the PureLinkTM RNA Mini Kit (Invitrogen). RNA was reverse transcribed using SuperScript IV First-Stand Synthesis System (Invitrogen ...
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bioRxiv - Microbiology 2024Quote: ... Power SYBR Green PCR Master Mix (Applied Biosystems, Thermo Fisher) was used in 20 μl reactions with 10 ng of DNA and 50 nM primers ...
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bioRxiv - Molecular Biology 2024Quote: BHK-21 and MDBK cell based replicon replication assays were performed in 24-well plates with 0.5 µg/cm2 of RNA using Lipofectin transfection reagent (Life Technologies) as previously described (35) ...
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bioRxiv - Molecular Biology 2024Quote: ... Genomic DNA was digested with TURBO DNA-free (Ambion Turbo Dnase kit AM1907) according to manufacturer′s guidelines ...
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bioRxiv - Molecular Biology 2024Quote: ... S-fragment sequences containing deletions were chemically synthesised (Thermo Fisher Scientific) and inserted into the replicon vector using complementary EagI sites.
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bioRxiv - Molecular Biology 2024Quote: ... All transcripts were quantified by NanoDrop 1000 (Thermo Scientific) and RNA integrity assessed by 3-(N-morpholino ...
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bioRxiv - Molecular Biology 2024Quote: ... The RT reactions were performed with SuperScript IV reverse transcriptase (ThermoFisher, 18090010) following the manufacturer′s instructions using gene-specific primers ...
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bioRxiv - Molecular Biology 2024Quote: ... and residual genomic DNA was removed using DNA-free DNA removal Kit (Thermo Fisher Scientific) prior to ethanol precipitation ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.075 μL AmpErase Uracil N-Glycosylase (Thermo Fisher Scientific, MA, USA), and 2.0 μL DNA template in 1× TaqMan Environmental Master Mix (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2024Quote: Small RNA (<200 nt) was isolated directly from the pellets of infected RBCs using the mirVana miRNA isolation kit (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... on a Qubit® 4 fluorometer (Thermo Scientific™, Waltham, MA, USA). The DNA samples of 2-d incubation were used for isopycnic centrifugation ...
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bioRxiv - Microbiology 2024Quote: ... and Collibri Library Quantification Kit (Invitrogen).
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bioRxiv - Microbiology 2024Quote: ... PCR amplification for Illumina sequencing was performed using 2X Phusion High-Fidelity PCR Master Mix (Thermofisher Scientific) for 12 PCR cycles (98°C 5s ...
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bioRxiv - Microbiology 2024Quote: Acquired raw data files were analyzed using Proteome Discoverer v2.5 (Thermofisher Scientific) with Sequest HT search engine against Plasmodium falciparum PF3D7 protein database (SwissProt) ...
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bioRxiv - Microbiology 2024Quote: ... The sample was cleaned on a C18 Hypersep plate with 40 µL bed volume (Thermofisher Scientific), dried using a vacuum concentrator (Eppendorf) ...
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bioRxiv - Microbiology 2024Quote: ... 2 µg samples injected on a 50 cm long EASY-Spray C18 column (Thermofisher Scientific) connected to an Ultimate 3000 nanoUPLC system (Thermofisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... resuspended in 1ml FACS staining buffer containing 0.5 mM Sytox Green Nucleic acid stain (Life Technologies), and incubated for 5 min at RT in the dark ...
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bioRxiv - Molecular Biology 2024Quote: ... Anti-porin was bought from Thermofisher (Waltham, MA - USA) and anti-Cox2 from Abcam (Cambridge ...
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bioRxiv - Molecular Biology 2024Quote: C2C12 cells (Shanghai Cell Bank, Chinese Academy of Sciences, passages 3-8) were cultured in Dulbecco modified Eagle medium (DMEM) (Invitrogen, Carlsbad, CA, USA) supplemented with 10% fetal bovine serum (Gibco ...
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bioRxiv - Molecular Biology 2024Quote: ... The nuclear protein was extracted using NE-PER Nuclear and Cytoplasmic Extraction Reagents (ThermoFisher). Protein from conditioned media was extracted using methanol-choloroform precipitation method as previous (Jakobs et al. ...
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bioRxiv - Molecular Biology 2024Quote: ... qPCR was performed using ChamQ SYBR Color qPCR Master Mix (Vazyme) on a 7900 HT Real-time PCR system (384-cell standard block) (Applied Biosystems). Relative mtDNA was quantified by qPCR using primers for mitochondrially-encoded Nd1 normalized to nuclear-encoded 36B4 (Rplp0 ...
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bioRxiv - Molecular Biology 2024Quote: ... and 2.0 μL DNA template in 1× TaqMan Environmental Master Mix (Thermo Fisher Scientific). In all qPCR runs ...
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bioRxiv - Molecular Biology 2024Quote: Quantitative real-time PCR was performed in triplicate using two systems (change of equipment due to S.T. transfer): StepOnePlus Real-Time PCR system (Life Technologies, CA, USA; Exp. 1) or Light Cycler 96 system (Roche ...
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bioRxiv - Molecular Biology 2024Quote: ... Total RNA was extracted from clarified lysates using TRizol reagent (Thermo Fisher Scientific) and residual genomic DNA was removed using DNA-free DNA removal Kit (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... Reverse transcription was continued using Superscript III (Invitrogen) following manufacturers protocol with incubation at 52°C for 30 minutes.
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bioRxiv - Molecular Biology 2024Quote: ... RNA quality and concentration were determined by MOPS-formaldehyde denaturing agarose gel electrophoresis and Qubit RNA BR Assay Kit (Thermo Fisher Scientific).
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bioRxiv - Molecular Biology 2024Quote: ... Full length viral RNA was transcribed using a T7 MEGAscript kit (Thermo Fisher Scientific), DNase treated using TurboDNase (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... Mass spectra were acquired on a Q Exactive HF hybrid quadrupole orbitrap mass spectrometer (Thermofisher Scientific) ranging from m/z 375 to 1700 at a resolution of R=120,000 (at m/z 200 ...