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Citations for Thermo Fisher :
5251 - 5300 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... RNA was isolated from the cells using Trizol Reagent (#15596, TRIzol™ Reagent; Life Technologies, Thermo Fisher Scientific). RNA isolation was succeeded by cDNA preparation from 1µg of total RNA using iScript™ cDNA Synthesis Kit (#1708891 ...
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bioRxiv - Cell Biology 2024Quote: ... and 1 µl/ml of Halt Protease inhibitor cocktail (Thermo Scientific, 78430) dissolved in water ...
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bioRxiv - Immunology 2024Quote: ... Intracellular staining was done using the following antibodies: BLIMP1-PE (Invitrogen, Clone: 5E7), CXCL12-PE (R&D Biosystems ...
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bioRxiv - Biochemistry 2024Quote: RNAs were run for 1 hr at 180V in a 7M Urea 10% acrylamide gel and then transferred to a nylon membrane (Invitrogen) using XCell SureLock Mini-Cell system with the XCell II Blot Module (Invitrogen) ...
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bioRxiv - Biochemistry 2024Quote: ... Reaction mixtures were adjusted to a total volume of 5 μl (2 μl solution A; 1,5 μl solution B; 0,3 μl RiboLock (40 U/μl, Thermo Scientific); 0,2 μl D-Luciferin (10 mM ...
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bioRxiv - Biochemistry 2024Quote: ... AriB concentration was determined with Qubit Protein Broad Range Assay Kit (Invitrogen).
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bioRxiv - Biochemistry 2024Quote: ... was used as a template for T7 in vitro transcription with MEGAscript kit (Thermo Scientific). mRNA was treated with DNase I (Thermo Scientific ...
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bioRxiv - Biochemistry 2024Quote: ... using XCell SureLock Mini-Cell system with the XCell II Blot Module (Invitrogen). The membrane was cross-linked 5 min with UV ...
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bioRxiv - Biochemistry 2024Quote: ... mRNA was treated with DNase I (Thermo Scientific) and purified with Monarch PCR & DNA cleanup kit (NEB) ...
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bioRxiv - Biochemistry 2024Quote: ... RT-PCR using Power SYBR Green PCR Master Mix (Thermo Fisher Scientific, Waltham, MA) in a Quantstudio 12K Flex real time PCR system (Applied Biosystems) ...
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bioRxiv - Biochemistry 2024Quote: ... first-strand cDNA was synthesized using High-capacity cDNA reverse transcription kit (Thermo Fisher Scientific, Waltham, MA), and ...
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bioRxiv - Biochemistry 2024Quote: The sequences for Orc6 phosphorylation mutant Orc6-4A was obtained from Invitrogen GeneArt Synthesis (Thermo Fisher Scientific). This sequence was then used to replace the wild type Orc6 in the plasmid pJF18 as in Frigola et al. ...
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bioRxiv - Biochemistry 2024Quote: ... followed by 3-min permeabilization using 0.15% Surfact-Amps X-100 (28314, Thermo Fisher) in PBS ...
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bioRxiv - Biochemistry 2024Quote: ... were maintained in Sf-900™ II serum-free medium (Thermo Fisher) and used for baculovirus preparation and large-scale expression.
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bioRxiv - Biochemistry 2024Quote: ... coli strains used in this study include Mach1T1R (Thermo Fisher) and BL21 (DE3)T1R (Sigma) ...
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bioRxiv - Immunology 2024Quote: ... Cells were stained with LIVE/DEAD Fixable Blue cell stain (Thermofisher, L23105) for viability assessment and blocked with FcBlock with CD16/32 (Biolegend ...
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bioRxiv - Immunology 2024Quote: ... staining and mounting (ProLong Gold Antifade Moutant, ThermoFisher, P36930), the Zeiss Axioscan 7 microscope slide scanner was used to collect 20X images ...
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bioRxiv - Genetics 2024Quote: ... +15% iron-supplemented newborn bovine calf serum (Hyclone/Thermo Scientific, cat. #SH3007203) +10 ng/ml EGF +0.4 μg/ml hydrocortisone with added 1% penicillin/streptomycin ...
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bioRxiv - Genetics 2024Quote: ... supplemented with human keratinocyte growth supplement (HKGS, Gibco™, cat. #S0015), containing Human growth factor I insulin-like recombinant ...
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bioRxiv - Genetics 2024Quote: ... four days after injury both hind limbs were harvested and cultured as explants on an exosome-depleted FBS pre-coated dish with high-glucose DMEM (Gibco) and maintained at 37°C in a humidified incubator equilibrated with 5% CO2 ...
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bioRxiv - Genetics 2024Quote: ... After quality control analysis by Qubit 3.0 (Invitrogen) and a Bioanalyzer (Agilent) ...
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bioRxiv - Genetics 2024Quote: ... and 10 U/ mL Maxima H Minus Reverse Transcriptase (ThermoFisher)) were added ...
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bioRxiv - Genetics 2024Quote: ... and 110 mg/ml sodium pyruvate (GIBCO) containing 20% FBS (Wisent ...
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bioRxiv - Immunology 2024Quote: Single cell suspensions of mouse tissue and blood were stained using the LIVE/DEAD Fixable Blue cell stain (Thermofisher, L23105) for viability assessment and blocked with CD16/32 based FcBlock (Biolegend ...
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bioRxiv - Genetics 2024Quote: ... and 3.8 μL of water) to determine the additional number of PCR cycles needed for optimal cDNA amplification (Applied Biosystems QuantStudio 7 Flex). We then prepared PCR reactions per total number of barcoded beads collected for each sNucDrop-seq run ...
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bioRxiv - Genetics 2024Quote: ... and 110 mg/mL sodium pyruvate (GIBCO) containing 0.2% collagenase I (Sigma ...
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bioRxiv - Genetics 2024Quote: ... and 110 mg/ml sodium pyruvate (GIBCO) containing 20% FBS (Wisent ...
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bioRxiv - Genetics 2024Quote: ... Wild type N/TERT-2G cell line was cultured using EpiLife medium with 60 µM calcium (Gibco™, cat. #MEPI500CA), supplemented with human keratinocyte growth supplement (HKGS ...
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bioRxiv - Immunology 2024Quote: ... CD11c-efluor450 (Invitrogen, Clone: N41B), CD45.2-AF700 (Biolegend ...
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bioRxiv - Genetics 2024Quote: ... 2 ml of DMEM media (ref 31966047, Gibco, Life Technologies, USA) containing 10% of fetal bovine serum (Life Technologies ...
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bioRxiv - Genetics 2024Quote: ... Slides were covered by mounting medium with nuclear counterstain DAPI (Thermofisher, catalog number D1306, 5 ng/ml). Immunohistochemistry for zebrafish was performed as described20 ...
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bioRxiv - Genetics 2024Quote: ... COL6A2 (Thermofisher, catalog number PA5-65085, 1:200), COL4 (Thermofisher ...
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bioRxiv - Genetics 2024Quote: ... 2 ml of DMEM media (ref 31966047, Gibco, Life Technologies, USA) containing 10% of fetal bovine serum (Life Technologies ...
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bioRxiv - Genetics 2024Quote: ... Equal protein amounts were resolved by SDS-PAGE (Life Technologies, California, USA) and transferred to a nitrocellulose membrane (IBlot gel transfer ...
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bioRxiv - Genetics 2024Quote: ... Reverse transcription to cDNA was achieved by using RNA (1µg) and the Superscript vilo cDNA synthesis kit (#Cat. 11754050, Invitrogen, Massachusetts, USA). 25 ng of XPC and GAPDH (Qiagen ...
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bioRxiv - Genetics 2024Quote: ... and subjected to staining as per the guidelines provided by the manufacturer (Thermo Fisher Scientific, Massachusetts, USA). The analysis was carried out utilizing flow cytometry equipment (FACScan ...
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bioRxiv - Genomics 2024Quote: ... RNAs were extracted using the TRIzol Reagent (Thermo Fisher Scientific, Waltham, USA) according to the supplier’s recommendation and cleaned using the RNeasy Mini Kit (Qiagen) ...
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bioRxiv - Genomics 2024Quote: All animals in the study were genotyped using the Affymetrix Axiom™ Genome-Wide BOS-1 Array (Thermo Fisher Scientific) with SNP positions originally mapped to the UMD3.1 bovine reference genome assembly112 ...
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bioRxiv - Biochemistry 2024Quote: ... were diluted into 200 μl Opti-MEM (Gibco) in each well of a 6-well plate cells ...
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bioRxiv - Biochemistry 2024Quote: ... The absorbance at 340 nm was measured using a Varioskan LUX Multimode microplate reader (ThermoFisher). Kinetic parameters were generated by nonlinear regression analysis using Prism (GraphPad Software ...
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bioRxiv - Genomics 2024Quote: ... DNA concentration and DNA fragment length were assessed using the Qubit dsDNA BR Assay kit on the Qubit Fluorometer (Thermo Fisher Scientific) and the Genomic DNA Screen Tape on the Agilent 4150 TapeStation system (Agilent Technologies) ...
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bioRxiv - Biochemistry 2024Quote: ... Lentivirus was generated using HEK293FT cells (ATCC) and ViralPower lentiviral packaging mix (ThermoFisher Scientific), according to the manufacturer instructions ...
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bioRxiv - Biochemistry 2024Quote: ... dumerilii were amplified by PCR (Phusion® DNA polymerase, Thermo Fisher Scientific) with primers containing restriction enzyme sites for further cloning into pYES2 (Error! Reference source not found.) ...
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bioRxiv - Biochemistry 2024Quote: ... supplemented with 10% Heat Inactivated Fetal Bovine Serum (FBS; ThermoFisher Scientific) and 1% penicillin/streptomycin (ThermoFisher ...
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bioRxiv - Biochemistry 2024Quote: ... Austria) was homogenised in TRIzol ™ reagent for further extraction of total RNA according to the manufacturer’s instructions (Thermo Fisher Scientific, Waltham, MA, USA). Complementary DNA (cDNA ...
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bioRxiv - Biochemistry 2024Quote: ... dumerilii were cloned into the yeast expression vector pYES2 (Thermo Fisher Scientific) for further functional characterisation (below) ...
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bioRxiv - Biochemistry 2024Quote: ... and 1% penicillin/streptomycin (ThermoFisher. Scientific) in a 37°C tissue culture incubator with 5% CO2 ...
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bioRxiv - Biochemistry 2024Quote: ... Coverslips were mounted on glass slides with Prolong™ Gold antifade reagent (Thermofisher Scientific), allowed to cure overnight ...
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bioRxiv - Biochemistry 2024Quote: ... was separated by SDS-PAGE (NuPage 4-12% Bis-Tris, Invitrogen) and blotted onto a nitrocellulose membrane ...
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bioRxiv - Biochemistry 2024Quote: Fluorescent labelling of Tau and 14-3-3ζ protein for microscopy assays was done using amine-reactive DyLight405 or DyLight488-NHS ester (Thermo Scientific) following the manufacturer instructions and the established protocol 34.