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Citations for Thermo Fisher :
5351 - 5400 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... and equal volume for all SEC fractions were electrophoresed on 4-12% NuPAGE™ Bis-Tris in NuPAGE™ MES SDS Running Buffer (Thermo Scientific) for 60 min at 160 V ...
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bioRxiv - Immunology 2024Quote: ... SuperScript™ III One-Step RT-PCR (ThermoFisher Scientific, Waltham, MA) was used according to the manufacturer’s instruction to amplify Mu Mx1 RNA using Mu Mx1 specific primers ...
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bioRxiv - Immunology 2024Quote: ... Pierce protein G IgG binding buffer (Thermo Fisher Scientific) was used to dilute the serum 1:1 before loading as well as for equilibration and wash steps ...
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bioRxiv - Immunology 2024Quote: Cells were seeded into an I-Bidi 8-well chambered slide (Fisher Scientific, Waltham, MA). At 60-70% confluency ...
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bioRxiv - Immunology 2024Quote: ... CyRPA protein were expressed as secreted protein by transient transfection of HEK Expi293 cells (Thermo Fisher Scientific) following the manufacturer’s protocol using ExpiFectamine™ (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2024Quote: ... All supernatants were harvested via centrifugation and the proteins purified using CaptureSelect C-tag affinity matrix (Thermo Fisher Scientific) on an ÄKTA Pure FPLC system (Cytiva) ...
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bioRxiv - Immunology 2024Quote: ... and purified via CaptureSelect™ C-tag affinity matrix (Thermo Fisher Scientific). A further SEC polishing step was performed on a HiLoad 16/600 Superdex 200 pg column (GE Healthcare ...
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bioRxiv - Immunology 2024Quote: RNA extractions were performed using Ambion Magmax kit (ThermoFisher Scientific, Waltham, MA) on a KingFisher Flex system (ThermoFisher Scientific ...
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bioRxiv - Biochemistry 2024Quote: ... Cryo-EM data were collected with a 300 kV Krios G4 transmission electron microscope (Thermo Fisher Scientific, USA) equipped with a BioQuantum energy filter and K3 camera (Gatan ...
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bioRxiv - Biochemistry 2024Quote: ... and stained with Simply Blue (Thermo Fisher Scientific).
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bioRxiv - Biochemistry 2024Quote: ... All images were automatically recorded using the EPU software (Thermo Fisher Scientific, USA). For the kpRat1(2A)/Rai1(2A)/Rtt103 complex ...
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bioRxiv - Biochemistry 2024Quote: ... Protein was expressed using ExpiFectamine 293 Transfection Kit (Thermo Fisher Scientific). Expi293F cells were grown at 37℃ with 8% CO2 to 3E6 cells and transfected with 100µg of DNA ...
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bioRxiv - Biochemistry 2024Quote: ... Protein was expressed using ExpiFectamine 293 Transfection Kit (Thermo Fisher Scientific). Expi293F cells were grown at 37℃ with 8% CO2 to 3E6 cells and transfected with 100 µg of DNA ...
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bioRxiv - Biochemistry 2024Quote: ... Cells were transfected with 24 μg of DNA and 60 μL of Lipofectamine 2000 (Thermo Fisher Scientific) in Opti-MEM transfection medium ...
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bioRxiv - Biochemistry 2024Quote: ... Protein was expressed using ExpiFectamine 293 Transfection Kit (Thermo Fisher Scientific). Expi293F cells were grown at 37℃ with 8% CO2 to 3E6 cells and transfected with 100 µg of DNA ...
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bioRxiv - Biochemistry 2024Quote: ... Peptides were separated on an ES902 Easy-Spray column (Thermo Scientific). The composition of mobile phases A and B was 0.1% formic acid in HPLC water ...
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bioRxiv - Biochemistry 2024Quote: ... Data acquisition was performed on a system where an Ultimate 3000 HPLC (Thermo Scientific) was coupled to an Orbitrap Lumos mass spectrometer (Thermo Scientific ...
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bioRxiv - Biochemistry 2024Quote: ... was coupled to an Orbitrap Lumos mass spectrometer (Thermo Scientific) via an Easy-Spray ion source (Thermo Scientific) ...
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bioRxiv - Biochemistry 2024Quote: ... via an Easy-Spray ion source (Thermo Scientific). For each LC-MS/MS run ...
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bioRxiv - Neuroscience 2024Quote: ... and detected using secondary antibody donkey anti-goat Alexa Fluor® 647 (Thermo Fisher Scientific, #A21447) was diluted in blocking solution (1% BSA in PBS ...
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bioRxiv - Neuroscience 2024Quote: ... donkey anti-mouse AlexaFluor-488 (Invitrogen), was diluted 1:1000 in donkey serum (2% ...
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bioRxiv - Neuroscience 2024Quote: ... Alexa Fluor 488 goat anti-rabbit antibody (1:2000, A32731, Invitrogen, Thermo Fisher Scientific) and Alexa Fluor 633 goat anti-mouse antibody (1:2000 ...
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bioRxiv - Neuroscience 2024Quote: ... and Alexa Fluor 633 goat anti-mouse antibody (1:2000, A21050, Life Technologies) in PBS ...
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bioRxiv - Neuroscience 2024Quote: ... and rabbit anti-MAP2 (PA5-17646, Invitrogen, Thermo Fisher Scientific) in a 1:2000 solution ...
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bioRxiv - Neuroscience 2024Quote: ... in NeurobasalTM Plus (A3582901, Gibco) was used as a secondary coating by adding the same volumes to the substrates and incubating at 37 °C overnight ...
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bioRxiv - Neuroscience 2024Quote: ... Alexa Fluor 488 goat anti-rabbit antibody (1:2000, A32731, Invitrogen, Thermo Fisher Scientific) and Alexa Fluor 633 goat anti-mouse antibody (1:2000 ...
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bioRxiv - Neuroscience 2024Quote: ... and rinsed with 2 ml NeurobasalTM Plus (A3582901, Gibco) preheated to 37 ◦C ...
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bioRxiv - Neuroscience 2024Quote: ... respectively in phosphate buffered saline (PBS) (10010015, Gibco, Thermo Fisher Scientific) for 45 min at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... Tissues were counterstained with NucBlue Fixed Cell ReadyProbes Reagent (DAPI) (ThermoFisher Scientific, catalog #R37606) included in the secondary antibody solution ...
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bioRxiv - Neuroscience 2024Quote: ... respectively in phosphate buffered saline (PBS) (10010015, Gibco, Thermo Fisher Scientific) for 45 min at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... neurons were washed and mounted in ProLong Gold Antifade Mountant (Thermo Fisher Scientific, #P36934). Confocal images were acquired with a spinning-disk confocal system consisting of an Axio Observer Z1 equipped with a CSU-W1 spinning-disk head ...
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bioRxiv - Neuroscience 2024Quote: ... and finally detected by chemiluminescence after incubating with HRP substrate (Thermo Scientific™, #P32106). For mouse brain proteins ...
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bioRxiv - Neuroscience 2024Quote: ... with the MAGnify Chromatin Immunoprecipitation System (ThermoFisher Scientific, Cat. 492024) following the standard protocol ...
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bioRxiv - Neuroscience 2024Quote: ... The protein concentrations in the cytoplasmic and nuclear fractions were determined using a Pierce BCA Protein Assay Kit (ThermoFisher Scientific, Cat. 23228). The isolated cytoplasmic and nuclear proteins were aliquoted and stored at -80°C for subsequent analysis.
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bioRxiv - Neuroscience 2024Quote: ... 2 mM L-glutamine (Gibco, Cat. 25030149), 10% FBS ...
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bioRxiv - Neuroscience 2024Quote: Mouse DRG neurons were seeded on a glass-bottom cell culture dish and cultured in neurobasal medium (Gibco, Cat. 21103049) supplemented with B-27 supplement (Gibco ...
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bioRxiv - Molecular Biology 2024Quote: The membrane was incubated with 10 mL of ULTRAhyb Ultrasensitive Hybridization Buffer (Thermo Fisher) at 42°C for 1 h ...
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bioRxiv - Molecular Biology 2024Quote: Transfection reagent Lipofectamine™ 3000 transfection reagent (Invitrogen, USA) was utilized to promote siRNA get into cells ...
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bioRxiv - Molecular Biology 2024Quote: ... cultured in DMEM (Gibco, USA) containing 10% fetal bovine serum (FBS ...
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bioRxiv - Neuroscience 2024Quote: ... samples were acquired using a Luminex FlexMap3D system (Thermo Fisher) and analyzed using the BioPlex Software from BioRad.
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bioRxiv - Neuroscience 2024Quote: ... and granulocyte-macrophage colony-stimulating factor (GM-CSF, 10 ng/ml) to DMEM/F12 (HyClone™, SH30023.01) containing N-2 supplement (Gibco™, 17502048). This protocol facilitated the maturation process of the PMPs into microglia.
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bioRxiv - Neuroscience 2024Quote: ... the cultures were incubated overnight at 4°C with the following primary antibodies which were diluted in blocking buffer: rabbit anti-CD235 (Invitrogen, 90757), mouse anti-CD43 (Invitrogen ...
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bioRxiv - Neuroscience 2024Quote: ... a complete neurobasal medium (Invitrogen, Cat. 21103049) with 2% horse serum was added to the tubes and centrifuged for 2 min at 800× g ...
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bioRxiv - Neuroscience 2024Quote: ... and 1% antibiotic-antimycotic (Gibco, Cat. 15240062). Human iPSC-derived sensory neurons were cultured in dishes coated with Matrigel matrix (Corning ...
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bioRxiv - Neuroscience 2024Quote: PDAC cells were homogenized using TRIzol reagent (ThermoFisher Scientific, #15596018) to extract RNA ...
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bioRxiv - Neuroscience 2024Quote: ... Protein concentration was determined by the standard Pierce™ BCA Protein Assay Kit (Thermo Scientific™, #23225).
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bioRxiv - Neuroscience 2024Quote: ... and chromatographic separation was performed using the Easy-nLC1200 chromatographic system (ThermoFisher Scientific). Subsequently ...
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bioRxiv - Neuroscience 2024Quote: ... Tau pS202/T205 (AT8, Thermo Scientific™, #MN1020), Tau pS214 (abcam ...
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bioRxiv - Neuroscience 2024Quote: ... the tissue sections were incubated with species-specific secondary antibodies (ThermoFisher Scientific, USA) for 45 min at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... Protein concentration was detected by the Pierce™ BCA Protein Assay Kit (Thermo Scientific™, #23225). Whole protein lysate was thus extracted and used for the subsequent western blots.