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Citations for Thermo Fisher :
5001 - 5050 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... supplemented with 1% AlbuMax (Invitrogen, Milan, Italy), 2 mM L-glutamine (Euroclone) ...
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bioRxiv - Microbiology 2024Quote: ... in-house immobilized onto POROS-20AL perfusion resin (Thermo Scientific) as described previously 69 ...
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bioRxiv - Microbiology 2024Quote: ... A mixed asexual culture of 1% was set up and cultured in medium supplemented with 2.5% Albumax II (Thermo Fisher Scientific™) without human serum for four days ...
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bioRxiv - Neuroscience 2024Quote: ... RNA was quantified using NanoDrop TM 2000 (Thermo Scientific, Rhenium, Modi’in, Israel). Two micrograms of total RNA were reverse transcribed using High-Capacity RNA to cDNA kit (Applied Biosystems ...
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bioRxiv - Microbiology 2024Quote: ... We used the Pierce BCA Protein Assay Kit (Thermo Fisher) to measure the protein concentration from all three elution fractions ...
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bioRxiv - Molecular Biology 2024Quote: ... and incubated in the following primary antibodies overnight at 4°C: rabbit-anti-ZO1 (40-2200, Thermo Fisher Scientific, 1:200) and mouse-anti-RPE65 (MAB5428 ...
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bioRxiv - Microbiology 2024Quote: ... To transfer the binding reactions to the nylon membrane (Biodyne™ B Nylon Membrane, Thermo Scientific), we semi-dry transferred the reactions at 15 V for 45 min and immediately crosslinked the transferred DNA to the membrane using a commercial UV-light crosslinking oven (UV Stratalinker ...
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bioRxiv - Molecular Biology 2024Quote: ... Reverse transcription and PCR were performed on 35ng of total RNA per well using AgPath-ID One-Step RT-PCR Reagents (4387424, Thermo Fisher Scientific) with the following TaqMan primer-probe sets (all Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2024Quote: ... Platinum™ Taq polymerase (Invitrogen™, USA); Q5U® Hot Start High-Fidelity DNA Polymerase (New England Biolabs ...
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bioRxiv - Molecular Biology 2024Quote: ... with the following TaqMan primer-probe sets (all Thermo Fisher Scientific): EIF2B2 (Hs00204540_m1) ...
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bioRxiv - Molecular Biology 2024Quote: ... RPE cells were enzymatically passaged using TrypLE Select (Gibco #12563011) per manufacturer’s instructions and seeded onto tissue culture plates coated with Matrigel hESC-Qualified Matrix (Corning #354277 ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein concentrations of the lysed sEV preparations were quantified using the Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific, USA), following the manufacturer’s protocols (Leung et al. ...
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bioRxiv - Microbiology 2024Quote: ... We used the LightShift Chemiluminescent EMSA Kit (Thermo Scientific) and protocol for all EMSA reactions ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 L of untreated sewage was collected and processed (150 mL) using ammonium sulphate precipitation and nucleic acid extraction using a Kingfisher Flex™ (Thermo Scientific™, UK) and NucliSENS® (BioMérieux ...
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bioRxiv - Microbiology 2024Quote: ... Cells were immobilised on 1% agarose pads prepared with 1.0 × 1.0-cm GeneFrames (Thermo Fisher Scientific). Unless stated otherwise ...
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bioRxiv - Microbiology 2024Quote: ... then the membrane was prehybridized for 45 min in ULTRAhyb (Ambion) solution at 42 °C ...
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bioRxiv - Microbiology 2024Quote: ... Proteins were transferred onto a polyvinylidene difluoride (PDVF) membrane using the iBlot2 Dry Blotting System (ThermoFisher Scientific). The membrane was blocked in 5% non- fat milk or bovine serum albumin (BSA ...
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bioRxiv - Microbiology 2024Quote: ... DMEM containing glucose was either purchased (Gibco) or prepared in a similar manner with the addition of 25 mM glucose ...
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bioRxiv - Microbiology 2024Quote: ... and GFP+ single-cells were sorted into 96-well plates (ThermoFisher Scientific). Cells were allowed to expand for over 3 weeks into clonal populations ...
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bioRxiv - Microbiology 2024Quote: ... Ribose (100 mM; Millipore or ThermoFisher Scientific), Uridine (100 mM ...
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bioRxiv - Microbiology 2024Quote: ... NCBI ID: JX869059) with removed potential splice sites were cloned into a pcDNA3.1 expression vector (V79020, ThermoFisher) in frame with a C-terminal V5-tag ...
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bioRxiv - Microbiology 2024Quote: ... and an APC-conjugated CD4 antibody (ThermoFisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were rinsed with DPBS (Gibco #14040141) and fed with 4mL XVIVO-10 medium (Lonza #(BE)BP04-743Q ...
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Molecular basis of proteolytic cleavage regulation by the extracellular matrix receptor dystroglycanbioRxiv - Biochemistry 2024Quote: ... and 1:500 dilution Alexa Flour 488 conjugated to anti-HA (Invitrogen) and rotated at 4°C ...
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bioRxiv - Molecular Biology 2024Quote: ... 2 x 106 cells were cultured in 10 mL high glucose Dulbecco’s Modified Eagle’s Medium (Thermo Fisher Scientific, Waltham, MA) containing sodium pyruvate (110 mg/L) ...
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bioRxiv - Molecular Biology 2024Quote: ... The diluted pre-amplified products were then utilized directly for real-time PCR reactions according to the manufacturer’s protocol (Applied Biosystems™ TaqMan™ Array Human MicroRNA A+B Cards Set v3.0, ThermoFisher Scientific). For the data analysis ...
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bioRxiv - Molecular Biology 2024Quote: ... The diluted pre-amplified products were then utilized directly for real-time PCR reactions according to the manufacturer’s protocol (Applied Biosystems™ TaqMan™ Array Human MicroRNA A+B Cards Set v3.0 ...
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bioRxiv - Molecular Biology 2024Quote: ... The purity and quantity of the isolated total RNA were measured using a Nanodrop™ 2000 spectrophotometer (Thermo Fisher Scientific, Wilmington, DE).
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bioRxiv - Microbiology 2024Quote: ... 50 μg/ml kanamycin (Fisher Scientific), 10 μg/ml tetracycline (GoldBio) ...
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bioRxiv - Microbiology 2024Quote: ... The membrane was placed into a hybridization bottle and was prehybridized with 5 ml ULTRAhyb Ultrasensitive Hybridization Buffer (Invitrogen) at 42°C for 45 minutes in a hybridization oven ...
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bioRxiv - Microbiology 2024Quote: ... A BrightStar-Plus Positively Charged Nylon Membrane (Invitrogen) was cut to size and soaked in RNase-free water before incubation in 10X sodium saline citrate (SSC ...
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bioRxiv - Microbiology 2024Quote: ... using the KinaseMax Kit (Invitrogen) according to the manufacturer’s suggestions ...
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bioRxiv - Microbiology 2024Quote: ... as pre-mixed powders or were made with individual medium components purchased from Fisher Scientific. Bacteria were cultured in LB broth or on LB agar plates at 37°C unless otherwise stated ...
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bioRxiv - Microbiology 2024Quote: ... mixed with 500 µL of TRIzol Max reagent (Invitrogen, Carlsbad, CA, USA) and homogenized with 0.8 g of sterilized 0.1 mm zirconia beads for three min (3 × 1 min with cooling in between ...
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bioRxiv - Microbiology 2024Quote: ... An equivalent volume (180 μl) of acid phenol:chloroform (pH 4.5) (Ambion) was added and each sample was transferred to a 2.0-ml Phase Lock Gel Heavy wax tube ...
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bioRxiv - Microbiology 2024Quote: ... Samples which contained over 10 ng/µL DNA contamination were treated with the Turbo DNAfree® Kit (Ambion, Bleiswijk, Netherlands) following manufacturer’s instructions and purified using the RNeasy Mini Kit ...
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bioRxiv - Microbiology 2024Quote: ... in a 2.0-ml RNase-free Microcentrifuge Tube (Invitrogen) at 65°C ...
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bioRxiv - Neuroscience 2024Quote: ... before covering with Shandon-mount (Thermo scientific, Rhenium, Modi’in, Israel, #1900331). Immunohistochemistry images were taken with Zeiss AxioImager M2 with the apotome system or Axio Scan.Z1 microscope (Zeiss ...
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bioRxiv - Neuroscience 2024Quote: ... Immulon-2 plates (Thermo Scientific) were incubated with 500 ng streptavidin (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... Two micrograms of total RNA were reverse transcribed using High-Capacity RNA to cDNA kit (Applied Biosystems, Rhenium, Modi’in, Israel, #4368814). Quantitative PCR analysis was performed using or FAST SYBR Green master mix (Applied Biosystems ...
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bioRxiv - Neuroscience 2024Quote: ... or Goat αMouse Cy-3 (Invitrogen, #A10521) (1:500) ...
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bioRxiv - Neuroscience 2024Quote: ... Quantification of the library was performed using dsDNA HS Assay Kit and Qubit 2.0 (Molecular Probes, Life Technologies) and qualification was done using the Agilent D1000 Tapestation Kit and Tapestation 4200 ...
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bioRxiv - Molecular Biology 2024Quote: ... Signal detection was performed using Pierce ECL substrate reagent (Thermo Fisher Scientific, Cat# 32106) on a ChemiDoc machine (Bio-Rad) ...
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bioRxiv - Biochemistry 2024Quote: ... in 10 mM Tris-HCl-HCl (15567027, Invitrogen™), freshly prepared ...
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bioRxiv - Neuroscience 2024Quote: ... Incubation for one hour at room temperature was made with secondary antibody: Goat αRabbit Alexa flour 488 (Invitrogen Rhenium, Modi’in, Israel, #A-11008) (1:200 ...
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bioRxiv - Molecular Biology 2024Quote: ... membranes were stripped with Restore™ stripping buffer (Thermo Fisher Scientific, Cat# 21059) and reprobed with Anti-beta actin antibody.
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bioRxiv - Neuroscience 2024Quote: ... for 10 minutes and then mounted on slides (Thermo scientific, Rhenium ...
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bioRxiv - Neuroscience 2024Quote: ... Quantitative PCR analysis was performed using or FAST SYBR Green master mix (Applied Biosystems, Rhenium ...
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bioRxiv - Microbiology 2024Quote: ... Real time PCR assay was performed on Quant studio 5 real time PCR machine (Thermo Fisher Scientific, USA). Sample generation for PCR validation was done independent of that for transcriptome assay.
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bioRxiv - Molecular Biology 2024Quote: ... The worm pellets were resuspended in 800 µL TRIzol (Thermo Fisher Scientific, Waltham, MA United States). Worms were broken up prior to RNA extraction by treating the samples with four rounds of freeze-thaw cycles using liquid nitrogen and a thermo block at 46 °C ...