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Citations for Thermo Fisher :
5051 - 5100 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... extracted RNA was quantified using a Qubit 4.0 fluorometer (Thermofisher; Q33238). RNA concentration and purity were evaluated using Nanodrop 1000 ...
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bioRxiv - Microbiology 2024Quote: Keratinocyte serum-free media (K-SFM; Life Technologies, Thermo Fisher Scientific) was used to cultivate N/TERT-1 cells ...
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bioRxiv - Molecular Biology 2024Quote: ... and 1X GlutaMAX (ThermoFisher). Mouse primary hepatocytes (Cell Biologics C57-6224F ...
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bioRxiv - Molecular Biology 2024Quote: ... Organic phases were combined and evaporated to dryness using a SpeedVac Savant centrifugal evaporator (Thermo Scientific, Dreieich, Germany). Proteins were extracted from the residue debris pellets and quantified using a BCA kit (Sigma-Aldrich ...
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bioRxiv - Microbiology 2024Quote: Keratinocyte serum-free media (K-SFM; Life Technologies, Thermo Fisher Scientific) was used to cultivate N/TERT-1 cells ...
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bioRxiv - Biochemistry 2024Quote: ... Plates were washed again and incubated with 50 µL of ODP (ThermoFisher 34006), prepared according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: Human THP-1 monocytes (ATCC catalog no. TIB-22) were cultured in RPMI medium with 10% FBS (Seradigm) and 1% penicillin-streptomycin (Gibco) at 37°C and 5% CO2 ...
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bioRxiv - Biochemistry 2024Quote: ... Coverslips were mounted onto microscope slides with Prolong Gold antifade (ThermoFisher, P10144). Images were captured on a Zeiss LSM 980 with Airyscan2 using a 63× oil objective and processed with Zeiss Blue Airyscan joint deconvolution.
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bioRxiv - Biochemistry 2024Quote: ... which was then converted to cDNA using Superscript III (Invitrogen 18080051). VHH genes were amplified using gene specific primers for long- and shot-hinge heavy-chain-only antibodies (Maass et al. ...
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bioRxiv - Biochemistry 2024Quote: ... MaxiSorp plates (Invitrogen 442404) were coated with ESAT-6 at 5 µg/mL in PBS overnight at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... and the Quant-iT RNA assay kit (Invitrogen, Carlsbad, CA). TruSeq libraries were prepared using the RNA Sample Prep Kit v2 and sequenced on an Illumina HiSeq 2000 (Illumina) ...
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bioRxiv - Neuroscience 2024Quote: ... The Sarkosyl-soluble and insoluble fractions were loaded separately onto NuPAGE™ 4-12% Bis-Tris Polyacrylamide Gels (Thermo Fisher Scientific) and immunoblotted as described in the Western Blotting section ...
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bioRxiv - Neuroscience 2024Quote: ... The Sarkosyl-soluble supernatant was then collected and resuspended in 1× NuPAGE™ LDS Sample Buffer (Thermo Fisher Scientific). The Sarkosyl-insoluble pellet was washed several times in 1% Sarkosyl solution prepared in cold buffer H and then resuspended in 1x NuPAGE™ LDS Sample Buffer ...
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bioRxiv - Microbiology 2024Quote: ... propidium iodide (PI) (Invitrogen, Eugene, Oregon, USA) served as the fluorescent reporter dye ...
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bioRxiv - Molecular Biology 2024Quote: ... LC-MS measurements were performed on a Dionex Ultimate 3000 (Thermo Fisher Scientific) coupled to an Impact II qToF mass spectrometer (Bruker Daltonics) ...
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bioRxiv - Biochemistry 2024Quote: ... and ExpiCHO feed (ThermoFisher Scientific #A29129) was added to the flask ...
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bioRxiv - Molecular Biology 2024Quote: ... a semi-quantitative comparison of lipid abundance and annotated peaks were integrated using Compound Discoverer 3.3 (Thermo Scientific). The data were normalized to the maximum peak area sum of all samples ...
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bioRxiv - Microbiology 2024Quote: ... directed against the cytoplasmic domain of E-cad and a monoclonal anti-Occludin mouse mAb (331500, Life Technologies SA) and revealed using an anti-mouse IgG (H+L ...
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bioRxiv - Molecular Biology 2024Quote: 1-3*106 NSC34 cells were lysed in buffer R-Lysis (25 mM HEPES pH 7.5, 100 mM NaCl, 1X protease inhibitor (ThermoFIsher, 78429)) and subjected to a water-bath sonication (35-40 amplitude ...
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bioRxiv - Microbiology 2024Quote: ... and revealed using an anti-rabbit IgG (H+L) secondary antibody (Alexa Fluor 647) (Life Technologies,France). 4’,6’-diamino-2-fenil-indol (DAPI ...
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bioRxiv - Molecular Biology 2024Quote: ... Stained cells were washed with DPBS and maintained in FluoroBrite DMEM (# A1896701, Gibco) supplemented with 1% P/S to preserve cell fitness throughout the imaging process ...
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bioRxiv - Molecular Biology 2024Quote: ... MitoSOXTM Red mitochondrial superoxide indicator (invitrogen by Thermo Fisher Scientific, Cat#1842725) and APC anti-mouse Ly6C antibody (Biolegend ...
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bioRxiv - Microbiology 2024Quote: ... 5’ probes FAM-ACCCCGCATTACGTTTGGTGGACC-QSY 3’) and Quantitative Superscript III Platinum One-step RT-qPCR systems with the ROX kit (Invitrogen), according to the manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2024Quote: ... 1:200 cDNA was mixed with 1X hsa-miR-221-3p Advanced miRNA Assay (477981_mir, ThermoFisher-A25576) and ddPCR Supermix for Probes (Bio-Rad - 1863026 ...
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bioRxiv - Molecular Biology 2024Quote: ... miRNA TaqMan probes were bought from ThermoFisher with the following codes ...
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bioRxiv - Microbiology 2024Quote: ... were cultured in Dulbecco’s modified Eagle medium with high glucose content (DMEM-F12, Gibco, Waltham, MA, USA) supplemented with 10% foetal bovine serum (FBS ...
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bioRxiv - Molecular Biology 2024Quote: ... 1X protease inhibitor (ThermoFisher-78429). Cell lysates were loaded on 10% acrylamide gels and transferred to a PVDF membrane ...
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bioRxiv - Microbiology 2024Quote: ... and revealed using an anti-mouse IgG (H+L) secondary antibody (Alexa Fluor 555) (Life Technologies). For the SARS-CoV-2 staining a polyclonal rabbit anti-SARS Coronavirus Spike protein (PA1-41142 ...
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bioRxiv - Microbiology 2024Quote: ... containing 4% FCS and 1% L-Glutamine (L-Gln; Invitrogen). These adherent cells were cultured to confluence for five days at 37 °C in an atmosphere containing 5% CO2.
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bioRxiv - Molecular Biology 2024Quote: ... and 1% penicillin/streptomycin (Life Technologies, Carlsbad, CA, USA) in standard conditions at 37°C with 5% CO2.
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bioRxiv - Molecular Biology 2024Quote: ... the supernatant was collected and 200 µg were incubated with 5 μl of buffer equilibrated DynabeadsTM M-280 Streptavidin (Invitrogen, 11205D) for 15 min at 4 °C in rotation to perform a pre-clearing step ...
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bioRxiv - Microbiology 2024Quote: ... followed by incubation with an anti-E-cadherin mouse mAb (4A2C7, Life Technologies, France) directed against the cytoplasmic domain of E-cad and a monoclonal anti-Occludin mouse mAb (331500 ...
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bioRxiv - Molecular Biology 2024Quote: The following primary antibodies were used: anti-hnRNPA2B1 (PA5-34939, Invitrogen), anti-Calnexin (Abcam) ...
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bioRxiv - Microbiology 2024Quote: ... The absorption at 486 nm (A486) and 390 nm (A390) was measured at specific time points using the Varioskan Flash (Thermo Scientific). The specific β-lactamase activity was calculated as ratio between A486/A390 (sample ...
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bioRxiv - Molecular Biology 2024Quote: ... cDNA was produced using TaqMan™ Advanced miRNA cDNA Synthesis Kit (Applied Biosystems™-A28007) following manufacturers’ instructions and used for droplet digital PCR experiments.
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bioRxiv - Microbiology 2024Quote: ... The culture was mixed 1:1 with the BacTiter-Glo substrate and luminescence was measured using the Varioskan Flash (Thermo Scientific). Cell viability was determined in duplicates by calculating the ratio of luminescence of the sample and medium.
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bioRxiv - Microbiology 2024Quote: ... was cultured in Minimum Essential Medium (MEM, Gibco; Invitrogen) containing 4% FCS and 1% L-Glutamine (L-Gln ...
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bioRxiv - Cell Biology 2024Quote: ... either 3.6 µg of scramble shRNA or MAP6 shRNA were transfected into hippocampal neurons using Lipofectamine 2000 transfection reagent (Invitrogen, 11668030). Neurons were then collected for protein analysis using western blot.
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bioRxiv - Cell Biology 2024Quote: ... Dynabeads MyOne Streptavidin C1 beads were purchased from ThermoFisher. N2-4-Ethynylbenzyl-2’-deoxyguanosine (EBndG ...
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bioRxiv - Cell Biology 2024Quote: ... 2% B27 supplement (Gibco, A3582801), 0.297% Glucose (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2024Quote: ... cells were washed twice with DPBS and lysed using RIPA buffer containing phosphatase and protease inhibitors (Thermo Fisher Scientific: A32957; A32953). Whole cell lysate was passed through a 27G needle (BD Biosciences ...
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bioRxiv - Cell Biology 2024Quote: ... Then an aliquot of the cell suspension was taken and stained with an equal volume of Trypan Blue (Gibco: 15250-061), and live/dead cell number was assessed using a Countess II Automated Cell Counter (Thermo Fisher) ...
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bioRxiv - Cell Biology 2024Quote: ... puromycin 100μM (Gibco, cat#A1113803), a protein synthesis inhibitor that maintains the SEC61 translocon in a calcium permeable state25 ...
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bioRxiv - Cell Biology 2024Quote: ... Protein samples were heat denatured and separated on 4–12% Bolt Bis-Tris gels (Thermo Fisher Scientific: NW04125BOX). The protein was then transferred onto a 0.2μM nitrocellulose membrane (VWR ...
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bioRxiv - Cell Biology 2024Quote: Knockouts were verified by Sanger sequencing (Genewiz) of the PCR product after column purification (Thermo Fisher Scientific: K0701).
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bioRxiv - Cancer Biology 2024Quote: ... Real-time quantitative PCR reactions were performed on a 7300 Real-time PCR system (Applied Biosystems) using the THUNDERBIRD SYBR qPCR Mix (Toyobo ...
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bioRxiv - Cell Biology 2024Quote: ... cells were washed twice with DPBS and then lysed with RNA STAT 60 (Fisher Scientific: NC9884083). Chloroform (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2024Quote: The hippocampus was dissected from TP18-day Sprague Dawley rat fetuses of either sex using cold dissection medium L-15 (Gibco, 21083027). Subsequently ...
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bioRxiv - Cell Biology 2024Quote: ... cells were washed twice with DPBS and metabolites were extracted with -80°C LC-MS grade 80:20 MeOH:H2O (Fisher Scientific: A456-4; W64). The extract was transferred to a tube ...
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bioRxiv - Cell Biology 2024Quote: ... consisting of neurobasal medium (Gibco, 21103049), 2% B27 supplement (Gibco ...