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Citations for Thermo Fisher :
4951 - 5000 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... the nucleus pellet was thoroughly resuspended in TRIzol (Invitrogen) by vigorous vortexing and subsequently mixed with chloroform ...
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bioRxiv - Molecular Biology 2024Quote: RNAs (10-50 μM) with a 5’ terminal hydroxyl group were incubated with T4 polynucleotide kinase (Thermo Scientific) and 𝛾-32P-ATP (10 mCi/ml ...
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bioRxiv - Molecular Biology 2024Quote: ... HeLa Kyoto (passage 15-35) were grown in 4.5g/L glucose DMEM (Life Technologies, 41965-039) supplied with 10 % fetal bovine serum (Life Technologies ...
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bioRxiv - Molecular Biology 2024Quote: Cells were first reverse transfected with siRNAs at a final concentration of 10 nM using Lipofectamine RNAiMAX (Invitrogen) and seeded in 96-well glass-botom imaging plates ...
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bioRxiv - Molecular Biology 2024Quote: ... and 1X Halt protease inhibitor (Thermo Fisher) were added ...
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bioRxiv - Molecular Biology 2024Quote: ... and the RNA concentration was measured using a Qubit Fluorometer with the Qubit RNA High Sensitivity Assay Kit (Invitrogen). The quality of the RNA was assessed using a high sensitivity electrophoresis system ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.3 µL of Lipofectamine 2000 (Life Technologies, 11668030) in 20 µL of DMEM was then added to the DNA premix and incubated for 20 min at RT before being added to the wells ...
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bioRxiv - Molecular Biology 2024Quote: ... Hoechst 33342 (Thermo Fisher) was added to the tissue homogenates at a concentration of 1 µg/ml to label nuclei ...
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bioRxiv - Molecular Biology 2024Quote: ... and one guide sequence (Table 1) was generated by following the MEGAscript®Kit (Invitrogen) protocol ...
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MALAT1 mediates miR-206/CDC42/PAK1/Paxillin signaling axis to alleviate erectile dysfunction in ratsbioRxiv - Molecular Biology 2024Quote: ... the BM-MSCs was transfected using Lipofectamine® 3000 (Invitrogen, USA) before chemical treatment.
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bioRxiv - Molecular Biology 2024Quote: ... the tissues were additionally incubated with BODIPY 493/503 (Invitrogen) for 30 min ...
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bioRxiv - Molecular Biology 2024Quote: ... Imaging was done the following day by replacing the medium with pre-warmed imaging medium (CO2-independent Leibovitz’s-15 medium (Gibco) containing 5% fetal bovine serum (Sigma-Aldrich ...
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bioRxiv - Molecular Biology 2024Quote: ... and the extracted RNA was then converted into cDNA using the High-Capacity cDNA Reverse Transcription Kit from Applied Biosystems. qRT-PCR was conducted using the SYBR Green PCR Master Mix on a QuantStudio5 system ...
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bioRxiv - Molecular Biology 2024Quote: ... After 20 min cells were washed with imaging medium (Invitrogen, A14291DJ) four times before imaging.
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bioRxiv - Molecular Biology 2024Quote: ... sample analysis was conducted on an UltiMate 3000 UHPLC system (ThermoFisher scientific) with absorbance monitoring at 260 nm ...
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bioRxiv - Microbiology 2024Quote: ... RNAs from the gel were transferred to a BrightStar-Plus Positively Charged Nylon Membrane (Invitrogen) using an electrotransfer apparatus (Bio-Rad) ...
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bioRxiv - Microbiology 2024Quote: ... and residual DNA concentrations were measured with the Qubit® dsDNA BR Assay Kit (Life Technologies, Leusden, the Netherlands). Samples which contained over 10 ng/µL DNA contamination were treated with the Turbo DNAfree® Kit (Ambion ...
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bioRxiv - Microbiology 2024Quote: ... The membrane was placed into a hybridization bottle and was prehybridized with 10 ml ULTRAhyb Ultrasensitive Hybridization Buffer (Invitrogen) at 42°C for 45 minutes in a hybridization oven ...
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bioRxiv - Microbiology 2024Quote: ... 1 ml of bacterial cell culture was mixed with a lysis solution containing SDS, EDTA, sodium acetate, and UltraPure Phenol:Water (3.75:1, v/v) (Invitrogen) in a 2.0-ml RNase-free Microcentrifuge Tube (Invitrogen ...
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bioRxiv - Microbiology 2024Quote: RNA extracts were treated with TURBO DNase (Invitrogen) to remove contaminating DNA as described above ...
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bioRxiv - Microbiology 2024Quote: ... RNA samples were subjected to DNase digestion using TURBO DNase (Invitrogen) according to the manufacturer’s recommendations except twice the recommended enzyme units (4 U ...
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bioRxiv - Microbiology 2024Quote: ... CountBright beads (Molecular Probes) were added to each sample to determine measured volume ...
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bioRxiv - Microbiology 2024Quote: ... samples were stained with SYBRGreen I (Molecular Probes, Eugene, OR, USA) at a 10-4 (v/v ...
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bioRxiv - Microbiology 2024Quote: ... consisting of conjugated antibodies (Affymetrix, Santa Clara, California). The raw data obtained from the assay were normalized against the median fluorescence intensity (MFI ...
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bioRxiv - Microbiology 2024Quote: ... the cells pellets were fixed in 50 µl of 0.5% paraformaldehyde (Thermo Fisher, 28906) in PBS for 15 min at room temperature ...
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bioRxiv - Microbiology 2024Quote: Sorbitol-synchronized MitoRed or NF54 schizonts were stained with 0.5 μg/ml Hoechst 33342 (Invitrogen, H3570) for 30 minutes at 37 °C for nuclear staining ...
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bioRxiv - Microbiology 2024Quote: ... HPV16 was incubated with AF568 succinimidylester (Invitrogen) in a 1:8 molar L1:dye ratio in the dark for 1 h at RT while rotating ...
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bioRxiv - Microbiology 2024Quote: ... coli BL21 (DE3) Star (Invitrogen) using 2xTY media supplemented with kanamycin (50 μg/ml) ...
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bioRxiv - Microbiology 2024Quote: ... SYBR Green I (Fisher Scientific, Illkirch, France) at a 2X concentration in a lysis buffer (20 mM TRIS base ...
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bioRxiv - Microbiology 2024Quote: ... 1/1000 Alexa FluorTM 647-conjugated cross-absorbed goat anti-rabbit IgG (Invitrogen, A-21244) and 1/1000 Alexa FluorTM 488-conjugated cross-absorbed goat anti-human IgG (heavy chain specific ...
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bioRxiv - Microbiology 2024Quote: ... supplemented to give the following final concentrations: L-glutamine 2 mM (Gibco, 2530-081), glucose 16 mM ...
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bioRxiv - Microbiology 2024Quote: ... Enrichment due to recruitment of the regulator in vivo was determined using appropriate dilutions of IP DNA in a reaction buffer containing SYBR green mix (Invitrogen), 2 µM PAGE-purified primers and one unit of Platinum Taq DNA polymerase (Invitrogen) ...
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bioRxiv - Microbiology 2024Quote: ... falciparum strains were cultured at 2% haematocrit in O+ erythrocytes and RPMI-1640 medium (Lonza, BE12-167F or Gibco, 31870-025) supplemented to give the following final concentrations ...
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bioRxiv - Microbiology 2024Quote: ... The outlet of the HPLC system was connected to an electrospray ionization (ESI) source of an Orbitrap Fusion Tribrid Mass Spectrometer (Thermo Scientific). The instrument was operated in positive ESI MS-only mode for deuterated samples ...
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bioRxiv - Microbiology 2024Quote: ... Alfa-tubulin was labeled with an anti-alfa tubulin mouse antibody (1.500, Thermofisher) and chicken anti-mouse AlexaFluor 488 antibody (1:400 ...
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bioRxiv - Microbiology 2024Quote: ... Genomic DNA was extracted using Purelink Genomic DNA kit (Invitrogen K182001). Widened pipette tips were used to maintain higher molecular weight DNA ...
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bioRxiv - Microbiology 2024Quote: ... and lysed by re-suspending in 1 ml Trizol (Ambion) in the presence of Lysing Matrix B (100 µm silica beads ...
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bioRxiv - Microbiology 2024Quote: ... Use of platinum Taq DNA polymerase (Invitrogen) as control reactions ensured absence of genomic DNA in our RNA preparations ...
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bioRxiv - Microbiology 2024Quote: ... The cells were washed three times with 200 µl PBS and resuspended in 40 µl of PBS/1% BSA containing 1/2500 of VybrantTM DyeCycleTM Violet stain (Invitrogen, V35003) to stain parasite DNA ...
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bioRxiv - Microbiology 2024Quote: ... PClneo-EGFP incorporation was investigated by quantitative PCR (ABI7500, Applied Biosystems) using a plasmid standard to determine the number of pseudogenomes ...
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bioRxiv - Microbiology 2024Quote: ... the protocol from Invitrogen was altered as to not include any vortexing but instead shaking to prevent excess DNA shearing ...
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bioRxiv - Microbiology 2024Quote: ... the cells were stained with 150 nM Lyso-Tracker Red DND 99 (Invitrogen). Cells were fixed ...
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bioRxiv - Microbiology 2024Quote: ... tuberculosis were used to perform RT-qPCR using Superscript III platinum-SYBR green one-step kit (Invitrogen). cDNA was generated from 50 ng of RNA using Superscript III platinum enzyme ...
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bioRxiv - Microbiology 2024Quote: ... MitoTracker stainings (including MitoTracker™ Orange CMTMRos, Red CMXRos, Deep Red FM, Rhodamin123, all from ThermoFisher) were done by incubation of the parasites with 100 nM MitoTracker or 1 μg/ml Rhodamin123 for 30 minutes at 37 °C ...
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bioRxiv - Microbiology 2024Quote: ... 2 µM PAGE-purified primers and one unit of Platinum Taq DNA polymerase (Invitrogen). qPCR signal from a mock control lacking antibody was measured to determine the efficiency of recruitment ...
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bioRxiv - Microbiology 2024Quote: ... The IMC protein GAP45 was labeled using the anti-GAP45 rabbit antibody (1:5000) (a kind gift from Julian Rayner) and goat anti-rabbit AlexaFluor 488 antibody (1:500, Invitrogen). Alfa-tubulin was labeled with an anti-alfa tubulin mouse antibody (1.500 ...
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bioRxiv - Microbiology 2024Quote: ... MitoTracker stainings (including MitoTracker™ Orange CMTMRos, Red CMXRos, Deep Red FM, all from ThermoFisher) were done before settling and fixation by incubation of the parasites with 100 nM MitoTracker for 30 minutes at 37 °C ...
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bioRxiv - Microbiology 2024Quote: ... and chicken anti-mouse AlexaFluor 488 antibody (1:400, Invitrogen).
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bioRxiv - Microbiology 2024Quote: ... and Qubit fluorometer (Invitrogen). In all cases RNA preparations were free from genomic DNA.
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bioRxiv - Microbiology 2024Quote: ... AlbuMAX II Lipid-rich bovine serum albumin (BSA) (Gibco 11021037) 0.25% ...