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Citations for Thermo Fisher :
5201 - 5250 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: ... Pen/Strep 1X (Gibco)) ...
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bioRxiv - Developmental Biology 2024Quote: ... Sections were then washed with 0.05% PBT 3 times and incubated with appropriate Alexa Fluor-conjugated secondary antibodies (1:1,000 for Alexa 488 and Alexa 568 and 1:500 for Alexa 647; Thermo Fisher) in blocking buffer for 1 h at room temperature ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were next incubated in fluorescent secondary antibodies: AlexaFluor Plus 488 goat anti-mouse IgG (H + L) (1:500; A32723; ThermoFisher Scientific) and Texas Red Streptavidin conjugate (1:1000 ...
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bioRxiv - Neuroscience 2024Quote: ... and Texas Red Streptavidin conjugate (1:1000; S872; ThermoFisher Scientific). After a 2-h incubation ...
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bioRxiv - Developmental Biology 2024Quote: ... with an additional treatment with TurboDNase to eliminate all traces of genomic DNA (Invitrogen). Total RNA was then purified with Monarch RNA Cleanup Kit (NEB ...
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bioRxiv - Developmental Biology 2024Quote: ... The samples were electroporated in the MES buffer in 1 mm 4%-12% Bis-Tris gels (Invitrogen) and blotted onto PVDF membrane using the Trans-Blot Turbo Transfer System (Bio-Rad ...
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bioRxiv - Developmental Biology 2024Quote: ... and cultured in SCs culture medium (growth medium: 20% FBS, 5% horse serum, 1% penicillin/streptomycin, 1% GlutaMAX supplement (Gibco) and 2.5 ng/ml FGF (R&D systems ...
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bioRxiv - Developmental Biology 2024Quote: ... the Click-iT Reaction Kit (Thermo Fisher Scientific) was used before blocking according to the manufacturer’s recommendations.
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bioRxiv - Developmental Biology 2024Quote: ... TRIM66 was detected with secondary donkey Alexa Fluor 647 (Thermofisher) anti-mouse antibody at 1:1000 dilution and all histone marks with secondary donkey Alexa Fluor 546 (Thermofisher ...
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bioRxiv - Developmental Biology 2024Quote: ... in DMEM (Gibco)) at 37°C in tissue culture incubators with 5% CO2 ...
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bioRxiv - Cell Biology 2024Quote: ... U2OS cells were grown in McCoy’s 5A Medium (Gibco 16600-082) with 10% fetal bovine serum ...
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bioRxiv - Cell Biology 2024Quote: Cells cultured on #1.5 glass coverslips or Nunc™ Lab-Tek™II chamber slides (Cat # 154453, ThermoFisher Scientific) were washed with DPBS and fixed in 10% normal buffered formalin (Cat # HT501128 ...
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bioRxiv - Cell Biology 2024Quote: ... DMEM with 25mM HEPES (Gibco Cat. No. 12430-054) supplemented with 10% dialyzed FBS (Gibco A33820-01 ...
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bioRxiv - Developmental Biology 2024Quote: ... Phalloidin 647 (Thermo Scientific, A22287) 1:500 ...
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bioRxiv - Neuroscience 2024Quote: ... and B27 (Gibco™), followed by incubation in transfection media ...
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bioRxiv - Neuroscience 2024Quote: ... The primary cultures were maintained for 8 days in Neurobasal-A medium (Gibco™) supplemented with 1% rat serum (Biowest) ...
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bioRxiv - Neuroscience 2024Quote: ... DAPI (Invitrogen, D1306, 1:2000 dilution), 0.5% Triton X-100 ...
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bioRxiv - Cell Biology 2024Quote: ... in 6-well plates pre-coated with Geltrex (Cat # A1413302, ThermoFisher Scientific). Cells received daily media changes and were passaged every 4-5 days as aggregates using 0.5 mM EDTA in DPBS (Cat # AM9260G ...
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bioRxiv - Cell Biology 2024Quote: ... Cell lysate was immunoprecipitated using the indicated antibodies conjugated to Dynabeads™ Protein G (Cat # 10003D, ThermoFisher Scientific). Immunoprecipitated proteins were run on SDS-PAGE gels and evaluated by Western blot as described below.
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bioRxiv - Cell Biology 2024Quote: ... RNA concentration and quality were measured with a NanoDrop™ 2000 spectrophotometer (Cat# ND-2000, ThermoFisher Scientific) where an A260/280 ratio >2.0 indicated high quality RNA ...
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bioRxiv - Cell Biology 2024Quote: ... The concentration of extracted total RNA was measured using Qubit 3 Fluorometer (Thermo Fisher Scientific). Quality and integrity of RNA were measured using the Eukaryote Total RNA Nano assay on Bioanalyzer 2100 (Agilent Technologies) ...
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bioRxiv - Cell Biology 2024Quote: ... or RPMI 1640 media (ThermoFisher Scientific), both supplemented with 2% male AB human serum (Biowest Nuaillé ...
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bioRxiv - Cell Biology 2024Quote: ... Proliferation rate was determined by dye-dilution assay using CellTrace Violet (ThermoFisher Scientific). Cells were processed following manufacturer’s suggested procedure ...
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bioRxiv - Cell Biology 2024Quote: ... The membrane was washed twice for 5 min with TBST 0.01% Tween and incubated with anti-mouse HRP antibody (Invitrogen #31431) for 15 min at RT ...
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bioRxiv - Cell Biology 2024Quote: ... CD4+ T-cells were cultured in RPMI 1640 media (ThermoFisher Scientific), supplemented with 2% male AB human serum (Biowest Nuaillé ...
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bioRxiv - Cell Biology 2024Quote: ... Chemiluminescence detection was conducted using SuperSignal West Femto Maximum Sensitivity Substrate (34096, ThermoFisher Scientific) and Molecular Imager Gel Doc XR System (Bio-Rad).
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bioRxiv - Cell Biology 2024Quote: ... Immunocomplexes were visualized using the SuperSignal West Pico PLUS Chemiluminescent HRP substrate (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2024Quote: Cells cultured for 4 days at a starting density of 7 x 104/cm2 were lysed in TRIzol (Ambion) for 5 min and transferred to an RNA-free microcentrifuge tube following manufacturer recommendations ...
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bioRxiv - Cell Biology 2024Quote: ... 0.5% Bacto casamino acids (Thermo Fisher Scientific, Waltham, MA, USA), 2% glucose (Nacalai Tesque) ...
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bioRxiv - Cell Biology 2024Quote: ... DH-PH and VSV-G-DH-PH Rescue constructs were cloned using gateway recombination (Thermo Fisher) into pLenti CMV Hygro DEST (Addgene 17454) ...
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bioRxiv - Cell Biology 2024Quote: ... cDNA was synthesized using Superscript IV RT polymerase (Invitrogen), RNA pol inhibitor (Applied Biosystems) ...
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bioRxiv - Cell Biology 2024Quote: ... handpicked and subsequently maintained in CMRL-1066 (ThermoFisher Scientific) containing 5.6 mM glucose ...
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bioRxiv - Cell Biology 2024Quote: ... Resulting peptides were desalted with C18 StageTips (92) and analyzed by label-free quantitative mass spectrometry-based proteomics using a Q-Exactive Orbitrap mass spectrometer (Thermo Scientific, Germany) equipped with an EASY-nano liquid chromatography 1000 system (Proxeon ...
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bioRxiv - Cell Biology 2024Quote: ... MDCK cells were grown in DMEM (GIBCO) containing 10% FBS and antibiotics (penicillin-streptomycin) ...
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bioRxiv - Cell Biology 2024Quote: ... RNA pol inhibitor (Applied Biosystems), and 2000 ng of RNA as indicated by the manufacturer ...
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bioRxiv - Cell Biology 2024Quote: ... Affinity resin was prepared at room temperature: 100 μl Protein G Dynabeads (Invitrogen) were washed 3x in B-ChIP-BL (0.5 mg/ml BSA in PBS) ...
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bioRxiv - Cell Biology 2024Quote: ... PBMCs from the same donor were thawed to be stained with the CellTrace Violet (CTV) or CFSE Cell Proliferation kit (ThermoFisher Scientific) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... FM4–64 dye (Thermo Fisher Scientific) was added to log-phase cell cultures at a final concentration of 2 μM ...
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bioRxiv - Cell Biology 2024Quote: ... Complementary DNA using 0.1 to 1 µg RNA was synthesized using the Superscript III Reverse Transcriptase (Invitrogen-Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... 200 nM of primers and Power SYBR™ Green PCR Master Mix (Applied Biosystems) in a BioRad thermocycler ...
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bioRxiv - Cell Biology 2024Quote: ... and mounted on glass slides using ProLong Diamond Antifade Mountant (P36965; Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2024Quote: ... from Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... Both constructs were then electroporated into MDCK cells using the NeonTM transfection system (Thermo Fisher) and single cell clones isolated by serial dilution and checked for the fluorescent phenotype.
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bioRxiv - Cell Biology 2024Quote: ... Dynabeads M-280 Streptavidin (Thermo Fisher Scientific) was washed twice with liposome binding buffer ...
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bioRxiv - Neuroscience 2024Quote: ... the probe was soaked with a diluted solution of CM-DiI or DiA (ThermoFisher Product #V22888 or #D3883). The probe was manually lowered to the brain surface through a mixture of agar and PBS ...
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bioRxiv - Cell Biology 2024Quote: ... Complementary DNA using 0.1 to 1 µg RNA was synthesized using the Superscript III Reverse Transcriptase (Invitrogen-Thermo Fisher Scientific, Madrid, Spain). The QRT-PCR was performed on individual cDNAs using SYBR green (Roche ...
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bioRxiv - Developmental Biology 2024Quote: ... the expression vectors were linearized and in vitro transcribed using the SP6 message mMACHINE Kit (Invitrogen, AM1340). Poly(A ...
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bioRxiv - Cell Biology 2024Quote: ... for 1 h followed with phalloidin for staining F-actin (1:5000; #A22287 or #A12379, Thermo Fisher Scientific) for 30 min ...
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bioRxiv - Developmental Biology 2024Quote: ... Poly(A) tails (∼200–250 bp) were added to the transcribed mRNAs using a mMACHINE kit (Invitrogen, AM1350). The in vitro transcribed mRNAs were recovered using lithium chloride precipitation and resuspended in nuclease-free water ...
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bioRxiv - Cell Biology 2024Quote: Total protein was quantified using 1 ml cuvettes with a BCA Assay Kit (Thermo Scientific Pierce, 23225) according to the manufacturer’s instructions ...