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Citations for Thermo Fisher :
5351 - 5400 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S1 Sheep Fc Tag HEK293 Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... The neurons were washed in ice-cold PBS and biotinylated with 0.2 mg/mL biotin (Thermo Fisher, #21335) at 4°C for 15 min ...
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bioRxiv - Biophysics 2024Quote: ... CALHM2 and CALHM4 proteins were chemically biotinylated using amine-reactive EZ-Link™ NHS-PEG4-Biotin (ThermoFisher Scientific). For this purpose ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were treated with a final concentration of 50 μM biotin and 500 nM MitoTracker (Thermo Fisher, M7512), and exposed to 470 nm blue LED light for 2 hours in the incubator ...
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bioRxiv - Cell Biology 2023Quote: ... X-biotin-PE [N-((6-(biotinoyl)amino)hexanoyl)-1,2-dihexadecanoylsn-glycero-3-phosphoethanolamine] was obtained from Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2023Quote: Biotin-conjugated AT8 and HT7 are commercially available (AT8: Invitrogen, Cat. No. MN1020B; HT7: Invitrogen, Cat. No. MN1000B). T181 (abcam ...
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LRP1 mediates leptin transport by coupling with the short-form leptin receptor in the choroid plexusbioRxiv - Neuroscience 2023Quote: ... Cells were then biotinylated with EZ-Link™ Sulfo-NHS-SS-Biotin (0.3 mg/ml, Thermo Fisher Scientific) for 30 min at 4°C ...
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bioRxiv - Immunology 2023Quote: Cells were preincubated for 1 h with culture medium containing 250 μM biotin-glutathione ethyl ester (BioGEE, ThermoFisher), then treated as indicated ...
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bioRxiv - Immunology 2023Quote: ... Cells were then washed thrice in PBS and treated with 1 mg/ml Sulfo NHS-SS-Biotin (ThermoFisher) in biotinylation buffer (10 mM HEPES ...
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bioRxiv - Genomics 2022Quote: ... the RNA collected in each time point was biotinylated using the EZ-Link biotin HPDP kit (ThermoFisher, 21341) and biotinylated RNA was enriched with streptavidin-coated beads (DYNAL™ Dynabeads™ M-280 Streptavidin ...
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bioRxiv - Immunology 2023Quote: ... and SAv-Alexa Fluor 488 were mixed together with 5 µM free D-biotin (B20656; Thermo Fisher Scientific) to minimize cross-reactivity between probes ...
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bioRxiv - Cancer Biology 2023Quote: ... 4 × 108 cells were labeled with EZ-link Sulfo-NHS-SS-Biotin reagent (0.8mM, Cat. #21331, Thermo Scientific) for 30 minutes at room temperature ...
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bioRxiv - Biophysics 2023Quote: ... in which a quartz slide functionalized with PEG and PEG-biotin was coated with neutravidin (Thermo scientific, 31000), for approximately five minutes to allow the biotinylated DNA to bind a neutravidin immobilized on the slide ...
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bioRxiv - Plant Biology 2023Quote: ... The biotin signals on the membrane were detected using the chemiluminescent nucleic acid detection module (Thermo Fisher, 89880).
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bioRxiv - Neuroscience 2024Quote: ... It was followed by the wash and stain protocol (AF-901910 FlashTagTM Biotin HSR RNA Labeling Kit, Affymetrix). The whole procedure was performed using the GeneAtlas™ instrument from Affymetrix (#00-0393).
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bioRxiv - Microbiology 2023Quote: ... followed by staining with Goat anti-Rabbit IgG (H+L) Secondary Antibody-Biotin (Invitrogen, 65-614, 1:2000). Finally ...
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bioRxiv - Cancer Biology 2023Quote: ... 1% Triton X-100) in the presence of protease inhibitors and 0.1mM Iodoacetyl PEG2 biotin (IodoAPB, Fisher Scientific). Protein was quantified and 2mg input was used for protein enrichment by rotating for 1hr at RT to in 0.1mM IodoAPB to selectively label deprotonated selenocysteine residues ...
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bioRxiv - Immunology 2023Quote: ... post-dose 3 and 6-months post-dose 3 using mouse anti-human IgG1 biotin (Thermo Fisher Scientific) and mouse anti-human IgG4 biotin (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were incubated with a freshly prepared solution of 0.4 mM Sulfo-NHS-SS-Biotin (Thermo Fisher Scientific) in DPBS+ buffer for 30 min ...
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bioRxiv - Neuroscience 2024Quote: ... and treated with 1 mL of Sulfo-NHS-LC-Biotin EZ-Link (0.33 mg/mL, Thermo Scientific, #21335) for 10 min at 4°C ...
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bioRxiv - Plant Biology 2024Quote: ... Free biotin was removed from the samples using 10 ml ZebaTM Spin Desalting Columns 7K MWCO (Thermo Scientific). Bradford analysis was used to quantify protein concentration post desalting ...
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bioRxiv - Bioengineering 2021Quote: ... and incubated with secondary antibodies including DAPI (0.1 µg/ml) for 1 h at RT in the dark: Alexa Fluor 488 (Thermo Fisher Scientific, 1:300, sheep/mouse/rabbit), Alexa Fluor 568 (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... three times and incubated with horseradish peroxidase-conjugated secondary antibodies (Mouse, Cell Signaling, cat #7076S; Rabbit, Cell Signaling, cat #7074S; Rat, Cell Signaling, cat #7077S; Sheep, Thermo Fisher, cat #A16041) for 1 hour at room temperature ...
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bioRxiv - Biochemistry 2021Quote: PiMS data acquisition was performed in the individual ion mass spectrometry (I2MS) mode which has been described previously on a Q-Exactive Plus Orbitrap mass spectrometer (Thermo Fisher Scientific, Bremen, Germany, fig. S1) (48) ...
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bioRxiv - Genetics 2020Quote: ... Quantitative PCR reactions were performed using the gene-specific primers of FLC and FT (Table S1) with Hieff™ qPCR SYBR® Green Master Mix (Low Rox Plus, China) on a QuantStudio 6 Flex PCR (Life Technologies Corporation, CA, USA). The qPCR signals were normalized to that of the reference gene PP2A in Arabidopsis by using the ΔCT method ...
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bioRxiv - Plant Biology 2023Quote: ... listed in Table S1 were used in a 50 μl PCR reaction driven by Phusion™ High-Fidelity DNA Polymerase (Thermo Fisher Scientific, Waltham, MA, USA). The PCR conditions were ...
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bioRxiv - Immunology 2021Quote: ... Glucose uptake was measured by the uptake of glucose analogue 2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose (2-NBDG, Life Technologies). Cell surface and intracellular cytokine stainings of splenocytes and blood lymphocytes were performed as described (42) ...
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bioRxiv - Immunology 2021Quote: ... cells were incubated in glucose-free media containing 5 μg/ml 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG, Thermo Fisher) and 2.5% FBS at 37 °C ...
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bioRxiv - Physiology 2020Quote: ... 1 mM fluorescent glucose analog 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG, Life Technologies) was diluted in Live Cell Imaging Solution (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: Cellular glucose uptake was measured by culturing cells in glucose free DMEM for 2 hours before adding 100µM of the fluorescent glucose analogue 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl) Amino)-2-Deoxyglucose (2-NBDG; ThermoFisher, N13195). The analogue was incubated with MCF7s for 30 minutes and MSCs for 60 minutes at 37°C ...
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bioRxiv - Biophysics 2020Quote: MDA-MB-231 breast cancer cells were cultured in modified Eagle’s medium (MEM-F10, c.c.pro) supplemented with 10% fetal calf serum (FCS, Invitrogen) and 2.5 mM L-glutamin (c.c.pro ...
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bioRxiv - Biophysics 2020Quote: ... cell medium was replaced by 1 ml Leibovitz’s L-15 Medium (c.c.pro) containing 10% FCS and 25 nM Hoechst 33342 nucleic acid stain (Invitrogen) and incubated for 1 h at 37 °C before imaging.
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bioRxiv - Cancer Biology 2021Quote: ... After washing three times with MACS buffer consisting of PBS supplemented with 5 % FCS (Thermo Fisher Scientic) and 2 mM EDTA to remove unbounded cells ...
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bioRxiv - Immunology 2021Quote: ... and 10% fetal calf serum (FCS, Biowest) and minced into pieces of 1 mm3 in PBS (Gibco). The biopsies were enzymatically digested with 1 mg/mL collagenase IV (Sigma ...
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bioRxiv - Molecular Biology 2020Quote: ... USA) supplemented with 10% fetal calf serum (FCS) and 1% penicillin-streptomycin solution (Invitrogen, Carlsbad, CA, USA) and were incubated at 37□ in a humidified atmosphere containing 5% CO2 ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were pre-incubated with anti-Fc receptor antibody (Thermal Fisher Scientific, Cat. No. 14-9161-73) at 1:20 ratio to block Fc receptor before staining ...
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bioRxiv - Immunology 2021Quote: ... and the absorbance (OD492nm) was measured using a Multiskan-FC microplate reader (Thermo Fisher Scientific, Helsinki, Finland). Ligation of the recombinant proteins was compared to the SUMO control.
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bioRxiv - Immunology 2019Quote: ... resuspended in William’s E medium containing 10% FCS (PAA) and 1% Penicillin-Streptomycin-Glutamine (Thermo Fisher Scientific) and plated ...
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bioRxiv - Microbiology 2019Quote: ... in Dulbecco’s Modified Eagle medium (DME) supplemented with 1% v/v fetal calf serum (FCS) (Invitrogen, Australia) and 1% v/v Glutamax (Invitrogen ...
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bioRxiv - Developmental Biology 2020Quote: ... 1 % non-essential amino acids and sodium pyruvate and 30 % fetal calf serum (FCS) (Gibco, 10270-106). When cells reached confluency ...
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bioRxiv - Neuroscience 2020Quote: ... The absorbance at 450 nm wavelength was read on a microplate reader (Multiskan FC, Thermo Fisher Scientific). Mean values were calculated for triplicate sets of standards and samples ...
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bioRxiv - Biophysics 2020Quote: ... the substrate was washed with cell culture medium (DMEM w/glutamax, 10% FCS, 1% P/S; Gibco). 3T3 fibroblasts (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2020Quote: ... Absorbance was then measured at 595 nm using a Multiskan™ FC microplate photometer (Thermo Fisher Scientific).
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bioRxiv - Microbiology 2020Quote: ... supplemented with 10% FCS and antibiotics (100 units/mL penicillin, 100 mg/mL streptomycin, GIBCO, Life Technologies). Mammalian cells were incubated at 35°C in a humidified 5% CO2 incubator and insect cells at 28°C ...
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bioRxiv - Microbiology 2020Quote: ... supplemented with 10% FCS and antibiotics (100 units/mL penicillin, 100 mg/mL streptomycin, GIBCO, Life Technologies). Mammalian cells were incubated at 35°C in a humidified 5% CO2 incubator and insect cells at 28°C ...
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bioRxiv - Microbiology 2020Quote: ... the absorbance at 492 nm was measured with a microplate photometer (Multiskan FC, Thermo Fisher Scientific, USA).
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bioRxiv - Cell Biology 2021Quote: ... supplemented with 10% heat-inactivated fetal calf serum (FCS) (Cytiva) and 2mM L-glutamine (Thermo Fisher Scientific) at 37°C in humidified 5% CO2 ...
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bioRxiv - Immunology 2022Quote: ... the enzymatic dissociation of the lamina propria was quenched by addition of 1ml of 100% FCS (ThermoFisher) and 80 μL of 0.5M EDTA and placed on ice for five minutes ...
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bioRxiv - Immunology 2022Quote: ... After another round of Fc blocking on ice for 15 min with sera from mouse (Thermo Fisher) and rat (Thermo Fisher) ...
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bioRxiv - Developmental Biology 2022Quote: ... Cells were recovered in Hanks’ balanced-salt solution (HBSS) supplemented with 1% fetal calf serum (FCS) (Gibco) or RPMI medium (Gibco ...
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bioRxiv - Microbiology 2021Quote: Monocytic cell line U937 (ATCC® CRL1593.2™) cultured in RPMI (Thermoscientific) supplemented with 10% FCS (Invitrogen) was used in all experiments ...