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Citations for Thermo Fisher :
5201 - 5250 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S1 Sheep Fc Tag HEK293 Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... The cells were cultivated in DMEM-F12 medium containing 20% fetal bovine serum (FCS, Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... Proteins fused with Fc were captured using Pierce Protein A/G Plus Agarose (Thermo Scientific, 20424), eluted with pH 3.0 glycine (100 mM in H2O) ...
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bioRxiv - Systems Biology 2023Quote: MetR medium was prepared by titrating 0% MetR medium (complete DMEM, ThermoFisher, 21013-024, 10% FCS) with 100% MetR medium (complete DMEM without L-methionine and L-cysteine ...
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bioRxiv - Systems Biology 2023Quote: ... cells were washed and maintained in regular culture medium (DMEM GlutaMAX™, Gibco®, 10% FCS) for 5 days ...
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bioRxiv - Bioengineering 2023Quote: ... binding was quantified by measuring the fluorescence of a PE-conjugated anti-human Fc antibody (Invitrogen) detecting the Fc-fused protein target ...
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bioRxiv - Microbiology 2023Quote: Mono-Fc tagged PvDBP-RII used for SAXS experiments was expressed in Expi293 cells (Thermo Scientific) as described above and purified using the CaptureSelect C-tagXL Affinity Matrix (Thermo Scientific) ...
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bioRxiv - Immunology 2023Quote: ... blocked with Fc-block and stained with LiveDead APCe780 (1:1000; Thermo Fisher, #65-0865-14), APC anti-CD19 (1:200 ...
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bioRxiv - Bioengineering 2023Quote: ... HRP-conjugated goat anti-human IgG-Fc (10000x dilution) was used for detection (Invitrogen Cat# 31413). Immobilon Western Chemiluminescent HRP Substrate (Millipore Sigma ...
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bioRxiv - Microbiology 2023Quote: ... prior to resuspension in complete RPMI (with 10% FCS, 1% Penicillin/Streptomycin, 1% L-glutamine, Gibco) and passing through a 70 μm cell strainer ...
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bioRxiv - Immunology 2023Quote: ... After treating the cells for 15 minutes (min) with Fc Receptor Binding Inhibitor Polyclonal Antibody (ThermoFisher), they were stained for 30 min at 4°C in the dark and washed twice again ...
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bioRxiv - Immunology 2023Quote: ... Optic density was then measured at 405 nm on a Multiskan FC microplate photometer (Thermo Scientific).
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bioRxiv - Cancer Biology 2023Quote: ... and -10 Fcs were assessed by detecting with an anti-mouse IgG-specific secondary (Thermo Fisher) and analyzed via flow cytometry (BD Biosciences).
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bioRxiv - Bioengineering 2023Quote: Ly6a-Fc was produced in Expi293F suspension cells grown in Expi293 Expression Medium (Thermo Fisher Scientific) in a 37 °C ...
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bioRxiv - Bioengineering 2023Quote: Ly6a-Fc was produced in Expi293F suspension cells grown in Expi293 Expression Medium (Thermo Fisher Scientific) in a 37 °C ...
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bioRxiv - Cancer Biology 2023Quote: ... medium of the HEK293T cells was changed to transfection medium containing DMEM and 10% FCS (Gibco), incubating the cells for 30 min at 37 °C ...
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bioRxiv - Bioengineering 2023Quote: ... The sections were then incubated with WA2.22 Fc fusion (100 nM) and AT8 (1:200, Invitrogen) overnight at 4 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... KELLY cells were cultured in RPMI medium with 10-15% fetal calf serum (FCS) (Life Technologies). Cells were maintained at 5% CO2 and at 37°C and were split at full confluency (at 5% CO2 and at 37°C ...
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bioRxiv - Immunology 2023Quote: ... cells were first incubated with an anti-CD16/CD32 Monoclonal Antibody to block FC receptors (Invitrogen). Cells were washed using PBS and incubated with LIVE/DEAD™ dye for 30 minutes at 4°C ...
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Shear stress and very low levels of ligand synergize to activate ALK1 signaling in endothelial cellsbioRxiv - Cell Biology 2023Quote: ... ALK1-Fc-complexes were then captured with CaptureSelect FcXL Affinity Matrix (Cat: 194328010, Thermo Fisher Scientific). The ALK1 ligand-depleted 30% BSA was diluted to 0.2% with phenol red-free EBM2 for use.
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bioRxiv - Immunology 2023Quote: ... Raji cells and CHO cells were cultured in complete RPMI-1640 (Gibco, 10% FCS, 2mM GlutaMAX), and HEK293T cells ...
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bioRxiv - Immunology 2023Quote: ... prior to resuspension in complete RPMI (with 10% FCS, 1% Penicillin/Streptomycin, 1% L-glutamine, Gibco) and passing through a 70 μm cell strainer ...
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bioRxiv - Microbiology 2023Quote: Isolated monocytes were resuspended in complete RPMI media with 2mM L-glutamine and 5% FCS (Gibco) and seeded in a 24-well cell culture plate at 5 x 105 cells/well ...
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bioRxiv - Developmental Biology 2023Quote: ... Optical density values were determined using a Multiskan™ FC Microplate Photometer (Thermo Scientific™ 51119000).
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bioRxiv - Neuroscience 2023Quote: ... supplemented with 10% (v/v) fetal calf serum (FCS) (Gibco A5670401; Thermo Fisher Scientific, Waltham, MA) and 1% (v/v ...
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bioRxiv - Synthetic Biology 2024Quote: ... after which adsorption was measurement at 450 nm using a microplate reader (Multiscan FC, Thermo Scientific).
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bioRxiv - Microbiology 2024Quote: ... ∼300RU of each Fc-tagged KIR2DL1 and KIR2DS1 were captured via Protein A/G (ThermoFisher Scientific) pre-immobilized on a CM5 sensor (∼1500 RU on each flow cell) ...
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bioRxiv - Microbiology 2024Quote: ... supplemented with 10 % (v/v) FCS and 0.5 % (v/v) Insulin-Transferrin-Selenium (Thermo Fisher Scientific). For the experiment ...
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bioRxiv - Immunology 2024Quote: ... prior to resuspension in complete RPMI (with 10% FCS, 1% penicillin/streptomycin, 1% L-glutamine; Gibco) and passing through a 70 µm cell strainer ...
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bioRxiv - Immunology 2024Quote: ... supplemented with 10% fetal calf serum (FCS) (#S181BH-500, Biowest) and 1% penicillin-streptomycin (#15140122; Gibco). Purity > 97% of CD14+ monocytes after magnetic sorting was verified by flow cytometry (anti-CD14-FITC clone MφP9 ...
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bioRxiv - Bioengineering 2024Quote: ... 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG, Invitrogen, Cat # N13195) and incubated for 30 minutes in their respective culture conditions ...
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bioRxiv - Microbiology 2021Quote: ... the -10 and -35 consensus sequences of the J23119 promoter and the lpp-sgRNA chimera (Table S1) was synthesized by GeneArt (Thermo Fisher Scientific, Waltham, MA, USA) and cloned in pCRISPR-sacB using AvrII and XhoI restriction sites ...
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bioRxiv - Biochemistry 2021Quote: ... For the FAIMS CV scanning of synthetic peptides and glycopeptides, each sample (Table S1, Supporting Information) was directly injected to the Orbitrap Lumos (Thermo Fisher Scientific, San Jose, CA). The CV was scanned from 0-100 V in 1 V steps ...
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bioRxiv - Microbiology 2022Quote: ... The titer of the applied FVT virome was approximately 5.4 × 109 virus-like particles (VLP)/mL (Figure S12 and Table S1) for both 1st and 2nd inoculation and was evaluated by epifluorescence microscopy stained by SYBR™ Gold (Thermo Scientific, cat. no. S11494) as previously described (http://dx.doi.org/10.17504/protocols.io.bx6cpraw) ...
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bioRxiv - Zoology 2024Quote: ... with optimized primers designed using Geneious Pro Bioinformatics Software (Supplement Table S1) on a StepOnePlus Real-Time PCR System (Applied Biosystems, Carlsbad, CA, United States) using PowerUPTM SYBR® Green Master Mix (Applied Biosystems ...
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bioRxiv - Microbiology 2023Quote: ... PCR fragments were amplified using appropriate primer pairs (Table S1) and Phusion High-Fidelity PCR Master Mix with GC Buffer (Thermo Scientific, Walthman, MA, United States). Linear fragments were purified on agarose gels and inserted into pGMCS backbone linearized by PCR amplification with appropriate primers ...
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bioRxiv - Neuroscience 2022Quote: ... 100 μM 2-mercaptoethanol (2-ME, Gibco) and 1 × Antibiotic-Antimycotic (Gibco ...
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bioRxiv - Cell Biology 2019Quote: ... 2×2 binning (3.7mm^2 field area)) on the ArrayScan VTI (Thermo Scientific) and analyzed simultaneously for percentage of GFP positive cells using the Target Activation Bioapplication (Thermo Scientific ...
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bioRxiv - Microbiology 2022Quote: ... of the SARS coronavirus strain Tor2 (NC_004718.3) was ordered as a synthetic DNA string fragment (GeneArt synthesis; Thermo Fisher Scientific, Darmstadt, Germany) and cloned into the pEXPR103 expression vector (IBA Lifesciences ...
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bioRxiv - Microbiology 2021Quote: 5×106 HEK 293T cells were co-transfected with 6 μg each of pNL4-3.Luc.R-E-and recombinant plasmid (pS-SARS, pS-MERS, pS-D614, pS-G614, pS-Variant1, or pS-Variant2) using Lipofectamine 3000 Transfection Reagent (Invitrogen, Rockville, MD) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: Exosomes were isolated from 300 µl of plasma from 30 healthy individuals and 30 subjects with SARS-CoV2 infection using “Total Exosome Precipitation Reagent” from Invitrogen (Carlsbad, CA). Briefly ...
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bioRxiv - Immunology 2021Quote: ... cells were incubated in 2-(N-(7-nitrobenz-2-oxa-1,3-diaxol-4-yl) amino)-2-deoxyglucose (2-NBDG) (Invitrogen) (20 µM ...
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bioRxiv - Physiology 2021Quote: ... 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG, Thermo Fisher Scientific, USA) was dissolved in saline solution at 5 mg/ml ...
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bioRxiv - Cancer Biology 2020Quote: ... (2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG) (100 μM; ThermoFisher Scientific) with Hoechst (1 μg/mL ...
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bioRxiv - Physiology 2019Quote: ... 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG, Thermo Fisher Scientific, USA) was dissolved in PBS at 5 mg/ml ...
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bioRxiv - Immunology 2020Quote: ... Glucose uptake assay was performed by incubating cells with 50 µM 2-NBDG (2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose) (Invitrogen) for 90 min at 37°C prior to cell staining ...
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bioRxiv - Immunology 2022Quote: ... cells were resuspended in glucose-free media containing 150 μM 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG Thermofisher) and incubated for 45 minutes at 37°C.
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bioRxiv - Microbiology 2023Quote: ... CoV RNA was detected using a pancoronavirus nested PCR with primers targeting the conserved RdRp gene of CoVs as previously published (Watanabe et al., 2010) and using the Platinum II Taq Hot-Start DNA Polymerase (Thermo Fisher Scientific). After PCR ...
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bioRxiv - Microbiology 2021Quote: ... The supernatant was diluted 1:1 with cold H2O and loaded onto a C-tag Affinity Column (ThermoFisher) equilibrated with C-tag Binding Buffer ...
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bioRxiv - Cancer Biology 2022Quote: ... Desalted peptides were then labeled with 10-plex Tandem-mass-tag (TMT) reagents following manufacturer’s instructions (Thermo Scientific). The samples were then reconstituted in volume of 20 mM ammonium formate (pH 10 ...
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bioRxiv - Microbiology 2019Quote: ... anti-V5 (1:50 or 1:100; V5 Tag Monoclonal Antibody, Cat. Nr. R960-25, Thermo Fisher Scientific) or self-made antibodies raised against GlCHC (dilution 1:1000 ...