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Citations for Thermo Fisher :
5301 - 5350 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S1 Sheep Fc Tag HEK293 Biotin since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2021Quote: ... Biotin-labeled DNA was purified by incubation with 100µl Dynabeads MyOne Streptavidin C1 beads (Thermal Fisher Scientific, 65002) at RT for 15min ...
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bioRxiv - Cell Biology 2020Quote: ... pH 7.5 in 1X PBS then treated with 400 μg/ml sulfo-N-hydroxysulfosuccinimide-biotin (Thermo Fisher Scientific) prepared in the washing buffer for 40 min on an orbital shaker at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... pH 7.4) and incubated with 0.5 mg ml−1 sulfo-NHS-LC-biotin (EZ-Link, Pierce/Thermo Scientific) in PBS2+ while shaking for 20 min at 4 °C ...
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bioRxiv - Molecular Biology 2020Quote: ... using the Applied Biosystems™ FlashTag™ Biotin HSR RNA Labeling Kit (ThermoFisher Scientific, Waltham, MA, the # 902446), 400 ng of total RNA was labeled with biotin and hybridized to the miRNA 4.0 Array (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... the purified RNAs were labeled with biotin using a Pierce RNA 3’ End Biotinylation kit (20160, Thermo Scientific) according to the manufacturer’s manual ...
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bioRxiv - Cell Biology 2019Quote: ... αCT1 eluted from cross-linking reactions was detected on blots against biotin with HRP-NeutrAvidin (ThermoFisher, 31001, MA). Signals were detected by HR-based chemiluminescence (ThermoFisher ...
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bioRxiv - Biophysics 2019Quote: ... Nick translation was initiated by the addition of 5 µl of 250 µM biotin-11-dUTP (ThermoFisher Scientific) and 1.5 U of E.coli DNA polymerase (New England Biolabs ...
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bioRxiv - Molecular Biology 2019Quote: ... 10mM EDTA) and 20% EZ-Link Biotin-HPDP (1 mg/mL resuspended in DMF, Thermo Fisher Scientific, 21341) for 1.5 hours at 800 rpm and 24°C in the dark ...
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bioRxiv - Molecular Biology 2019Quote: ... We added 52 µL of incorporation master mix: 37.5 µL of 0.4 mM biotin–dATP (Thermo Fisher, 19524016); 4.5 µL of a dCTP ...
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bioRxiv - Cell Biology 2019Quote: ... in PBS was incubated with 86.1μl of Sulfo-NHS-SS-Biotin (sulfosuccinimidyl-20(biotinamido)ethyl-1,3-dithiopropionate) (8mM) (Thermo Scientific) for 2 hrs at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... Surface proteins were labelled with EZ-Link Sulfo-NHS-LC-Biotin (Thermo Scientific, 0.3 mg/ml in PBS) at 4 °C for 30 min ...
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bioRxiv - Evolutionary Biology 2020Quote: ... A small amount of the purified proteins were biotinylated using the EZ-link BMCC-biotin system (ThermoFisher Scientific). ≈1 mg BMCC-biotin was dissolved directly in 100% DMSO to a concentration of 8 mM for labeling ...
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Enzymatic RNA Biotinylation for Affinity Purification and Identification of RNA-protein InteractionsbioRxiv - Biochemistry 2020Quote: ... Blot was developed for biotin detection using the Chemiluminescent Nucleic Acid Detection Module Kit (Thermo Scientific, Waltham, MA)
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bioRxiv - Neuroscience 2021Quote: ... 260 ng of total RNA was labeled using the FlashTag Biotin RNA Labeling Kit (Affymetrix, Santa Clara, CA). Labeled samples were hybridized to the arrays at 48°C for 20h and then washed and stained with a Streptavidin-PE solution ...
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bioRxiv - Neuroscience 2020Quote: ... Antibody biotinylation was performed according to manufacturer’s instructions (EZ-Link Sulfo-NHS-Biotin, Thermo Scientific, cat. no. 21217), after BSA removal with the Melon Gel IgG Purification Kit (Thermo Scientific ...
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bioRxiv - Physiology 2020Quote: ... cells were incubated with the cleavable EZ-Link Sulfo-NHS-SS-Biotin (0.5 mg/ml, Thermo Fisher Scientific) in ice-cold PBS (pH 7.4 ...
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bioRxiv - Cell Biology 2021Quote: ... or 5 µl of EZ-Link™ Sulfo-NHS-Biotin (21217, Thermo Fisher Scientific, 1 µg/20 µl), while still attached via the umbilical cord to the mother’s blood circulation ...
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bioRxiv - Plant Biology 2021Quote: ... labelled with the ENCORE Biotin Module (NuGEN) and hybridized to Arabidopsis Gene 1.1 ST arrays (Affymetrix, CA, USA) according to the manufacturers protocol ...
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bioRxiv - Genomics 2021Quote: Fragment ends were filled in by adding 37.5 μL of 0.4 mM biotin-14-dATP (ThermoFisher Scientific, # 19524016), 1.5 μL each of 10 mM dCTP ...
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bioRxiv - Molecular Biology 2021Quote: ... The purified PCR products were 3’-end-labelled with biotin following the manufacturer’s instruction (Thermo Fisher Scientific, USA). The DNA-protein binding reactions were performed according to the manufacturer’s instructions (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: Cultured astrocytes were incubated in 1 mg/ml cell-impermeant EZ-Link Sulfo-NHS-SS-Biotin (Thermo Fisher) in ice-cold PBS pH 8 for 2 h ...
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bioRxiv - Molecular Biology 2022Quote: ... Biotin-labelled RNA was purified with a 1:1 ratio of Phenol/Chloroform/Isoamyl alcohol (Thermo Fisher Scientific). Streptavidin pull-down was performed with the μMACS Streptavidin Kit (Miltenyi Biotec) ...
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bioRxiv - Biophysics 2022Quote: BSA-pre-structured wells were incubated with biotin-BSA (0.1 mg/ml, Thermo Fisher Scientific, Waltham, MA, USA) and SA (1 μM ...
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bioRxiv - Neuroscience 2022Quote: ... according to the manufacturer’s protocol in order to label the AHA-incorporated proteins with biotin alkyne (ThermoFisher, B10185). The Click reaction samples were incubated on a rotator at 4 °C for 1 h ...
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bioRxiv - Immunology 2022Quote: ... after which excess biotin was removed using a 40K MWCO z spin desalting column kit (ThermoFisher, catalog #87766).
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bioRxiv - Neuroscience 2022Quote: ... An aliquot was removed (total fraction) before proceeding with the biotin (luminal) fraction purified on NeutrAvidin (Thermo Fisher) columns (Pierce) ...
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bioRxiv - Cancer Biology 2022Quote: ... The fragment was labeled with biotin using the Pierce™ RNA 3′ End Desthiobiotinylation Kit (Thermo Fisher Scientific), and RNA pull-down was performed using the Magnetic RNA Protein Pull-Down Kit (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... diluted at 1:300 followed by 1hr incubation with avidin-biotin complex (1:1:100; cat# 32020; ThermoFisher) and 1hr incubation with TSA plus Cyanine-3 (1:300 ...
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bioRxiv - Genetics 2022Quote: ... Biotin probes were captured using 125 μL of pre-washed Streptavidin beads (RNAse clean C1 beads, Life Technologies). The hybridized RNA/probe bead mixture was agitated on a rotator at room temperature for 20 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... fibronectin was biotinylated by incubating 0.1mg/mL fibronectin with 0.5mM EZ-Link Sulfo-NHS-LC-Biotin (A39257, ThermoFisher) for 30 min at RT ...
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bioRxiv - Genomics 2020Quote: ... The biotin-labeled DNA was sheared and pulled down using Dynabeads MyOne Stretavidin T1 beads (Life Technologies, 65602) and prepared for Illumina paired-end sequencing ...
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bioRxiv - Genomics 2019Quote: ... A 100-µl volume of 100 mM NaPi buffer containing 20 mM amine-PEG2-biotin (Thermo Fisher Scientific) was added and the mixture was heated to 75 °C for 10 min ...
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bioRxiv - Neuroscience 2019Quote: ... We purified biotin-containing proteins with Dynabeads (150 μl per reaction; Dynabeads M-280 Streptavidin-Thermo Fisher Scientific). Dynabeads were separated over a magnetic rack and subsequently washed 2 times in PBS containing 0.1% Bovine Serum Albumin (BSA) ...
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bioRxiv - Cell Biology 2021Quote: ... for microscopy slides or 0.8 mg/ml PEG-Silane and 0.2 mg/ml Biotin-PEG-Silane (5 K, Creative PEGWorks) for coverslips and 0.05 mg/ml NeutrAvidin (Invitrogen) in G-Buffer (2 mM Tris-Cl pH 8 ...
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Structure-guided glyco-engineering of ACE2 for improved potency as soluble SARS-CoV-2 decoy receptorbioRxiv - Biochemistry 2021Quote: ... rshACE2 and deglyco-rshACE2) were biotinylated using the EZ-Link Sulfo-NHS-LC-Biotin kit (Thermo Fisher Scientific). Excess sulfo-NHS-LC-biotin was quenched by adding Tris-HCl buffer (800 mM ...
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Force and stepwise movements of gliding motility in human pathogenic bacterium Mycoplasma pneumoniaebioRxiv - Microbiology 2021Quote: The cell suspension was mixed with 0.5 mM Sulfo-NHS-LC-LC-biotin (Thermo Fisher Scientific, Waltham, MA) as the final concentration and incubated for 15 min at room temperature (RT) ...
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bioRxiv - Cell Biology 2021Quote: ... Primary antibodies against Cx43 (South San Francisco, CA; Cat: SC6560) and biotin (Invitrogen, Carlsbad, CA ; Cat: 617-300) were applied under standard conditions ...
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bioRxiv - Genomics 2020Quote: ... Biotin labelled fragments which contained the 5’ and 3’ connecting region was enriched using Dynabeads M280 streptavidin (Invitrogen) and incubating at room temperature for 30 min ...
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bioRxiv - Neuroscience 2021Quote: ... pH 7.2) and surface proteins were biotinylated using 0.5 mg/ml Sulfo-NHS-SS-Biotin (Thermo Fisher Scientific) in PBS for 40 min at room temperature ...
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bioRxiv - Biochemistry 2022Quote: ... the cells were incubated for 30 minutes with 0.5 mg/ml sulpho-NHS-SS-biotin (Thermo Fisher Scientific) in PBS++ ...
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bioRxiv - Neuroscience 2022Quote: ... They were then incubated with Premium Grade EZ-link Sulfo-NHS-LC-Biotin (Thermo Scientific; 1 mg/ml) in modified HBSS for 30 min at RT ...
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bioRxiv - Neuroscience 2022Quote: Human BDNF-biotin (50 nM) was incubated with 50 nM Qdot-605 Streptavidin conjugate (Thermo Fisher Scientific; Q10101MP) on ice for 1 h in Neurobasal medium to facilitate the biotin-streptavidin interaction ...
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bioRxiv - Genomics 2022Quote: ... The exposed DNA ends were repaired and covalently linked with biotin-14-dCTP (Invitrogen Life Technologies, Carlsbad, CA), and then connected by T4 DNA ligase (Invitrogen Life Technologies ...
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bioRxiv - Genomics 2022Quote: ... The exposed DNA ends were repaired and covalently linked with biotin-14-dCTP (Invitrogen Life Technologies, Carlsbad, CA), and then connected by T4 DNA ligase (Invitrogen Life Technologies ...
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bioRxiv - Microbiology 2023Quote: ... the synthesized RNA was purified and biotin-labeled using Pierce RNA 3’ End Biotinylation Kit (Thermo Fisher Scientific). The obtained biotin-labeled RNA was used as an RNA probe ...
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bioRxiv - Immunology 2024Quote: ... 7.5 x 108 conidia were incubated in 10 μg/mL EZ-Link™ Sulfo-NHS-LC-Biotin (ThermoFisher) in 1 mL of 50 mM NaHCO3 buffer (pH 8.3 ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were cell-surface biotinylated with 0.1 mg/ml Sulfo-NHS-LC Biotin (Thermo Fisher Scientific, Waltham, MA) in HBSM (20 mM HEPES pH 7.2 ...
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bioRxiv - Molecular Biology 2022Quote: ... The protein ligands (Red1288-345 and Iss1) were biotinylated using EZ-Link NHS-PEG4-Biotin (Thermo Fisher Scientific). Biotin-labeled proteins were immobilized on the streptavidin (SA ...
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bioRxiv - Cell Biology 2023Quote: ... 10mM EDTA) and 20% EZ-Link Biotin-HPDP (1 mg/mL resuspended in DMF, Thermo Fisher Scientific, 21341) for 1.5 hours 24°C in the dark and 800 rpm to mix ...
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bioRxiv - Cell Biology 2023Quote: ... the fragmented biotin-labeled cDNA was synthesized from the extracted RNA according to ABI protocol (Thermo Fisher Scientific) and hybridized to GeneChips (Mouse Clariom S) ...