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Citations for Thermo Fisher :
4901 - 4950 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... Lysis was achieved by repeated freeze-thaw cycles followed by a spin at 13,000g for 2 minutes and a wash with methanol (ThermoFisher) to collect the supernatant fraction as lysate ...
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bioRxiv - Microbiology 2024Quote: ... ∼100,000 human bladder epithelial cells (RRID: CVCL_0126) were seeded in 24-well plates overnight in RPMI (Gibco; ThermoFisher Scientific) supplemented with 10% heat inactivated FBS ...
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bioRxiv - Microbiology 2024Quote: ... Library amplification was performed using Platinum Hot Start PCR 2x Master Mix from Invitrogen by Thermo Fisher Scientific using forward (515F ...
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bioRxiv - Microbiology 2024Quote: Human bladder epithelial cell line 5637 (RRID: CVCL_0126) were cultured and maintained in RPMI medium (Gibco; ThermoFisher Scientific) supplemented with 10% heat inactivated FBS (Sigma ...
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bioRxiv - Microbiology 2024Quote: ... 10μg of each sample was separated by SDS-PAGE using a 10% Bis-Tris NuPAGE gel (Invitrogen) with the MES buffer system ...
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bioRxiv - Microbiology 2024Quote: Recombinant proteins were diluted with Bicarbonate/carbonate coating buffer (100mM) at 0.2 ug/mL and 100μL/well was added to Polysoap Immunoplate (Nunc). The recombinant protein was immobilized for 2 days at 4 °C ...
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bioRxiv - Microbiology 2024Quote: ... The DynaMag™-2 magnetic rack (Thermo Fisher Scientific) was used for flow-through removal and washing ...
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bioRxiv - Microbiology 2024Quote: Peptides were introduced to the mass spectrometer using a Ultimate 3000 HPLC system (Dionex/Thermo Fisher Scientific)equipped with an Easy-Spray PepMap® RSLC column(50 cm × 75 μm inner diameter ...
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bioRxiv - Microbiology 2024Quote: ... The Q-Exactive mass spectrometer (Thermo Fisher Scientific) was operated in data-dependent mode with survey scans acquired at a resolution of 70,000 ...
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bioRxiv - Microbiology 2024Quote: ... Protein purification was performed using Protein A/G Plus Agarose (Thermo Fisher Scientific) for RBD and Strep-Tactin®XT 4Flow® high capacity resin (IBA ...
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bioRxiv - Cancer Biology 2024Quote: ... MLO-A5 and MLO-Y4 cells were purchased from the American Type Culture Collection (Rockville, MD, United States) and grown in dulbecco’s modified eagle medium (DMEM) (Invitrogen, Waltham, MA, United States) containing 10% FBS and 1% penicillin and streptomycin ...
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bioRxiv - Cancer Biology 2024Quote: ... +0.5µl 10mg/mL salmon sperm DNA (Thermo Fisher Scientific, cat# 15632011) and 20pmol probes with distinct fluorophores ...
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bioRxiv - Cancer Biology 2024Quote: ... Then coverslips were mounted on slides with Prolong Diamond Antifade Mountant (Thermo Fisher Scientific Cat#P36961) for imaging acquisition.
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bioRxiv - Cancer Biology 2024Quote: ... supplemented with 0.5µl 10mg/mL RNaseA (Thermo Fisher Scientific, cat# 12091021) +0.5µl 10mg/mL salmon sperm DNA (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: Genomic DNAs was extracted from normal and tumor samples using The GeneJet DNA kit (Thermo Fisher), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... was maintained in Minimum Essential Medium with Earlès salts and 2.0 mM L-Glutamine (MEM; Gibco). All cells were cultured at 37℃ in 5% CO2 with regular passage every 2-3 days.
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bioRxiv - Immunology 2024Quote: ... was fluorescently labelled using the Alexa Fluor 488 Antibody Labelling kit (Life Technologies)102 ...
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bioRxiv - Immunology 2024Quote: ... using SYBR Green (Power SYBR Green, Applied Biosystems). Ct values were read and exported from the QuantStudio software ...
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bioRxiv - Immunology 2024Quote: ... Cells were activated with soluble anti-CD3 (30 ng/mL, ThermoFisher) and anti-CD28 (30 ng/mL ...
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bioRxiv - Immunology 2024Quote: ... and complementary DNA (cDNA) synthesis was performed using the High-Capacity cDNA reverse transcription kit (Thermo Fisher Scientific) according to the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2024Quote: ... 1 mM DTT) with 1 U of RNase inhibitor per sample (RiboLock, Thermo Fisher Scientific). Cells were mechanically disrupted using a pre-chilled Precellys 24 (Bertin Instruments ...
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bioRxiv - Microbiology 2024Quote: ... The multi-host vector pVZ322 [65] was linearized using the restriction enzymes XbaI and XhoI (Thermo Fisher Scientific). Expression cassettes of ssr1238 and sfgfp were amplified by PCR ...
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bioRxiv - Cancer Biology 2024Quote: ... All co-culture experiments were conducted in DMEM with sEVs-free FBS (Life Technologies, Carlsbad, CA, United States).
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bioRxiv - Cancer Biology 2024Quote: ... supplemented with 20 ng/ml human epidermal growth factor (Invitrogen, PHG0311), 20 ng/ml human basic fibroblast growth factor (Stemcell Technologies ...
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bioRxiv - Microbiology 2024Quote: ... the peptide concentration was measured using the BCA assay (Thermo Scientific). For LC-MS/MS measurements 800 ng of peptides were analyzed on a Q- Exactive Plus mass spectrometer (Thermo Scientific ...
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bioRxiv - Microbiology 2024Quote: ... Peptide separation was carried out using an Ultimate 3000 nanoLC-system (Thermo Fisher Scientific, Waltham, USA), equipped with an in-house packed C18 resin column (Magic C18 AQ 2.4 µm ...
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bioRxiv - Microbiology 2024Quote: ... Extracted and desalted [45] peptides were analysed on an Easy-nLC 1200 system coupled to a Q Exactive HF mass spectrometer (all Thermo Fisher Scientific) [44] ...
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bioRxiv - Cancer Biology 2024Quote: ... the coverglass was mounted with ProLong Glass Antifade Mount (Invitrogen, P36984). Confocal imaging was performed on a Zeiss LSM780 or a Leica Stellaris 5 confocal microscope (63X/1.40 oil objective).
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bioRxiv - Cancer Biology 2024Quote: ... Alexa Fluor-conjugated goat anti-mouse or donkey anti-rabbit IgG (H+L) secondary antibodies were incubated together with Alexa Fluor-conjugated phalloidin (Invitrogen, A12380 or A22287) for 1 hr at RT ...
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bioRxiv - Microbiology 2024Quote: ... The corresponding PCR products were subjected to DpnI digestion (Thermo Fisher Scientific), 5’ phosphorylation by T4 polynucleotide kinase (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2024Quote: ... Quantity and purity of DNA isolated from bulk soil samples were assessed using a NanoDrop 8000 spectrophotometer (Thermo Fisher Scientific). The DNA was then amplified in triplicates ...
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bioRxiv - Microbiology 2024Quote: ... self-ligation by T4 DNA ligase (Thermo Fisher Scientific) and heat shock transformation into chemically competent DH5α E ...
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bioRxiv - Cancer Biology 2024Quote: ... was used in conjunction with the QuantiGene Sample Processing Kit for cultured cells (ThermoFisher Scientific), and QuantiGene Plex Assay kit (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... and QuantiGene Plex Assay kit (ThermoFisher Scientific) to quantify transcripts following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... A custom QuantiGene Plex panel (ThermoFisher Scientific) was used in conjunction with the QuantiGene Sample Processing Kit for cultured cells (ThermoFisher Scientific) ...
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bioRxiv - Cancer Biology 2024Quote: ... or K562 cells were electroporated (2 x 105 cells per reaction) with RNP complexes using the Neon Transfection System (Thermo Fisher Scientific) with the following parameters using Buffer R in 10 µL reactions ...
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bioRxiv - Cancer Biology 2024Quote: ... were formed by incubating 90 pmol sgRNA and 10 pmol Cas9-2NLS in Buffer R (Component of Neon Transfection System Kit; Thermo Scientific) at room temperature for 10 minutes ...
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bioRxiv - Cancer Biology 2024Quote: ... and protein quantified by Pierce BCA Protein Assay (Thermo Scientific). Protein samples were diluted to equal concentrations in lysis buffer and boiled for 5 minutes in 1X Laemmli Sample Buffer ...
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bioRxiv - Cancer Biology 2024Quote: ... The Average Net Mean Fluorescence Intensity was read on a Luminex FLEXMAP 3D System (Invitrogen). Signals from RPGs were normalized internally to those from GAPDH ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were counterstained with Hoechst (ThermoFisher, H3570), washed with PBS then mounted with ProLong Antifade Gold (ThermoFisher) ...
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bioRxiv - Neuroscience 2024Quote: ... cDNA was synthesized using a High-Capacity RNA-to- cDNA™ Kit (4387406, Applied Biosystems, USA) following the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2024Quote: ... We dissected the larval whole brains (at 6 or 13 dpf) in the RNAlater® (AM7021, Ambion, USA) after overnight incubation and the adult brains (at 120 dpf ...
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bioRxiv - Neuroscience 2024Quote: ... The real-time qPCR was performed with PowerUp™ SYBR™ Green Master Mix (A25778, Applied Biosystems, USA) and specific primers ...
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bioRxiv - Microbiology 2024Quote: ... 4’,6’-diamino-2-fenil-indol (DAPI) (1:25000, Life Technologies) and Phalloidin (Alexa 488 ...
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bioRxiv - Immunology 2024Quote: We analyzed the samples using an EASY-nLC 1200 (Thermo Fisher Scientific) connected to Q Exactive HF mass spectrometer (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2024Quote: ... The samples were desalted on Hypersep C-18 spin tips (Thermo Fisher Scientific, MA, USA) and the peptides dried under vacuum at low heat (Eppendorf ...
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bioRxiv - Immunology 2024Quote: ... We used an analytical column RSLC PepMan C-18 (Thermo Fisher Scientific, 2uM, 100Å, 75μm id x 50cm) at 55◦C with the mobile phase comprising buffer A (0.1% formic acid in water ...
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bioRxiv - Immunology 2024Quote: ... and quantified by fluorometric peptide assay kit (Thermo Fisher Scientific) prior to mass spectrometry analysis.
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bioRxiv - Immunology 2024Quote: ... connected to Q Exactive HF mass spectrometer (Thermo Fisher Scientific). We used an analytical column RSLC PepMan C-18 (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... The dried peptides were resuspended in 5% acetonitrile in 0.1% formic acid (Thermo Scientific) and quantified by fluorometric peptide assay kit (Thermo Fisher Scientific ...