Labshake search
Citations for Thermo Fisher :
4851 - 4900 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2024Quote: Larvae were paralyzed by a 1 minute immersion in α-bungarotoxin (20 µl of 1 mg/ml solution in system water; B1601, ThermoFisher Scientific), followed by washing in fresh system water (Duboué et al. ...
-
bioRxiv - Neuroscience 2024Quote: ... Conjugation and concentration of the purified solution was monitored through UV-Vis (Thermo Scientific, NanoDrop) measurement of absorbance at 260 nm (for detection of PNA ...
-
bioRxiv - Neuroscience 2024Quote: ... RNA for confirming RNA-seq findings was isolated from more than 10000 astrocytes sorted by FACS using the PicoPure RNA isolation kit (KIT0204, Life Technologies). cDNA was generated with SuperScript III First-Strand Synthesis SuperMix (11752250 ...
-
bioRxiv - Neuroscience 2024Quote: ... 10 kD Texas red-conjugated dextran (Molecular Probes) at 2.5 mM was injected into the soft tissue between two abdominal segments of the exoskeleton of flies with and without TBI ...
-
bioRxiv - Neuroscience 2024Quote: ... cDNA was generated with SuperScript III First-Strand Synthesis SuperMix (11752250, Invitrogen). For each gene ...
-
bioRxiv - Neuroscience 2024Quote: ... Concentration and integrity of cDNA was assessed using Qubit 3.0 Flurometer (Life Technologies) and Agilent 2100 Bioanalyzer (Agilent Technologies ...
-
bioRxiv - Neuroscience 2024Quote: ... All secondary antibodies (Alexa Fluor 488, 568 or 633 labeled; Molecular Probes) were used at a dilution of 1:1000 ...
-
bioRxiv - Neuroscience 2024Quote: ... The nuclei dye To-Pro-3 Iodide (T3605, ThermoFisher) was also used at a dilution of 1:1000 ...
-
bioRxiv - Neuroscience 2024Quote: ... The slices were then washed 3 x 5 min in 0.5% PBST followed by incubation in secondary antibodies (goat anti-mouse Alexa Fluor 405 1:500, Invitrogen A31553 ...
-
bioRxiv - Neuroscience 2024Quote: ... sections were incubated for 1h at 25°C in Alexa Fluor® 647 (Invitrogen) goat anti-rabbit secondary antibody (1:500 in 1% BSA-TBS).
-
bioRxiv - Neuroscience 2024Quote: Nanoparticles were targeted to the neuronal cell membrane via biotinylated A2B5 antibody (433110, Invitrogen). Neuronal cultures grown on a 12 mm diameter coverslip were loaded in the imaging chamber (ALA scientific) ...
-
bioRxiv - Neuroscience 2024Quote: ... 9:1 in 10x HBSS (Gibco/Thermo Fischer Scientific, U.S.) and 35% SIP was prepared by diluting 100% SIP 0.35:1 in 1x HBSS pH 7.0-7.4 ...
-
bioRxiv - Neuroscience 2024Quote: ... The slides were then washed for 30 min in PBS followed by 2h incubation with Alexa-488-conjugated donkey anti-rabbit secondary antisera (1:500; Invitrogen). Slides went through a final wash for 30 min in PBS and mounted with glycerol containing (2.5% DABCO ...
-
bioRxiv - Molecular Biology 2024Quote: ... Proteins were transferred to PVDF membrane using the Iblot 2 system (Thermo Fisher Scientific IB21001). Membranes were then blocked for 1-2 hours in 5% powdered milk (Amresco ...
-
bioRxiv - Molecular Biology 2024Quote: The Obox4 coding sequence was codon-optimized using GeneArt Instant Designer (Thermo Fisher), to remove the ASO target motif and improve translation efficiency ...
-
bioRxiv - Molecular Biology 2024Quote: ... and the amount of transcript (copies/microliter) was determined using the Analysis Suite (Thermo Fisher Scientific).
-
bioRxiv - Molecular Biology 2024Quote: ... and then polyadenylated using a poly(A) tailing kit (catalog no. AM1350, Thermo Fisher), according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2024Quote: ... with MOPS running buffer (Thermo Scientific NP0001). For whole cell lysates ...
-
bioRxiv - Molecular Biology 2024Quote: ... diluted in blocking buffer under a Hybrislip (Invitrogen: H-18202). Following primary antibody staining overnight ...
-
bioRxiv - Molecular Biology 2024Quote: ... designed on flanking and inside of the Obox4 cluster were synthesized using a Precision gRNA Synthesis Kit (catalog no. A29377, Thermo Fisher) according to the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2024Quote: ... or SuperSignal West Dura extended Duration Substrate (Fisher Scientific 37071). Hyblot CI autoradiography film (Thomas Scientific 1159M38 ...
-
bioRxiv - Molecular Biology 2024Quote: Samples were run on precast Tris-Glycine 8% Novex gels (Thermo Fisher Scientific XP00080BOX) with GTS running buffer (Tris 25 mM ...
-
bioRxiv - Molecular Biology 2024Quote: ... followed by chemiluminescence either with Peirce ECL western Blotting Substrate (Thermo Fisher Scientific 32106), SuperSignal West Pico Plus (Thermo Fisher 34580) ...
-
bioRxiv - Molecular Biology 2024Quote: ... secondary antibody and DAPI stain (Invitrogen, 1/10,000) diluted in PBS for 1 hour ...
-
bioRxiv - Molecular Biology 2024Quote: ... SuperSignal West Pico Plus (Thermo Fisher 34580), or SuperSignal West Dura extended Duration Substrate (Fisher Scientific 37071) ...
-
bioRxiv - Molecular Biology 2024Quote: ... with GTS running buffer (Tris 25 mM, Glycine 190 mM, SDS 0.1%) or 4-12% Bis-Tris precast NuPAGE gels (Thermo Fisher Scientific NP0322BOX) with MOPS running buffer (Thermo Scientific NP0001) ...
-
bioRxiv - Molecular Biology 2024Quote: ... Codon-optimized DNA was synthesized using Prime Gene Synthesis Services (Thermo Fisher). Codon-optimized Obox4 mRNA was transcribed in vitro using the mMESSAGE mMACHINE™ T7 Transcription Kit (catalog no ...
-
bioRxiv - Molecular Biology 2024Quote: ... Protein concentration was quantified using the Pierce BCA Protein Assay Kit (Thermo Scientific 23225). Lysates were mixed with equal volume 2x Laemmli buffer (Sodium Dodecyl Sulfate 4% ...
-
bioRxiv - Molecular Biology 2024Quote: ... and 10 µg/mL yeast tRNA (catalog no. AM7119, Thermo Fisher). After brief vortex and pulse spin ...
-
bioRxiv - Molecular Biology 2024Quote: ... Codon-optimized Obox4 mRNA was transcribed in vitro using the mMESSAGE mMACHINE™ T7 Transcription Kit (catalog no. AM1344, Thermo Fisher) and then polyadenylated using a poly(A ...
-
bioRxiv - Molecular Biology 2024Quote: ... RNase A (DNase and protease-free Thermo Fisher Scientific; #EN0531) was added along with the antibody to the plus RNase A samples at a final concentration of 50 µg/mL ...
-
bioRxiv - Molecular Biology 2024Quote: ... total RNA was purified according to the Trizol manufacturer’s instructions (ThermoFisher).
-
bioRxiv - Molecular Biology 2024Quote: ... and Proteinase K (ThermoFisher; #EO0491) treatments prior to DNA clean up (QIAGEN MinElute PCR Purification Kit Cat#28004) ...
-
bioRxiv - Molecular Biology 2024Quote: ... Following washes in RPE buffer RNA was eluted in RNase free water and analysed by electrophoresis on an agarose gel and quantified using the Qubit RNA High Sensitivity Assay Kit (ThermoFisher Q32852).
-
bioRxiv - Molecular Biology 2024Quote: ... 1.33μL of 1:4-diluted RT reaction was used in 5μL qPCR reactions with Taqman miRNA probes and Taqman Universal Mastermix II with UNG (ThermoFisher). Technical triplicates were run for each reaction on the Applied Biosystems QuantStudio Pro 6 ...
-
bioRxiv - Molecular Biology 2024Quote: ... Taqman miRNA probes (ThermoFisher), Taqman Universal Mastermix II with UNG (ThermoFisher) ...
-
bioRxiv - Molecular Biology 2024Quote: ... Alexa 568 (Invitrogen, 1:400), and Alexa 647 (Invitrogen ...
-
bioRxiv - Molecular Biology 2024Quote: ... 9.96ng of total RNA was used to perform 5μL reverse transcription reactions using the TaqMan MicroRNA Reverse Transcription kit (ThermoFisher). After RT ...
-
bioRxiv - Molecular Biology 2024Quote: RT-qPCR of isolated total RNA was performed using TaqMan miRNA assay reagent (Life Technologies). ΔΔCT value was calculated using Sn2841 and U18 snoRNA as endogenous controls.
-
bioRxiv - Molecular Biology 2024Quote: ... The eluted material was then subjected to RNase A (DNase and protease-free Thermo Fisher Scientific; #EN0531) and Proteinase K (ThermoFisher ...
-
bioRxiv - Neuroscience 2024Quote: ... Alexa Fluor 594-conjugated donkey anti-goat IgG (1:1,000; Cat# A-11058, RRID: AB_142540, Invitrogen; Thermo Fisher Scientific Inc., MA, USA), and AMCA AffiniPure donkey anti-mouse IgG (1:1,000 ...
-
bioRxiv - Neuroscience 2024Quote: ... The secondary antibodies used in this study were Alexa Fluor 488-conjugated goat anti-rabbit IgG (1:5,000; Cat# A-11008, RRID: AB_143165, Invitrogen; Thermo Fisher Scientific Inc., MA, USA), Alexa Fluor 488-conjugated donkey anti-rabbit IgG (1:5,000 ...
-
bioRxiv - Neuroscience 2024Quote: ... Samples were then washed three times with washing buffer (1×PBS containing 0.1% Tween-20) and incubated with the Alexa Fluor conjugated secondary antibodies (1:500, Thermo Fisher Scientific, Cat#A11039, 21206,10042) for 2 h at room temperature ...
-
bioRxiv - Neuroscience 2024Quote: ... Alexa Fluor 594-conjugated donkey anti-goat IgG (1:1,000; Cat# A-11058, RRID: AB_142540, Invitrogen; Thermo Fisher Scientific Inc. ...
-
bioRxiv - Neuroscience 2024Quote: ... The secondary antibodies used in this study were Alexa Fluor 488-conjugated goat anti-rabbit IgG (1:5,000; Cat# A-11008, RRID: AB_143165, Invitrogen; Thermo Fisher Scientific Inc. ...
-
bioRxiv - Molecular Biology 2024Quote: ... Dynabeads protein-G (Thermo Fisher Scientific, catalogue #10004D) was washed three times using cold lysis buffer and then incubated with rabbit polyclonal ALG-1 antibody in 250µl lysis buffer for 1 hr at room temperature with rotation ...
-
bioRxiv - Molecular Biology 2024Quote: ... IPed ALG-1 was incubated in a slicing buffer containing a radiolabelled miR-1 target RNA probe along with Yeast RNA (Ambion, catalogue #AM7120G) and RNAse inhibitor at 20°C for 90 minutes for slicing reaction ...
-
bioRxiv - Molecular Biology 2024Quote: ... Proteins were detected using a 1:10,000 dilution of fluorescent IRDye 800CW goat anti-mouse IgG (925-32210; Thermofisher) or IRDye 680RD Goat anti-Mouse IgG Secondary Antibody (925-68070 ...
-
bioRxiv - Molecular Biology 2024Quote: ... before hypoxia induction the media was changed to Normoxic (cortical neurons growth media with Neurobasal changed to Gibco A2477501 supplemented with D-Glucose 25mM and Sodium Pyruvate 0.2mM ...
-
bioRxiv - Molecular Biology 2024Quote: ... or OGD (cortical neurons growth media with Neurobasal changed to Gibco A2477501). Normoxic cells were then put back in the incubator ...