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Citations for Macherey-Nagel GmbH :
301 - 350 of 3058 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: RNA was isolated using the NucleoSpin RNA kit from Macherey-Nagel according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... all isolates were grown overnight at 20 °C in liquid Zobell medium and bacterial DNA was extracted using the Nucleospin tissue kit following the manufacturer’s instructions (Macherey-Nagel).
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bioRxiv - Plant Biology 2023Quote: The Protino® Ni-NTA column (Macherey-Nagel, Düren, Germany) was used for affinity chromatography of recombinant CAM2-His6 from E ...
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bioRxiv - Synthetic Biology 2023Quote: ... The plasmid library was finally purified by NucleoBond Midi-prep Kit (Macherey-Nagel #740410) and stored at –20°C before use ...
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bioRxiv - Synthetic Biology 2023Quote: ... The plasmid library was finally purified by NucleoBond Midi-prep Kit (Macherey-Nagel #740410) and stored at –20°C before use ...
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bioRxiv - Systems Biology 2023Quote: ... resuspended in sterilized water and filtered under vacuum using a 0.2 µm pore size filter (predried and preweighted; PORAFIL® MV; Macherey-Nagel). The filter was once more dried (overnight 12-24 h in an oven at 60⁰C ...
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bioRxiv - Synthetic Biology 2023Quote: ... genomic DNA was first extracted from 1 x 105 cells using NucleoSpin Tissue (MACHEREY-NAGEL #740952) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2023Quote: ... Protino® Glutathione Agarose 4B (Macherey-Nagel) was used ...
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bioRxiv - Biophysics 2023Quote: ... Clean up of the PCR products was done using a standard cleanup protocol (NucleoSpin Gel and PCR cleanup, Macherey-Nagel GmbH). 2 ...
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bioRxiv - Biophysics 2023Quote: ... The 600 bp DNA was extracted from the gel using a standard NucleoSpin Gel and PCR cleanup (Macherey-Nagel GmbH). The fluorescence of the doubly labelled 600 bp band was visualized using a Typhoon 9400 scanner (GE Healthcare ...
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bioRxiv - Neuroscience 2023Quote: ... using the PCR and gel clean-up kit (Macherey-Nagel) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... according to the manufacturer’s protocol (Macherey-Nagel). Sanger sequencing was carried out on purified plasmid vector to validate the sgRNA incorporation ...
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bioRxiv - Systems Biology 2023Quote: ... cDNA was generated by isolating RNA from 5×106 HEK293T cells with the Nucleospin RNA isolation Mini kit (Macherey-Nagel)according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2023Quote: RNA was extracted according to the manufacturer’s instructions (NucleoSpin® RNA, MACHEREY-NAGEL) and measured by NanoPhotometer®N60 (IMPLEN) ...
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bioRxiv - Physiology 2023Quote: ... Tissues were harvested and directly transferred into RNA stabilization solution (NucleoProtect® RNA, MACHEREY-NAGEL) overnight at 4 degrees and then stored in -80 degrees ...
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bioRxiv - Plant Biology 2023Quote: ... Separation was carried out using a reversed-phase column (NUCLEODUR C18 Pyramid, EC 50/2, 50×2 mm, 1.8 μm, Macherey-Nagel, Düren, Germany), using an ekspert ultraLC 100 UHPLC system coupled to a QTRAP 4500 mass spectrometer (AB SCIEX ...
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bioRxiv - Cell Biology 2023Quote: ... and purified with a silica-based resin column (MACHEREY-NAGEL, protino Ni-IDA). Aliquots were flash frozen in liquid nitrogen and stored at –80°C ...
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bioRxiv - Immunology 2023Quote: ... and ADSCs were lysed in the plate using in lysis buffer containing TCEP (Macherey-Nagel). Lysed cells were snap frozen in nitrogen and stored at -80°C until use.
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bioRxiv - Immunology 2023Quote: ... and neutrophils were harvested by gentle pipetting to asses activation status and phosphorylation of STAT3 or lysed in lysis buffer containing TCEP (Macherey-Nagel) for RNA sequencing ...
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bioRxiv - Cell Biology 2023Quote: ... Total RNA was isolated with the NucleoSpin RNA kit (Macherey-Nagel, Düren, Germany) as instructed by the manufacturer ...
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bioRxiv - Cancer Biology 2023Quote: Total RNA extraction was performed using NucleoSpin RNA Kit (Macherey-Nagel) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Equilibrated Protino Ni-IDA beads (Macherey-Nagel) were added and rotated for 30 min ...
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bioRxiv - Molecular Biology 2023Quote: Total RNA was purified with the NucleoSpin RNA Clean-up (Macherey-Nagel) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: RNA was extracted using the Macherey-Nagel™ Mini kit Nucleospin™ (Macherey-Nagel, # 872061) or Qiagen RNeasy micro kit (Qiagen ...
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bioRxiv - Molecular Biology 2023Quote: ... The cells were then treated with RNase (Macherey-Nagel, Thermo Fisher Scientific), stained with propidium iodide (PI ...
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bioRxiv - Molecular Biology 2023Quote: Total DNA was extracted from 1 × 106 cells using NucleoSpin DNA RapidLyse (MACHEREY-NAGEL). qPCR was performed with primers targeting the ND2 locus in the mitochondrial genome (5′-TGTTGGTTATACCCTTCCCGTACTA-3′ and 5′- CCTGCAAAGATGGTAGAGTAGATGA-3′ ...
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bioRxiv - Molecular Biology 2023Quote: ... the beads were resuspended in NTI buffer for DNA purification using NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel, #740609.250).
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bioRxiv - Microbiology 2023Quote: ... 50 μl of a 40% slurry Ni-NTA agarose beads (20 μl beads, Macherey-Nagel) were added followed by an incubation at 4°C for 45 min with shaking ...
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bioRxiv - Microbiology 2023Quote: ... Its DNA was extracted from mycelium and conidia with the NucleoBond High Molecular Weight DNA kit (Macherey-Nagel), with the mechanical disruption of about 30 mg of lyophilized mycelium with beads for 5 min at 30 Hz ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA was purified using the NucleoSpin Gel and PCR Clean-up (#74609.250, Macherey-Nagel, Düren, Germany) kit with NTB binding buffer (#740595.150 ...
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bioRxiv - Microbiology 2023Quote: ... DNA was purified using a MaxiPrep Kit (Macherey-Nagel). Plasmid stocks were deep-sequenced to ensure complete representation.
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bioRxiv - Microbiology 2023Quote: ... The pBAC infectious clone plasmid was extracted and purified using NucleoBond Xtra Maxi prep kit (Macherey-Nagel). The plasmid was then sequenced using Primordium Labs “Large” whole plasmid sequencing service ...
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bioRxiv - Microbiology 2023Quote: ... DNA samples were extracted using the genomic DNA NucleoMag tissue kit from Macherey-Nagel (Hoerdt, France). DNA libraries were prepared and indexed ...
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bioRxiv - Microbiology 2023Quote: ... Pooled libraries were concentrated by ethanol precipitation and purified by gel extraction of the corresponding library size using the NucleoSpin Gel and PCR Clean-up Mini kit (Macherey-Nagel). Libraries were sequenced using the MiSeq Reagent Kit v3 with 300-bp paired end reads on a MiSeq (Illumina).
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bioRxiv - Microbiology 2023Quote: 3D-PCR products were purified from agarose gels (NucleoSpin Gel and PCR Clean-up, Macherey-Nagel) and ligated into the TOPO TA cloning vector (Invitrogen) ...
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bioRxiv - Microbiology 2023Quote: ... PCR products were purified from agarose gels (NucleoSpin Gel and PCR Clean-up, Macherey-Nagel). Nested PCR (position ...
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bioRxiv - Microbiology 2023Quote: ... or Protino Ni-IDA 2000 packed columns (Macherey-Nagel, Düren, Germany). Protein concentrations were measured on a Qubit 2.0 (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... Δ50Procm was purified on Protino Ni-IDA 2000 Packed Columns (Macherey-Nagel, Düren, Germany) and elution fractions containing Δ50Procm were dialyzed in a 6–8 kDa dialysis membrane (Spectrum Chemical ...
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bioRxiv - Microbiology 2023Quote: ... or Protino Ni-IDA 2000 packed columns (Macherey-Nagel, Düren, Germany) for Δ50Pro and Δ50Pro-E/A-VPg to facilitate the removal of the proteolytically cleaved N-terminal region of Pro ...
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bioRxiv - Microbiology 2023Quote: Genomic DNA of all mcr-positive isolates was extracted using NucleoSpin® Tissue kit (Macherey-Nagel GmbH & Co, Duren, Germany). The libraries were prepared using Nextera XT DNA Sample Preparation Kit and sequenced on MiSeq or NovaSeq 6000 platform (Illumina ...
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bioRxiv - Microbiology 2023Quote: ... and NucleoSpin Virus (Macherey-Nagel, Düren, Germany). DNA was quantified and the 260 nm/280 nm ratio was determined using a NanoDrop ND-1000 (Thermo Fisher Scientific Inc. ...
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bioRxiv - Microbiology 2023Quote: ... The final products were purified by gel extraction from a 1% gel with the NucleoSpin Gel and PCR clean-up kit (Macherey-Nagel) and verified by Sanger sequencing.
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bioRxiv - Microbiology 2023Quote: ... The final products were purified by gel extraction from a 1% gel with the NucleoSpin Gel and PCR clean-up kit (Macherey-Nagel) and sent for Sanger sequencing.
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bioRxiv - Evolutionary Biology 2023Quote: ... The resulting powder was used for DNA extraction using the NucleoSpin® Tissue kit following manufacturer instructions (MACHEREY-NAGEL GmbH & Co. KG). DNA quantity and purity were checked with a Nanodrop One spectrophotometer (Thermo Scientific ...
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bioRxiv - Systems Biology 2023Quote: ... samples were lysed and isolated using the vendor’s instructions of NucleoSpin RNA plus (Macherey-Nagel) with the addition of a DNase removal step using RNase-Free Dnase (Qiagen) ...
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bioRxiv - Microbiology 2023Quote: ... DNA fragments used in cloning reactions were purified using the NucleoSpin gel and PCR cleanup kit (Macherey-Nagel) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Plasmid DNA isolations were performed using the NucleoSpin Plasmid kit (Macherey-Nagel) according to the manufacturer’s instructions.
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bioRxiv - Genetics 2023Quote: ... using the RNA-Plus kit (Macherey-Nagel), and libraries were prepared with 1μg of total RNA using the NEBNext® Ultra™ II RNA Library Prep Kit for Illumina® according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... DNA was extracted using the NucleoMag Blood 200 μL kit (Macherey-Nagel).
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bioRxiv - Genomics 2023Quote: ... Amplification products were cleaned up using the Nucleospin Gel and PCR clean up (Macherey-Nagel, Germany). Ten libraries with unique multiplex identifier linkers (MID ...