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Citations for Macherey-Nagel GmbH :
451 - 500 of 3300 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... DNA was extracted using a NucleoSpin Microbial DNA Kit (Macherey-Nagel). The 16S rRNA gene was amplified by PCR using the universal bacterial primers 8F and 1391R (23) ...
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bioRxiv - Immunology 2024Quote: ... After purification of the RT-PCR products using the NucleoSpin Gel and PCR clean-up kit (Macherey-Nagel) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... All Minipreps and Midipreps were performed using endotoxin-free kits (Macherey-Nagel).
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bioRxiv - Immunology 2024Quote: Total RNA was extracted from LECs using the NucleoSpin RNA Kit (Macherey-Nagel), and library preparation was performed according to the TruSeq Stranded mRNA Sample Preparation protocol using the TruSeq Stranded mRNA HT Kit (Illumina) ...
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bioRxiv - Immunology 2024Quote: RNA was extracted using the NucleoSpin RNA Kit (Macherey-Nagel, Düren, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... and concentrated by NucleoSpin Gel and PCR Clean-up kit (MACHEREY-NAGEL). The quality and quantity of the DNA fragments were assessed on Agilent 2200 TapeStation (Agilent Technologies) ...
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bioRxiv - Genomics 2024Quote: ... and purified via gel extraction (MACHEREY-NAGEL) to enable targeted insertion ...
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bioRxiv - Genomics 2024Quote: ... The PCR product was purified using the NucleoSpin Gel and PCR Clean-up Kit (MACHEREY-NAGEL), following the manufacturer’s protocol ...
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bioRxiv - Genomics 2024Quote: TempO-LINC libraries were purified using the NucleoSpin Gel and PCR Clean-up Kit (Macherey-Nagel, cat# 740609.250), according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... SLS was precipitated by acidification and peptides were desalted by using C18 solid phase extraction cartridges (Macherey-Nagel). Cartridges were prepared for sample loading by adding acetonitrile (ACN) ...
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bioRxiv - Immunology 2024Quote: Total RNA was extracted using the total RNA and protein isolation kit (Macherey-Nagel). RNA samples were reverse-transcribed with High-Capacity cDNA Reverse Transcription reagents kit (Applied Biosystems ...
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bioRxiv - Immunology 2024Quote: ... We then stored the pellet at -20 °C for RNA isolation using the NucleoSpin RNA kit (MACHEREY-NAGEL, Düren, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... DNA was extracted using the Nucleomag plant DNA kit (Macherey-Nagel, Düren, Germany), a CTAB-based extraction protocol ...
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bioRxiv - Genomics 2024Quote: ... Purified DNA for the Vitis vinifera high-coverage sample and Actinidia melanandra were extracted using the Nucleobond High Molecular Weight DNA kit (Macherey-Nagel, Düren, Germany). Nuclei isolation was performed prior to DNA extraction for the Actinidia sample using the PacBio protocol (Pacific Biosciences ...
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bioRxiv - Immunology 2024Quote: ... Plasmids were isolated using the Nucleo Spin Plasmid Quick Pure protocol (Macherey-Nagel) and PCR purified using the Nucleo Spin Gel and PCR Clean-up Kit (Macherey-Nagel ...
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bioRxiv - Immunology 2024Quote: ... the band of correct size was excised under 70% UV light using a clean scalpel and DNA was extracted using Nucleo Spin Gel and PCR Clean-up Kit (Macherey-Nagel). DNA sequences were confirmed by sequencing ...
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bioRxiv - Immunology 2024Quote: ... Cells were cultivated in LB medium containing 50 µg/ml Kanamycin and plasmids were isolated using the Nucleo Spin Plasmid Quick Pure protocol (Macherey-Nagel).
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bioRxiv - Immunology 2024Quote: ... and PCR purified using the Nucleo Spin Gel and PCR Clean-up Kit (Macherey-Nagel) before microinjection.
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bioRxiv - Genomics 2024Quote: Foot tissue was gently dissolved by addition of 1 µL of 50 µg/mL Proteinase K (Macherey-Nagel GmbH & Co. KG, Duren, Germany) to 1 ml TNE8U buffer containing the tissue and incubating at 55°C overnight ...
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bioRxiv - Immunology 2024Quote: ... organs were homogenized at 4 °C using Bead Tubes type F (Macherey-Nagel) and the lysate was further used for RNA isolation ...
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bioRxiv - Microbiology 2024Quote: ... We made pH measurements by thawing the 18 microbiome-only microcosms and using the pH-Fix 2.0-9.0 test-strips (Macherey-Nagel). From the same microcosms we prepared supernatant ...
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bioRxiv - Microbiology 2024Quote: ... PCR product fragments were excised from gels and purified with a PCR clean-up kit (Macherey-Nagel Nucleospin).
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bioRxiv - Microbiology 2024Quote: ... The amplified inserts were purified using NucleoSpin® Gel and PCR Clean-up (MACHEREY-NAGEL). For the ligation assay ...
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bioRxiv - Microbiology 2024Quote: ... Total DNA was extracted from the swab samples using the NucleoSpin® 96 Soil (Macherey-Nagel) kit ...
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bioRxiv - Microbiology 2024Quote: ... The organic fractions were dried and redissolved in 40 µL CHCl3/methanol (80:20) prior to analysis by thin layer chromatography on 0.2 mm silica plates (Macherey-Nagel Xtra SIL G) developed with CHCl3/methanol/acetic acid (80:15:8 ...
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bioRxiv - Plant Biology 2024Quote: ... Tissues were ground with mortar and pestle using liquid nitrogen prior total RNAs extraction using the RNA Plant and Fungi NucleoSpin™ (Macherey-Nagel™). DNA was eliminated using the Macherey-Nagel™ Kit rDNase ...
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bioRxiv - Microbiology 2024Quote: RNA was isolated from the superior lobe of the lung with a NucleoSpin® RNA kit (Macherey-Nagel) and cDNA was prepared with the use of SuperScript™ II Reverse Transcriptase (Invitrogen ...
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bioRxiv - Microbiology 2024Quote: ... (ThermoFischer) was prepared using the NucleoBond XTRA Midi kit (Macherey-Nagel # 740410.50) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2024Quote: ... C3 and C5 clones using NucleoSpin Blood QuickPure kit (Macherey-Nagel GmbH, Düren, Germany). The library for MinION was constructed with a Ligation Sequencing Kit ...
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bioRxiv - Microbiology 2024Quote: DNA samples extracted from mouse fecal pellets using the NucleoSpin Soil DNA Extraction Kit (Macherey-Nagel, Germany), underwent 16S rRNA gene amplicon sequencing ...
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bioRxiv - Microbiology 2024Quote: ... and stored in endotoxin-free H2O at −20°C (Macherey-Nagel).
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bioRxiv - Microbiology 2024Quote: The PCR product was run on a 0.8% agarose gel and a fragment of the correct size was excised for clean up using a NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel, Ref. No. 740609.5) followed by Ampure XP beads (Beckman Coulter ...
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bioRxiv - Microbiology 2024Quote: ... PCR samples were purified using the NucleoSpin® Gel and PCR Clean-up kit (Macherey-Nagel, France), and sent for DNA sequencing (Eurofins ...
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bioRxiv - Synthetic Biology 2024Quote: ... Amplicons were then purified using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel GmbH, Cat. # 740609.50) and sequenced through Sanger sequencing (Microsynth Seqlab GmbH).
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bioRxiv - Synthetic Biology 2024Quote: ... The PCR product was purified using NucleoSpin Gel and PCR Clean-up Kit (Macherey-Nagel, Cat. #740609) and was used as a template in KAPA HiFi HotStart ReadyMix (Roche Diagnostics ...
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bioRxiv - Molecular Biology 2024Quote: ... Aliquots (4 - 6 mL) of overnight cultures were used for recipient plasmid extraction using the NucleoSpin plasmid kit (Macherey-Nagel).
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bioRxiv - Plant Biology 2024Quote: ... Lipid classes were separated using an Agilent 1260 Infinity II HPLC system using a 150 mm×3 mm (length × internal diameter) 5 µm diol column (Macherey-Nagel), at 40°C ...
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bioRxiv - Microbiology 2024Quote: ... This was then gel purified using a purification kit (Macherey-Nagel 740609) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... The PCR products were cleaned using a Nucleospin Gel and PCR Clean-up kit (Macherey-Nagel 740609.250) then cloned into the pCRII vector using a TA Cloning Kit Dual Promoter pCRII Vector (Invitrogen K207020 ...
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bioRxiv - Microbiology 2024Quote: ... The gRNA cassette was isolated by gel electrophoresis with the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel), and the DNA was then amplified by PCR using primers with Illumina sequencing adapters ...
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bioRxiv - Microbiology 2024Quote: ... Genomic DNA was isolated using NucleoSpin Blood kits (Macherey-Nagel) and digested for 18 hours with SbfI-HF (NEB) ...
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bioRxiv - Genomics 2024Quote: ... NucleoMag™ magnetic separation beads (Macherey-Nagel, Düren, Germany) were used for double-ended size selection targeting an insert size of 300 bp ...
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bioRxiv - Immunology 2024Quote: ... Mini kit for DNA from cells and tissue (Macherey-Nagel) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... and RNA was isolated using a Mini Kit for RNA and protein purification (NucleoSpin RNA/Protein, Macherey-Nagel) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2024Quote: ... and then gel purified using NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel, Germany). The plasmid backbone ...
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bioRxiv - Synthetic Biology 2024Quote: ... and purified using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel GmbH, Cat. # 740609.50). Plasmid sequences were verified either by full plasmid sequencing (Plasmidsaurus Inc ...
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bioRxiv - Synthetic Biology 2024Quote: ... The resulting PCR product was purified using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel GmbH, Cat. # 740609.50), following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... The supernatant was cleared by centrifugation at 18,000 rpm and applied to a Ni-IDA resin (Macherey-Nagel). The resin was washed with lysis buffer and the protein was eluted with lysis buffer supplemented with 250 mM imidazole ...
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bioRxiv - Microbiology 2024Quote: ZIKV RNA was isolated from mouse serum using Nucelospin RNA virus kit (Macherey-Nagel, Düren, Germany), and cDNA was synthesized using ProtoScript II Reverse Transcriptase (New England Biolabs ...
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bioRxiv - Immunology 2024Quote: Serum samples from all experimental groups were extracted using the NucleoSpin RNA isolation kit (Macherey-Nagel, Allentown, PA). Following viral RNA extraction ...