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Citations for Macherey-Nagel GmbH :
451 - 500 of 3263 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2024Quote: The tRNA fraction was isolated from 17 µg of total RNA using Nucleobond AX-20 columns (Macherey-Nagel) following a rescaled protocol based on (28) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and NucleoSpin RNA (Macherey-Nagel, Düren, Germany). Extracted RNA was quantified using a Nanodrop ND 1000 spectrophotometer (Thermo Fisher Scientific).
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Braf-mutant Schwann cells divert to a repair phenotype to induce congenital demyelinating neuropathybioRxiv - Developmental Biology 2024Quote: ... total RNA was extracted using the NucleoSpin RNA Plus S kit (#740984; Macherey-Nagel). RNA concentrations were measured on a NanoDrop (NanoDrop TM 1000 Spectrophotometer ...
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bioRxiv - Genetics 2024Quote: Plasmid DNA for sequencing was extracted using a plasmid kit (Macherey-Nagel NucleoSpin Plasmid mini) as per the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... Cells were washed twice and RNA extraction and purification were performed using NucleoSpin® RNA plus (Macherey-Nagel, 740984.250) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... RNA of sorted cells was isolated by using NucleoSpin RNA XS (Macherey-Nagel) and 20 ng carrier RNA ...
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bioRxiv - Cancer Biology 2024Quote: ... the 28S and 18S bands were cut from the gel and RNA was extracted from agarose using Nucleospin Gel and PCR clean-up (Macherey-Nagel) columns.
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bioRxiv - Cancer Biology 2024Quote: ... and the resulting fragment was purified using the NucleoSpin Gel and PCR Clean-up kit according to the manufacturer’s instructions (Macherey-Nagel, 740609.250). The purified CASP3 gene fragment was ligated into the linearized pEGFP-N3 vector using the quick ligationTM kit according to the manufacturer’s instructions (NEB ...
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bioRxiv - Microbiology 2024Quote: ... Cells were harvested and genomic DNA was isolated using NucleoSpin Microbial DNA Mini kit for DNA (Macherey-Nagel). Bacterial genomes were sequenced at Plasmidsaurus (https://www.plasmidsaurus.com/ ...
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bioRxiv - Cancer Biology 2024Quote: ... and the plasmid was amplified and purified using the Nucleobond Xtra Midi kit (Macherey-Nagel, 740410.50).
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bioRxiv - Cancer Biology 2024Quote: ... and the resulting digested fragment was purified using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel, 740609.250). Guide RNAs were designed using the CRISPOR tool (http://crispor.gi.ucsc.edu/ ...
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bioRxiv - Biochemistry 2024Quote: ... and NucleoBond Xtra Midi kit (MACHEREY-NAGEL, 740410.50).
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bioRxiv - Cell Biology 2024Quote: ... Cells were harvested by scraping in RA1 lysis buffer and total RNA was isolated using the NucleoSpin RNA Mini Kit for RNA Isolation (Macherey-Nagel, 740955) according to manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... gDNA was isolated using the NucleoSpin® gDNA Clean-up Kit from Macherey-Nagel. Isolation was performed according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: Total RNA was extracted using the Nucleospin® RNA II kit (Macherey-Nagel, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... Column chromatography was carried out under positive pressure using 15-40 µm silica gel (Macherey-Nagel) and the indicated solvents ...
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bioRxiv - Cancer Biology 2024Quote: ... and plasmid DNA was purified with NucleoSpin Plasmid Mini kit (Macherey-Nagel, Düren, Germany) according to the manufacturer instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... Reactions were monitored by analytical thin layer chromatography (TLC) carried out on ALUGRAM Xtra SIL G/UV254 silica gel plates (Macherey-Nagel). Compounds were visualized with a UV lamp (λ = 254 nm ...
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bioRxiv - Cell Biology 2024Quote: ... using the NucleoSpin RNA kit (Macherey-Nagel, Cat no. 740955.250) according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2024Quote: ... the next day plasmid DNA was extracted (Nucleospin Plasmid, Macherey-Nagel) and sent for Sanger sequencing (Eurofins Genomics) ...
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bioRxiv - Genetics 2024Quote: ... bands were cut from the gel and extracted using column purification (Macherey-Nagel, REF-740609.250). Samples were submitted for sequencing at Azenta/Genewiz to confirm gene deletions or fragment insertions.
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bioRxiv - Synthetic Biology 2024Quote: ... the genomic DNA of the expanded clones was extracted with a kit according to the manufacturer’s protocol (Nucleospin Tissue, Macherey-Nagel) to perform PCR genotyping ...
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bioRxiv - Systems Biology 2024Quote: ... The sgRNA library plasmid DNA was extracted by using NucleoBond Xtra Midi kit (Macherey-Nagel).
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bioRxiv - Molecular Biology 2024Quote: ... The reverse transcription products were purified by NucleoSpin Gel and PCR clean-up kit equilibrated with NTC buffer (Macherey-Nagel). Dideoxy DNA sequencing samples were prepared using Thermo Sequenase DNA polymerase (Cytiva ...
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bioRxiv - Synthetic Biology 2024Quote: ... All amplified DNA fragments were purified using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel), according to the vendor’s instructions ...
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bioRxiv - Plant Biology 2024Quote: RNA extraction utilized the Nucleospin RNA kit from Macherey-Nagel. Subsequently ...
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bioRxiv - Microbiology 2024Quote: ... Peptides were desalted by using C18 solid phase extraction cartridges (Macherey-Nagel). Cartridges were prepared by adding acetonitrile (ACN) ...
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bioRxiv - Microbiology 2024Quote: Total RNA from lung tissue cells for hamsters was isolated using NucleoSpin RNA Kit (740955.250, MACHEREY-NAGEL, Duren, Germany). cDNA libraries were prepared by KAPA mRNA HyperPrep Kit following the standard protocol ...
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bioRxiv - Neuroscience 2024Quote: ... Modified BAC DNA was prepared using the large construct DNA purification kit - NucleoBond® Xtra BAC (Macherey-Nagel), linearized with PmeI and purified through a Sepharose™ separation column68 ...
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bioRxiv - Neuroscience 2024Quote: ... BAC DNA for pronuclear injection was prepared using the large construct DNA purification kit - NucleoBond® Xtra BAC (Macherey-Nagel), linearized with PI-SceI and purified over a home-made Sepharose CL4B column (Johansson et al. ...
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bioRxiv - Microbiology 2024Quote: RNA was extracted from each strain cultivated in 7H9sB at 37°C for two days using Nucleospin kit (Macherey-Nagel, Allentown, PA) following manufacturer instructions with few modifications ...
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β-1,6-glucan plays a central role in the structure and remodeling of the bilaminate fungal cell wallbioRxiv - Microbiology 2024Quote: ... All PCR products were cleaned-up and concentrated with NucleoSpin Gel and PCR Clean-up (Macherey-Nagel). PCR products (3 µg of CaCAS9 cassette ...
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bioRxiv - Microbiology 2024Quote: ... DNA was routinely purified with the NucleoSpin gel and PCR clean-up kit (Macherey-Nagel) according to the manufacturer’s instruction or by ethanol precipitation ...
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bioRxiv - Microbiology 2024Quote: HBV RNAs and DNA were extracted from cells with the Nucleospin RNA (Macherey-Nagel) and MasterPureTM Complexe Purification Kit (BioSearch Technologies ...
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bioRxiv - Microbiology 2024Quote: ... plasmids were prepared using the Nucleobond Xtra Midi Endotoxin Free DNA preparation kit (Macherey-Nagel).
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bioRxiv - Microbiology 2024Quote: ... the lysate was treated with 2 µg/µl (w/v) RNAse A (Macherey-Nagel). Genomic DNA of L ...
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bioRxiv - Microbiology 2024Quote: The genomic ssDNA was isolated from the ion exchange-purified sample of the phage using a Virus DNA kit (Macherey-Nagel). For sequencing on the Oxford Nanopore platform ...
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bioRxiv - Immunology 2024Quote: Total RNA was extracted from cells at the indicated time points post stimulation and/or infection using the NucleoSpin RNA extraction kit (Macherey-Nagel) as directed by manufacturer ...
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bioRxiv - Microbiology 2024Quote: ... Single colonies were grown in 5 ml liquid LB medium at 37 °C overnight and the plasmid DNA was extracted using NucleoSpin® Plasmid preparation kit according to the manufacturer’s instructions (Macherey-Nagel). Positive clones were identified by restriction digestion and DNA sequencing ...
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bioRxiv - Molecular Biology 2024Quote: ... Analyses were performed using a Varian HPLC system equipped with a NUCLEODUR SiOH column (4.6 mm x250 mm) (Macherey-Nagel) and a Prostar 330 diode array detector ...
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bioRxiv - Neuroscience 2024Quote: ... Porablot NCP nitrocellulose membranes (Macherey-Nagel, 741280) and Bio-Dot® SF filter paper (Bio-Rad ...
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bioRxiv - Microbiology 2024Quote: ... following the manufacturer’s instruction (Macherey-Nagel, Bethlehem, PA). 5′ RACE was carried out using SMARTer RACE 5′/3′ Kit (Takara Bio USA ...
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bioRxiv - Microbiology 2024Quote: ... The last purification step was performed with the RNA Nucleospin Kit according to manufacturer’s instructions (Macherey-Nagel). The integrity of the RNAs was verified using the Agilent 2100 bioanalyzer with the RNA 6000 Nano kit (Agilent Technologies) ...
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bioRxiv - Microbiology 2024Quote: ... RNA was extracted using NucleoSpin RNA (Macherey-Nagel, Düren, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Systems Biology 2024Quote: ... PCR products were purified with PCR clean up kit from Macherey-Nagel. Equal molar concentrations of PCR product were mixed and 5 µL of mix was added to 15 µL of Gibson Master mix ...
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bioRxiv - Systems Biology 2024Quote: ... PCR products were purified with PCR clean up kit from Macherey-Nagel. Equal molar concentrations of PCR product were mixed (one from either pMD019 or pMD024 and one from pCRRNA ...
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bioRxiv - Plant Biology 2024Quote: RNA was extracted from 50 mg of frozen and fine ground rosette leaf material from four biological replicates per genotype and growth condition using the NucleoSpin RNA Plant Kit (Macherey-Nagel, Düren, Germany), according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2024Quote: All proteins were purified using Ni-IDA (Macherey-Nagel) following the instructions provided by the manufacturer ...
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bioRxiv - Biochemistry 2024Quote: ... After cell lysis using a French press the cell debris was removed by centrifugation at 17000 g for 1 h at 4 °C and the cleared lysate was incubated with Protino IDA resin (Macherey-Nagel) for 20 min at 4° C ...
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bioRxiv - Cancer Biology 2024Quote: ... 1000 ng of total RNAs were isolated using the Nucleospin RNA kit (Macherey-Nagel, 740955) following manufacturer’s instructions ...