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Citations for Macherey-Nagel GmbH :
251 - 300 of 3058 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... Total RNA was isolated using the NucleoSpin RNA Mini kit (Macherey-Nagel) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... Plasmid DNA was isolated via the endotoxin-free NucleoBond Xtra Midi Plus EF kit (Macherey-Nagel). For transfection ...
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bioRxiv - Biochemistry 2024Quote: ... and purified using NucleoBond Xtra Midi kit (Macherey-Nagel GmbH, Duren, Germany). Directed site mutagenesis was made by substituting three amino acids in the ZapD sequence by Alanine (R20 ...
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bioRxiv - Biochemistry 2024Quote: ... Expressed proteins were captured on pre-packed Ni-IDA (Protino Ni-IDA Resin, Macherey-Nagel, Duren, Germany) columns ...
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bioRxiv - Developmental Biology 2024Quote: ... PCR products were run on gel electrophoresis before gel extraction of bands of interest (with sizes corresponding to either a wild-type allele or a deleted allele) (Nucleospin gel and PCR clean-up, Macherey-Nagel Cat# 740609.50). Fragments were cloned into blunt-end TOPO vector (Invitrogen Cat# 45024) ...
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bioRxiv - Microbiology 2024Quote: ... DNA was extracted using a Nucleospin PCR cleanup kit (Macherey-Nagel) and resuspended in 50 μl elution buffer (5 mM Tris-HCl [pH 8.5]) ...
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bioRxiv - Microbiology 2024Quote: ... Genomic DNA was then extracted following the Nucleospin Tissue Kit protocol (Macherey-Nagel) and resuspended in 50 μl elution buffer (5 mM Tris-HCl [pH 8.5]) ...
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bioRxiv - Microbiology 2024Quote: ... RNA was extracted using the RNA XS kit (Macherey-Nagel, Düren, Germany) from MS4 Ciona juveniles that underwent gut colonization ...
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bioRxiv - Cancer Biology 2024Quote: ... The resulting amplicons were purified using the NucleoSpin gel and PCR cleanup kit following the manufacturer’s instructions (Macherey-Nagel Inc., Bethlehem, PA) and submitted for Sanger sequencing with the same primers used to generate the amplicons ...
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bioRxiv - Developmental Biology 2024Quote: ... and resistant clones then used for miniprep plasmid preparations (Nucleospin plasmid, Macherey-Nagel, Cat # 740588.50), followed by sequencing ...
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bioRxiv - Immunology 2024Quote: Total RNA was prepared using RNA purification kit (Macherey-Nagel GmbH & Co. KG, Germany), followed by cDNA synthesis (Bio-line ...
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bioRxiv - Immunology 2024Quote: ... DNA was purified with NucleoSpin™ gDNA Clean-up Kit (Macherey-Nagel GmbH & Co. KG, Germany). For ChIP-qPCR assays ...
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bioRxiv - Cancer Biology 2024Quote: ... The fixed cells were washed twice with PBS and stained with 20 µg/mL propidium iodide (PI) containing 100 mg/mL RNAse A (740505, MACHEREY-NAGEL) for 15 min at room temperature ...
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bioRxiv - Zoology 2024Quote: ... The purified plasmid was prepared using the NucleoBond® Xtra Midi kit (MACHEREY-NAGEL, Düren, Germany) or the Genopure Plasmid Midi kit (Roche Diagnostics GmbH ...
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bioRxiv - Cancer Biology 2024Quote: ... RNA was isolated from Eµ-Myc lymphoma cells using a NucleoSpin RNA kit (Macherey-Nagel) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... monocytes and hMDM using the NucleoSpin® RNA kit (Macherey-Nagel®) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... RNA extraction (Macherey-Nagel RNA extraction kit; Germany) was done according to supplier instructions ...
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bioRxiv - Plant Biology 2024Quote: ... Lipid classes were separated using an Agilent 1200 HPLC system using a 150 mm × 3 mm (length × internal diameter) 5 µm diol column (Macherey-Nagel), at 40 °C ...
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bioRxiv - Microbiology 2024Quote: ... Cells were lysed mechanically via bead-beating and DNA was purified on columns (Macherey-Nagel, Germany). A robotized platform (Biomek4000 ...
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bioRxiv - Microbiology 2024Quote: ... the Genomic DNA from Microorganisms Kit (Macherey-Nagel, Germany) was used as per the manufacturer’s instructions ...
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bioRxiv - Biophysics 2024Quote: ... NucleoSpin® plasmid isolation kit and NucleoSpin® gel and PCR clean-up kit were purchased from Macherey-Nagel (Germany). PierceTM protease inhibitor tablets EDTA free and Pierce™ high capacity Ni-IMAC resin were purchased from Thermo Scientific (international) ...
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bioRxiv - Molecular Biology 2024Quote: ... were extracted using the NucleoSpin Soil kit (Macherey-Nagel) with a final elution volume of 50 µl following the manufacturer instructions ...
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bioRxiv - Molecular Biology 2023Quote: DNA extraction from dried filters was done using NucleoSpin™ Forensic Filter kit (Macherey-Nagel GmbH & Co .KG, Düren – Germany). Filter parts were folded and incubated in 600 μL of lysis buffer T1 at 56 °C for two hours with tube horizontally agitated and then centrifugated 1 min 30 sec at 11,000 g ...
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bioRxiv - Molecular Biology 2023Quote: ... using the NucleoSpin Blood Kit (Macherey-Nagel; Dueren, DE) following the manufacturer’s protocol and used as template in diagnostic PCR ...
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bioRxiv - Molecular Biology 2023Quote: ... Genomic DNA was extracted using a NucleoSpin Blood kit (Macherey-Nagel) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... and purified using the “PCR Clean-up System” (Macherey-Nagel).
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bioRxiv - Molecular Biology 2023Quote: The NucleoSpin® Tissue Kit (Macherey-Nagel, Düren, Germany) combined with the use of zirconia beads (BioSpec ...
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bioRxiv - Neuroscience 2023Quote: Total RNA was isolated from mouse tissues using the Nucleospin RNA Plus kit (Macherey-Nagel, cat# 740984.50), following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: Genomic DNA was extracted from only the 13C-acetate grown cultures that yielded enough DNA at 94 days of incubation using the NucleoSpin Microbial DNA kit (Macherey-Nagel) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... the cellular RNA was extracted by NucleoSpin RNA Kit (Macherey-Nagel GmbH&Co. KG, Germany) for real-time PCR analysis of SARS-CoV-2 E genes using the iTaq Universal Probes One-Step RT-PCR Kit (Bio-Rad ...
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bioRxiv - Microbiology 2023Quote: ... Low copy plasmids were extracted by NucleoBond Xtra Midi kit (MACHEREY-NAGEL) with the other plasmids were extracted by plasmids midi kit (QIAGEN ...
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bioRxiv - Plant Biology 2023Quote: PCR products were pooled by 6 to 12 according to the linkers and cleaned using the Nucleospin Gel and PCR clean up (Macherey-Nagel). Samples containing asymptomatic plants were pooled separately from those containing symptomatic leaves to limit potential cross-contaminations of highly concentrated viruses in symptomatic pools ...
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bioRxiv - Plant Biology 2023Quote: ... RNA was isolated was using the NucleoSpin® RNA Plant Kit (Macherey-Nagel, Düren, Germany) according to the manufacturer’s guidelines ...
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bioRxiv - Plant Biology 2023Quote: ... approximately 50 mg of ground tissue were extracted using the NucleoSpin RNA Plant Kit (Macherey-Nagel, Düren, Germany) following the user guidelines ...
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bioRxiv - Plant Biology 2023Quote: Total RNA was extracted from the roots of 21-days old mock- and SA190-colonized Arabidopsis grown on ½ MS with or without 25% PEG with the Nucleospin RNA plant kit (Macherey-Nagel) following the manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2023Quote: ... purified using the NucleoSpin plasmid kit (Macherey-Nagel, Düren, Germany), were confirmed by sequencing.
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bioRxiv - Microbiology 2023Quote: DNA was extracted for Termignon cheese isolates and all new strains sequenced using the Illumina technology for this project from fresh haploid mycelium and conidia after monospore isolation and growth for five days on malt agar using the Nucleospin Soil Kit (Macherey-Nagel). Sequencing was performed using the Illumina paired-end technology (Illumina Inc. ...
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bioRxiv - Microbiology 2023Quote: ... and NucleoBond® Xtra Midi Plus (MACHEREY-NAGEL, Düren, Germany) for small and large scales ...
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bioRxiv - Microbiology 2023Quote: ... Replicon RNA was extracted using NucleoSpin®RNA (MACHEREY-NAGEL) at the indicated time points and its copy number was measured using the THUNDERBIRD Probe One-step qRT-PCR Kit (TOYOBO CO. ...
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bioRxiv - Microbiology 2023Quote: Genomic DNA was extracted following standard protocols using the NucleoSpin Microbial DNA Mini kit (Macherey-Nagel, Düren, Germany). DNA was quantified using a Qubit 3 fluorometer in combination with dsDNA BR or HS assay kits (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... PCR products were purified using the Nucleospin® extract II kit (Macherey-Nagel) according to manufacturer’s instructions and were Sanger sequenced (Eurofins UK).
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bioRxiv - Microbiology 2023Quote: ... PCR-column purified (Macherey-Nagel), and sent for Sanger sequencing at Genewiz with the primers in Table 2.
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bioRxiv - Microbiology 2023Quote: ... 50 μl of a 40% slurry Ni-NTA agarose (20 μl beads, Macherey-Nagel) were added followed by an incubation for 45 min at 4°C with shaking ...
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bioRxiv - Molecular Biology 2023Quote: ... and rDNase (Macherey-Nagel) and reverse-transcribed with ReverTra Ace® (TOYOBO ...
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bioRxiv - Molecular Biology 2023Quote: ... The ligated products were transformed into XL1 blue bacterial cells by electroporation followed by isolating the plasmids from single bacterial colonies using NucleoBond Xtra Midi kit (Macherey-Nagel). The Cas9-sgRNA plasmids were validated by BbsI restriction analysis and confirmed by Sanger sequencing ...
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bioRxiv - Molecular Biology 2023Quote: ... kit with NTB binding buffer (#740595.150, Macherey-Nagel) according to the manufacturer’s instructions and eluted in 30 µL.
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bioRxiv - Pathology 2023Quote: ... we isolated mtDNA from postnuclear fractions with the alkaline denaturation-neutralization procedure (Nucleospin Plasmid Mini kit, Macherey-Nagel). Liver tissue was homogenized and nuclei pelleted (1000g 10 min ...
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bioRxiv - Plant Biology 2023Quote: ... Total DNA was extracted using CTAB (Doyle & Doyle 1987) and purified using NucleoSpin® Purification Kit (Macherey-Nagel, Düren, Germany). DNA extractions were profiled in Agarose gel 1% and using Nanodrop ...
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bioRxiv - Zoology 2023Quote: ... Germany) or on a hydrophilic interaction liquid chromatography (HILIC) Nucleodur column (125 × 3 mm, 3 μm, Macherey-Nagel, Düren, Germany) for normalphase chromatography ...
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bioRxiv - Plant Biology 2023Quote: Total RNA was extracted from Arabidopsis seedling tissues with NucleoZOL Reagent (Macherey-Nagel) according to the manufacturer’s protocol and gene expression analysis was carried out as previously described (Locci et al. ...