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Citations for Macherey-Nagel GmbH :
151 - 200 of 3263 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... and genomic DNA was extracted using the Nucleospin Blood L Midi genomic DNA extraction kit (Macherey-Nagel, 740954.20). Genomic DNA was PCR amplified by the Genetic Perturbation Platform at the Broad Institute using the following primers:
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bioRxiv - Cancer Biology 2024Quote: ... RNA was isolated using the NucleoSpin RNA Mini kit (Macherey-Nagel, #740955.250). DNase digestion was performed on a column as recommended by the manufacturer ...
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bioRxiv - Cancer Biology 2024Quote: RNA was isolated from cells with the NucleoSpin RNA Plus Kit (Macherey-Nagel, 740984) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... and clean-up using the NucleoSpin RNA Clean-up Mini Kit (Macherey-Nagel, #740948.50). RNA quality was surveyed using RNA ScreenTapes (Agilent ...
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bioRxiv - Developmental Biology 2024Quote: ... Plasmids were purified with the NucleoSpin Plasmid MiniprepKit (CAT 740588, Macherey-Nagel) and sequenced by Sanger sequencing ...
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bioRxiv - Microbiology 2024Quote: ... 15 and 30 days using the NucleoSpin™ Soil kit (Macherey-Nagel, Germany), and DNA was quantified with Quant-iT BR ds-DNA assay kit with a Qubit fluorometer (Invitrogen ...
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bioRxiv - Microbiology 2024Quote: ... followed by gel isolation with NucleoSpin Gel and PCR Clean-up (Macherey-Nagel) following the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2024Quote: ... RNA was column purified using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel) with NTC buffer according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2024Quote: ... by using the NucleoSpin RNA virus kit (Macherey-Nagel). For intracellular viral RNA cells were harvested in TRI Reagent and RNA extraction (Zymo Research ...
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bioRxiv - Cell Biology 2024Quote: ... and total RNA was extracted using NucleoZOL (NucleoZOL; Macherey-Nagel GmbH & Co., KG, Dylan, Germany). mRNA was reverse transcribed using an M5 Super qPCR RT kit with gDNA remover (MF012-T ...
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bioRxiv - Cell Biology 2024Quote: ... total RNA was isolated from hMBs using Nucleo-spin RNA Plus (Macherey-Nagel, Düren, Germany) and reverse transcribed using ReverTra Ace qPCR RT Master Mix (TOYOBO ...
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bioRxiv - Biophysics 2024Quote: ... NucleoSpin® plasmid isolation kit and NucleoSpin® gel and PCR clean-up kit were purchased from Macherey-Nagel (Germany). PierceTM protease inhibitor tablets EDTA free and Pierce™ high capacity Ni-IMAC resin were purchased from Thermo Scientific (international) ...
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bioRxiv - Molecular Biology 2024Quote: ... were extracted using the NucleoSpin Soil kit (Macherey-Nagel) with a final elution volume of 50 µl following the manufacturer instructions ...
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bioRxiv - Plant Biology 2024Quote: ... Lipid classes were separated using an Agilent 1200 HPLC system using a 150 mm × 3 mm (length × internal diameter) 5 µm diol column (Macherey-Nagel), at 40 °C ...
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bioRxiv - Microbiology 2024Quote: ... Cells were lysed mechanically via bead-beating and DNA was purified on columns (Macherey-Nagel, Germany). A robotized platform (Biomek4000 ...
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bioRxiv - Microbiology 2024Quote: ... the Genomic DNA from Microorganisms Kit (Macherey-Nagel, Germany) was used as per the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... The resulting amplicons were purified using the NucleoSpin gel and PCR cleanup kit following the manufacturer’s instructions (Macherey-Nagel Inc., Bethlehem, PA) and submitted for Sanger sequencing with the same primers used to generate the amplicons ...
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bioRxiv - Developmental Biology 2024Quote: ... and resistant clones then used for miniprep plasmid preparations (Nucleospin plasmid, Macherey-Nagel, Cat # 740588.50), followed by sequencing ...
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bioRxiv - Immunology 2024Quote: Total RNA was prepared using RNA purification kit (Macherey-Nagel GmbH & Co. KG, Germany), followed by cDNA synthesis (Bio-line ...
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bioRxiv - Immunology 2024Quote: ... DNA was purified with NucleoSpin™ gDNA Clean-up Kit (Macherey-Nagel GmbH & Co. KG, Germany). For ChIP-qPCR assays ...
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bioRxiv - Cancer Biology 2024Quote: ... The fixed cells were washed twice with PBS and stained with 20 µg/mL propidium iodide (PI) containing 100 mg/mL RNAse A (740505, MACHEREY-NAGEL) for 15 min at room temperature ...
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bioRxiv - Zoology 2024Quote: ... The purified plasmid was prepared using the NucleoBond® Xtra Midi kit (MACHEREY-NAGEL, Düren, Germany) or the Genopure Plasmid Midi kit (Roche Diagnostics GmbH ...
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bioRxiv - Cancer Biology 2024Quote: ... RNA was isolated from Eµ-Myc lymphoma cells using a NucleoSpin RNA kit (Macherey-Nagel) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... monocytes and hMDM using the NucleoSpin® RNA kit (Macherey-Nagel®) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... RNA extraction (Macherey-Nagel RNA extraction kit; Germany) was done according to supplier instructions ...
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bioRxiv - Biochemistry 2024Quote: ... Expressed proteins were captured on pre-packed Ni-IDA (Protino Ni-IDA Resin, Macherey-Nagel, Duren, Germany) columns ...
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bioRxiv - Developmental Biology 2024Quote: ... PCR products were run on gel electrophoresis before gel extraction of bands of interest (with sizes corresponding to either a wild-type allele or a deleted allele) (Nucleospin gel and PCR clean-up, Macherey-Nagel Cat# 740609.50). Fragments were cloned into blunt-end TOPO vector (Invitrogen Cat# 45024) ...
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bioRxiv - Microbiology 2024Quote: ... DNA was extracted using a Nucleospin PCR cleanup kit (Macherey-Nagel) and resuspended in 50 μl elution buffer (5 mM Tris-HCl [pH 8.5]) ...
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bioRxiv - Microbiology 2024Quote: ... Genomic DNA was then extracted following the Nucleospin Tissue Kit protocol (Macherey-Nagel) and resuspended in 50 μl elution buffer (5 mM Tris-HCl [pH 8.5]) ...
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bioRxiv - Microbiology 2024Quote: ... RNA was extracted using the RNA XS kit (Macherey-Nagel, Düren, Germany) from MS4 Ciona juveniles that underwent gut colonization ...
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bioRxiv - Cell Biology 2024Quote: ... and Ni-NTA Agarose resin (Macherey-Nagel) was mixed with the supernatant to capture PGL-3 protein molecules ...
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bioRxiv - Cell Biology 2024Quote: ... and Ni-NTA Agarose resin (Macherey-Nagel) was mixed with the supernatant to capture PGL-3 protein molecules ...
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bioRxiv - Cell Biology 2024Quote: ... and Ni-NTA Agarose resin (Macherey-Nagel) was mixed with the supernatant to capture PGL-3 protein molecules ...
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bioRxiv - Cancer Biology 2024Quote: ... coli using the Nucleobond Xtra Maxi EF Kit (Macherey-Nagel) and adequate representation of sgRNAs in the library was verified by next-generation sequencing and analysis with the python script count_spacers.py (Joung et al. ...
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bioRxiv - Cancer Biology 2024Quote: ... PCR amplicons were subjected to agarose gel size selection using the NucleoSpin Gel and PCR Clean-up Kit (Macherey-Nagel). Amplicons were cloned into BsmBIv2-digested (New England BioLabs ...
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bioRxiv - Cell Biology 2024Quote: ... Total RNA was isolated using the NucleoSpin RNA Mini kit (Macherey-Nagel) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... Plasmid DNA was isolated via the endotoxin-free NucleoBond Xtra Midi Plus EF kit (Macherey-Nagel). For transfection ...
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bioRxiv - Biochemistry 2024Quote: ... and purified using NucleoBond Xtra Midi kit (Macherey-Nagel GmbH, Duren, Germany). Directed site mutagenesis was made by substituting three amino acids in the ZapD sequence by Alanine (R20 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The isolation of gDNA was conducted either by using a column-based method following manufacturer’s instructions (NucleoSpin Microbial DNA kit, Macherey-Nagel, Düren, Germany) or an automated system (King Fisher Duo Prime System ...
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bioRxiv - Cell Biology 2024Quote: ... A large amount of these plasmid vectors was prepared using a NucleoBond Xtra Midi Plus kit (Macherey-Nagel, Düren, Germany). Concentrations of plasmid DNA were measured with a Nanodrop-1000 spectrophotometer (Thermo Fisher Scientific).
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bioRxiv - Developmental Biology 2024Quote: ... RNA was harvested at day 14 of the protocols and purified using the Nucleo Spin RNA (Macherey-Nagel) kit according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... the first-strand cDNA was purified with the NucleoSpin gel and PCR clean-up kit (Macherey-Nagel, no. 470609) following the manufacturer’s instruction ...
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bioRxiv - Cell Biology 2024Quote: Total RNA was extracted using a Nucleospin RNA Plus kit (Macherey-Nagel) and reverse transcribed using ReverTra Ace (TOYOBO Life Science ...
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bioRxiv - Immunology 2024Quote: ... followed by purification using NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel). Linearized DNA templates (1 µg ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The DNA pellet was digested in CF buffer (Macherey-Nagel) for 45 min at 65°C and purified using NucleoBond AXG20 Mini columns according to the user manual (Macherey-Nagel) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... for 45 min at 65°C and purified using NucleoBond AXG20 Mini columns according to the user manual (Macherey-Nagel). Final high molecular weight gDNA was quantified (Qubit) ...
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bioRxiv - Evolutionary Biology 2024Quote: We isolated total genomic DNA from silica-dried or herbarium leaf tissue using the NucleoSpin Plant II kit: Genomic DNA from plants (Macherey-Nagel, Düren, Germany) following a modified version of the manufacturer’s manual (Supplementary Data Table S4) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... we purified the amplification reaction following the PCR clean-up protocol of the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel, Düren, Germany). Finally ...
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bioRxiv - Developmental Biology 2024Quote: Total RNA was obtained from ∼50 zebrafish embryos using the NucleoSpin RNA extraction kit (Macherey-Nagel). cDNA libraries were then synthesized using the SMARTer PCR cDNA Synthesis Kit (Clontech ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Plasmids were isolated using the NucleoBond® PC 2000 Mega kit (MACHEREY-NAGEL). An aliquot of plasmid library was PCR-amplified with i5 and i7 primers and sequenced on Novaseq (150 bp Paired-end ...