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Citations for Macherey-Nagel GmbH :
151 - 200 of 3300 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: Total RNA was prepared using RNA purification kit (Macherey-Nagel GmbH & Co. KG, Germany), followed by cDNA synthesis (Bio-line ...
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bioRxiv - Immunology 2024Quote: ... DNA was purified with NucleoSpin™ gDNA Clean-up Kit (Macherey-Nagel GmbH & Co. KG, Germany). For ChIP-qPCR assays ...
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bioRxiv - Cancer Biology 2024Quote: ... The fixed cells were washed twice with PBS and stained with 20 µg/mL propidium iodide (PI) containing 100 mg/mL RNAse A (740505, MACHEREY-NAGEL) for 15 min at room temperature ...
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bioRxiv - Zoology 2024Quote: ... The purified plasmid was prepared using the NucleoBond® Xtra Midi kit (MACHEREY-NAGEL, Düren, Germany) or the Genopure Plasmid Midi kit (Roche Diagnostics GmbH ...
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bioRxiv - Cancer Biology 2024Quote: ... RNA was isolated from Eµ-Myc lymphoma cells using a NucleoSpin RNA kit (Macherey-Nagel) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... monocytes and hMDM using the NucleoSpin® RNA kit (Macherey-Nagel®) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... PCR products were run on gel electrophoresis before gel extraction of bands of interest (with sizes corresponding to either a wild-type allele or a deleted allele) (Nucleospin gel and PCR clean-up, Macherey-Nagel Cat# 740609.50). Fragments were cloned into blunt-end TOPO vector (Invitrogen Cat# 45024) ...
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bioRxiv - Microbiology 2024Quote: ... DNA was extracted using a Nucleospin PCR cleanup kit (Macherey-Nagel) and resuspended in 50 μl elution buffer (5 mM Tris-HCl [pH 8.5]) ...
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bioRxiv - Microbiology 2024Quote: ... Genomic DNA was then extracted following the Nucleospin Tissue Kit protocol (Macherey-Nagel) and resuspended in 50 μl elution buffer (5 mM Tris-HCl [pH 8.5]) ...
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bioRxiv - Cell Biology 2024Quote: ... and Ni-NTA Agarose resin (Macherey-Nagel) was mixed with the supernatant to capture PGL-3 protein molecules ...
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bioRxiv - Cell Biology 2024Quote: ... and Ni-NTA Agarose resin (Macherey-Nagel) was mixed with the supernatant to capture PGL-3 protein molecules ...
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bioRxiv - Cell Biology 2024Quote: ... and Ni-NTA Agarose resin (Macherey-Nagel) was mixed with the supernatant to capture PGL-3 protein molecules ...
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bioRxiv - Cancer Biology 2024Quote: ... coli using the Nucleobond Xtra Maxi EF Kit (Macherey-Nagel) and adequate representation of sgRNAs in the library was verified by next-generation sequencing and analysis with the python script count_spacers.py (Joung et al. ...
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bioRxiv - Cancer Biology 2024Quote: ... PCR amplicons were subjected to agarose gel size selection using the NucleoSpin Gel and PCR Clean-up Kit (Macherey-Nagel). Amplicons were cloned into BsmBIv2-digested (New England BioLabs ...
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bioRxiv - Cell Biology 2024Quote: ... Total RNA was isolated using the NucleoSpin RNA Mini kit (Macherey-Nagel) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... Plasmid DNA was isolated via the endotoxin-free NucleoBond Xtra Midi Plus EF kit (Macherey-Nagel). For transfection ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The isolation of gDNA was conducted either by using a column-based method following manufacturer’s instructions (NucleoSpin Microbial DNA kit, Macherey-Nagel, Düren, Germany) or an automated system (King Fisher Duo Prime System ...
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bioRxiv - Cell Biology 2024Quote: ... A large amount of these plasmid vectors was prepared using a NucleoBond Xtra Midi Plus kit (Macherey-Nagel, Düren, Germany). Concentrations of plasmid DNA were measured with a Nanodrop-1000 spectrophotometer (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2024Quote: ... the first-strand cDNA was purified with the NucleoSpin gel and PCR clean-up kit (Macherey-Nagel, no. 470609) following the manufacturer’s instruction ...
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bioRxiv - Cell Biology 2024Quote: Total RNA was extracted using a Nucleospin RNA Plus kit (Macherey-Nagel) and reverse transcribed using ReverTra Ace (TOYOBO Life Science ...
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bioRxiv - Immunology 2024Quote: ... followed by purification using NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel). Linearized DNA templates (1 µg ...
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bioRxiv - Developmental Biology 2024Quote: Total RNA was obtained from ∼50 zebrafish embryos using the NucleoSpin RNA extraction kit (Macherey-Nagel). cDNA libraries were then synthesized using the SMARTer PCR cDNA Synthesis Kit (Clontech ...
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bioRxiv - Developmental Biology 2024Quote: ... PCR product was then purified by following NucleoSpin PCR Clean-up procedure (Macherey-Nagel; Cat# 740609.250) and sent for Sanger sequencing to confirm genotypes.
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bioRxiv - Microbiology 2024Quote: ... DNA fragments and PCR products were purified using the Nucleospin Gel and PCR clean up kit (Macherey-Nagel).
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bioRxiv - Microbiology 2024Quote: ... The PCR amplicons were gel purified with NucleoSpin gel and PCR clean-up kits (Macherey-Nagel, Germany) following the manufacturer’s protocol ...
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bioRxiv - Genetics 2024Quote: ... Maxi kit (Macherey-Nagel). Cell culture was performed whenever the cells had grownto 90% of the cell plate.
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bioRxiv - Genetics 2024Quote: ... the plasmid library was obtained using NucleoBond Xtra Midi EF kit (Macherey-Nagel). The oligo library was confirmed using nested PCR and Illumina sequencing.
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bioRxiv - Evolutionary Biology 2024Quote: ... Peptides were desalted by using C18 solid phase extraction cartridges (Macherey-Nagel). Cartridges were prepared by adding acetonitrile (ACN) ...
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bioRxiv - Genomics 2024Quote: ... dissolved in TE buffer (10 mM Tris pH8, 1 mM EDTA pH8) and purified using NucleoSpin Gel and PCR clean-up (Macherey-Nagel). DNA was loaded on a 2% agarose gel ...
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bioRxiv - Genomics 2024Quote: ... Cross-linking was reversed by overnight incubation at 65°C and DNA fragments were purified using NucleoSpin Gel and PCR Clean-up columns (Macherey-Nagel).
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bioRxiv - Evolutionary Biology 2024Quote: ... genomic DNA extractions were performed using the NucleoSpin Microbial DNA Mini kit (Macherey-Nagel) following manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and the NucleoBond HMW DNA kit (Macherey-Nagel, North Rhine-Westphalia, Germany) eluted in 100 µl–200 µl low-pH TE buffer ...
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bioRxiv - Genetics 2024Quote: ... DNAs were extracted using NucleoSpin Blood XL (Macherey-Nagel#740950). Samples were prepared for sequencing as previously described35.
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bioRxiv - Genomics 2024Quote: DNA was extracted using either the OmniPrep Genomic DNA Purification Kit (G Biosciences, St. Louis, MO, USA) or the Nucleospin Plant II midi DNA Extraction Kit (Macherey-Nagel, Düren, Germany). DNA quality was assessed using a Qubit fluorometer (Themo Fisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... Total RNA was extracted from frozen powder with the NucleoSpin® RNA kit (Macherey-Nagel, 740955). RNA samples were concentrated with NucleoSpin® RNA Clean-up XS (Macherey-Nagel ...
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bioRxiv - Genetics 2024Quote: ... while the ZB5d/antibiotics mixture was further processed with the NucleoMag VET Kit (Macherey-Nagel, Düren, Germany) in a final elution volume of 100µL VEL-buffer using a KingFisher®Flex automat (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2024Quote: ... Resulting PCR products were purified with the Bioanalysis NucleoSEQ Kit (Macherey-Nagel), and 15 µL of each eluate was mixed with the same volume of Hi-Di formamide (Thermo Fisher Scientific) ...
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bioRxiv - Genetics 2024Quote: Total RNA from twenty heads of 3 day-old Drosophila adults for each genetic combination was purified using the RNA isolation kit of Macherey-Nagel (Macherey-Nagel, Düren, Germany) according to the manufacturer’s instructions.1 μg of total RNA was reverse transcribed using the High-Capacity cDNA Archive Kit (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2024Quote: ... RNA & Protein Purification kit (Macherey-Nagel). Clones were screened by XhoI digestion and Sanger sequencing.
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bioRxiv - Immunology 2024Quote: ... RNA samples were concentrated with NucleoSpin® RNA Clean-up XS (Macherey-Nagel, 740903) or GeneJet RNA Cleanup and Concentration Micro Kit (Life Technologies ...
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bioRxiv - Genomics 2024Quote: ... each side of the Amicon filter was rinsed and incubated at 56°C for 1 hour (per side) in 200 µL Buffer T1 and 20 µL Proteinase K from the NucleoSpinl1J Tissue Kit (MACHEREY-NAGEL Inc., Allentown, PA, United States) and DNA extraction proceeded from step 3 of the kit ...
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bioRxiv - Microbiology 2024Quote: PCR products were purified using NucleoSpin Gel and PCR Clean-up (Macherey-Nagel) according to manufacturer’s recommendations ...
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bioRxiv - Microbiology 2024Quote: ... PCR clean-up (Macherey-Nagel Cat. No. 740609.250S), and Sanger sequencing (Microsynth GmbH ...
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bioRxiv - Microbiology 2024Quote: ... PCRs were purified using NucleoSpin® Gel and PCR Clean-up kit (Macherey-Nagel). Plasmids and PCRs were digested with the appropriate restriction enzymes (New England Biolabs) ...
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bioRxiv - Microbiology 2024Quote: ... RNA was column purified using NTC buffer and the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel), according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2024Quote: Large and small (including miRNAs) RNA fractions were isolated from the same macrophage samples using the NucleoSpin miRNA kit (Macherey-Nagel) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... Total DNA and RNA was extracted using the NucleoMag VET extraction kit (Macherey-Nagel, Germany) as described by the manufacturer’s instructions with the IDEALTM 96 extraction robot (Innovative Diagnostics ...
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bioRxiv - Microbiology 2024Quote: ... DNA was extracted using 100 µL of homogenate with the Genomic DNA tissue kit (Macherey-Nagel, Germany). Detection and quantification of R ...
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bioRxiv - Immunology 2024Quote: Serum samples from all experimental groups were extracted using the NucleoSpin RNA isolation kit (Macherey-Nagel, Allentown, PA). Following viral RNA extraction ...
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bioRxiv - Microbiology 2024Quote: ... The PCR products were purified using NucleoSpin Gel and PCR Cleanup Kit (Macherey-Nagel), following the manufacturer’s instructions ...