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Citations for Macherey-Nagel GmbH :
401 - 450 of 3300 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... Amplified regions of CRISPR-C unedited samples and the specific bands derived from the amplification of the deletion scar and circular junction sites were dissected and DNA products purified by NucleoSpin Gel and PCR Clean-up kit (MACHEREY-NAGEL Düren, Nordrhein-Westfalen, Germany) and sent for Sanger sequencing at Eurofins Genomics ...
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bioRxiv - Molecular Biology 2024Quote: ... Total RNA was extracted from the primary CEC lines using a NucleoSpin RNA XS kit (Macherey-Nagel), in accordance with the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... Twenty percent of the reaction mixture was spotted onto a polyethyleneimine cellulose plate (MACHEREY-NAGEL) that had been pre-run with water for 2 h ...
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bioRxiv - Plant Biology 2024Quote: ... RNA was then isolated with the NucleoSpin RNA Plant and Fungi kit (Macherey-Nagel, Cat# 740120.5) with on-column DNA digestion ...
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bioRxiv - Microbiology 2024Quote: Total RNAs were purified using NucleoSpin RNA (Macherey-Nagel) and reverse-transcribed into cDNAs using ReverTra Ace (Toyobo ...
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bioRxiv - Microbiology 2024Quote: RNA of WD-NECs was extracted using the Nucleospin RNA Plus Kit (Macherey-Nagel) according to manufacturer’s protocol ...
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bioRxiv - Plant Biology 2024Quote: Total RNAs were extracted from 100 mg of tissue with the Nucleospin RNA plant (Macherey-Nagel) kit following the manufacturer instructions ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: RNA purification and reverse transcription were performed using the Nucleospin RNA kit from Macherey-Nagel (Düren, Germany) and the High capacity cDNA reverse transcription kit from Applied Biosystems (Woolston ...
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bioRxiv - Plant Biology 2024Quote: ... total RNA was extracted from plants grown on plates using NucleoSpin RNA extraction kit (Macherey-Nagel GmbH & Co.). First-strand cDNA was synthesized using 2 μg RNA and PrimeScript RT Master Mix (Takara Bio) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... DNA extractions were either performed with a modified CTAB 2%/PVPP 2% protocol (see Jolly et al. 2003) or the NucleoSpin®Tissue 96 kit (Macherey-Nagel, Karlsruhe, Germany).
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bioRxiv - Cell Biology 2024Quote: ... Plasmids were purified using a maxiprep kit (Macherey-Nagel, #740574.25) and sequenced ...
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bioRxiv - Microbiology 2024Quote: ... The last purification step was performed with the RNA Nucleospin kit according to manufacturer’s instructions (Macherey-Nagel). The integrity of the RNAs was verified using the Agilent 2100 bioanalyzer with the RNA 6000 Nano kit (Agilent Technologies) ...
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bioRxiv - Plant Biology 2024Quote: ... For recombinant His-UBA1 and His-UBC8 protein were purified using Ni-Ted resin (Macherey-Nagel).
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bioRxiv - Cancer Biology 2024Quote: RNA was isolated from cells using NucleoSpin RNA Plus (Macherey-Nagel, Duren, Germany, Cat. No. 740984.250) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... and DNA was extracted using Nucleospin Gel and PCR Clean-up kit (Macherey-Nagel Cat. 740609) and cleaned with QIAGEN MinElute PCR purification kit (QIAGEN cat ...
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bioRxiv - Evolutionary Biology 2024Quote: ... crushed the samples using five MN Beads Type D (Macherey-Nagel). We crushed leg samples with only three beads ...
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bioRxiv - Evolutionary Biology 2024Quote: ... We extracted total genomic DNA from each whole individual sample using NucleoMag® Tissue Kit (Macherey-Nagel) according to the manufacturer’s recommendations ...
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bioRxiv - Genomics 2024Quote: ... from which HMW DNA was isolated by NucleoBond HMW DNA kit (Macherey-Nagel), following the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2024Quote: ... or NucleoSpin® Tissue Kit (Macherey-Nagel, Düren, Germany). Otoliths were fully submerged in the lysis buffer to lysate any remnant tissues then removed from the buffer ...
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bioRxiv - Cell Biology 2024Quote: ... RNA was extracted using a modified protocol of the Nucleospin 96 RNA kit (Macherey-Nagel, Duren, Germany). Cells were lysed in a 96 deep-well plate by adding 450μl of lysis buffer containing 1M sorbitol (Sigma-Aldrich) ...
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Assessment of the transcriptional regulation of EMT and MET through accessible transposable elementsbioRxiv - Genomics 2024Quote: Isolation of RNA samples was performed with the Nucleospin RNA II kit (Macherey-Nagel) as recommended by the manufacturer ...
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bioRxiv - Microbiology 2024Quote: RNA was extracted from parasites using NuceloSpin RNA kit (Macherey-Nagel). cDNA was obtained using SuperScript III First Strand Synthesis SuperMix for RT-qPCR (Invitrogen ...
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bioRxiv - Physiology 2024Quote: ... RNA was extracted from the homogenised pallium samples using the NucleoSpin RNA Plus kit (MACHEREY-NAGEL). Since the sample size of the control group was the main factor limiting statistical power ...
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bioRxiv - Cancer Biology 2024Quote: ... The RNA of the sorted cells was extracted using a Nucleospin RNA XS kit (Macherey-Nagel), and its concentration was measured using QuBit ...
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bioRxiv - Cancer Biology 2024Quote: Total RNAs were extracted using nucleospin RNA II (Macherey-Nagel), reverse-transcribed using superscript IV (Invitrogen) ...
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bioRxiv - Biochemistry 2024Quote: ... and 51μm filters using Macherey-Nagel a NucleoMag RNA kit (Macherey-Nagel GmbH & Co.KG). Cleared lysate was loaded into a 96 deep-well plate and put on an epMotion 5075 TMX liquid handler to complete the RNA extraction following the Machery-Nagel standard protocol ...
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bioRxiv - Biochemistry 2024Quote: RNA from 10 mL yeast culture was isolated using a mini kit for RNA isolation (Macherey-NAGEL, REF 740933.50). Next ...
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bioRxiv - Biochemistry 2024Quote: ... after which positive colonies were purified using a Nucleospin Plasmid miniprep kit (Macherey-Nagel) and sent for Sanger Sequencing on an Applied Biosystems 3730XL DNA Analyzer (Neuromics Support Facility ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... RNA extraction was carried out with the NucleoSpin RNA kit (Macherey-Nagel, #740955) following the guidelines provided by the manufacturer ...
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bioRxiv - Cell Biology 2024Quote: ... Nucleospin Gel and PCR clean-up kit (Macherey-Nagel) was employed to purify DNA from the gel ...
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bioRxiv - Microbiology 2024Quote: ... was performed either when all ammonium (or nitrate) and nitrite was depleted, or when the values stayed constant for 1-3 days (qualitatively, using Quantofix (Macherey-Nagel) strips) ...
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bioRxiv - Microbiology 2024Quote: One hundred μl of the bacteria cultured in TSB as described above were pipetted onto a filter paper (Ø 25 mm, Macherey-Nagel, Germany) and placed in the glass odour adapters with the help of sterile tweezers ...
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bioRxiv - Neuroscience 2024Quote: ... and Nucleospin RNAII Kit (Macherey-Nagel) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2024Quote: ... RNA was extracted using the NucleoSpin RNA Plus kit (Macherey-Nagel) and cDNA was synthesized with the RevertAid First Strand cDNA Synthesis Kit (Thermo Scientific) ...
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bioRxiv - Plant Biology 2024Quote: ... and the resulting PCR reactions were cleaned-up using the NucleoSpin Gel and PCR Clean-up XS kit (Macherey-Nagel). All amplicons obtained from a single biological replicate were pooled in equimolar amounts and a total of 100 ng were used for library preparation following the modified Illumina Nextera Flex library preparation protocol described before (28 ...
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bioRxiv - Genomics 2024Quote: ... the DDM-free peptide samples were desalted with 0.5 % formic acid and eluted with 0.5 % formic acid/80 % acetonitrile using SepPak C18 cartridges (Waters, Milford, MA, USA) with the help of CHROMABOND® SPE vacuum manifold (Macherey-Nagel GmbH & Co. KG, Duren, Germany). Samples were then dried in a Savant SpeedVac SPD120 (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2024Quote: ... The soluble fraction was applied on TBS-equilibrated Protino Ni-IDA resin (Macherey-Nagel, Germany), washed ...
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bioRxiv - Biochemistry 2024Quote: ... followed by nickel affinity purification (Ni-IDA, Macherey-Nagel) after lysis ...
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bioRxiv - Cancer Biology 2024Quote: ... and isolated using NucleoBond Xtra Midi (Macherey-Nagel, Germany) according to the protocol from the manufacturer ...
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bioRxiv - Evolutionary Biology 2024Quote: ... DNA was extracted from 570 foot tissue samples using NucleoSpin® Tissue kits (Macherey-Nagel) or a CTAB protocol (Doyle & Dickson ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 80 µL of N-methyl-N-trimethylsilyl-trifluoroacetamid (Macherey-Nagel) were added ...
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bioRxiv - Synthetic Biology 2024Quote: ... DNA fragments were purified with NucleoSpin™ gel and PCR clean-up kits (Macherey-Nagel, Germany). Synthetic promoters were ordered from IDT (Integrated DNA Technologies) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... we used the NucleoSpin Blood QuickPure Kit (Macherey-Nagel). Extract quality was first assessed on a 1.5% agarose gel to exclude extractions with insufficient yield or excessively degraded DNA ...
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bioRxiv - Cell Biology 2024Quote: ... and total RNA was collected using a NucleoSpin RNA kit (Macherey-Nagel, #740955). Two technical replicates for each condition were pooled together to obtain an experimental replicate ...
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bioRxiv - Microbiology 2024Quote: ... PCR-amplified DNA was cleaned with NucleoSpin Gel and PCR Clean-up kit (MACHEREY-NAGEL, Düren, Germany). Plasmid DNA was purified using QIAprep Spin Miniprep Kit (QIAGEN ...
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bioRxiv - Microbiology 2024Quote: ... the targeted one was purified from a gel using the NucleoSpin® Gel and PCR Clean-up (MACHEREY-NAGEL, Düren, Germany) or the Monarch® DNA Gel Extraction Kit (New England Biolabs) ...
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bioRxiv - Molecular Biology 2024Quote: ... DNA was extracted from the filters using Nucleospin eDNA water kit (Macherey-Nagel) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... Genomic DNA was isolated using Nucleospin Tissue kit (Macherey-Nagel, Germany) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... The cultures were pelleted and resuspended in Nucleoprotect RNA (Macherey-Nagel) according to manufacturer’s protocol (41) ...
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bioRxiv - Molecular Biology 2024Quote: ... or the NucleoBond Xtra MIDI EF kit (Macherey-Nagel). For all the plasmids that were generated ...