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Citations for Macherey-Nagel GmbH :
401 - 450 of 3263 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... Modified BAC DNA was prepared using the large construct DNA purification kit - NucleoBond® Xtra BAC (Macherey-Nagel), linearized with PmeI and purified through a Sepharose™ separation column68 ...
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bioRxiv - Neuroscience 2024Quote: ... BAC DNA for pronuclear injection was prepared using the large construct DNA purification kit - NucleoBond® Xtra BAC (Macherey-Nagel), linearized with PI-SceI and purified over a home-made Sepharose CL4B column (Johansson et al. ...
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bioRxiv - Molecular Biology 2024Quote: ... PCR products were separated on 2.5% agarose gels and extracted using a NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel, Düren, Germany, cat. no. 740609). The purified PCR products were then cloned using a Zero Blunt TOPO PCR Cloning Kit (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... following the manufacturer’s instruction (Macherey-Nagel, Bethlehem, PA). 5′ RACE was carried out using SMARTer RACE 5′/3′ Kit (Takara Bio USA ...
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bioRxiv - Biochemistry 2024Quote: Total RNA was extracted from the cultured HEK293T cells using NucleoSpin RNA Plus (MACHEREY-NAGEL, #740984). mRNA was isolated using Dynabeads Oligo(dT)25 (Invitrogen ...
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bioRxiv - Biochemistry 2024Quote: ... Preparative HPLC of peptides was done on a Gilson PLC 2020 system using a Nucleodur C18 Htec Spum column (Macherey-Nagel, 100 A, 5 m, 250 mm x 32 mm, 30 mL/min). The following gradient was used in all purifications ...
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Spirolactone, an unprecedented antifungal β-lactone spiroketal macrolide from Streptomyces iranensisbioRxiv - Biochemistry 2024Quote: ... Plasmid DNA extraction was performed using NucleoSpin Plasmid EasyPure Kit (Macherey-Nagel, Germany). DNA purification was conducted on 1% Tris-acetate-EDTA (TAE ...
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Spirolactone, an unprecedented antifungal β-lactone spiroketal macrolide from Streptomyces iranensisbioRxiv - Biochemistry 2024Quote: ... DNA purification was conducted on 1% Tris-acetate-EDTA (TAE) agarose gel followed by using NucleoSpin Gel and PCR Clean-up Kits (Macherey-Nagel, Germany). One Shot™ Mach1™ T1 Phage-Resistant Chemically Competent E ...
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bioRxiv - Genetics 2024Quote: ... bands were cut from the gel and extracted using column purification (Macherey-Nagel, REF-740609.250). Samples were submitted for sequencing at Azenta/Genewiz to confirm gene deletions or fragment insertions.
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bioRxiv - Genomics 2024Quote: The RNA from 3 independent replicates (100mg/replicate) per regime were extracted using the Macherey-Nagel Mini kit for RNA purification (Macherey-Nagel, Allentown, PA). Sample purity was assessed using the NanoDrop ND-1000 Spectrophotometer (NanoDrop ...
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bioRxiv - Genomics 2024Quote: ... genomic DNA was extracted from whole adult flies using the NucleoMag Tissue Kit (Macherey-Nagel), and a total of 50 ng was used as a template for PCR amplifications encompassing ebony exons e1 and e2 with OneTaq HS Quick-Load Master Mix with Standard Buffer (NEB ...
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bioRxiv - Evolutionary Biology 2024Quote: ... nerve chain and ovaries of each female was extracted using standard protocols (DNA used for ONT sequencing: Macherey-Nagel NucleoBond HMW DNA kit ...
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bioRxiv - Developmental Biology 2024Quote: ... and purified using a NucleoSpin RNA Clean-up kit (MACHEREY-NAGEL) for RNA-seq analysis.
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bioRxiv - Genomics 2024Quote: ... total DNA was extracted from whole adult flies using the NucleoMag Tissue Kit (Macherey-Nagel) and used as a template for a two-step PCR with Phusion High-Fidelity DNA polymerase (NEB ...
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bioRxiv - Genomics 2024Quote: DNA was extracted from 1×106 cells using a standard protocol (Macherey-Nagel Nucleospin). Sequencing library preparation was performed using the KAPA HyperPlus Library Preparation kit and associated protocol ...
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bioRxiv - Physiology 2024Quote: ... and NMT-treated nonmyr-peptide were spotted (2 µL/spot) on TLC sheets (SIL/UV254, Macherey-Nagel) and separated for about 30 min in a mixture of n-butanol ...
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bioRxiv - Physiology 2024Quote: Total RNA was isolated using NucleoSpin RNA Plus kit (Macherey-Nagel) following the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2024Quote: ... The sgRNA library plasmid DNA was extracted by using NucleoBond Xtra Midi kit (Macherey-Nagel).
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bioRxiv - Synthetic Biology 2024Quote: ... All amplified DNA fragments were purified using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel), according to the vendor’s instructions ...
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bioRxiv - Plant Biology 2024Quote: RNA extraction utilized the Nucleospin RNA kit from Macherey-Nagel. Subsequently ...
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bioRxiv - Synthetic Biology 2024Quote: ... the next day plasmid DNA was extracted (Nucleospin Plasmid, Macherey-Nagel) and sent for Sanger sequencing (Eurofins Genomics) ...
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bioRxiv - Plant Biology 2024Quote: ... DNA was extracted from 3 to 4 leaves of 45 days old plants with a genomic DNA extraction kit (Macherey-Nagel, England). The DNAs of these leaves were combined to sequence the methylome of each plant ...
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bioRxiv - Synthetic Biology 2024Quote: ... extracted lipids were separated using silica gel-coated thin-layer chromatography (TLC) (0.25 mm Silica gel, DCFertigplatten, Macherey-Nagel, Germany) in a solvent mixture consisting of hexane ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... RNA was cleaned up using the NucleoSpin RNA kit (Macherey-Nagel), following the standard protocol ...
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bioRxiv - Plant Biology 2024Quote: ... Twenty seedlings were pooled per replicate and total RNA was extracted from homogenised plant tissue using a phenol-based RNA isolation method (NucleoZol, Macherey-Nagel, Catalogue No.: 740404). PolyA+ libraries were prepared by and sequenced at BGI Genomics (Hong Kong ...
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bioRxiv - Physiology 2024Quote: RNA isolation was performed using the NucleoSpin®RNA Kit (Macherey-Nagel, Germany) according to the manufacturers’ instructions ...
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bioRxiv - Plant Biology 2024Quote: ... The separation of substrates was performed at 35°C with a Nucleoshell RP18 column (50 × 3mm, particle size 2.7 μm; Macherey-Nagel, Germany) using an Agilent 1290 High-Pressure Liquid Chromatography (HPLC ...
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bioRxiv - Plant Biology 2024Quote: ... Genomic DNA was extracted using the NucleoSpin Plant II kit (Macherey-Nagel), and converted with the EZ DNA Methylation Kit (Zymo Research) ...
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bioRxiv - Cell Biology 2024Quote: ... RNA was isolated utilizing an RNA and protein purification kit (Macherey-Nagel, Düren, Germany) as per the manufacturer’s guidelines ...
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bioRxiv - Evolutionary Biology 2024Quote: We used the Nucleospin Soil Kit (Macherey-Nagel, Düren, Germany) for DNA extraction of the 68 newly isolated strains grown for five days on malt extract agar ...
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bioRxiv - Cell Biology 2024Quote: ... about 1×103 – 1×104 cells were subjected to total RNA isolation using NucleoSpin RNA Plus XS (MACHEREY-NAGEL GmbH & Co. KG). The first-strand cDNA was synthesized by SuperScript III (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... Genomic DNA was then isolated using NucleoSpin DNA RapidLyse kit (Macherey-Nagel). For fluorescence-sorted cells ...
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bioRxiv - Cell Biology 2024Quote: ... The genomic DNA from these sorted cells was then extracted using the NucleoSpin Tissue XS kit (Macherey-Nagel).
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bioRxiv - Cell Biology 2024Quote: ... Plasmids from single clones were isolated via miniprep (Macherey-Nagel 740499) and sequenced to confirm successful cloning.
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bioRxiv - Cell Biology 2024Quote: ... and the desired fragment was gel-purified (Macherey-Nagel 740609). Components of the insert were PCR amplified using Q5-Hot Start DNA Polymerase (NEB M0493 ...
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bioRxiv - Cell Biology 2024Quote: ... The dsDNA donors were purified using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel), and then either stored at -20°C or used directly for electroporation ...
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bioRxiv - Cell Biology 2024Quote: ... Enzyme-resistant dbDNA was then column-purified using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel). When 18 mol of plasmid template is reacted and subsequently eluted in 30 µL of sterile MQ ...
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bioRxiv - Genetics 2024Quote: ... and purified using NucleoSpin Gel and PCR Clean-up Kit (Macherey-Nagel). Alleles generated by genome editing are schematized in Fig ...
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bioRxiv - Evolutionary Biology 2024Quote: ... kits for NE English and Danish samples and NucleoSpin® 96 Tissue kits (Macherey-Nagel) for French samples ...
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bioRxiv - Plant Biology 2024Quote: ... 200 mM KCl) prior to purification on a Nucleobond AX-100 column (Macherey-Nagel). The column-bound RNA was washed twice with wash buffer WB (10 mM Tris-HCl ...
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bioRxiv - Microbiology 2024Quote: ... The DNA was extracted from the pellet using the instructions provided by a commercial kit (Macherey-Nagel GmbH & Co.). Each reaction consisted of 500 nM of primer FWD and REV (Table S3 ...
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bioRxiv - Microbiology 2024Quote: ... DNA extraction kits (Macherey-Nagel GmbH & Co.), protein ladder and 1 Kb DNA ladder (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2024Quote: ... MDDCs were lysed and total RNA was extracted using the NucleoSpin RNA Mini kit for RNA purification (Macherey-Nagel, 740955) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2024Quote: The RNA was extracted from fibroblast by NucleoSpin® RNA extraction kit (Macherey-Nagel #740955) and reverse transcribed by Maxima first strand cDNA synthesis kit (Thermo Fischer ...
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bioRxiv - Microbiology 2024Quote: ... The precipitated DNA was purified with a NucleoSpin gDNA Clean-up Kit (Macherey-Nagel, Düren, Germany) and then dissolved in a Tris-ethylenediaminetetraacetic acid (Tris-EDTA ...
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bioRxiv - Genomics 2024Quote: ... tissues were immediately frozen in liquid nitrogen and stored at -80°C prior to total RNA isolation with NucleoSpin RNA Plant and Fungi kit (Macherey-Nagel Inc., PA, US). RNA libraries for RNA-Seq were prepared using the Illumina TruSeq Stranded Total RNA Ribo-Zero Plant kit (Illumina ...
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Assessment of the transcriptional regulation of EMT and MET through accessible transposable elementsbioRxiv - Genomics 2024Quote: RNA was prepared using the Nucleospin RNA II kit (Macherey-Nagel) as recommended by the manufacturer ...
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bioRxiv - Genetics 2024Quote: ... using Ion Xpress™ Plus Fragment Library Kit (Thermo Scientifc, USA) and NucleoMag® NGS Clean-up and Size Select beads (Macherey-Nagel, Germany) following manufacturers’ instructions ...
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bioRxiv - Genetics 2024Quote: ... and purified using NucleoSpin Gel and PCR Clean-up Kit (Macherey-Nagel).
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bioRxiv - Genetics 2024Quote: ... and the resulting RNA pellet was further purified and DNAse treated using the Nucleospin RNA Clean-up Kit (Macherey-Nagel, Düren, Germany) according to the manufacturer’s protocol ...