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Citations for Macherey-Nagel GmbH :
551 - 600 of 3300 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2024Quote: ... Total RNA was extracted from the plants using the NucleoSpin RNA Plant Kit (Macherey-Nagel). Seven biological replicates were prepared for each condition ...
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bioRxiv - Plant Biology 2024Quote: ... Lipid classes were separated using an Agilent 1260 Infinity II HPLC system using a 150 mm×3 mm (length × internal diameter) 5 µm diol column (Macherey-Nagel), at 40°C ...
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bioRxiv - Immunology 2024Quote: Total RNA was extracted using the total RNA and protein isolation kit (Macherey-Nagel). RNA samples were reverse-transcribed with High-Capacity cDNA Reverse Transcription reagents kit (Applied Biosystems ...
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bioRxiv - Microbiology 2024Quote: ... The supernatant was cleared by centrifugation at 18,000 rpm and subsequently loaded onto Ni-IDA resin (Macherey-Nagel). The resin was washed with lysis buffer and the sample was eluted with lysis buffer supplemented with 100 mM imidazole ...
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bioRxiv - Molecular Biology 2024Quote: ... France) was added before purification using the NucleoSpin RNA Clean Up kit (740948-50; Macherey-Nagel, Düren, Germany). Prior to injection ...
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bioRxiv - Molecular Biology 2024Quote: ... and purified using the NucleoSpin plasmid Mini kit (Macherey-Nagel) or the NucleoBond Xtra MIDI EF kit (Macherey-Nagel) ...
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bioRxiv - Molecular Biology 2024Quote: ... PCR products were separated by agarose gel electrophoresis and purified from agarose gels using the NucleoSpin kit (Macherey-Nagel). Purified PCR products were subcloned into linearized plasmid vectors of interest using the In-fusion cloning system following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... Plasmid DNA was isolated using NucleoSpin Plasmid Mini Kit (Macherey-Nagel). The accuracy of the assemblies was verified by Sanger sequencing at Eurofins Genomics.
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bioRxiv - Molecular Biology 2024Quote: ... DNA purification was performed with NucleoMag kit for clean-up and size selection using NucleoMag SEP Mini separation plate (Macherey-Nagel). 4 ng of isolated DNA were used to complete the libraries by attaching indexes with xGen NXT UDI Primers (IDT) ...
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bioRxiv - Neuroscience 2024Quote: The NucleoSpin RNA extraction kit (Macherey-Nagel) was used to purify the RNA from the cells ...
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bioRxiv - Microbiology 2024Quote: ... RT-PCR products were purified with NucleoSpin® Gel and PCR Clean-up (Macherey-Nagel, Carlo Erba, Italy). Sequencing libraries were made with a Nextera-XT DNA Library Prep Kit (Illumina Inc. ...
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bioRxiv - Physiology 2024Quote: ... the lysate-ethanol mixture was applied to RNA columns (Macherey-Nagel) and the RNA was further processed according to the manufacturer’s instructions ...
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bioRxiv - Physiology 2024Quote: ... we used the NucleoSpin® RNA kit (Macherey-Nagel). Cells were lysed by adding RA1 supplemented with 1 % β-mercaptoethanol (Sigma) ...
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bioRxiv - Plant Biology 2024Quote: ... Raw DNA extracts were further purified using a NucleoSpin Soil kit (Macherey-Nagel) following the manufacturer’s protocol from step 4 (addition of SL3 buffer) ...
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bioRxiv - Plant Biology 2024Quote: ... Positive colonies were inoculated into 5 ml of LB medium with kanamycin (50 μg.mL−1) for overnight cultures at 30°C in order to isolate cloned plasmids using the Nucleospin plasmid kit (Macherey-Nagel). Identities of the cloned sequences were confirmed by Sanger sequencing using pHREAC sequencing primers (Table S6) ...
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bioRxiv - Plant Biology 2024Quote: Genomic DNA was extracted from flash-frozen young leaves using NucleoSpin Plant II kit (Macherey-Nagel) as per manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... Genomic DNA was extracted from the bacterial strains using the NucleoSpin@ Soil kit (Macherey-Nagel, Germany). A portion of the 16S rRNA genes was amplified using the primer pair (515F-TCG TCG GCA GCG TCA GAT GTG TAT AAG AGA CAG GTG CCA GCM GCC GCG GTAA and 806R-GTC TCG TGG GCT CGG AGA TGT GTA TAA GAG ACA GGG ACT ACH VGG GTW TCT AAT ...
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bioRxiv - Plant Biology 2024Quote: ... Genomic DNA was extracted from the bacterial strains using the NucleoSpin@ Microbial DNA kit (Macherey-Nagel, Germany). Whole-genome sequence analyses were performed at Bioengineering Lab ...
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bioRxiv - Microbiology 2024Quote: ... SLS was precipitated by acidification and peptides were desalted by using C18 solid phase extraction cartridges (Macherey-Nagel). Cartridges were prepared for sample loading by adding acetonitrile (ACN) ...
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bioRxiv - Microbiology 2024Quote: ... DNA gel extraction kit from Macherey-Nagel (Germany), trizol reagent ...
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bioRxiv - Microbiology 2024Quote: ZIKV RNA was isolated from mouse serum using Nucelospin RNA virus kit (Macherey-Nagel, Düren, Germany), and cDNA was synthesized using ProtoScript II Reverse Transcriptase (New England Biolabs ...
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bioRxiv - Evolutionary Biology 2024Quote: ... we measured the chemical oxygen demand (COD) using NANOCOLOR COD tube tests (detection range 1-15 g/l by Macherey-Nagel (ref: 985 038), see (32 ...
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bioRxiv - Immunology 2024Quote: ... and reverse transcription were performed using the NucleoSpin® RNA plus kit (Macherey-Nagel, 740984.50) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... following the manufacturer’s protocol (Macherey-Nagel, Düren, Germany). DNA quality was evaluated using a Qubit® 3.0 Fluorometer (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2024Quote: ... chondrodes explants using the NucleoSpin® Tissue kit (Macherey-Nagel). We used 15uL qPCR reactions of the Kappa SYBR Fast Ready-Mix (Kappa Biosystems ...
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bioRxiv - Microbiology 2024Quote: ... Initial purification step was realized on a Protino Ni-NTA column (Macherey-Nagel) then on a butyl-Sepharose FF (Cytiva ...
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bioRxiv - Immunology 2024Quote: ... Total RNA was prepared using NucleoSpin RNA Plus Mini kit (Macherey-Nagel) according to manufacturer’s instructions for cDNA synthesis and qPCR as described above.
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bioRxiv - Biochemistry 2024Quote: ... The purified (NucleoSpin® Gel and PCR Clean-up, Macherey-Nagel, Germany) and digested PCR product by NdeI and XhoI restriction enzymes (NEB ...
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bioRxiv - Cancer Biology 2024Quote: Total RNA was extracted from the spleen of 4-6 weeks-old asymptomatic mice using nucleospin RNA II (Macherey-Nagel). The quality of RNA was checked with Bioanalyzer Agilent 2100 and RNAs with a RIN (RNA integrity number ...
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bioRxiv - Bioengineering 2024Quote: ... The PCR product was run on a 1% agarose gel and subsequently purified using the NucleoSpin Gel and PCR Cleanup kit (Macherey-Nagel) according to the manufacturer’s protocol ...
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bioRxiv - Systems Biology 2024Quote: ... PCR products were purified with PCR clean up kit from Macherey-Nagel. Equal molar concentrations of PCR product were mixed and 5 µL of mix was added to 15 µL of Gibson Master mix ...
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bioRxiv - Systems Biology 2024Quote: ... PCR products were purified with PCR clean up kit from Macherey-Nagel. Equal molar concentrations of PCR product were mixed (one from either pMD019 or pMD024 and one from pCRRNA ...
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bioRxiv - Plant Biology 2024Quote: RNA was extracted from 50 mg of frozen and fine ground rosette leaf material from four biological replicates per genotype and growth condition using the NucleoSpin RNA Plant Kit (Macherey-Nagel, Düren, Germany), according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... 1000 ng of total RNAs were isolated using the Nucleospin RNA kit (Macherey-Nagel, 740955) following manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: Total RNA was extracted using Nucleospin RNA kit (Macherey-Nagel, 740955) or RNeasy Plus Micro (Qiagen ...
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bioRxiv - Molecular Biology 2024Quote: ... The rest of the PCRs were purified with the NucleoSpin Gel and PCR Clean-up XS kit (#740611.50, Macherey-Nagel) for long-read amplicon sequencing using Oxford Nanopore Technology (via Plasmidsaurus).
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bioRxiv - Cell Biology 2024Quote: Total RNA extraction was performed using the NucleoSpinRNA kit (Macherey-Nagel) as specified by the manufacturer ...
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bioRxiv - Cell Biology 2024Quote: ... Maxiprep was performed according to the manufacturer’s instructions (NucleoBond® Xtra Maxi Plus EF, Macherey-Nagel), and ...
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bioRxiv - Cancer Biology 2024Quote: ... Genomic DNA (gDNA) was exptracted using the NucleoSpin Blood XL kit (Macherey-Nagel, # 740950.50). The gDNA was amplified according to Broad Genome Perturbation Portal protocols and single end 100 cycle sequencing was performed on a NovaSeq 6000 (Illumina ...
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bioRxiv - Cell Biology 2024Quote: ... RNA was isolated using the NucleoSpin RNA kit (Macherey-Nagel) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... The resulting PCR product was gel purified using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel) and 3’ A-overhangs were added by incubation with Taq-polymerase (DreamTaq Green PCR Master Mix (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2024Quote: ... and constructs were purified using the manufacture’s instruction of the MIDI-Prep system (Macherey-Nagel, #740410.10) The cloned nucleotides were confirmed by sanger sequencing ...
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bioRxiv - Molecular Biology 2024Quote: ... total RNAs were isolated from HeLa and HEK293 cells using the NucleoSpin RNA kit (Macherey-Nagel). To examine endogenous mRNA expression levels and the splicing products ...
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bioRxiv - Molecular Biology 2024Quote: ... DNA was purified with a commercial gel and PCR clean-up kit (Macherey-Nagel, Germany) and was used directly for quantitative PCR with SimpleChIP human CDKN1A promoter primers (Cell Signaling Technology ...
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bioRxiv - Biochemistry 2024Quote: All proteins were purified using Ni-IDA (Macherey-Nagel) following the instructions provided by the manufacturer ...
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bioRxiv - Biochemistry 2024Quote: ... After cell lysis using a French press the cell debris was removed by centrifugation at 17000 g for 1 h at 4 °C and the cleared lysate was incubated with Protino IDA resin (Macherey-Nagel) for 20 min at 4° C ...
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bioRxiv - Biochemistry 2024Quote: ... DNA fragments encoding the open reading frame (ORF) of the nanobodies with 15-bp complementary overhangs were amplified using PCR and purified from agarose gel bands (Macherey-Nagel Ref 740609.50). A pAAV vector ...
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bioRxiv - Biochemistry 2024Quote: ... PCR products for each single cell clone were combined and purified using the NucleoSpin Gel and PCR clean-up kit (Macherey-Nagel). Afterward using a second round of PCR ...
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bioRxiv - Plant Biology 2024Quote: Total RNAs were isolated from the grounded whole seedling using the NucleoSpin RNA Plus Mini kit (Macherey-Nagel, Germany) using the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2024Quote: All chloroform extracts were derivatized (50 μl BSTFA; Macherey-Nagel; and 100 μl pyridine ...