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Citations for Macherey-Nagel GmbH :
551 - 600 of 3263 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: Total RNA from cell lysates was extracted using the NucleoSpin RNA II kit (Macherey-Nagel, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... and purified with the NucleoSpin Gel and PCR Cleanup kit (Macherey-Nagel). The DNA binding reactions were performed in interaction buffer (10 mM Tris-HCl [pH 7.5] ...
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bioRxiv - Systems Biology 2023Quote: ... equipped with a 30-m OPTIMA® 1301-MS column (6% cyanopropylphenyl, 94% dimethylpolysiloxane, 0.25 mm internal diameter, 0.25 µm film thickness; Macherey-Nagel, Düren, Germany). Injections of 1 µL aliquots of sample solution were performed in splitless or split mode depending on the peak intensities (n = 2 ...
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bioRxiv - Immunology 2023Quote: RNAs from whole lungs or BM of naive or IAV-infected mice were extracted and purified using the NucleoSpin RNA Extraction Kit (Macherey-Nagel) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: Total RNA from liver cells was extracted using the NucleoSpin RNA kit (Macherey-Nagel). Viral RNA from cell culture supernatant was isolated using the Quick-RNA Viral kit (ZymoResearch) ...
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bioRxiv - Microbiology 2023Quote: ... The resulting amplicon was purified using the Gel & PCR clean-up kit (Macherey-Nagel, Düren, Germany) and sequenced by Sanger sequencing ...
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bioRxiv - Microbiology 2023Quote: ... and the selection of inactivated cells was performed by DNA extraction using the NucleoSpin Blood kit (Macherey-Nagel) followed by PCR amplification using primers flanking the Cas9 cleavage site ...
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bioRxiv - Microbiology 2023Quote: Whole blood DNA was extracted using the NucleoSpin 96 Blood core kit (Macherey-Nagel, Düren, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: Whole peripheral blood was collected from newly hatched chicks and total DNA was extracted using the NucleoSpin 96 Blood core kit (Macherey-Nagel, Düren, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... and the backbone band was cut out and isolated using NucleoSpin Gel and PCR cleanup Kit (Macherey-Nagel). Backbone and insert were ligated using T4 Ligase (Thermo Fisher ...
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bioRxiv - Biochemistry 2023Quote: ... and plasmid DNA was extracted and purified using the NucleoSpinPlasmid kit (Macherey-Nagel). Bait proteins encoded by pIX-Halo were expressed using the TNT T7-coupled wheat germ extract system (Promega ...
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bioRxiv - Molecular Biology 2023Quote: ... the mCherry+ cells were sorted and total RNA isolated using the NucleoZOL (Macherey-Nagel). Downstream steps were as described for the Ctnnb1 reporter ...
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bioRxiv - Molecular Biology 2023Quote: ... mCherry+ cells were sorted 48 hr later and their total RNA isolated using NucleoZOL (Macherey-Nagel). The mRNA fraction was then enriched using a NEBNext Poly(A ...
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bioRxiv - Microbiology 2023Quote: ... Purification was carried out using the RNA Clean-Up Kit (Macherey-Nagel). Finally ...
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bioRxiv - Microbiology 2023Quote: ... Samples were treated with rDNase (Macherey-Nagel, Düren, Germany) according to the manufacturer’s protocol to minimize DNA contamination ...
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bioRxiv - Microbiology 2023Quote: ... and DMSO as a control at 28°C and 150 rpm using a NucleoSpin RNA Plant and Fungi kit (Macherey-Nagel) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2023Quote: ... RNA was extracted using the NucleoSpin RNA Plant and Fungi kit (Macherey-Nagel) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2023Quote: ... RNA was harvested using the NucleoSpin RNA Virus kit (MACHEREY-NAGEL, 740956) following manufacturer’s instructions and reverse-transcribed with SuperScript IV (Invitrogen ...
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bioRxiv - Plant Biology 2023Quote: ... performed with the NucleoSpin Plasmid kit (Macherey-Nagel, Germany); the successful cloning was verified by Sanger sequencing.
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bioRxiv - Cell Biology 2023Quote: ... Correct products were isolated with gel electrophoresis and gel extraction (Macherey-Nagel, F40609.250), and were ligated with T4 DNA ligase (Thermo Fisher ...
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bioRxiv - Microbiology 2023Quote: ... Genomic DNA and mRNA were extracted using NucleoSpin Tissue and NucleoSpin RNA kits (Macherey-Nagel), respectively.
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bioRxiv - Biochemistry 2023Quote: ... 2 μL sample volume was injected into a Nucleodur HILIC column (250 mm * 2 mm, 3 μm particle size, Macherey-Nagel). Metabolites were eluted at 20 °C with an acetonitrile gradient (90% (v/v ...
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bioRxiv - Microbiology 2023Quote: ... The dephosphorylated pEX18Tc vector and PCR products were purified using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel, Düren, Germany). TEDA reaction was then carried out by mixing 150 ng of pEX18Tc with the corresponding PCR products at a molar ratio of 1:4 ...
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bioRxiv - Microbiology 2023Quote: ... Plasmids (pEX18Tc-Δhcp) were extracted with the NucleoSpin plasmid kit (Macherey-Nagel, Düren, Germany), and insertion regions were verified by sequencing (Azenta Life Sciences ...
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bioRxiv - Microbiology 2023Quote: ... inoculated seeds and seedlings of the SynCom conditions (control, 6-strain, 8-strain, 12-strain SynComs) with the NucleoSpin® 96 Food kit (Macherey-Nagel, Düren, Germany) following the manufacturer’s instructions.
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bioRxiv - Systems Biology 2023Quote: ... Total RNA was isolated using the NucleoSpin RNA II kit (Macherey-Nagel). The amount of RNA was determined using a NanoDrop spectrophotometer (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... or the NucleoBond Xtra Midi EF kit (MACHEREY-NAGEL). Correct inserts were confirmed by sequencing (Genewiz).
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bioRxiv - Cell Biology 2023Quote: ... or NucleoBond Xtra Midi EF kit (MACHEREY-NAGEL).
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bioRxiv - Plant Biology 2023Quote: ... floral organs and fruits using the “NucleoSpin RNA Plant” kit (Macherey-Nagel). After DNase I treatment ...
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bioRxiv - Cell Biology 2023Quote: ... RNAs were extracted using NucleoSpin RNA kit (Macherey-Nagel, Düren, Germany). Then ...
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bioRxiv - Microbiology 2023Quote: ... RNA was isolated and purified from cells using the NucleoSpin RNA extraction kit (Macherey-Nagel). Following RNA isolation ...
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bioRxiv - Genomics 2023Quote: DNA extractions were carried out using the NucleoSpin Plant II kit (Macherey-Nagel, GmbH, Germany) following the manufacturer’s protocol ...
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bioRxiv - Genetics 2023Quote: ... Genomic DNA was isolated from pelleted cells by column purification (Macherey-Nagel, 740950.50), and the sgRNA expression cassette was amplified by 22 cycles of PCR using NEBNext Ultra II Q5 Master Mix and primers containing Illumina P5/P7 termini and sample-specific TruSeq indices ...
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bioRxiv - Plant Biology 2023Quote: ... 6His-AtREM1.21–118 was produced like GST-CPK and purified on Protino® Ni-TED column following manufacturer’s instructions (Macherey-Nagel). After elution with 40-250 mM imidazole ...
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bioRxiv - Plant Biology 2023Quote: RNA isolation was performed by using the NucleoSpin® RNA kit (Macherey-Nagel, Düren, Germany) following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... The mixture was purified using Nucleospin® Gel and PCR clean-up kit (Macherey-Nagel # 740609), quantified by Qubit (Thermo Fisher) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Extraction of genomic DNA was performed using the NucleoSpin@ Tissue kit by Macherey-Nagel, following the manufacturer’s protocol ...
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A randomized multiplex CRISPRi-Seq approach for the identification of critical combinations of genesbioRxiv - Genetics 2023Quote: ... All colonies were scraped off plates using an inoculation loop and added directly to the A1 buffer of a Nucleospin Plasmid Purification Kit (Macherey-Nagel 740499.250) for plasmid isolation and elution in water ...
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bioRxiv - Microbiology 2023Quote: Amplicons were purified with the NucleoSpin kit (MACHEREY-NAGEL), sheared in a Bioruptor Pico (Diagenode ...
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A randomized multiplex CRISPRi-Seq approach for the identification of critical combinations of genesbioRxiv - Genetics 2023Quote: ... The reaction was purified using a NucleoSpin PCR Clean-up Kit (Macherey-Nagel 740609.250) and eluted in 30 µL 1X TE buffer.
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A randomized multiplex CRISPRi-Seq approach for the identification of critical combinations of genesbioRxiv - Genetics 2023Quote: ... All colonies were scraped off plates using an inoculation loop and added directly to the A1 buffer of a Nucleospin Plasmid Purification Kit (Macherey-Nagel 740499.250) for plasmid isolation and elution in 20 µL 1X TE buffer ...
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A randomized multiplex CRISPRi-Seq approach for the identification of critical combinations of genesbioRxiv - Genetics 2023Quote: ... bacteria from the cultures used for infection were immediately pelleted by centrifugation and resuspended in 8 mL of RES-EF of the NucleoBond Xtra Midi EF kit prior to proceeding with the standard kit protocol (Macherey-Nagel 740420.50). Precipitated vectors were resuspended in 120 µL of water.
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A randomized multiplex CRISPRi-Seq approach for the identification of critical combinations of genesbioRxiv - Genetics 2023Quote: ... and we collected bands containing arrays ≥2-plex at 550-650 bps and 650-800 bps to be purified separately using a NucleoSpin Gel Purification Kit (Macherey-Nagel 740609.250). We also collected the cut vector backbone ...
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A randomized multiplex CRISPRi-Seq approach for the identification of critical combinations of genesbioRxiv - Genetics 2023Quote: ... were scraped off plates using an inoculation loop and added directly to the A1 buffer of a Nucleospin Plasmid Purification Kit (Macherey-Nagel 740499.250) for plasmid isolation and elution in 20 µL water ...
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bioRxiv - Developmental Biology 2023Quote: Total RNAs from cultured human iPS cells were extracted using NucleoSpin RNA Plus XS (Macherey-Nagel). The 100 ng of total RNAs were used as starting materials to generate RNA-seq libraries with the TruSeq Stranded mRNA LT sample prep kit (Illumina) ...
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bioRxiv - Bioengineering 2023Quote: ... samples were filtered through 0.45 µm GF/PET filters (Chromafil, Macherey-Nagel). Ion chromatography (881 compact IC pro ...
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bioRxiv - Biochemistry 2023Quote: Propagated plasmids were purified using NucleoSpin® Plasmid from Macherey-Nagel, MN (Germany ...
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bioRxiv - Genomics 2023Quote: ... DNA and RNA were extracted from the powder using NucleoSpin TriPrep mini kit columns (Macherey-Nagel), according to the manufacturer’s main instructions but with a few modifications ...
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bioRxiv - Systems Biology 2023Quote: Total RNA was isolated using NucleoSpin RNA Plus (Macherey-Nagel, Duren, Germany, Cat. No. 740984.250). Library construction and RNALseq were performed using the Illumina sequencing platform (Rhelixa) ...
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bioRxiv - Microbiology 2023Quote: ... and DNA bands of interest were isolated using the NucleoSpin® Gel & PCR Cleanup Kit from Macherey-Nagel. The extracted DNA underwent Sanger sequencing at Eurofins Scientific ...