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Citations for Macherey-Nagel GmbH :
351 - 400 of 3263 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2024Quote: 200,000 cells were collected on day 14 after crRNA transduction and genomic DNA was isolated using NucleoSpin Blood (Macherey-Nagel, Catalog no. 740951.50). Briefly ...
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bioRxiv - Genetics 2024Quote: ... PCRs for loci of interest were run using Amplicon-EZ (Genewiz) partial IlluminaÒ adapters and amplicons were processed using NucleoSpin Gel and PCR Clean-up Kit (Macherey-Nagel, Catalog no. 740609.250). Paired end (2 x 250 bp ...
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bioRxiv - Cell Biology 2024Quote: Isolation of total RNA was performed using the NucleoSpin RNA II kit (Macherey-Nagel, cat. # 740955.50) following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: Total RNAs were extracted from 100 mg of tissue with the Nucleospin RNA plant (Macherey-Nagel) kit following the manufacturer instructions ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: RNA purification and reverse transcription were performed using the Nucleospin RNA kit from Macherey-Nagel (Düren, Germany) and the High capacity cDNA reverse transcription kit from Applied Biosystems (Woolston ...
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bioRxiv - Plant Biology 2024Quote: ... total RNA was extracted from plants grown on plates using NucleoSpin RNA extraction kit (Macherey-Nagel GmbH & Co.). First-strand cDNA was synthesized using 2 μg RNA and PrimeScript RT Master Mix (Takara Bio) ...
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bioRxiv - Microbiology 2024Quote: ... The last purification step was performed with the RNA Nucleospin kit according to manufacturer’s instructions (Macherey-Nagel). The integrity of the RNAs was verified using the Agilent 2100 bioanalyzer with the RNA 6000 Nano kit (Agilent Technologies) ...
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bioRxiv - Biophysics 2024Quote: ... The lysate was clarified by centrifugation (7197g for 30 min) and applied twice at 4 °C to a Protino Ni-IDA 2000 column (Macherey-Nagel), pre-equilibrated with IMAC buffer containing 50 mM sodium phosphate buffer pH 8.0 and 300mM NaCl ...
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bioRxiv - Biophysics 2024Quote: ... A 2-μl aliquot of each sample was spotted on a TLC sheet (SIL/UV254, Macherey-Nagel), and the sheet developed in a mixture of n-butanol ...
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bioRxiv - Cancer Biology 2024Quote: ... Total RNA was isolated using NucleoSpin RNA Plus (Macherey-Nagel, Duren, Germany, Cat. No. 740984.250) at the respective specified time ...
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bioRxiv - Cell Biology 2024Quote: RNA was isolated from the interphase of extracted cells as described before [20] by using the NucleoSpin® RNA kit (MACHEREY-NAGEL, 740955.50). Isolated RNA was converted to cDNA by using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems™ ...
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bioRxiv - Microbiology 2024Quote: RNA was extracted from parasites using NuceloSpin RNA kit (Macherey-Nagel). cDNA was obtained using SuperScript III First Strand Synthesis SuperMix for RT-qPCR (Invitrogen ...
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bioRxiv - Physiology 2024Quote: ... RNA was extracted from the homogenised pallium samples using the NucleoSpin RNA Plus kit (MACHEREY-NAGEL). Since the sample size of the control group was the main factor limiting statistical power ...
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bioRxiv - Biochemistry 2024Quote: ... and 51μm filters using Macherey-Nagel a NucleoMag RNA kit (Macherey-Nagel GmbH & Co.KG). Cleared lysate was loaded into a 96 deep-well plate and put on an epMotion 5075 TMX liquid handler to complete the RNA extraction following the Machery-Nagel standard protocol ...
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bioRxiv - Cell Biology 2024Quote: ... RNA was extracted using NucleoSpin® RNA kit (Macherey-Nagel, Bethlehem, PA, USA). RNA quality and quantity were assessed using Nanodrop-2000 (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... Plasmid preparation and DNA gel extraction were performed using the Nucleospin Plasmid and the Nucleospin Extract II kits (Macherey-Nagel, Lab Supplies Scientific SA, Hellas). Restriction enzymes were from Takara Bio (Lab Supplies Scientific SA ...
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bioRxiv - Evolutionary Biology 2024Quote: ... crushed the samples using five MN Beads Type D (Macherey-Nagel). We crushed leg samples with only three beads ...
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bioRxiv - Evolutionary Biology 2024Quote: ... We extracted total genomic DNA from each whole individual sample using NucleoMag® Tissue Kit (Macherey-Nagel) according to the manufacturer’s recommendations ...
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bioRxiv - Genomics 2024Quote: ... from which HMW DNA was isolated by NucleoBond HMW DNA kit (Macherey-Nagel), following the manufacturer’s protocol ...
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bioRxiv - Evolutionary Biology 2024Quote: ... or NucleoSpin® Tissue Kit (Macherey-Nagel, Düren, Germany). Otoliths were fully submerged in the lysis buffer to lysate any remnant tissues then removed from the buffer ...
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bioRxiv - Cell Biology 2024Quote: ... RNA was extracted using a modified protocol of the Nucleospin 96 RNA kit (Macherey-Nagel, Duren, Germany). Cells were lysed in a 96 deep-well plate by adding 450μl of lysis buffer containing 1M sorbitol (Sigma-Aldrich) ...
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Assessment of the transcriptional regulation of EMT and MET through accessible transposable elementsbioRxiv - Genomics 2024Quote: Isolation of RNA samples was performed with the Nucleospin RNA II kit (Macherey-Nagel) as recommended by the manufacturer ...
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bioRxiv - Evolutionary Biology 2024Quote: ... we measured the chemical oxygen demand (COD) using NANOCOLOR COD tube tests (detection range 1-15 g/l by Macherey-Nagel (ref: 985 038), see (32 ...
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bioRxiv - Immunology 2024Quote: ... and reverse transcription were performed using the NucleoSpin® RNA plus kit (Macherey-Nagel, 740984.50) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... following the manufacturer’s protocol (Macherey-Nagel, Düren, Germany). DNA quality was evaluated using a Qubit® 3.0 Fluorometer (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2024Quote: ... chondrodes explants using the NucleoSpin® Tissue kit (Macherey-Nagel). We used 15uL qPCR reactions of the Kappa SYBR Fast Ready-Mix (Kappa Biosystems ...
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bioRxiv - Microbiology 2024Quote: ... Initial purification step was realized on a Protino Ni-NTA column (Macherey-Nagel) then on a butyl-Sepharose FF (Cytiva ...
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bioRxiv - Immunology 2024Quote: ... Total RNA was prepared using NucleoSpin RNA Plus Mini kit (Macherey-Nagel) according to manufacturer’s instructions for cDNA synthesis and qPCR as described above.
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bioRxiv - Biochemistry 2024Quote: ... The purified (NucleoSpin® Gel and PCR Clean-up, Macherey-Nagel, Germany) and digested PCR product by NdeI and XhoI restriction enzymes (NEB ...
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bioRxiv - Cancer Biology 2024Quote: Total RNA was extracted from the spleen of 4-6 weeks-old asymptomatic mice using nucleospin RNA II (Macherey-Nagel). The quality of RNA was checked with Bioanalyzer Agilent 2100 and RNAs with a RIN (RNA integrity number ...
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bioRxiv - Bioengineering 2024Quote: ... The PCR product was run on a 1% agarose gel and subsequently purified using the NucleoSpin Gel and PCR Cleanup kit (Macherey-Nagel) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: Total RNA extraction was performed using the NucleoSpinRNA kit (Macherey-Nagel) as specified by the manufacturer ...
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bioRxiv - Cell Biology 2024Quote: ... Maxiprep was performed according to the manufacturer’s instructions (NucleoBond® Xtra Maxi Plus EF, Macherey-Nagel), and ...
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bioRxiv - Cell Biology 2024Quote: ... Nucleospin Gel and PCR clean-up kit (Macherey-Nagel) was employed to purify DNA from the gel ...
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bioRxiv - Microbiology 2024Quote: ... was performed either when all ammonium (or nitrate) and nitrite was depleted, or when the values stayed constant for 1-3 days (qualitatively, using Quantofix (Macherey-Nagel) strips) ...
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bioRxiv - Biochemistry 2024Quote: ... after which positive colonies were purified using a Nucleospin Plasmid miniprep kit (Macherey-Nagel) and sent for Sanger Sequencing on an Applied Biosystems 3730XL DNA Analyzer (Neuromics Support Facility ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... RNA extraction was carried out with the NucleoSpin RNA kit (Macherey-Nagel, #740955) following the guidelines provided by the manufacturer ...
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bioRxiv - Molecular Biology 2024Quote: ... The rest of the PCRs were purified with the NucleoSpin Gel and PCR Clean-up XS kit (#740611.50, Macherey-Nagel) for long-read amplicon sequencing using Oxford Nanopore Technology (via Plasmidsaurus).
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bioRxiv - Molecular Biology 2024Quote: ... total RNAs were isolated from HeLa and HEK293 cells using the NucleoSpin RNA kit (Macherey-Nagel). To examine endogenous mRNA expression levels and the splicing products ...
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bioRxiv - Cell Biology 2024Quote: ... RNA was isolated using the NucleoSpin RNA kit (Macherey-Nagel) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... The resulting PCR product was gel purified using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel) and 3’ A-overhangs were added by incubation with Taq-polymerase (DreamTaq Green PCR Master Mix (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2024Quote: RNA was isolated from cells using NucleoSpin RNA Plus (Macherey-Nagel, Duren, Germany, Cat. No. 740984.250) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... and DNA was extracted using Nucleospin Gel and PCR Clean-up kit (Macherey-Nagel Cat. 740609) and cleaned with QIAGEN MinElute PCR purification kit (QIAGEN cat ...
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bioRxiv - Cancer Biology 2024Quote: ... Genomic DNA (gDNA) was exptracted using the NucleoSpin Blood XL kit (Macherey-Nagel, # 740950.50). The gDNA was amplified according to Broad Genome Perturbation Portal protocols and single end 100 cycle sequencing was performed on a NovaSeq 6000 (Illumina ...
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bioRxiv - Cancer Biology 2024Quote: ... and constructs were purified using the manufacture’s instruction of the MIDI-Prep system (Macherey-Nagel, #740410.10) The cloned nucleotides were confirmed by sanger sequencing ...
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bioRxiv - Molecular Biology 2024Quote: ... DNA was purified with a commercial gel and PCR clean-up kit (Macherey-Nagel, Germany) and was used directly for quantitative PCR with SimpleChIP human CDKN1A promoter primers (Cell Signaling Technology ...
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bioRxiv - Cancer Biology 2024Quote: ... The RNA of the sorted cells was extracted using a Nucleospin RNA XS kit (Macherey-Nagel), and its concentration was measured using QuBit ...
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bioRxiv - Cancer Biology 2024Quote: Total RNAs were extracted using nucleospin RNA II (Macherey-Nagel), reverse-transcribed using superscript IV (Invitrogen) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... DNA extractions were either performed with a modified CTAB 2%/PVPP 2% protocol (see Jolly et al. 2003) or the NucleoSpin®Tissue 96 kit (Macherey-Nagel, Karlsruhe, Germany).
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bioRxiv - Cell Biology 2024Quote: ... Plasmids were purified using a maxiprep kit (Macherey-Nagel, #740574.25) and sequenced ...