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Citations for Macherey-Nagel GmbH :
201 - 250 of 3263 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: ... PCR product was then purified by following NucleoSpin PCR Clean-up procedure (Macherey-Nagel; Cat# 740609.250) and sent for Sanger sequencing to confirm genotypes.
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bioRxiv - Microbiology 2024Quote: ... DNA fragments and PCR products were purified using the Nucleospin Gel and PCR clean up kit (Macherey-Nagel).
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bioRxiv - Microbiology 2024Quote: ... The PCR amplicons were gel purified with NucleoSpin gel and PCR clean-up kits (Macherey-Nagel, Germany) following the manufacturer’s protocol ...
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bioRxiv - Genetics 2024Quote: ... Maxi kit (Macherey-Nagel). Cell culture was performed whenever the cells had grownto 90% of the cell plate.
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bioRxiv - Genetics 2024Quote: ... the plasmid library was obtained using NucleoBond Xtra Midi EF kit (Macherey-Nagel). The oligo library was confirmed using nested PCR and Illumina sequencing.
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bioRxiv - Evolutionary Biology 2024Quote: ... Peptides were desalted by using C18 solid phase extraction cartridges (Macherey-Nagel). Cartridges were prepared by adding acetonitrile (ACN) ...
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bioRxiv - Genomics 2024Quote: ... dissolved in TE buffer (10 mM Tris pH8, 1 mM EDTA pH8) and purified using NucleoSpin Gel and PCR clean-up (Macherey-Nagel). DNA was loaded on a 2% agarose gel ...
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bioRxiv - Genomics 2024Quote: ... Cross-linking was reversed by overnight incubation at 65°C and DNA fragments were purified using NucleoSpin Gel and PCR Clean-up columns (Macherey-Nagel).
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bioRxiv - Evolutionary Biology 2024Quote: ... genomic DNA extractions were performed using the NucleoSpin Microbial DNA Mini kit (Macherey-Nagel) following manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and the NucleoBond HMW DNA kit (Macherey-Nagel, North Rhine-Westphalia, Germany) eluted in 100 µl–200 µl low-pH TE buffer ...
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bioRxiv - Evolutionary Biology 2024Quote: ... PCR amplified samples were purified using the NucleoSpin Gel and PCR cleanup kit (Macherey-Nagel) and quantified on the NanoDrop 2000 spectrophotometer (Thermo Scientific) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... using whole animal total RNA extracted from approximately 30 animals using a Nucleospin RNA Plus Mini Kit (Macherey-Nagel, Düren, Germany), both according to manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: RNA was extracted from mouse lungs using the Nucleospin 96 RNA extraction kit as per written instructions (Macherey-Nagel 740709.4). Prior to being run through the binding columns ...
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bioRxiv - Genetics 2024Quote: ... DNAs were extracted using NucleoSpin Blood XL (Macherey-Nagel#740950). Samples were prepared for sequencing as previously described35.
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bioRxiv - Genomics 2024Quote: DNA was extracted using either the OmniPrep Genomic DNA Purification Kit (G Biosciences, St. Louis, MO, USA) or the Nucleospin Plant II midi DNA Extraction Kit (Macherey-Nagel, Düren, Germany). DNA quality was assessed using a Qubit fluorometer (Themo Fisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... Total RNA was extracted from frozen powder with the NucleoSpin® RNA kit (Macherey-Nagel, 740955). RNA samples were concentrated with NucleoSpin® RNA Clean-up XS (Macherey-Nagel ...
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bioRxiv - Genetics 2024Quote: ... while the ZB5d/antibiotics mixture was further processed with the NucleoMag VET Kit (Macherey-Nagel, Düren, Germany) in a final elution volume of 100µL VEL-buffer using a KingFisher®Flex automat (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2024Quote: ... Resulting PCR products were purified with the Bioanalysis NucleoSEQ Kit (Macherey-Nagel), and 15 µL of each eluate was mixed with the same volume of Hi-Di formamide (Thermo Fisher Scientific) ...
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bioRxiv - Genetics 2024Quote: Total RNA from twenty heads of 3 day-old Drosophila adults for each genetic combination was purified using the RNA isolation kit of Macherey-Nagel (Macherey-Nagel, Düren, Germany) according to the manufacturer’s instructions.1 μg of total RNA was reverse transcribed using the High-Capacity cDNA Archive Kit (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2024Quote: ... RNA & Protein Purification kit (Macherey-Nagel). Clones were screened by XhoI digestion and Sanger sequencing.
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bioRxiv - Immunology 2024Quote: ... RNA samples were concentrated with NucleoSpin® RNA Clean-up XS (Macherey-Nagel, 740903) or GeneJet RNA Cleanup and Concentration Micro Kit (Life Technologies ...
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bioRxiv - Genomics 2024Quote: ... each side of the Amicon filter was rinsed and incubated at 56°C for 1 hour (per side) in 200 µL Buffer T1 and 20 µL Proteinase K from the NucleoSpinl1J Tissue Kit (MACHEREY-NAGEL Inc., Allentown, PA, United States) and DNA extraction proceeded from step 3 of the kit ...
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bioRxiv - Microbiology 2024Quote: PCR products were purified using NucleoSpin Gel and PCR Clean-up (Macherey-Nagel) according to manufacturer’s recommendations ...
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bioRxiv - Microbiology 2024Quote: ... PCR clean-up (Macherey-Nagel Cat. No. 740609.250S), and Sanger sequencing (Microsynth GmbH ...
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bioRxiv - Microbiology 2024Quote: Total genomic DNA was extracted using the NucleoMag® Tissue extraction kit (Macherey-Nagel™, Düren, Germany) and the V3-V4 region of the 16S rDNA gene was amplified with the prokaryotic primers 343F (5′-ACGGRAGGCAGCAG – 3′ ...
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bioRxiv - Microbiology 2024Quote: ... PCRs were purified using NucleoSpin® Gel and PCR Clean-up kit (Macherey-Nagel). Plasmids and PCRs were digested with the appropriate restriction enzymes (New England Biolabs) ...
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bioRxiv - Microbiology 2024Quote: ... RNA was column purified using NTC buffer and the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel), according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2024Quote: Large and small (including miRNAs) RNA fractions were isolated from the same macrophage samples using the NucleoSpin miRNA kit (Macherey-Nagel) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... RNA was then extracted using Nucleospin RNA kit (Macherey-Nagel 740955) following manufacturer instructions ...
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bioRxiv - Microbiology 2024Quote: ... Total DNA and RNA was extracted using the NucleoMag VET extraction kit (Macherey-Nagel, Germany) as described by the manufacturer’s instructions with the IDEALTM 96 extraction robot (Innovative Diagnostics ...
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bioRxiv - Microbiology 2024Quote: ... DNA was extracted using 100 µL of homogenate with the Genomic DNA tissue kit (Macherey-Nagel, Germany). Detection and quantification of R ...
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bioRxiv - Immunology 2024Quote: Serum samples from all experimental groups were extracted using the NucleoSpin RNA isolation kit (Macherey-Nagel, Allentown, PA). Following viral RNA extraction ...
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bioRxiv - Microbiology 2024Quote: ... The PCR products were purified using NucleoSpin Gel and PCR Cleanup Kit (Macherey-Nagel), following the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: Total RNA was extracted using NucleoSpin RNA kit (Macherey-Nagel) and cDNA was synthesized with the SuperScript III First-Strand Synthesis System for RT-PCR (Thermo Fischer Scientific) ...
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bioRxiv - Immunology 2024Quote: RNA samples were isolated using the NucleoSpin RNA extraction kit (Macherey-Nagel, Cat. No. 740955) from mock transfected and ANKRD55-transfected cells at 40 hr after transfection ...
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bioRxiv - Immunology 2024Quote: All samples were directly transferred to 700 µl of RA1 lysis buffer (Macherey-Nagel, Düren, Germany, cat. no. 740961) containing 1% β-mercaptoethanol ...
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bioRxiv - Immunology 2024Quote: ... Total RNA was extracted from the lysates using the NucleoMag Vet kit (Macherey-Nagel, cat.no. 744200) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2024Quote: High-molecular-weight DNA was extracted with the NucleoBond® HMW DNA kit (Macherey-Nagel Gmbh & Co, Germany) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: Total RNA was isolated from cell samples using the NucleoSpin RNA XS Kit (Macherey-Nagel) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... Supernatant was incubated with Protino Glutathione Agarose 4B beads (Macherey-Nagel, 745500) in a rotatory shaker overnight at +4°C ...
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bioRxiv - Immunology 2024Quote: ... Mini kit (Macherey-Nagel). RNA quality and quantity were analyzed by NanoDrop 1000 (PEQLab ...
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bioRxiv - Immunology 2024Quote: ... Total RNA was isolated using the Nucleospin RNA mini kit (Macherey-Nagel) following manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... The CBP-His6-ZZ-Upf1-HD proteins were first purified on a Protino® Ni-NTA agarose resin (Macherey-Nagel). After successive washing steps using buffers C200 first ...
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bioRxiv - Biochemistry 2024Quote: ... Ni-IDA affinity beads (Macherey-Nagel, Bethlehem, PA, USA) were used to purify TBC-DEG assemblies ...
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bioRxiv - Biochemistry 2024Quote: Products formed in in vitro or whole-cell reactions were quantified via GC-FID using a non-chiral OPTIMA™ 5MS column (Macherey-Nagel GmbH & Co. KG, Düren, Germany). Substrates and products were directly extracted from the aqueous reaction solutions with DCM containing 2 mM acetophenone as an internal standard ...
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bioRxiv - Biochemistry 2024Quote: ... Plasmid preparation kits were purchased from Macherey-Nagel.
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bioRxiv - Biochemistry 2024Quote: ... 4°C and the supernatant was incubated at 4 °C with 1.25 ml Ni-NTA agarose resin (Macherey-Nagel) for 1 h with agitation ...
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bioRxiv - Biochemistry 2024Quote: ... Clarified lysates were filtered (0.45 µm syringe tip filters) and applied to pre-equilibrated Protino Ni-NTA Agarose (Macherey-Nagel) beats in a gravety column ...
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bioRxiv - Bioengineering 2024Quote: ... The acquired plasmid was purified using NucleoSpin Plasmid EasyPure (Macherey-Nagel) kit according to suppliers instructions ...
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bioRxiv - Bioengineering 2024Quote: ... were gel purified using NucleoSpin Gel and PCR Clean-up (Macherey-Nagel) kit according to suppliers’ instructions ...