Labshake search
Citations for Macherey-Nagel GmbH :
201 - 250 of 3300 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
Targeted Perturb-seq Reveals EGR1 and FOS as Key Regulators of the Transcriptional RAF-MAPK ResponsebioRxiv - Systems Biology 2024Quote: ... The digestion reaction was run on a 1% agarose gel followed by excision of the digested band and purification via NucleoSpin columns (Macherey-Nagel). 100 ng digested pMB1-10x and 2.4 ng amplified sgRNA library insert were assembled using Gibson Assembly Master Mix (NEB ...
-
bioRxiv - Systems Biology 2024Quote: ... Total worm RNA was extracted by using a column-based kit from Macherey-Nagel. RNA-seq was performed by BGI with the BGISEQ-500 platform ...
-
bioRxiv - Plant Biology 2024Quote: ... The samples were pulverized in liquid nitrogen and RNA was extracted using the NucleoZOL reagent (MACHEREY-NAGEL, Germany), following the manufacturer’s instructions ...
-
bioRxiv - Synthetic Biology 2024Quote: ... Spheroplasts were collected by centrifugation and RNA was isolated using the NucleoSpin RNA Plus kit (Macherey-Nagel). RNA quality and integrity was determined by Qubit fluorometry using an RNA BR Assay Kit (ThermoFisher) ...
-
bioRxiv - Molecular Biology 2024Quote: ... 7.5% β-mercaptoethanol and 10 μg/mL RNaseA (Macherey-Nagel)) and incubated for 1 h at 37°C ...
-
bioRxiv - Neuroscience 2024Quote: ... Peptides were then desalted and concentrated using Chromabond C18WP spin columns (Macherey-Nagel, Part No. 730522). Finally ...
-
bioRxiv - Neuroscience 2024Quote: ... genomic DNA was isolated using a Macherey-Nagel Nucleospin Blood kit (Macherey-Nagel; Cat. No. 740951.50) or Macherey-Nagel Nucleospin Blood L kit (Macherey-Nagel ...
-
bioRxiv - Genetics 2024Quote: BAC DNA was extracted from BAC clones (BacPac Resources, CHORI, Oakland) using the Nucleobond Xtra BAC kit (Macherey-Nagel) and subsequently labeled (Nick translation protocol ...
-
bioRxiv - Plant Biology 2024Quote: ... PCR products were purified using the NucleoSpin Gel and PCR Cleanl1Jup kit (MACHEREY-NAGEL) and introduced into linearized (with KpnI ...
-
bioRxiv - Plant Biology 2024Quote: Leaf total RNA was extracted using the NucleoZOL protocol (Macherey-Nagel) followed by RNA purification using Agencourt RNA Clean XP beads (Beckman-Coulter) ...
-
bioRxiv - Biochemistry 2024Quote: ... pcDNA5/FRT-SUGCT and pOG44 plasmid DNA was purified by NucleoBond Xtra Midi Plus EF DNA purification Kit (Macherey-Nagel). Purified pOG44 and pcDNA5/FRT-SUGCT plasmids were co-transfected into Flp-In 293 cells at a 9:1 ratio by using Lipofectamine 2000 reagent (Invitrogen ...
-
bioRxiv - Biochemistry 2024Quote: ... SSC was separated on a 250 x 3 mm 5 µm Nucleodur HTec C18 (Macherey-Nagel) column ...
-
bioRxiv - Cell Biology 2024Quote: ... Total RNA was extracted from the freezed samples using NucleoSpin RNA kit (Macherey-Nagel) and concentration of extracted RNA was measured with Nanodrop spectrophotometer at 260 nm ...
-
bioRxiv - Bioengineering 2024Quote: ... The constructs were assembled into the AAV2-ITR plasmid via molecular cloning and purified using the NucleoBond Xtra Maxi EF kit (Macherey-Nagel, Düren, Germany). The purified plasmids were sent to Vigene Biosciences (now Charles River ...
-
bioRxiv - Bioengineering 2024Quote: ... PCR products from genomic DNA and cDNA were purified using NucleoSpin Gel and PCR Clean-up Mini Kit (Macherey-Nagel, Allentown, PA, USA), and quality was validated via agarose gel electrophoresis.
-
bioRxiv - Plant Biology 2024Quote: ... total RNA was extracted from approximately 100 mg of ground tissue using the NucleoSpin RNA Plant Kit (Macherey-Nagel) and treated with DNase I on the column ...
-
bioRxiv - Plant Biology 2024Quote: ... mRNA was extracted using the NucleoSpin RNA Plant Kit (Macherey-Nagel 740949) modified to increase first RA3 buffer wash to 650 µl and an additional 400 µl RA3 buffer wash ...
-
bioRxiv - Plant Biology 2024Quote: ... and subjected to genomic DNA extraction using the Nucleo Mag Plant kit (Macherey-Nagel GmbH &Co. KG, Germany) in the automated nucleic acid extractor Maelstrom 4800 (TANBead Taiwan Advanced Nanotech Inc. ...
-
bioRxiv - Plant Biology 2024Quote: ... the PCR products were purified using a NucleoSpin® Gel and PCR Clean-up kit (Macherey-Nagel). Sequencing reactions were made up of 20-25/ng of the purified PCR product ...
-
bioRxiv - Physiology 2024Quote: Lung tissue total RNA was extracted in NucleoZOL (740404.200; Macherey-Nagel, German). The quality and integrity of extracted RNA were evaluated using Nanodrop Specthophotometer ...
-
bioRxiv - Plant Biology 2024Quote: ... RNA was extracted using the NucleoSpin® RNA Plant kit (Macherey-Nagel) according to the manufacturer’s instructions and quantified with a Nanodrop spectrophotometer ...
-
bioRxiv - Plant Biology 2024Quote: RNA extraction was performed with NucleoSpin® RNA plus kit (Macherey-Nagel). RNA extraction was performed with leaves from 28-days-old healthy plants (6 plants ...
-
bioRxiv - Plant Biology 2024Quote: ... The soluble protein was first purified by affinity chromatography with Protino nickel nitrilotriacetic acid agarose (Macherey-Nagel) using the lysis buffer but with the imidazole concentration changed to sequential rounds of 20 mM and 250 mM for washing and eluting the protein ...
-
bioRxiv - Plant Biology 2024Quote: RNA was isolated by NucleoSpin RNA Plant Kit (Macherey-Nagel 1806/001) according to manufacturer’s instruction ...
-
bioRxiv - Plant Biology 2024Quote: ... Total RNA was isolated using the NucleoSpin RNA Plus Mini kit (Macherey-Nagel, Hoerdt, France) following manufacturer’s recommendations.
-
bioRxiv - Physiology 2024Quote: ... mRNA from CD11B+ cells was extracted using an RNA XS Plus extraction kit (Macherey-Nagel®).
-
bioRxiv - Plant Biology 2024Quote: ... The protein was purified using Protino Ni-NTA agarose (Macherey-Nagel, #7450400-500).
-
bioRxiv - Genetics 2024Quote: ... Genomic DNA was isolated using NucleoBond HMW DNA (MACHEREY-NAGEL) according to the manufacturer’s protocol.
-
bioRxiv - Animal Behavior and Cognition 2024Quote: ... and extracted RNA using the Nucleospin miRNA kit (Macherey-Nagel; Duren, Germany), which extracts both large RNA (>200 bp ...
-
bioRxiv - Microbiology 2024Quote: ... Total RNA for quantitative reverse transcription PCR (qRT-PCR) was isolated using the NucleoSpin® RNA Kit (Macherey-Nagel), including DNA digestion ...
-
bioRxiv - Cell Biology 2024Quote: ... RNA was isolated using the NucleoSpin RNA (Macherey-Nagel #740955) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2024Quote: ... PO4 concentrations were analysed after filtering extraction solution (Mn 619 G1/4, Macherey-Nagel) using continuous flow analyser (Seal Analytical ...
-
bioRxiv - Microbiology 2024Quote: ... PCR products for assembly were purified using the NucleoSpin Gel and PCR Clean- up Kit (Macherey-Nagel), and plasmids from positive clones were extracted using the NucleoSpin Plasmid Kit (Macherey-Nagel) ...
-
bioRxiv - Microbiology 2024Quote: ... and plasmids from positive clones were extracted using the NucleoSpin Plasmid Kit (Macherey-Nagel). Throughout the whole cloning process ...
-
bioRxiv - Cell Biology 2024Quote: ... washed in PBS and genomic DNA was extracted by NucleoSpinTM Tissue kit (Macherey-Nagel), according to the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2024Quote: Total RNA was extracted using the NucleoSpin RNA kit (Macherey-Nagel) and reverse-transcribed into cDNA using the ReverTra Ace qPCR RT Master Mix (Toyobo) ...
-
bioRxiv - Microbiology 2024Quote: ... To recover the DNA a Nucleo Spin Gel and PCR Clean up kit (Macherey-Nagel) was used and the DNA was eluted in 20 µl TE buffer (10 mM Tris ...
-
bioRxiv - Plant Biology 2024Quote: A Nucleoshell RP 18 plus reversed-phase column (2 x 100 mm, 2.7 µm; Macherey-Nagel) was used for chromatographic separation with mobile phases (A ...
-
bioRxiv - Plant Biology 2024Quote: ... Chromatographic separation was performed on a Nucleodur C18 HTec column (150 × 2 mm, 1.8 μm particle size; Macherey-Nagel, Duren, Germany) maintained at 30°C ...
-
bioRxiv - Physiology 2024Quote: ... total RNA was extracted from liver and muscle using Nucleospin RNA kit (Macherey-Nagel, 740955.250), then reverse transcription was performed on 1 μg of RNA using SensiFAST cDNA synthesis Kit (Meridian Biosciences ...
-
bioRxiv - Synthetic Biology 2024Quote: ... All plasmid DNA was prepared using the NucleoBond Xtra Midi kit (Macherey-Nagel) and eluted in nuclease-free water.
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... RNA was cleaned up using the NucleoSpin RNA kit (Macherey-Nagel), following the standard protocol ...
-
bioRxiv - Physiology 2024Quote: ... and NMT-treated nonmyr-peptide were spotted (2 µL/spot) on TLC sheets (SIL/UV254, Macherey-Nagel) and separated for about 30 min in a mixture of n-butanol ...
-
bioRxiv - Physiology 2024Quote: Total RNA was isolated using NucleoSpin RNA Plus kit (Macherey-Nagel) following the manufacturer’s instructions ...
-
bioRxiv - Plant Biology 2024Quote: ... DNA was extracted from 3 to 4 leaves of 45 days old plants with a genomic DNA extraction kit (Macherey-Nagel, England). The DNAs of these leaves were combined to sequence the methylome of each plant ...
-
bioRxiv - Synthetic Biology 2024Quote: ... extracted lipids were separated using silica gel-coated thin-layer chromatography (TLC) (0.25 mm Silica gel, DCFertigplatten, Macherey-Nagel, Germany) in a solvent mixture consisting of hexane ...
-
bioRxiv - Plant Biology 2024Quote: ... The separation of substrates was performed at 35°C with a Nucleoshell RP18 column (50 × 3mm, particle size 2.7 μm; Macherey-Nagel, Germany) using an Agilent 1290 High-Pressure Liquid Chromatography (HPLC ...
-
bioRxiv - Plant Biology 2024Quote: ... Twenty seedlings were pooled per replicate and total RNA was extracted from homogenised plant tissue using a phenol-based RNA isolation method (NucleoZol, Macherey-Nagel, Catalogue No.: 740404). PolyA+ libraries were prepared by and sequenced at BGI Genomics (Hong Kong ...
-
bioRxiv - Physiology 2024Quote: RNA isolation was performed using the NucleoSpin®RNA Kit (Macherey-Nagel, Germany) according to the manufacturers’ instructions ...
-
bioRxiv - Microbiology 2024Quote: ... The DNA was extracted from the pellet using the instructions provided by a commercial kit (Macherey-Nagel GmbH & Co.). Each reaction consisted of 500 nM of primer FWD and REV (Table S3 ...