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Citations for Macherey-Nagel GmbH :
501 - 550 of 3263 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: Total RNA was extracted using Nucleospin RNA kit (Macherey-Nagel, 740955) or RNeasy Plus Micro (Qiagen ...
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bioRxiv - Cell Biology 2024Quote: ... and total RNA was collected using a NucleoSpin RNA kit (Macherey-Nagel, #740955). Two technical replicates for each condition were pooled together to obtain an experimental replicate ...
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bioRxiv - Microbiology 2024Quote: ... PCR-amplified DNA was cleaned with NucleoSpin Gel and PCR Clean-up kit (MACHEREY-NAGEL, Düren, Germany). Plasmid DNA was purified using QIAprep Spin Miniprep Kit (QIAGEN ...
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bioRxiv - Microbiology 2024Quote: ... the targeted one was purified from a gel using the NucleoSpin® Gel and PCR Clean-up (MACHEREY-NAGEL, Düren, Germany) or the Monarch® DNA Gel Extraction Kit (New England Biolabs) ...
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bioRxiv - Microbiology 2024Quote: One hundred μl of the bacteria cultured in TSB as described above were pipetted onto a filter paper (Ø 25 mm, Macherey-Nagel, Germany) and placed in the glass odour adapters with the help of sterile tweezers ...
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bioRxiv - Evolutionary Biology 2024Quote: ... we used the NucleoSpin Blood QuickPure Kit (Macherey-Nagel). Extract quality was first assessed on a 1.5% agarose gel to exclude extractions with insufficient yield or excessively degraded DNA ...
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bioRxiv - Plant Biology 2024Quote: ... RNA was extracted using the NucleoSpin RNA Plus kit (Macherey-Nagel) and cDNA was synthesized with the RevertAid First Strand cDNA Synthesis Kit (Thermo Scientific) ...
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bioRxiv - Plant Biology 2024Quote: ... and the resulting PCR reactions were cleaned-up using the NucleoSpin Gel and PCR Clean-up XS kit (Macherey-Nagel). All amplicons obtained from a single biological replicate were pooled in equimolar amounts and a total of 100 ng were used for library preparation following the modified Illumina Nextera Flex library preparation protocol described before (28 ...
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bioRxiv - Genomics 2024Quote: Total RNA was extracted from 100 mg of rosette leaves from ddm1-2 plants using the Nucleo-spin RNA Plant mini kit (Macherey-Nagel). Library preparation and Nanopore sequencing were performed as previously described (Domínguez et al ...
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bioRxiv - Genomics 2024Quote: ... The amplified fragment was purified using Nucleospin Gel and PCR-Clean-Up (Macherey-Nagel, 740609.50) and 1.8x HighPrep PCR Clean-up System (Magbio ...
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bioRxiv - Genomics 2024Quote: ... The amplified fragment was purified using Nucleospin Gel and PCR-Clean-Up (Macherey-Nagel, 740609.50) and 1.2x HighPrep PCR Clean-up System (Magbio ...
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bioRxiv - Molecular Biology 2024Quote: ... DNA was extracted from the filters using Nucleospin eDNA water kit (Macherey-Nagel) following the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2024Quote: ... DNA fragments were purified with NucleoSpin™ gel and PCR clean-up kits (Macherey-Nagel, Germany). Synthetic promoters were ordered from IDT (Integrated DNA Technologies) ...
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Olive mill wastewater as a source of by-products promoting plant defense against microbial pathogensbioRxiv - Plant Biology 2024Quote: ... Total RNA was extracted using RNA isolation NucleoZol (Macherey-Nagel) according to the manufacturer’s instructions and treated with RQ1 RNase-free DNase (Promega) ...
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bioRxiv - Plant Biology 2024Quote: ... Total RNA was extracted using NucleoSpin RNA Plant Kits and DNase I treatment according to the manufacturer’s instructions (Macherey-Nagel, Germany). Complementary DNA (cDNA ...
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bioRxiv - Molecular Biology 2023Quote: DNA extraction from dried filters was done using NucleoSpin™ Forensic Filter kit (Macherey-Nagel GmbH & Co .KG, Düren – Germany). Filter parts were folded and incubated in 600 μL of lysis buffer T1 at 56 °C for two hours with tube horizontally agitated and then centrifugated 1 min 30 sec at 11,000 g ...
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bioRxiv - Molecular Biology 2023Quote: ... using the NucleoSpin Blood Kit (Macherey-Nagel; Dueren, DE) following the manufacturer’s protocol and used as template in diagnostic PCR ...
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bioRxiv - Molecular Biology 2023Quote: ... Genomic DNA was extracted using a NucleoSpin Blood kit (Macherey-Nagel) following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... and purified using the “PCR Clean-up System” (Macherey-Nagel).
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bioRxiv - Molecular Biology 2023Quote: The NucleoSpin® Tissue Kit (Macherey-Nagel, Düren, Germany) combined with the use of zirconia beads (BioSpec ...
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bioRxiv - Neuroscience 2023Quote: Total RNA was isolated from mouse tissues using the Nucleospin RNA Plus kit (Macherey-Nagel, cat# 740984.50), following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: Genomic DNA was extracted from only the 13C-acetate grown cultures that yielded enough DNA at 94 days of incubation using the NucleoSpin Microbial DNA kit (Macherey-Nagel) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... the cellular RNA was extracted by NucleoSpin RNA Kit (Macherey-Nagel GmbH&Co. KG, Germany) for real-time PCR analysis of SARS-CoV-2 E genes using the iTaq Universal Probes One-Step RT-PCR Kit (Bio-Rad ...
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bioRxiv - Microbiology 2023Quote: ... Low copy plasmids were extracted by NucleoBond Xtra Midi kit (MACHEREY-NAGEL) with the other plasmids were extracted by plasmids midi kit (QIAGEN ...
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bioRxiv - Plant Biology 2023Quote: PCR products were pooled by 6 to 12 according to the linkers and cleaned using the Nucleospin Gel and PCR clean up (Macherey-Nagel). Samples containing asymptomatic plants were pooled separately from those containing symptomatic leaves to limit potential cross-contaminations of highly concentrated viruses in symptomatic pools ...
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bioRxiv - Plant Biology 2023Quote: ... RNA was isolated was using the NucleoSpin® RNA Plant Kit (Macherey-Nagel, Düren, Germany) according to the manufacturer’s guidelines ...
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bioRxiv - Plant Biology 2023Quote: ... approximately 50 mg of ground tissue were extracted using the NucleoSpin RNA Plant Kit (Macherey-Nagel, Düren, Germany) following the user guidelines ...
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bioRxiv - Plant Biology 2023Quote: Total RNA was extracted from the roots of 21-days old mock- and SA190-colonized Arabidopsis grown on ½ MS with or without 25% PEG with the Nucleospin RNA plant kit (Macherey-Nagel) following the manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2023Quote: ... purified using the NucleoSpin plasmid kit (Macherey-Nagel, Düren, Germany), were confirmed by sequencing.
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bioRxiv - Microbiology 2023Quote: DNA was extracted for Termignon cheese isolates and all new strains sequenced using the Illumina technology for this project from fresh haploid mycelium and conidia after monospore isolation and growth for five days on malt agar using the Nucleospin Soil Kit (Macherey-Nagel). Sequencing was performed using the Illumina paired-end technology (Illumina Inc. ...
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bioRxiv - Microbiology 2023Quote: ... and NucleoBond® Xtra Midi Plus (MACHEREY-NAGEL, Düren, Germany) for small and large scales ...
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bioRxiv - Microbiology 2023Quote: ... Replicon RNA was extracted using NucleoSpin®RNA (MACHEREY-NAGEL) at the indicated time points and its copy number was measured using the THUNDERBIRD Probe One-step qRT-PCR Kit (TOYOBO CO. ...
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bioRxiv - Microbiology 2023Quote: Genomic DNA was extracted following standard protocols using the NucleoSpin Microbial DNA Mini kit (Macherey-Nagel, Düren, Germany). DNA was quantified using a Qubit 3 fluorometer in combination with dsDNA BR or HS assay kits (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... PCR products were purified using the Nucleospin® extract II kit (Macherey-Nagel) according to manufacturer’s instructions and were Sanger sequenced (Eurofins UK).
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bioRxiv - Microbiology 2023Quote: ... PCR-column purified (Macherey-Nagel), and sent for Sanger sequencing at Genewiz with the primers in Table 2.
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bioRxiv - Microbiology 2023Quote: ... 50 μl of a 40% slurry Ni-NTA agarose (20 μl beads, Macherey-Nagel) were added followed by an incubation for 45 min at 4°C with shaking ...
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bioRxiv - Molecular Biology 2023Quote: ... and rDNase (Macherey-Nagel) and reverse-transcribed with ReverTra Ace® (TOYOBO ...
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bioRxiv - Molecular Biology 2023Quote: ... The ligated products were transformed into XL1 blue bacterial cells by electroporation followed by isolating the plasmids from single bacterial colonies using NucleoBond Xtra Midi kit (Macherey-Nagel). The Cas9-sgRNA plasmids were validated by BbsI restriction analysis and confirmed by Sanger sequencing ...
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bioRxiv - Molecular Biology 2023Quote: ... kit with NTB binding buffer (#740595.150, Macherey-Nagel) according to the manufacturer’s instructions and eluted in 30 µL.
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bioRxiv - Pathology 2023Quote: ... we isolated mtDNA from postnuclear fractions with the alkaline denaturation-neutralization procedure (Nucleospin Plasmid Mini kit, Macherey-Nagel). Liver tissue was homogenized and nuclei pelleted (1000g 10 min ...
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bioRxiv - Plant Biology 2023Quote: ... Total DNA was extracted using CTAB (Doyle & Doyle 1987) and purified using NucleoSpin® Purification Kit (Macherey-Nagel, Düren, Germany). DNA extractions were profiled in Agarose gel 1% and using Nanodrop ...
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bioRxiv - Zoology 2023Quote: ... Germany) or on a hydrophilic interaction liquid chromatography (HILIC) Nucleodur column (125 × 3 mm, 3 μm, Macherey-Nagel, Düren, Germany) for normalphase chromatography ...
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bioRxiv - Plant Biology 2023Quote: Total RNA was extracted from Arabidopsis seedling tissues with NucleoZOL Reagent (Macherey-Nagel) according to the manufacturer’s protocol and gene expression analysis was carried out as previously described (Locci et al. ...
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bioRxiv - Microbiology 2023Quote: RNA was isolated using the NucleoSpin RNA kit from Macherey-Nagel according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... floral organs and fruits using the “NucleoSpin RNA Plant” kit (Macherey-Nagel). After DNase I treatment ...
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bioRxiv - Biochemistry 2023Quote: Propagated plasmids were purified using NucleoSpin® Plasmid from Macherey-Nagel, MN (Germany ...
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bioRxiv - Cell Biology 2023Quote: ... RNAs were extracted using NucleoSpin RNA kit (Macherey-Nagel, Düren, Germany). Then ...
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A randomized multiplex CRISPRi-Seq approach for the identification of critical combinations of genesbioRxiv - Genetics 2023Quote: ... All colonies were scraped off plates using an inoculation loop and added directly to the A1 buffer of a Nucleospin Plasmid Purification Kit (Macherey-Nagel 740499.250) for plasmid isolation and elution in water ...
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bioRxiv - Microbiology 2023Quote: ... RNA was isolated and purified from cells using the NucleoSpin RNA extraction kit (Macherey-Nagel). Following RNA isolation ...
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bioRxiv - Microbiology 2023Quote: Amplicons were purified with the NucleoSpin kit (MACHEREY-NAGEL), sheared in a Bioruptor Pico (Diagenode ...