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Citations for Macherey-Nagel GmbH :
101 - 150 of 3263 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... RNA samples were purified with the rDNase set and the RNA Clean-up kit (Macherey-Nagel, Düren, Germany). RNA quality was checked with the Prokaryote Total RNA Nano Series II chip using the Agilent Bioanalyzer 2100 (Agilent Technologies ...
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bioRxiv - Molecular Biology 2024Quote: Total RNA form cell lysates was obtained using a NucleoSpin® RNA kit (Macherey-Nagel), according to the manufacturer’s instructions and with on-column DNAse treatment ...
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bioRxiv - Molecular Biology 2024Quote: ... Conidia were harvested by filtering through a fluted filter (Nucleo Bond folded filters, Macherey-Nagel, Dueren, Germany), washed in sterile tap water and their concentration was determined with a hemocytometer ...
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bioRxiv - Microbiology 2024Quote: ... and the resultant PCR product gel extracted using a NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel). flaB promoter-lacI and pQE30 were Q5 PCR-amplified from pJSB252 using primers PA468 + PA471 (for pind template ...
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bioRxiv - Genomics 2024Quote: ... The PCR products were purified using NucleoSpin Gel and PCR Clean-Up kits (Macherey-Nagel) and subsequently sent for Sanger sequencing (ElimBio ...
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bioRxiv - Genomics 2024Quote: RNA was extracted using either the Qiagen RNeasy kit or the Macherey Nagel RNAplus kit (Macherey-Nagel, Düren, Germany). RNA-seq libraries were prepared using the TruSeq Stranded mRNA Sample Prep (Illumina ...
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bioRxiv - Molecular Biology 2024Quote: ... 4°C and the supernatant was added to Ni-NTA columns and His-tagged proteins were purified according to the Protino Ni-TED protein purification kit (Macherey-Nagel, Düren, Germany).
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bioRxiv - Molecular Biology 2024Quote: ... DNA in the supernatant was precipitated with isopropanol and suspended in 250 μL of STE (10 mM Tris-HCl [pH 8.0], 1 mM EDTA [pH 8.0], 100 mM NaCl containing 80 μg/mL RNaseA [Macherey-Nagel]). RNA was digested by incubation for 1 hr at 37°C ...
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bioRxiv - Molecular Biology 2024Quote: ... 7.5% β-mercaptoethanol and 10 μg/mL RNaseA (Macherey-Nagel)) and incubated for 1 hr at 37°C ...
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bioRxiv - Molecular Biology 2024Quote: ... All plasmids were maintained in Escherichia coli and purified with a standard plasmid midi-prep kit (NucleoBond® Xtra Midi, MACHEREY-NAGEL, DE).
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bioRxiv - Molecular Biology 2024Quote: ... Genomic DNA was purified according to the manufacturer’s instructions (NucleoBond HMW DNA, Macherey-Nagel). A total of 280ug of genomic DNAs was used for PCR amplification of sgRNA sequences ...
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bioRxiv - Molecular Biology 2024Quote: 1 µg of total RNA was prepared by column purification using the NucleoSpin® RNA Kit (Macherey-Nagel; #740955.250) and transcribed into cDNA using 0.5 µl RevertAid Reverse Transcriptase (Fisher Scientific #EP0441) ...
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bioRxiv - Molecular Biology 2024Quote: ... extracted by using the NucleoSpin® Plasmid or NucleoBond® Xtra Midi Kit (Macherey-Nagel; #740588.250 and #740410.50) and controlled by Sanger Sequencing (Eurofins Genomics or Seqlab Microsynth).
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bioRxiv - Molecular Biology 2024Quote: ... All PCR or vector digestion products were purified using the NucleoSpin® Gel and PCR Clean-Up Kit (Macherey-Nagel; #740609.50). The final plasmids were transformed into competent E.coli XL1-Blue ...
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bioRxiv - Molecular Biology 2024Quote: ... Samples were resuspended in two volumes of buffer NTB (Macherey-Nagel; #740595.150) and DNA was purified using the NucleoSpin® Gel and PCR Clean-Up Kit (Macherey-Nagel ...
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bioRxiv - Molecular Biology 2024Quote: ... and Agilent microarray analyses were performed from isolated total RNA using the NucleoSpin® RNA Kit (Macherey-Nagel; #740955.250). Per reaction 200 ng RNA was amplified and Cy3-labeled using the LIRAK kit (Agilent ...
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bioRxiv - Molecular Biology 2024Quote: ... and DNA was purified using the NucleoSpin® Gel and PCR Clean-Up Kit (Macherey-Nagel; #740609.50) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... Peptides were desalted and concentrated using Chromabond C18WP spin columns (Macherey-Nagel; #730522). Finally ...
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bioRxiv - Molecular Biology 2024Quote: ... Chromatography of 10 µL of each sample was performed using a Nucleoshell C18 reversed phase column (Macherey-Nagel, Düren, Germany) for human bile acids ...
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bioRxiv - Microbiology 2024Quote: ... PCR products were purified with the NucleoSpin Gel and PCR Clean-up kit (MACHEREY-NAGEL, Düren, Germany). Plasmids were purified with the QIAprep Spin Miniprep Kit (QIAGEN ...
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bioRxiv - Microbiology 2024Quote: RNA extraction from cultured Z310 cells was performed using the Nucleospin RNA kit (Macherey-Nagel) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: Endotoxin-free plasmids were purified using silica columns (NucleoBond Xtra Midi EF, Macherey-Nagel/Takara Bio, US). Plasmids expressing red fluorescent protein mRuby341 at 25–100 ng/µL under CAG promoter in combination with ASAP328 voltage sensor at 30–100 ng/µL under either CAG ...
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bioRxiv - Neuroscience 2024Quote: ... followed by filtration through a Chromafil filter from MACHEREY-NAGEL.
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bioRxiv - Neuroscience 2024Quote: ... 5µm column from MACHEREY-NAGEL (Düren, Germany) was used for purification ...
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bioRxiv - Molecular Biology 2024Quote: ... and purified with the NucleoBondXtra Maxi Plus kit (#740416, Macherey-Nagel).
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bioRxiv - Neuroscience 2024Quote: ... Maxi-preps of the plasmid construct were isolated using a Nucleobond Xtra Maxi Plus EF kit (Macherey-Nagel). The full coding frame of all pRK5-rPanx1 and pRK5-mPanx1 constructs were sequenced at the University of Calgary Sequencing Core using the primers CMV-F (5′-CGCAAATGGGCGGTAGGCGTG-3′ ...
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bioRxiv - Microbiology 2024Quote: Following spin column purification (Macherey-Nagel), the PCR products were directly labeled with Alexa Fluor 555 dye and purified using the FISH Tag DNA Orange Kit (ThermoFisher Scientific) ...
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bioRxiv - Neuroscience 2024Quote: ... and purified using an endotoxin-free midiprep kit (NucleoBond Xtra Midi EF, Macherey-Nagel). Purified plasmid DNA was diluted to a final concentration of 135-150 ng/µL in a solution containing (in mM) ...
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bioRxiv - Genetics 2024Quote: RNA was extracted from a total of 24 samples of male brain (four vgll3*EE and four vgll3*LL individuals for each of the three time points) using a NucleoSpin RNA kit (Macherey-Nagel GmbH & Co. KG). The collected samples were transferred to tubes containing 1.4 mm ceramic beads from Omni International ...
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bioRxiv - Evolutionary Biology 2024Quote: DNA was extracted from mycelium and conidia with the Nucleospin soil kit for the progeny and with the NucleoBond High Molecular Weight DNA kit (Macherey-Nagel, Düren, Germany) for the parental strain LCP06136 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Genomic DNA was extracted from fresh mycelium and conidia suspension after single-ascospore isolation and growth for five days on MEA media using the Nucleospin soil kit (Macherey-Nagel, Düren, Germany). For checking that isolated spores were recombinant offspring and not asexual conidia ...
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bioRxiv - Microbiology 2024Quote: ... RNA was extracted using the NucleoSpin® RNA Plant Kit (MACHEREY-NAGEL, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... RNA extraction utilized the NucleoSpin® RNA Midi kit (MACHEREY-NAGEL, US), and cDNA synthesis was carried out using the iScriptTM cDNA synthesis kit (Bio-Rad ...
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bioRxiv - Microbiology 2024Quote: ... The linear fragments were purified on agarose gels (NucleoSpin Gel and PCR Clean-up, Macherey-Nagel). Following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... purified (NucleoSpin Plasmid, Macherey-Nagel) and verified by DNA sequencing ...
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bioRxiv - Molecular Biology 2024Quote: Total RNA was isolated from primary mouse hepatocytes using NucleoSpin kit (Macherey-Nagel cat# 740955.25) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... the NucleoSpin Plasmid Mini kit (MACHEREY-NAGEL) was used for minipreparations ...
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bioRxiv - Molecular Biology 2024Quote: ... Plasmid DNA containing the ACE-tRNA TTT sequences was midiprepped with the NucleoBond PC 20 miniprep kit (Macherey-Nagel). 16HBE14o-cells were diluted to 6 x 105 cells per mL with 1 mL added per well of a coated 6-well plate as previously described22 ...
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bioRxiv - Molecular Biology 2024Quote: ... The pUC57 mini plasmid used as carrier cDNA for transfections was gigaprepped as per kit instructions (Macherey-Nagel). The normalized ACE-tRNA library cDNA at 50 ng/μL was mixed at equal volumes with pUC57 mini carrier plasmid at 150 ng/μL to give final concentrations of 25 ng/μL and 75 ng/μL of the library and carrier plasmids respectively.
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bioRxiv - Molecular Biology 2024Quote: ... 96-well kit was used to miniprep the plasmid DNA (Macherey-Nagel). All plasmid DNA was normalized to 50 ng/μL and the sequence verified by Sanger sequencing (Eurofins Scientific) ...
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bioRxiv - Immunology 2024Quote: HIV RNA was extracted from plasma samples collected from either HIV-1 infected or uninfected humanized hNSG mice transplanted with CD4+ T cells from PWH in the absence or presence of immunotherapy using NucleospinTM RNA Virus kit (Cultek, Macherey-Nagel) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... gDNA from T cells and frozen liver biopsies was extracted with NucleoSpin® Blood kit (Macherey-Nagel) and TCRβ sequencing was performed as previously described45.
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bioRxiv - Evolutionary Biology 2024Quote: ... DNA extraction was performed with the NucleoBond High Molecular Weight DNA kit from Macherey-Nagel, with the mechanical disruption of about 30 mg of lyophilized mycelium with two beads of 3 mm for 5 min at 30 Hz ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The DNA extractions were performed using the NucleoSpin Plant II from MACHEREY-NAGEL kit ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... and extracted RNA using the Nucleospin miRNA kit (Macherey-Nagel; Duren, Germany), which extracts both large RNA (>200 bp ...
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bioRxiv - Neuroscience 2024Quote: ... Total RNA was isolated using the Nucleospin RNA (Macherey-Nagel, Germany) and quantified for purity in a Nanodrop® (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... Genomic DNA was extracted with the NucleoSpin Tissue DNA extraction kit (Macherey-Nagel). PCR products were cleaned with AMPure PB beads using the manufacturer’s instructions at a ratio of 0.6X ...
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bioRxiv - Neuroscience 2024Quote: ... Genomic DNA was extracted with the NucleoSpin Tissue DNA extraction kit (Macherey-Nagel). PCRs were run using between 10-500 pg of DNA measured by Quant-iT Qubit dsDNA HS Assay Kit (ThermoFisher ...
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bioRxiv - Microbiology 2024Quote: ... aureus strains was performed using Nucleospin Microbial DNA kit (Macherey-Nagel, Germany). In order to examine the mutation caused by the CRISPR/Cas system in the target gene regions ...
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bioRxiv - Microbiology 2024Quote: ... peptide samples were desalted using C18 solid phase extraction spin columns (Macherey-Nagel). Peptide mixtures were then analysed using LC-MS on an Exploris 480 instrument connected to an Ultimate 3000 RSLCnano and a nanospray flex ion source (all Thermo Scientific) ...