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Citations for Macherey-Nagel GmbH :
51 - 100 of 3300 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... RNAlater was removed then tissues were transferred into screw-capped tube pre-filled with 350 µl of RA1 buffer of NucleoSPin RNAXS kit (Macherey-Nagel Cat# SKU: 740902.250). Tissues were homogenised with 10 pcs of 3 mm Acid-Washed Zirconium Beads (OPS diagnostics Cat# BAWZ 3000-300-23 ...
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bioRxiv - Microbiology 2024Quote: ... Total RNA for quantitative reverse transcription PCR (qRT-PCR) was isolated using the NucleoSpin® RNA Kit (Macherey-Nagel), including DNA digestion ...
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bioRxiv - Cell Biology 2024Quote: ... RNA was isolated using the NucleoSpin RNA (Macherey-Nagel #740955) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: Total RNA was extracted using the NucleoSpin RNA kit (Macherey-Nagel) and reverse-transcribed into cDNA using the ReverTra Ace qPCR RT Master Mix (Toyobo) ...
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bioRxiv - Microbiology 2024Quote: ... and plasmids from positive clones were extracted using the NucleoSpin Plasmid Kit (Macherey-Nagel). Throughout the whole cloning process ...
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bioRxiv - Microbiology 2024Quote: ... PO4 concentrations were analysed after filtering extraction solution (Mn 619 G1/4, Macherey-Nagel) using continuous flow analyser (Seal Analytical ...
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bioRxiv - Microbiology 2024Quote: ... PCR products for assembly were purified using the NucleoSpin Gel and PCR Clean- up Kit (Macherey-Nagel), and plasmids from positive clones were extracted using the NucleoSpin Plasmid Kit (Macherey-Nagel) ...
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bioRxiv - Microbiology 2024Quote: ... To recover the DNA a Nucleo Spin Gel and PCR Clean up kit (Macherey-Nagel) was used and the DNA was eluted in 20 µl TE buffer (10 mM Tris ...
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bioRxiv - Cell Biology 2024Quote: ... washed in PBS and genomic DNA was extracted by NucleoSpinTM Tissue kit (Macherey-Nagel), according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... 7.5% β-mercaptoethanol and 10 μg/mL RNaseA (Macherey-Nagel)) and incubated for 1 h at 37°C ...
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bioRxiv - Neuroscience 2024Quote: ... genomic DNA was isolated using a Macherey-Nagel Nucleospin Blood kit (Macherey-Nagel; Cat. No. 740951.50) or Macherey-Nagel Nucleospin Blood L kit (Macherey-Nagel ...
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bioRxiv - Cancer Biology 2024Quote: ... PCR products were purified using NucleoSpin Gel and PCR Clean-up Kit (MACHEREY-NAGEL, 740.609.250) and subsequently subjected to Sanger sequencing through Microsynth Seqlab services ...
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bioRxiv - Molecular Biology 2024Quote: ... Freezing and RNA isolation was done following the instructions of the NucleoSpin RNA/Protein Kit (Macherey-Nagel; Düren, Germany). 1 µg of the extracted total RNA per sample was reverse transcribed using oligo(dt)18 primers (First Strand cDNA Synthesis Kit ...
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bioRxiv - Cancer Biology 2024Quote: ... PCR reaction was performed on the new synthetized vector to amplify the HA1-DCK*-P2A-GFP-HA2 sequence corresponding to the HDR (homology directed repair) DNA template and was purified using the NucleoSpin PCR Clean-up kit (Macherey-Nagel # 740609.10) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... RNA was isolated from whole plant tissue ground in liquid nitrogen using the Nucleospin RNA Plant Kit (Macherey-Nagel, Düren, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... Lipid classes were separated using an Agilent 1200 HPLC system using a 150 mm×3 mm (length × internal diameter) 5 µm diol column (Macherey-Nagel), at 40°C ...
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bioRxiv - Plant Biology 2024Quote: ... Total RNA was extracted using Nucleospin® RNA (Macherey-Nagel) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: Total RNA was isolated using an NucleoSpin® RNA Plant Mini kit (Macherey-Nagel) according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2024Quote: Fecal pellets (∼40 mg) were weighed in sterile ceramic bead tubes (NucleoSpin Bead Tubes; Macherey-Nagel, Dueren, Germany) and extracted with 1 mL of chilled methanol (−20 °C ...
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bioRxiv - Zoology 2024Quote: ... RNA from 150 µl of excreta solution was extracted by adding 600 µl of RAV1 lysis buffer and using NucleoSpin virus RNA kit (Macherey-Nagel, France).
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bioRxiv - Zoology 2024Quote: ... total RNA was extracted from the second pooled sample of 60 bees using a NucleoSpin®RNA kit (Macherey-Nagel Gmbh & Co.), cDNA was synthesized from 800 ng total RNA using the iScript cDNA synthesis kit (Bio-Rad Laboratories) ...
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bioRxiv - Zoology 2024Quote: ... Genomic (g)DNA was extracted and purified using the NucleoSpin®Tissue kit following the manufacturer’s instructions (Macherey-Nagel Gmbh & Co. KG, Düren, Germany). Primers used for the detection of all pathogens are found in Supplementary Data 3.
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bioRxiv - Physiology 2024Quote: ... mRNA from CD11B+ cells was extracted using an RNA XS Plus extraction kit (Macherey-Nagel®).
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bioRxiv - Plant Biology 2024Quote: ... PCR products were purified using the NucleoSpin Gel and PCR Cleanl1Jup kit (MACHEREY-NAGEL) and introduced into linearized (with KpnI ...
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bioRxiv - Plant Biology 2024Quote: Leaf total RNA was extracted using the NucleoZOL protocol (Macherey-Nagel) followed by RNA purification using Agencourt RNA Clean XP beads (Beckman-Coulter) ...
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bioRxiv - Biochemistry 2024Quote: ... pcDNA5/FRT-SUGCT and pOG44 plasmid DNA was purified by NucleoBond Xtra Midi Plus EF DNA purification Kit (Macherey-Nagel). Purified pOG44 and pcDNA5/FRT-SUGCT plasmids were co-transfected into Flp-In 293 cells at a 9:1 ratio by using Lipofectamine 2000 reagent (Invitrogen ...
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bioRxiv - Molecular Biology 2024Quote: ... A 200 ul aliquot was used for RNA extraction using a NucleoMag Virus kit (Macherey-Nagel, Düren, Germany) and a Magnetic Particle Processor (MPP ...
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bioRxiv - Microbiology 2024Quote: ... The resulting PCR product was purified using the Nucleospin Gel and PCR clean-up kit (Macherey-Nagel Cat#740609.50), and resolved on a 8% polyacrylamide TBE gel (Life Technologies Cat#EC62162BOX) ...
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bioRxiv - Microbiology 2024Quote: ... 0.5 g of soil were used for DNA extraction using the NucleoSpin Soil Kit (Macherey-Nagel, Düren, Germany) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... All positive samples were purified with the NucleoSpin Gel and PCR Clean-up kit by Macherey-Nagel (ref: 740609.50S). Briefly ...
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bioRxiv - Microbiology 2024Quote: ... Each mix was then purified using the NucleoSpin Gel and PCR Clean-up kit by Macherey-Nagel (ref: 740609.50S). DNA was then eluted and quantified at a Nanodrop 2000 Spectrophotometer (ThermoScientific) ...
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bioRxiv - Microbiology 2024Quote: ... was initially used followed by extraction using the NucleoSpin® RNA kit (Macherey-Nagel). Isolated RNA was then subjected to DNase treatment using the TURBO DNA-free Kit (Life Technologies ...
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bioRxiv - Microbiology 2024Quote: ... Amplicons were purified with the NucleoSpin gel and PCR Clean-up (Macherey-Nagel).
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bioRxiv - Microbiology 2024Quote: ... nitrite (NO2-) and nitrate (NO3-) were filtered (0.2 µm polyethersulfone; MACHEREY-NAGEL, Germany) and stored on ice ...
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bioRxiv - Microbiology 2024Quote: ... DNA was extracted following the standard NucleoSpin Tissue Kit (Macherey-Nagel 740952) protocol for animal tissue with 1 hour of incubation for the first lysis step ...
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bioRxiv - Cell Biology 2024Quote: ... NucleoSpin Gel and PCR Clean-up Kit (Macherey-Nagel) was used according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... cDNA purification was performed using the Nucleospin gel and PCR clean-up kit (Macherey-Nagel, 740609.50) following manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... The three constructs were then cultured for 24 h at 37°C and purified with NucleoBond Xtra Midi Kit (Macherey-Nagel, Germany) and used for gene reporter assay.
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bioRxiv - Molecular Biology 2024Quote: Total RNA was isolated using NucleoSpin RNA kit for RNA purification (Macherey-Nagel; 740955). Reverse transcription was carried out with 1 μg of total RNA using random hexamer primers and the SuperScript IV reverse transcriptase kit (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2024Quote: ... The constructs were purified by NucleoBond® Xtra Midi EF (MACHEREY-NAGEL). Myc-GluA2 was kindly provided by Dr ...
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bioRxiv - Microbiology 2024Quote: ... RNA samples were purified with the rDNase set and the RNA Clean-up kit (Macherey-Nagel, Düren, Germany). RNA quality was checked with the Prokaryote Total RNA Nano Series II chip using the Agilent Bioanalyzer 2100 (Agilent Technologies ...
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bioRxiv - Molecular Biology 2024Quote: ... Conidia were harvested by filtering through a fluted filter (Nucleo Bond folded filters, Macherey-Nagel, Dueren, Germany), washed in sterile tap water and their concentration was determined with a hemocytometer ...
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bioRxiv - Microbiology 2024Quote: ... and the resultant PCR product gel extracted using a NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel). flaB promoter-lacI and pQE30 were Q5 PCR-amplified from pJSB252 using primers PA468 + PA471 (for pind template ...
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bioRxiv - Genomics 2024Quote: RNA was extracted using either the Qiagen RNeasy kit or the Macherey Nagel RNAplus kit (Macherey-Nagel, Düren, Germany). RNA-seq libraries were prepared using the TruSeq Stranded mRNA Sample Prep (Illumina ...
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bioRxiv - Molecular Biology 2024Quote: ... 4°C and the supernatant was added to Ni-NTA columns and His-tagged proteins were purified according to the Protino Ni-TED protein purification kit (Macherey-Nagel, Düren, Germany).
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bioRxiv - Molecular Biology 2024Quote: ... DNA in the supernatant was precipitated with isopropanol and suspended in 250 μL of STE (10 mM Tris-HCl [pH 8.0], 1 mM EDTA [pH 8.0], 100 mM NaCl containing 80 μg/mL RNaseA [Macherey-Nagel]). RNA was digested by incubation for 1 hr at 37°C ...
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bioRxiv - Molecular Biology 2024Quote: ... 7.5% β-mercaptoethanol and 10 μg/mL RNaseA (Macherey-Nagel)) and incubated for 1 hr at 37°C ...
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bioRxiv - Molecular Biology 2024Quote: ... All plasmids were maintained in Escherichia coli and purified with a standard plasmid midi-prep kit (NucleoBond® Xtra Midi, MACHEREY-NAGEL, DE).
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bioRxiv - Molecular Biology 2024Quote: 1 µg of total RNA was prepared by column purification using the NucleoSpin® RNA Kit (Macherey-Nagel; #740955.250) and transcribed into cDNA using 0.5 µl RevertAid Reverse Transcriptase (Fisher Scientific #EP0441) ...
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bioRxiv - Molecular Biology 2024Quote: ... extracted by using the NucleoSpin® Plasmid or NucleoBond® Xtra Midi Kit (Macherey-Nagel; #740588.250 and #740410.50) and controlled by Sanger Sequencing (Eurofins Genomics or Seqlab Microsynth).