Citations for Macherey-Nagel GmbH :
1 - 50 of 2030
citations
since 2017
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... PCR products were purified using the Nucleospin Gel and PCR Clean-Up kit (Macherey-Nagel). Single-stranded RNA of positive and negative polarity was synthesized by in vitro transcription and purified as described above ...
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bioRxiv - Cell Biology 2022Quote: ... and purified using the Nucleospin Gel and PCR Clean-Up kit (Macherey-Nagel). In vitro transcription reaction was carried out in a final volume of 100 μl transcription mix containing 80 mM HEPES (pH 7.5) ...
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bioRxiv - Cell Biology 2022Quote: ... PCR products were purified from agarose gels using the Nucleospin Gel and PCR Clean-Up kit (Macherey-Nagel). EIF2AK2 and EIF2S1 sequences were inserted into pET-His 1a vector using the Gibson Assembly Cloning Kit (New England Biolabs ...
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bioRxiv - Plant Biology 2022Quote: Total RNA was extracted from 50mg of root using the NucleoSpin® RNA Kit (Macherey-Nagel). cDNAs were synthesized with the M-MLV Reverse Transcriptase (Promega ...
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bioRxiv - Genetics 2022Quote: ... DNA was then extracted from tissue using the NucleoSpin Tissue Mini kit columns and corresponding protocol for extraction from animal tissue (Macherey-Nagel, Duren, Germany). For each sperm pool ...
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bioRxiv - Cell Biology 2022Quote: ... Plasmid purification was performed using NucleoBond Xtra Midi EF Kit from Macherey-Nagel. Inserts were sequenced at Eurofins MWG Operon ...
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bioRxiv - Neuroscience 2022Quote: ... Mature worms were frozen as whole at − 20°C and DNA was later extracted using NucleoSpin Tissue Mini kit for DNA from cells and tissue (Macherey-Nagel). PCR was performed with OneTaq Quick-Load 2x Master Mix with Standard Buffer (New England Biolabs) ...
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bioRxiv - Neuroscience 2022Quote: RNA was extracted from purified astrocyte preparations using RNA-binding columns (NucleoSpin RNA plus Macherey-Nagel #740984.250). RNA was analyzed using TapeStation ...
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bioRxiv - Neuroscience 2022Quote: RNA from purified astrocytes or brain cell suspension after enzymatic digestion was extracted using RNA-binding columns (NucleoSpin RNA plus Macherey-Nagel #740984.250), genomic DNA was eliminated and cDNA was synthetised (Thermo Fisher Scientific #K1672) ...
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bioRxiv - Neuroscience 2022Quote: ... purified (Macherey-Nagel #740416.10), and packaged into AAV8 vector (packaging was done by University of Helsinki AAV core unit) ...
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bioRxiv - Cell Biology 2022Quote: Total RNA extracts were obtained using the Nucleospin RNA kit (Macherey-Nagel) and cDNAs were synthetized using SuperScript III reverse transcriptase (Thermofisher Scientific) ...
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bioRxiv - Microbiology 2022Quote: ... The samples (40 μl) were injected onto a MN Nucleosil C18 analytical column (250 mm x 4 mm i.d., 100-5) (Macherey-Nagel, Duren, Germany), using a flow rate of 0.8 mL.min−1 at 27 °C ...
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bioRxiv - Cell Biology 2022Quote: ... total RNA extracts were obtained using the Nucleospin RNA kit (Macherey-Nagel) and cDNAs were synthesized using SuperScript III reverse transcriptase (Thermofisher Scientific) ...
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bioRxiv - Cell Biology 2022Quote: ... The HB-pbDDIKO and HB-pbDDIKD plasmids were purified using the NucleoBond® Xtra Midi plasmid DNA purification kit (MACHEREY-NAGEL), linearized with NotI-KasI ...
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bioRxiv - Cell Biology 2022Quote: ... purified using the NucleoBond® Midi plasmid DNA purification kit (MACHEREY-NAGEL), and used for transfection of P ...
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bioRxiv - Bioengineering 2022Quote: ... RNA extraction was carried out using the nucleospin RNA kit (Macherey-Nagel). The RNAs were quantified using a Nanodrop and the retro-transcription made using the high-capacity cDNA reverse transcription kit (Thermo Fisher) ...
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bioRxiv - Microbiology 2022Quote: ... extracellular viral RNA was extracted using the NucleoSpin RNA virus kit (Macherey-Nagel) and intracellular viral RNA using the E.Z.N.A Total RNA Kit I (Omega Bio-tek) ...
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bioRxiv - Microbiology 2022Quote: ... The viral RNA in each sample was extracted using the NucleoSpin RNA virus kit (Macherey-Nagel) and quantified by qRT-PCR.
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bioRxiv - Molecular Biology 2022Quote: ... Plasmids were extracted from colonies selected on LB agar medium containing ampicillin (100 μg mL-1) using the NucleoSpin Plasmid extraction kit (Macherey-Nagel) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... Reverse cross-linked chromatin was purified using the NucleoSpin™ Gel and PCR Clean-up kit (Macherey-Nagel) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... Plasmids were isolated using an endotoxin-free plasmid midiprep kit (Macherey-Nagel) for QF2 lines and NucleoBond Xtra Midi Endotoxin-Free plasmid kit (Clontech 740420.50 ...
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bioRxiv - Cell Biology 2022Quote: ... RNA was isolated from confluent cells from one well of a 6-well plate with the NucleoSpin RNA isolation kit (Macherey-Nagel) according to manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: DNA from electroporated HSC was extracted using the tissue XS kit (Macherey-Nagel) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... The resulting PCR product was ‘cleaned up’ using a NucleoSpin Gel and PCR clean-up kit (Macherey-Nagel). Capped and Poly(A ...
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bioRxiv - Biochemistry 2022Quote: ... Coli using NuceloBond Xtra Maxi Kit per the manufacturers recommendations (Macherey-Nagel). All scFv-ACE2 ...
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bioRxiv - Cancer Biology 2022Quote: Total DNA extraction was performed using NucleoSpin® Tissue mini kit (Macherey-Nagel, Germany). RNA was extracted using NucleoSpin® RNA mini kit (Macherey-Nagel ...
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bioRxiv - Cancer Biology 2022Quote: ... RNA was isolated usin Nucleospin RNA (Macherey-Nagel) according to the manufacturers protocol ...
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bioRxiv - Biochemistry 2022Quote: ... Plasmid DNA was isolated using the NucleoSpin Plasmid kit (Macherey-Nagel) and analysed by Sanger sequencing (Eurofins Genomics).
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bioRxiv - Biochemistry 2022Quote: ... The PCR products were isolated using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel). Standard molecular cloning techniques were further employed to insert the genes of interest into target vectors using restriction nucleases (New England Biolabs) ...
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bioRxiv - Biochemistry 2022Quote: ... The final library was purified using NucleoSpin columns (Macherey-Nagel) and sequenced on a NextSeq 500 Illumina sequencer.
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bioRxiv - Developmental Biology 2022Quote: ... In vitro transcribed mRNAs were purified using Macherey-Nagel Nucleospin® RNA Clean-up kit (Macherey-Nagel, Bethlehem, PA) prior to injections.
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bioRxiv - Developmental Biology 2022Quote: ... Total RNA was extracted by using the Macherey-Nagel Nucleospin® RNA Clean-up XS kit (Macherey-Nagel, Bethlehem, PA). cDNA was synthesized using iScript cDNA synthesis kit (Bio-Rad ...
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bioRxiv - Bioengineering 2022Quote: ... All generated constructs were digested (20 U Dpn1; 2 h, 37 °C) and purified using PCR clean-up kit (Macherey-Nagel). As eGFP-control ...
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bioRxiv - Bioengineering 2022Quote: ... The remaining cuticular wax-chloroform mixture was filtered (cellulose folded filter paper; 4-12 µm pore size, M&N 615 1/4, Ø 185 mm, Macherey-Nagel GmbH & Co. KG), and chloroform was evaporated (rotary evaporator ...
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bioRxiv - Bioengineering 2022Quote: ... Plasmid extraction and polymerase chain reaction (PCR) purification kits were ordered from Macherey-Nagel GmbH & Co ...
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bioRxiv - Bioengineering 2022Quote: ... purified using PCR clean-up kit (Macherey-Nagel), and transformed in electrocompetent E ...
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bioRxiv - Bioengineering 2022Quote: ... Derivatization was done using 20 µl N,O-bis(trimethylsilyl)-trifluoroacetamid (BSTFA; Macherey-Nagel, Düren, Germany) and 20 µl pyridine (Sigma Aldrich Corp. ...
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bioRxiv - Bioengineering 2022Quote: Total RNA was isolated using NucleoSpin RNA II Kit (Macherey-Nagel) in pursuance of the manufacturer’s protocol ...
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bioRxiv - Biophysics 2022Quote: ... TEV-protease and unprocessed hsTANGO1 were removed by Protino-Kit (Macherey-Nagel). Flowthrough and wash fractions were collected ...
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bioRxiv - Cell Biology 2022Quote: ... Total RNA was isolated from wt and Uhrf1- or Usp7-knockdown BHK cells using the nucleospin triprep kit from Macherey-Nagel. 500 ng of total RNA was reverse transcribed with a high-capacity cDNA reverse transcription kit (Applied Biosystems ...
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bioRxiv - Cell Biology 2022Quote: ... lysates were centrifuged at 21000 g for 10 min and supernatants were transferred to silica membrane spin columns (Macherey-Nagel) and centrifuged at 11000 g for 1 min ...
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bioRxiv - Cell Biology 2022Quote: Total RNA was isolated using NucleoSpin mini RNA kit (Macherey-Nagel, Düren, Germany) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: RNA was isolated from cells using a NucleoSpin RNA Plus kit (Macherey-Nagel) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2022Quote: Genomic DNA was isolated with Macherey-Nagel− NucleoSpin− Blood kit (740951.50, Macherey-Nagel) according to manufacturer’s instruction ...
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bioRxiv - Genomics 2022Quote: ... the DNA was purified using NucleoSpin columns (Macherey-Nagel) and sequenced on a NextSeq 500 Illumina sequencer.
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bioRxiv - Developmental Biology 2022Quote: ... Transfer was carried out on to NCP Porablot Membrane (Macherey-Nagel; Cat#12807411) for 90mins at 90V at 4°C ...
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bioRxiv - Genomics 2022Quote: RNA was extracted using NucleoSpin RNA extraction kit (Macherey-Nagel) according to the manufacturer instructions ...
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bioRxiv - Evolutionary Biology 2022Quote: ... we removed the last segments of the caudal section and preserved it in 96% ethanol before DNA extraction using the NucleoSpin® 96 Tissue kit (Macherey-Nagel). It is worth noting that the ablation of the last segments of the caudal section of earthworms does not affect their survival due to their regeneration capacity (e.g ...
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bioRxiv - Evolutionary Biology 2022Quote: Genomic DNA was extracted using NucleoSpin plant II kit from Macherey-Nagel following the instructions ...
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bioRxiv - Immunology 2022Quote: RNA from sorted BMDCs was extracted using the Macherey-Nagel™ NucleoSpin™ RNA Plus kit (Macherey-Nagel™) and quantified using the Qubit RNA HS Assay Kit (Thermo Fisher Scientific) ...