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Citations for Macherey-Nagel GmbH :
1 - 50 of 3263 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Diurnal regulation of SOS Pathway and Sodium Excretion Underlying Salinity Tolerance of Vigna marinabioRxiv - Plant Biology 2024Quote: ... we extracted genomic DNA from the unexpanded leaves with Nucleobond HMW DNA kit (MACHEREY-NAGEL GmbH & Co. KG, Düren, Germany), size-selected the DNA with Short Read Eliminatior XL kit (Pacific Biosciences of California ...
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bioRxiv - Plant Biology 2024Quote: ... Genomic DNA was extracted using plant DNA extraction kit (Macherey-Nagel). DNA samples were processed as described in Schalk et al ...
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bioRxiv - Physiology 2024Quote: ... standard incubation control and after 1,2,4 h at hypoxic conditions) was extracted using the NucleSpin RNA Kit (Macherey-Nagel, Düren, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: DNA was extracted from blood samples of patients II-8 and IV-1 using the Nucleospin extraction kit (ref 740952-Macherey-Nagel). Subsequently ...
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bioRxiv - Plant Biology 2024Quote: ... gel-purified with the NucleoSpin Gel and PCR Cleanl1Jup kit (MACHEREY-NAGEL) and assembled using the In-Fusion HD Cloning Kit (Takara Bio) ...
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bioRxiv - Plant Biology 2024Quote: RNA was extracted from 6-days-old seedlings grown in sterile culture using the RNA NucleoSpin Plant kit (Macherey-Nagel) according to the manufacturer’s instructions with on-column DNase treatment ...
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bioRxiv - Plant Biology 2024Quote: ... Total RNA was isolated using NucleoSpin™ columns (Macherey-Nagel), employing a standardized protocol adapted from Sangha et al ...
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bioRxiv - Plant Biology 2024Quote: DNA-free RNA was isolated with NucleoSpin® RNA Plant and Fungi Kit as recommended by the manufacturer (MACHEREY-NAGEL) and used as template for reverse transcription as previously described (Lemaire-Chamley et al. ...
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bioRxiv - Neuroscience 2024Quote: ... the bands of interest were excised and purified with the Gel and PCR clean up (ref 740609, Macherey-Nagel) with a final elution volume of 20 µl ...
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bioRxiv - Neuroscience 2024Quote: ... and DNA extracted with the Nucleospin tissue kit following the manufacturer recommendations (ref 740952, Macherey-Nagel) for large amount of cells (300.000 cells) ...
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bioRxiv - Neuroscience 2024Quote: ... RNA was purified with the Nucleospin RNA isolation kit (catalog no. 740955.250; Macherey-Nagel) with initial quantitation conducted using a NanoDrop ND-1000 spectrophotometer (Thermo Fisher Scientific) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... the plasmid DNA was amplified using a NucleoBond Xtra Midi kit (Cat.#740410; Macherey-Nagel) and the sequence was verified with Sanger sequencing (GENEWIZ).
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bioRxiv - Molecular Biology 2024Quote: ... The TEV eluate was incubated with 200 µl pre-washed Protino® Ni-NTA Agarose beads (Macherey-Nagel) for 1 h at 4°C in a rotating wheel ...
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bioRxiv - Molecular Biology 2024Quote: ... The sample was then acidified using trifluoroacetic acid and peptides were purified using Chromabond C18 Microspin columns (Macherey-Nagel).
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bioRxiv - Microbiology 2024Quote: ... or Nucleospin Plasmid Kit (Macherey-Nagel). DNA-modifying enzymes and restriction enzymes were purchased from New England Biolabs ...
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bioRxiv - Microbiology 2024Quote: ... or the NucleoSpin Gel and PCR Clean-up Kit (Macherey-Nagel). All constructed plasmids were sequenced by Microsynth AG (Balgach ...
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bioRxiv - Neuroscience 2024Quote: ... Peptides were then desalted and concentrated using Chromabond C18WP spin columns (Macherey-Nagel, Part No. 730522). Finally ...
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bioRxiv - Microbiology 2024Quote: ... was loaded onto Protino® Ni-NTA Agarose (Macherey-Nagel, Düren, Germany) in a gravity column ...
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bioRxiv - Systems Biology 2024Quote: ... Octadecyl C18 Empore disks were purchased from 3M and Polygoprep 300-20 C18 powder was purchased from Macherey-Nagel. Centrifugal filters were from Amicon ...
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bioRxiv - Synthetic Biology 2024Quote: ... and purified edited cells using a NucleoSpin Tissue Kit (Macherey-Nagel). The HDRT integration site was PCR-amplified from genomic DNA using Q5 High-Fidelity Polymerase (NEB) ...
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Targeted Perturb-seq Reveals EGR1 and FOS as Key Regulators of the Transcriptional RAF-MAPK ResponsebioRxiv - Systems Biology 2024Quote: ... The PCR product (325 bp) was purified from a 1% agarose gel via NucleoSpin columns (Macherey-Nagel). NGS was performed on an Illumina Miniseq using a MiniSeq Mid Output Kit (300-cycles ...
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bioRxiv - Immunology 2024Quote: ... the PCR product was purified using the PCR Clean-up kit (Macherey-Nagel 740609.250) and quantified by Qubit dsDNA BR (Invitrogen Q33265) ...
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bioRxiv - Microbiology 2024Quote: DNA was extracted using 100μl of the homogenate of crushed ticks according to the Genomic DNA from tissue kit (Macherey-Nagel, Germany) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: Total RNA was extracted from 100μl of the homogenate of crushed ticks using the NucleoSpin RNA kit (Macherey-Nagel, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... The cells were lysed with Nucleobond buffer set iii (MACHEREY-NAGEL) and the chromosomal DNA was purified with Sera-Mag magnetic beads (Cytiva) ...
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bioRxiv - Microbiology 2024Quote: Total RNA from cell lysates was isolated with a NucleoSpin RNA purification kit (Macherey-Nagel). Purified whole cell RNA was converted to cDNA using the high-capacity cDNA reverse transcription kit (Applied Biosystems) ...
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Targeted Perturb-seq Reveals EGR1 and FOS as Key Regulators of the Transcriptional RAF-MAPK ResponsebioRxiv - Systems Biology 2024Quote: ... PCR products were purified using NucleoSpin columns (Macherey-Nagel). The library vector pMB1-10x was prepared by restriction digestion with AarI (Thermo Fisher ...
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Targeted Perturb-seq Reveals EGR1 and FOS as Key Regulators of the Transcriptional RAF-MAPK ResponsebioRxiv - Systems Biology 2024Quote: ... The digestion reaction was run on a 1% agarose gel followed by excision of the digested band and purification via NucleoSpin columns (Macherey-Nagel). 100 ng digested pMB1-10x and 2.4 ng amplified sgRNA library insert were assembled using Gibson Assembly Master Mix (NEB ...
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bioRxiv - Systems Biology 2024Quote: ... Total worm RNA was extracted by using a column-based kit from Macherey-Nagel. RNA-seq was performed by BGI with the BGISEQ-500 platform ...
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bioRxiv - Synthetic Biology 2024Quote: ... Spheroplasts were collected by centrifugation and RNA was isolated using the NucleoSpin RNA Plus kit (Macherey-Nagel). RNA quality and integrity was determined by Qubit fluorometry using an RNA BR Assay Kit (ThermoFisher) ...
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bioRxiv - Evolutionary Biology 2024Quote: DNA extraction was performed using a combination protocol between the NucleoSpin® Plant II kit from Macherey-Nagel and the DNeasy plant mini kit from QIAGEN ...
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bioRxiv - Plant Biology 2024Quote: ... and subjected to genomic DNA extraction using the Nucleo Mag Plant kit (Macherey-Nagel GmbH &Co. KG, Germany) in the automated nucleic acid extractor Maelstrom 4800 (TANBead Taiwan Advanced Nanotech Inc. ...
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bioRxiv - Plant Biology 2024Quote: ... the PCR products were purified using a NucleoSpin® Gel and PCR Clean-up kit (Macherey-Nagel). Sequencing reactions were made up of 20-25/ng of the purified PCR product ...
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bioRxiv - Physiology 2024Quote: Lung tissue total RNA was extracted in NucleoZOL (740404.200; Macherey-Nagel, German). The quality and integrity of extracted RNA were evaluated using Nanodrop Specthophotometer ...
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bioRxiv - Plant Biology 2024Quote: ... RNA was extracted using the NucleoSpin® RNA Plant kit (Macherey-Nagel) according to the manufacturer’s instructions and quantified with a Nanodrop spectrophotometer ...
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bioRxiv - Plant Biology 2024Quote: RNA extraction was performed with NucleoSpin® RNA plus kit (Macherey-Nagel). RNA extraction was performed with leaves from 28-days-old healthy plants (6 plants ...
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bioRxiv - Plant Biology 2024Quote: ... The samples were pulverized in liquid nitrogen and RNA was extracted using the NucleoZOL reagent (MACHEREY-NAGEL, Germany), following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... Samples (80 µL) were separated on a NUCLEOSIL® C18 column (EC 250/4, 300-5, MACHEREY-NAGEL GmbH, Germany) in a LaChrome Elite HPLC equipped with an L-2130 pump module ...
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bioRxiv - Plant Biology 2024Quote: ... The soluble protein was first purified by affinity chromatography with Protino nickel nitrilotriacetic acid agarose (Macherey-Nagel) using the lysis buffer but with the imidazole concentration changed to sequential rounds of 20 mM and 250 mM for washing and eluting the protein ...
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bioRxiv - Plant Biology 2024Quote: ... The supernatants were transferred to fresh tubes and filtrated first through a 5 µm syringe PES filter (CHROMAFIL® Xtra PES, 5.0 µm, Macherey-Nagel), then through a 1 µm (Whatman™ Puradisc ...
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bioRxiv - Plant Biology 2024Quote: RNA was isolated by NucleoSpin RNA Plant Kit (Macherey-Nagel 1806/001) according to manufacturer’s instruction ...
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bioRxiv - Plant Biology 2024Quote: ... Total RNA was isolated using the NucleoSpin RNA Plus Mini kit (Macherey-Nagel, Hoerdt, France) following manufacturer’s recommendations.
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bioRxiv - Plant Biology 2024Quote: ... Lipid classes were separated using an Agilent 1200 HPLC system using a 150 mm×3 mm (length × internal diameter) 5 µm diol column (Macherey-Nagel, Germany), at 40°C ...
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bioRxiv - Plant Biology 2024Quote: ... After solid-phase extraction on HR-XC (Chromabond, Macherey-Nagel, www.mn-net.com), 10 µl of the eluate were analyzed via UPLC–MS/MS ...
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bioRxiv - Plant Biology 2024Quote: ... The protein was purified using Protino Ni-NTA agarose (Macherey-Nagel, #7450400-500).
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bioRxiv - Neuroscience 2024Quote: ... or Macherey-Nagel Nucleospin Blood L kit (Macherey-Nagel; Cat. No. 740954.20), depending on the number of cells recovered from FACS ...
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bioRxiv - Genetics 2024Quote: ... Genomic DNA was isolated using NucleoBond HMW DNA (MACHEREY-NAGEL) according to the manufacturer’s protocol.
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bioRxiv - Bioengineering 2024Quote: ... PCR products from genomic DNA and cDNA were purified using NucleoSpin Gel and PCR Clean-up Mini Kit (Macherey-Nagel, Allentown, PA, USA), and quality was validated via agarose gel electrophoresis.
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bioRxiv - Plant Biology 2024Quote: ... total RNA was extracted from approximately 100 mg of ground tissue using the NucleoSpin RNA Plant Kit (Macherey-Nagel) and treated with DNase I on the column ...
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bioRxiv - Plant Biology 2024Quote: ... mRNA was extracted using the NucleoSpin RNA Plant Kit (Macherey-Nagel 740949) modified to increase first RA3 buffer wash to 650 µl and an additional 400 µl RA3 buffer wash ...