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Citations for Macherey-Nagel GmbH :
1 - 50 of 3058 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... The cells were lysed with Nucleobond buffer set iii (MACHEREY-NAGEL) and the chromosomal DNA was purified with Sera-Mag magnetic beads (Cytiva) ...
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bioRxiv - Microbiology 2024Quote: Total RNA from cell lysates was isolated with a NucleoSpin RNA purification kit (Macherey-Nagel). Purified whole cell RNA was converted to cDNA using the high-capacity cDNA reverse transcription kit (Applied Biosystems) ...
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bioRxiv - Plant Biology 2024Quote: ... Total RNA was extracted using Nucleospin® RNA (Macherey-Nagel) according to the manufacturer’s instructions ...
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bioRxiv - Zoology 2024Quote: ... RNA from 150 µl of excreta solution was extracted by adding 600 µl of RAV1 lysis buffer and using NucleoSpin virus RNA kit (Macherey-Nagel, France).
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bioRxiv - Zoology 2024Quote: ... total RNA was extracted from the second pooled sample of 60 bees using a NucleoSpin®RNA kit (Macherey-Nagel Gmbh & Co.), cDNA was synthesized from 800 ng total RNA using the iScript cDNA synthesis kit (Bio-Rad Laboratories) ...
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bioRxiv - Zoology 2024Quote: ... Genomic (g)DNA was extracted and purified using the NucleoSpin®Tissue kit following the manufacturer’s instructions (Macherey-Nagel Gmbh & Co. KG, Düren, Germany). Primers used for the detection of all pathogens are found in Supplementary Data 3.
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bioRxiv - Systems Biology 2024Quote: Fecal pellets (∼40 mg) were weighed in sterile ceramic bead tubes (NucleoSpin Bead Tubes; Macherey-Nagel, Dueren, Germany) and extracted with 1 mL of chilled methanol (−20 °C ...
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bioRxiv - Plant Biology 2024Quote: Total RNA was isolated using an NucleoSpin® RNA Plant Mini kit (Macherey-Nagel) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: HIV RNA was extracted from plasma samples collected from either HIV-1 infected or uninfected humanized hNSG mice transplanted with CD4+ T cells from PWH in the absence or presence of immunotherapy using NucleospinTM RNA Virus kit (Cultek, Macherey-Nagel) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... gDNA from T cells and frozen liver biopsies was extracted with NucleoSpin® Blood kit (Macherey-Nagel) and TCRβ sequencing was performed as previously described45.
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bioRxiv - Evolutionary Biology 2024Quote: DNA extraction was performed using a combination protocol between the NucleoSpin® Plant II kit from Macherey-Nagel and the DNeasy plant mini kit from QIAGEN ...
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bioRxiv - Cell Biology 2024Quote: ... RNAlater was removed then tissues were transferred into screw-capped tube pre-filled with 350 µl of RA1 buffer of NucleoSPin RNAXS kit (Macherey-Nagel Cat# SKU: 740902.250). Tissues were homogenised with 10 pcs of 3 mm Acid-Washed Zirconium Beads (OPS diagnostics Cat# BAWZ 3000-300-23 ...
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bioRxiv - Cancer Biology 2024Quote: ... PCR products were purified using NucleoSpin Gel and PCR Clean-up Kit (MACHEREY-NAGEL, 740.609.250) and subsequently subjected to Sanger sequencing through Microsynth Seqlab services ...
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bioRxiv - Molecular Biology 2024Quote: ... Freezing and RNA isolation was done following the instructions of the NucleoSpin RNA/Protein Kit (Macherey-Nagel; Düren, Germany). 1 µg of the extracted total RNA per sample was reverse transcribed using oligo(dt)18 primers (First Strand cDNA Synthesis Kit ...
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bioRxiv - Molecular Biology 2024Quote: ... RNA was isolated from whole plant tissue ground in liquid nitrogen using the Nucleospin RNA Plant Kit (Macherey-Nagel, Düren, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... Lipid classes were separated using an Agilent 1200 HPLC system using a 150 mm×3 mm (length × internal diameter) 5 µm diol column (Macherey-Nagel), at 40°C ...
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bioRxiv - Cancer Biology 2024Quote: ... PCR reaction was performed on the new synthetized vector to amplify the HA1-DCK*-P2A-GFP-HA2 sequence corresponding to the HDR (homology directed repair) DNA template and was purified using the NucleoSpin PCR Clean-up kit (Macherey-Nagel # 740609.10) according to the manufacturer’s protocol ...
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Targeted Perturb-seq Reveals EGR1 and FOS as Key Regulators of the Transcriptional RAF-MAPK ResponsebioRxiv - Systems Biology 2024Quote: ... PCR products were purified using NucleoSpin columns (Macherey-Nagel). The library vector pMB1-10x was prepared by restriction digestion with AarI (Thermo Fisher ...
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Targeted Perturb-seq Reveals EGR1 and FOS as Key Regulators of the Transcriptional RAF-MAPK ResponsebioRxiv - Systems Biology 2024Quote: ... The digestion reaction was run on a 1% agarose gel followed by excision of the digested band and purification via NucleoSpin columns (Macherey-Nagel). 100 ng digested pMB1-10x and 2.4 ng amplified sgRNA library insert were assembled using Gibson Assembly Master Mix (NEB ...
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bioRxiv - Systems Biology 2024Quote: ... Total worm RNA was extracted by using a column-based kit from Macherey-Nagel. RNA-seq was performed by BGI with the BGISEQ-500 platform ...
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bioRxiv - Plant Biology 2024Quote: ... The samples were pulverized in liquid nitrogen and RNA was extracted using the NucleoZOL reagent (MACHEREY-NAGEL, Germany), following the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2024Quote: ... Spheroplasts were collected by centrifugation and RNA was isolated using the NucleoSpin RNA Plus kit (Macherey-Nagel). RNA quality and integrity was determined by Qubit fluorometry using an RNA BR Assay Kit (ThermoFisher) ...
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bioRxiv - Molecular Biology 2024Quote: ... 7.5% β-mercaptoethanol and 10 μg/mL RNaseA (Macherey-Nagel)) and incubated for 1 h at 37°C ...
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bioRxiv - Neuroscience 2024Quote: ... Peptides were then desalted and concentrated using Chromabond C18WP spin columns (Macherey-Nagel, Part No. 730522). Finally ...
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bioRxiv - Neuroscience 2024Quote: ... genomic DNA was isolated using a Macherey-Nagel Nucleospin Blood kit (Macherey-Nagel; Cat. No. 740951.50) or Macherey-Nagel Nucleospin Blood L kit (Macherey-Nagel ...
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bioRxiv - Genetics 2024Quote: BAC DNA was extracted from BAC clones (BacPac Resources, CHORI, Oakland) using the Nucleobond Xtra BAC kit (Macherey-Nagel) and subsequently labeled (Nick translation protocol ...
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bioRxiv - Plant Biology 2024Quote: ... PCR products were purified using the NucleoSpin Gel and PCR Cleanl1Jup kit (MACHEREY-NAGEL) and introduced into linearized (with KpnI ...
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bioRxiv - Plant Biology 2024Quote: Leaf total RNA was extracted using the NucleoZOL protocol (Macherey-Nagel) followed by RNA purification using Agencourt RNA Clean XP beads (Beckman-Coulter) ...
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bioRxiv - Biochemistry 2024Quote: ... pcDNA5/FRT-SUGCT and pOG44 plasmid DNA was purified by NucleoBond Xtra Midi Plus EF DNA purification Kit (Macherey-Nagel). Purified pOG44 and pcDNA5/FRT-SUGCT plasmids were co-transfected into Flp-In 293 cells at a 9:1 ratio by using Lipofectamine 2000 reagent (Invitrogen ...
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bioRxiv - Biochemistry 2024Quote: ... SSC was separated on a 250 x 3 mm 5 µm Nucleodur HTec C18 (Macherey-Nagel) column ...
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bioRxiv - Cell Biology 2024Quote: ... Total RNA was extracted from the freezed samples using NucleoSpin RNA kit (Macherey-Nagel) and concentration of extracted RNA was measured with Nanodrop spectrophotometer at 260 nm ...
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bioRxiv - Bioengineering 2024Quote: ... The constructs were assembled into the AAV2-ITR plasmid via molecular cloning and purified using the NucleoBond Xtra Maxi EF kit (Macherey-Nagel, Düren, Germany). The purified plasmids were sent to Vigene Biosciences (now Charles River ...
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bioRxiv - Bioengineering 2024Quote: ... PCR products from genomic DNA and cDNA were purified using NucleoSpin Gel and PCR Clean-up Mini Kit (Macherey-Nagel, Allentown, PA, USA), and quality was validated via agarose gel electrophoresis.
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bioRxiv - Plant Biology 2024Quote: ... Samples (80 µL) were separated on a NUCLEOSIL® C18 column (EC 250/4, 300-5, MACHEREY-NAGEL GmbH, Germany) in a LaChrome Elite HPLC equipped with an L-2130 pump module ...
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bioRxiv - Plant Biology 2024Quote: ... total RNA was extracted from approximately 100 mg of ground tissue using the NucleoSpin RNA Plant Kit (Macherey-Nagel) and treated with DNase I on the column ...
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bioRxiv - Plant Biology 2024Quote: ... mRNA was extracted using the NucleoSpin RNA Plant Kit (Macherey-Nagel 740949) modified to increase first RA3 buffer wash to 650 µl and an additional 400 µl RA3 buffer wash ...
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bioRxiv - Plant Biology 2024Quote: Samples were ground using glass beads (2.5 mm) and RNA was extracted using NucleoSpin RNA PLUS extraction kits (Macherey-Nagel) following the manufacturer’s protocols ...
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bioRxiv - Plant Biology 2024Quote: ... a NucleoBond high-molecular-weight DNA kit (MACHEREY-NAGEL, Germany) was used ...
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bioRxiv - Plant Biology 2024Quote: ... and subjected to genomic DNA extraction using the Nucleo Mag Plant kit (Macherey-Nagel GmbH &Co. KG, Germany) in the automated nucleic acid extractor Maelstrom 4800 (TANBead Taiwan Advanced Nanotech Inc. ...
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bioRxiv - Plant Biology 2024Quote: ... the PCR products were purified using a NucleoSpin® Gel and PCR Clean-up kit (Macherey-Nagel). Sequencing reactions were made up of 20-25/ng of the purified PCR product ...
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bioRxiv - Physiology 2024Quote: Lung tissue total RNA was extracted in NucleoZOL (740404.200; Macherey-Nagel, German). The quality and integrity of extracted RNA were evaluated using Nanodrop Specthophotometer ...
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bioRxiv - Plant Biology 2024Quote: ... RNA was extracted using the NucleoSpin® RNA Plant kit (Macherey-Nagel) according to the manufacturer’s instructions and quantified with a Nanodrop spectrophotometer ...
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bioRxiv - Plant Biology 2024Quote: RNA extraction was performed with NucleoSpin® RNA plus kit (Macherey-Nagel). RNA extraction was performed with leaves from 28-days-old healthy plants (6 plants ...
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bioRxiv - Plant Biology 2024Quote: ... The soluble protein was first purified by affinity chromatography with Protino nickel nitrilotriacetic acid agarose (Macherey-Nagel) using the lysis buffer but with the imidazole concentration changed to sequential rounds of 20 mM and 250 mM for washing and eluting the protein ...
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bioRxiv - Plant Biology 2024Quote: ... The supernatants were transferred to fresh tubes and filtrated first through a 5 µm syringe PES filter (CHROMAFIL® Xtra PES, 5.0 µm, Macherey-Nagel), then through a 1 µm (Whatman™ Puradisc ...
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bioRxiv - Plant Biology 2024Quote: RNA was isolated by NucleoSpin RNA Plant Kit (Macherey-Nagel 1806/001) according to manufacturer’s instruction ...
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bioRxiv - Plant Biology 2024Quote: ... Total RNA was isolated using the NucleoSpin RNA Plus Mini kit (Macherey-Nagel, Hoerdt, France) following manufacturer’s recommendations.
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bioRxiv - Plant Biology 2024Quote: ... Lipid classes were separated using an Agilent 1200 HPLC system using a 150 mm×3 mm (length × internal diameter) 5 µm diol column (Macherey-Nagel, Germany), at 40°C ...
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bioRxiv - Plant Biology 2024Quote: ... After solid-phase extraction on HR-XC (Chromabond, Macherey-Nagel, www.mn-net.com), 10 µl of the eluate were analyzed via UPLC–MS/MS ...
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bioRxiv - Physiology 2024Quote: ... mRNA from CD11B+ cells was extracted using an RNA XS Plus extraction kit (Macherey-Nagel®).