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Citations for Macherey-Nagel GmbH :
1 - 50 of 3263 citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... by using the NucleoSpin RNA virus kit (Macherey-Nagel). For intracellular viral RNA cells were harvested in TRI Reagent and RNA extraction (Zymo Research ...
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bioRxiv - Microbiology 2024Quote: ... PCR clean-up (Macherey-Nagel Cat. No. 740609.250S), and Sanger sequencing (Microsynth GmbH ...
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bioRxiv - Cancer Biology 2024Quote: ... and genomic DNA was extracted using the Nucleospin Blood L Midi genomic DNA extraction kit (Macherey-Nagel, 740954.20). Genomic DNA was PCR amplified by the Genetic Perturbation Platform at the Broad Institute using the following primers:
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bioRxiv - Cancer Biology 2024Quote: ... RNA was isolated using the NucleoSpin RNA Mini kit (Macherey-Nagel, #740955.250). DNase digestion was performed on a column as recommended by the manufacturer ...
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bioRxiv - Cancer Biology 2024Quote: RNA was isolated from cells with the NucleoSpin RNA Plus Kit (Macherey-Nagel, 740984) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... and clean-up using the NucleoSpin RNA Clean-up Mini Kit (Macherey-Nagel, #740948.50). RNA quality was surveyed using RNA ScreenTapes (Agilent ...
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bioRxiv - Cancer Biology 2024Quote: Cell lysis and extraction of genomic DNA from dry pellets were performed using the NucleoSpin Tissue XS kit (Macherey-Nagel). Genomic DNA quality and quantity were measured using the NanoDrop (ND-1000 ...
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bioRxiv - Cancer Biology 2024Quote: Genomic DNA was isolated with the NucleoSpin Tissue kit (MACHEREY-NAGEL) and hybridized to Illumina MethylationEPIC BeadChips (at Life and Brain ...
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bioRxiv - Microbiology 2024Quote: ... Amplicons were purified with the NucleoSpin gel and PCR Clean-up (Macherey-Nagel).
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bioRxiv - Molecular Biology 2024Quote: ... Plasmid DNA containing the ACE-tRNA TTT sequences was midiprepped with the NucleoBond PC 20 miniprep kit (Macherey-Nagel). 16HBE14o-cells were diluted to 6 x 105 cells per mL with 1 mL added per well of a coated 6-well plate as previously described22 ...
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bioRxiv - Molecular Biology 2024Quote: ... The pUC57 mini plasmid used as carrier cDNA for transfections was gigaprepped as per kit instructions (Macherey-Nagel). The normalized ACE-tRNA library cDNA at 50 ng/μL was mixed at equal volumes with pUC57 mini carrier plasmid at 150 ng/μL to give final concentrations of 25 ng/μL and 75 ng/μL of the library and carrier plasmids respectively.
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bioRxiv - Molecular Biology 2024Quote: ... 96-well kit was used to miniprep the plasmid DNA (Macherey-Nagel). All plasmid DNA was normalized to 50 ng/μL and the sequence verified by Sanger sequencing (Eurofins Scientific) ...
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bioRxiv - Microbiology 2024Quote: ... DNA was extracted following the standard NucleoSpin Tissue Kit (Macherey-Nagel 740952) protocol for animal tissue with 1 hour of incubation for the first lysis step ...
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bioRxiv - Molecular Biology 2024Quote: Total RNA was isolated using NucleoSpin RNA kit for RNA purification (Macherey-Nagel; 740955). Reverse transcription was carried out with 1 μg of total RNA using random hexamer primers and the SuperScript IV reverse transcriptase kit (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2024Quote: ... we added 6 µL of rDNAse buffer and 0.6 µL rDNAse (Macherey-Nagel) per sample and incubated at 37°C for 10 min ...
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bioRxiv - Microbiology 2024Quote: ... nitrite (NO2-) and nitrate (NO3-) were filtered (0.2 µm polyethersulfone; MACHEREY-NAGEL, Germany) and stored on ice ...
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bioRxiv - Zoology 2024Quote: ... The purified plasmid was prepared using the NucleoBond® Xtra Midi kit (MACHEREY-NAGEL, Düren, Germany) or the Genopure Plasmid Midi kit (Roche Diagnostics GmbH ...
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bioRxiv - Developmental Biology 2024Quote: ... PCR products were run on gel electrophoresis before gel extraction of bands of interest (with sizes corresponding to either a wild-type allele or a deleted allele) (Nucleospin gel and PCR clean-up, Macherey-Nagel Cat# 740609.50). Fragments were cloned into blunt-end TOPO vector (Invitrogen Cat# 45024) ...
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bioRxiv - Cancer Biology 2024Quote: ... The resulting amplicons were purified using the NucleoSpin gel and PCR cleanup kit following the manufacturer’s instructions (Macherey-Nagel Inc., Bethlehem, PA) and submitted for Sanger sequencing with the same primers used to generate the amplicons ...
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bioRxiv - Developmental Biology 2024Quote: ... and resistant clones then used for miniprep plasmid preparations (Nucleospin plasmid, Macherey-Nagel, Cat # 740588.50), followed by sequencing ...
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bioRxiv - Cancer Biology 2024Quote: ... RNA was isolated from Eµ-Myc lymphoma cells using a NucleoSpin RNA kit (Macherey-Nagel) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: Fosmid DNA of the hit clones was extracted with the NucleoBond Xtra Midi kit from Macherey-Nagel (France) following supplier recommendations ...
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bioRxiv - Bioengineering 2024Quote: ... The acquired plasmid was purified using NucleoSpin Plasmid EasyPure (Macherey-Nagel) kit according to suppliers instructions ...
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bioRxiv - Bioengineering 2024Quote: ... were gel purified using NucleoSpin Gel and PCR Clean-up (Macherey-Nagel) kit according to suppliers’ instructions ...
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bioRxiv - Cancer Biology 2024Quote: Genomic DNA were extracted from cells using the NucleoSpin Tissue kit (Macherey-Nagel) and stored in TE buffer ...
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bioRxiv - Cell Biology 2024Quote: ... RNA was purified using the Qiagen RNeasy Mini kit with DNase (Macherey-Nagel, #740955.250) treatment ...
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bioRxiv - Cell Biology 2024Quote: Total RNA of LGTV-infected cells was extracted using NucleoSpin RNA Plus kit (Macherey-Nagel) and eluted in 60 µl RNase-free H2O according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... DNA was extracted using a Nucleospin PCR cleanup kit (Macherey-Nagel) and resuspended in 50 μl elution buffer (5 mM Tris-HCl [pH 8.5]) ...
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bioRxiv - Biochemistry 2024Quote: ... Expressed proteins were captured on pre-packed Ni-IDA (Protino Ni-IDA Resin, Macherey-Nagel, Duren, Germany) columns ...
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bioRxiv - Microbiology 2024Quote: ... Genomic DNA was then extracted following the Nucleospin Tissue Kit protocol (Macherey-Nagel) and resuspended in 50 μl elution buffer (5 mM Tris-HCl [pH 8.5]) ...
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bioRxiv - Microbiology 2024Quote: ... RNA was extracted using the RNA XS kit (Macherey-Nagel, Düren, Germany) from MS4 Ciona juveniles that underwent gut colonization ...
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bioRxiv - Cancer Biology 2024Quote: RNA was isolated using the NucleoSpin® RNA Plus kit following the manufacturer’s protocol (Macherey-Nagel, Düren, Germany). The integrity and concentration of RNA samples was assessed using the TapeStation 2200 (Agilent ...
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bioRxiv - Cancer Biology 2024Quote: Total RNA isolation was performed according to the manufacture protocol with NucleoSpin RNA Plus kit (Macherey-Nagel). RNA was retrotranscribed with Reverse Transcriptase Superscript IV (Invitrogen) ...
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bioRxiv - Cell Biology 2024Quote: Genomic DNA was isolated using the NucleoSpin Tissue Kit (Macherey-Nagel) and quantified on a NanoDrop ND-2000 spectrophotometer (Thermo Scientific) ...
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bioRxiv - Cell Biology 2024Quote: Total RNA was isolated using the NucleoSpin RNA kit (Macherey-Nagel, Düren, Germany), quantified with the NanoDrop ND-1000 spectrophotometer (Thermo Scientific ...
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bioRxiv - Biochemistry 2024Quote: ... The CBP-His6-ZZ-Upf1-HD proteins were first purified on a Protino® Ni-NTA agarose resin (Macherey-Nagel). After successive washing steps using buffers C200 first ...
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bioRxiv - Biochemistry 2024Quote: ... Ni-IDA affinity beads (Macherey-Nagel, Bethlehem, PA, USA) were used to purify TBC-DEG assemblies ...
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bioRxiv - Biochemistry 2024Quote: Products formed in in vitro or whole-cell reactions were quantified via GC-FID using a non-chiral OPTIMA™ 5MS column (Macherey-Nagel GmbH & Co. KG, Düren, Germany). Substrates and products were directly extracted from the aqueous reaction solutions with DCM containing 2 mM acetophenone as an internal standard ...
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bioRxiv - Biochemistry 2024Quote: ... Plasmid preparation kits were purchased from Macherey-Nagel.
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bioRxiv - Biochemistry 2024Quote: ... 4°C and the supernatant was incubated at 4 °C with 1.25 ml Ni-NTA agarose resin (Macherey-Nagel) for 1 h with agitation ...
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bioRxiv - Biochemistry 2024Quote: ... Clarified lysates were filtered (0.45 µm syringe tip filters) and applied to pre-equilibrated Protino Ni-NTA Agarose (Macherey-Nagel) beats in a gravety column ...
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bioRxiv - Cell Biology 2024Quote: ... Total RNA was isolated using the NucleoSpin RNA II kit (Macherey-Nagel), and 1 μg of RNA was reverse-transcribed using M-MLV reverse transcriptase (Promega ...
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bioRxiv - Cell Biology 2024Quote: ... Total RNA was extracted from the freezed samples using NucleoSpin RNA kit (Macherey-Nagel) and concentration of extracted RNA was measured with Nanodrop spectrophotometer at 260 nm ...
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bioRxiv - Cell Biology 2024Quote: ... Total RNA was isolated using NucleoSpin RNA Plus (Macherey-Nagel, Düren, Germany) and reverse transcribed using ReverTra Ace qPCR RT Master Mix (TOYOBO ...
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bioRxiv - Cancer Biology 2024Quote: ... SNU-499 and Hep3B cells was extracted using the NucleoSpin RNA kit (Macherey-Nagel, AH Diagnostics, Solna, Sweden) according to the protocol of the manufacturers ...
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bioRxiv - Cancer Biology 2024Quote: Total RNA was extracted from cells using the kit NucleoSpin RNA (Macherey-Nagel). RNA was then reverse transcribed using material from ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... PCR products were purified using the NucleoSpin Gel and PCR Clean-up kit (Macherey-Nagel) and sequenced using Sanger sequencing (Eton Biosciences ...
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bioRxiv - Cancer Biology 2024Quote: Genomic DNA were isolated from cell lines using the NucleoSpin Tissue extraction kit (Macherey-Nagel). Genomic DNA amount and quality were assessed using a NanoDrop (Thermo Fisher ...
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bioRxiv - Cancer Biology 2024Quote: Total RNA was isolated using the NucleoSpin RNA kit (Macherey-Nagel). For library preparation the TruSeq Stranded mRNA kit (Illumina ...
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bioRxiv - Cell Biology 2024Quote: ... Genomic DNA was purified using Macherey-Nagel NucleoSpin Blood XL Maxi Kit (740950.50, Macherey-Nagel) and prepared as previously described [Kampmann et al 2014] with modifications ...