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Citations for New England Biolabs :
4701 - 4750 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2024Quote: ... and subsequent restriction digest of the plasmid DNA was carried out using the BamHI restriction enzyme (New England BioLabs) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2024Quote: ... were deglycosylated by incubation with PNGase F (New England BioLabs) at 37 °C for one hour ...
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bioRxiv - Biochemistry 2024Quote: ... Twelve cycles of amplification were carried out with Q5 High Fidelity DNA Polymerase (NEB) using an annealing temperature of 68 °C and a 1 min extension at 65 °C ...
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bioRxiv - Biochemistry 2024Quote: ... The plasmids were then assembled via Gibson assembly (New England Biolabs, Cat # E2611L), followed by transformation of E ...
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bioRxiv - Cancer Biology 2024Quote: ... 25μL of the samples and 1μL of the protein ladder (BioLabs) were loaded into a Bio-Red 4-15% Mini-PROTEAN precast polyacrylamide gel ...
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bioRxiv - Biophysics 2024Quote: ... 1.25 U apyrase (NEB), 300 mM TCEP ...
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bioRxiv - Biophysics 2024Quote: ... The corresponding nicking enzymatic reaction using Nb.BsmI and Nb.BsrDI (New England Biolabs, USA) created the complimentary overhangs to match the insert (13) ...
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bioRxiv - Biophysics 2024Quote: ... we developed a two-step protocol using selective strand degradation with lambda exonuclease (New England Biolabs, USA) based on (45) ...
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bioRxiv - Biophysics 2024Quote: ... which is devoid of native cysteines using site-directed mutagenesis (NEB Q5) using primers designed from NEBaseChanger ...
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bioRxiv - Bioengineering 2024Quote: ... and BamHI (New England Biolabs) for 3 h at 37°C ...
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bioRxiv - Bioengineering 2024Quote: Purified magnetic beads were added based on the following reverse transcription mixture (0.2 mM dNTPs, 10 mM DTT, and 0.1 μM RT-Primer [P2_ver2] and Proto Script II RTase™ [New England Biolabs]) and were incubated at 37°C for 40 min.
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bioRxiv - Bioengineering 2024Quote: ... The resulting mixtures were loaded on a 6% native polyacrylamide gel with SDS-free loading dye (NEB). The gel was made with TAE-Mg buffer (0.4 M Tris ...
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bioRxiv - Bioengineering 2024Quote: ... the beads were treated with DNase I (NEB) in the manufacturer’s recommended buffer at 37°C with shaking for 30 minutes ...
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bioRxiv - Bioengineering 2024Quote: A working concentration of T4 nucleotide kinase (PNK, NEB), following the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: Benzylguanine (BG, SNAP tag ligand BG-GLA-NHS, NEB, S9151S) and chloroalkane (CL ...
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bioRxiv - Cancer Biology 2024Quote: ... using the Q5 High-Fidelity DNA polymerase (NEB, M0491), and the following primers/barcodes (table 1):
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bioRxiv - Bioengineering 2024Quote: ... The reaction mixture for polymerization contained 25 units/ml Hot-start Taq DNA polymerase (New England Biolabs), Taq standard reaction buffer ...
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bioRxiv - Cancer Biology 2024Quote: ... ligated vectors were then transformed into NEB10-beta electroporation ultracompetent E.coli cells (NEB® 10-beta Electrocompetent E. coli, NEB, Ref.C3020K) and grown overnight on LB plates supplemented with 50 μg/mL Carbenicillin (Carbenicillin disodium salt ...
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bioRxiv - Cancer Biology 2024Quote: ... ligated vectors were then transformed into NEB10-beta electroporation ultracompetent E.coli cells (NEB® 10-beta Electrocompetent E ...
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bioRxiv - Bioengineering 2024Quote: ... rabbit anti-Sox-2 (New England Biolabs), and rabbit anti-NANOG (New England Biolabs) ...
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bioRxiv - Bioengineering 2024Quote: ... PCR reactions were purified using the Monarch DNA PCR Cleanup Kit (New England Biolabs; T1030). For Gibson assembly of constructs ...
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bioRxiv - Bioengineering 2024Quote: ... coli (New England Biolabs; C3040) made using the Mix&Go transformation kit (Zymo Research ...
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bioRxiv - Bioengineering 2024Quote: ... and rabbit anti-NANOG (New England Biolabs). Briefly ...
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bioRxiv - Bioengineering 2024Quote: Cloning of transcriptional units (TUs) was conducted in accordance with MTK toolkit procedures using NEBridge Golden Gate BsaI Assembly Mastermix (New England Biolabs; E1601) according to the manufacturer’s protocol61 ...
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bioRxiv - Bioengineering 2024Quote: Polymerase chain reaction (PCR) of constructs utilized for plasmid assembly were amplified by NEB Q5 Hot-Start 2X Mastermix according to the manufacturer’s protocol (New England Biolabs ...
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bioRxiv - Bioengineering 2024Quote: ... of constructs utilized for plasmid assembly were amplified by NEB Q5 Hot-Start 2X Mastermix according to the manufacturer’s protocol (New England Biolabs, Ipswitch, MA, USA; M0494L). Amplifications of difficult templates (such as the CAG promoter ...
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bioRxiv - Bioengineering 2024Quote: ... and Ultra II Directional RNA Library Prep Kit (NEB) to isolate mRNA and perform dUTP directional preparation of the mRNA library ...
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bioRxiv - Bioengineering 2024Quote: ... connected to the exposure chamber (NEB-MED H, Braintree Scientific, Inc.). 5 mL of 5mg/mL LPS was used to induce the injury ...
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bioRxiv - Biophysics 2024Quote: ... RNA transcription was performed in vitro with the HiScribe T7 High Yield RNA Synthesis Kit (New England BioLabs). DNA substrates containing the target cleavage site (479 base pairs ...
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bioRxiv - Biophysics 2024Quote: ... We labeled Nup96-SNAP with SNAP-tag ligand O6-benzylguanine conjugated AF647 (BG-AF647, S9136S, New England Biolabs) as described else (26) ...
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bioRxiv - Cancer Biology 2024Quote: ... PCR samples were treated with NEBNext End Repair Module (NEB, E6050) and the adaptor ligated DNA was amplified using NEBNext Q5 Hot Start Hifi PCR Master Mix (NEB ...
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bioRxiv - Cancer Biology 2024Quote: ... and the adaptor ligated DNA was amplified using NEBNext Q5 Hot Start Hifi PCR Master Mix (NEB, M0543). The libraries were sequenced at a rate of 5 million reads per sample ...
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bioRxiv - Biophysics 2024Quote: ... 1.25 U apyrase (NEB), 300 mM TCEP ...
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bioRxiv - Bioengineering 2024Quote: ... genomic sites of interest were amplified with the Q5® High-Fidelity 2X Master Mix (NEB) using conventional thermocycling conditions ...
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bioRxiv - Bioengineering 2024Quote: ... PCR was conducted with the Q5® 2X Master Mix (NEB) and ATP6 Sanger sequencing primers (listed in Supplementary Table S4) ...
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bioRxiv - Bioengineering 2024Quote: ... Ligations were done with the Quick LigationTM Kit (NEB). NEB® Stable Competent E ...
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bioRxiv - Bioengineering 2024Quote: Plasmids encoding TALE-free MTS–G1397-split DddAtox/DddA6/DddA11–UGI were generated with the Q5® Site-Directed Mutagenesis (SDM) Kit (NEB), using the corresponding herein developed backbone plasmids as templates for each final construct containing either the DddAtox ...
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bioRxiv - Bioengineering 2024Quote: ... purified using the Monarch DNA Gel Extraction Kit (NEB), confirmed via Sanger sequencing (Genewiz) ...
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bioRxiv - Bioengineering 2024Quote: ... buffer 4.0 (New England Biolabs) and T5 exonuclease ...
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bioRxiv - Bioengineering 2024Quote: Primers were synthesized by Thermo Scientific and routine PCR amplifications were carried out using Phusion High Fidelity DNA Polymerase (New England Biolabs, UK). Chromosomal modifications were engineered in A ...
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bioRxiv - Bioengineering 2024Quote: S-R1-R2-H stock (87.5 kDa, 2 - 4 μM) tagged with handle oligos was reconstituted in 1x T4 ligase buffer (NEB) in PBS with 0.05% NP-40 ...
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bioRxiv - Bioengineering 2024Quote: ... 1 µL of T4 ligase and 0.5 µL of T4 polymerase (both from NEB) were added to 20 µL of PBS ...
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bioRxiv - Biophysics 2024Quote: ... The plasmid was transformed into BL21(DE3) cells (New England BioLabs) and subsequent restriction digest of the plasmid DNA was carried out using the BamHI restriction enzyme (New England BioLabs ...
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bioRxiv - Cancer Biology 2024Quote: ... PCR fragments were incubated with the T7 Endonuclease I (NEB, M0302S) and nuclease activity was confirmed by DNA agarose electrophoresis.
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bioRxiv - Cancer Biology 2024Quote: ... cDNA was diluted 1:10 and used for qPCR reactions employing Luna® Universal qPCR Master Mix (New England Biolabs). Ct values were computed by the instrument and used for calculation of ΔΔCt using ACTB (Beta-actin ...
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bioRxiv - Bioengineering 2024Quote: ... RNase H (NEB, M0297S) and DNase I (from bovine pancreas ...
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bioRxiv - Bioengineering 2024Quote: ... Primers for SDM (see Supplementary Table S3) were designed using NEBaseChanger (NEB) and synthesized as standard DNA oligos (IDT) ...
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bioRxiv - Bioengineering 2024Quote: ... This strategy avoids 3’-terminal editing of the mismatched primers by the 3’-5’ exonuclease activity of Q5® High-Fidelity DNA Polymerase (NEB), increasing PCR specificity.61
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bioRxiv - Bioengineering 2024Quote: ... PCR was done with the Q5® High-Fidelity 2X Master Mix (NEB). After PCR1 in the nested PCR strategy ...
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bioRxiv - Bioengineering 2024Quote: ... insert and vector bands were separated by agarose gel electrophoresis and purified with the Monarch DNA Gel Extraction Kit obtained from New England Biolabs (NEB). Ligations were done with the Quick LigationTM Kit (NEB) ...