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Citations for New England Biolabs :
4601 - 4650 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... and specific index primers supplied in NEBNext Multiplex Oligo Kit for Illumina (Index Primer Set 1, NEB, E7335L). Conditions for the PCR were as follows ...
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bioRxiv - Cell Biology 2024Quote: ... cleaned up A-tailed DNA went through Adaptor ligation reaction using Quick Ligation Kit (NEB, M2200L) following the manufacturer’s protocol ...
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bioRxiv - Biophysics 2024Quote: ... Cells were left to recover in SOC medium (NEB 10β Stable Outgrowth Medium) for 30 minutes ...
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bioRxiv - Cancer Biology 2024Quote: ... treated with or without RNaseH (New England Biolabs # M0297L). DNA-RNA hybrids were then immunoprecipitated from the digested genomic DNA using S9.6 antibody ...
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bioRxiv - Cancer Biology 2024Quote: ... genomic DNA and plasma-derived cfDNA were digested with the methylation-dependent restriction enzyme LpnPI (New England Biolabs, Ipswich, MA) generating 32 bp DNA fragments containing the methylated CpG in the middle ...
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bioRxiv - Cancer Biology 2024Quote: ... Guide RNAs were generated in house using Highscribe™ T7 Quick High Yield RNA synthesis kit (New England Biolabs Inc.), Alt-R™ S.p ...
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bioRxiv - Cell Biology 2024Quote: ... coli High Efficiency cells (C3019H, New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... Library preparation was performed using the NEBNext Ultra RNA libray Prep Kit for Illumina (NEB, USA). RNA sequencing was performed using the Illumina NovaSeq platform ...
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bioRxiv - Cancer Biology 2024Quote: ... cDNA synthesis was performed using the Luna script RT Supermix kit (New England Biolabs, Cat#M3010L). Quantitative real-time PCR (qRT-PCR ...
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bioRxiv - Cell Biology 2024Quote: ... RNA samples were treated with DNAse I (New England Biosciences) and were concentrated through a Monarch RNA Cleanup Kit (NEB) to ensure no carryover of contaminants.
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bioRxiv - Bioengineering 2024Quote: ... PCR amplification was performed using Q5 High-Fidelity 2X Master Mix (New England Biolabs) for 35 cycles with a 20-second extension time ...
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bioRxiv - Bioengineering 2024Quote: ... in conjunction with NEBNext Multiplex Oligos for Illumina (Unique Dual Index UMI Adaptors RNA Set1, NEB #7416S) and NEBNext Poly(A ...
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bioRxiv - Bioengineering 2024Quote: ... and NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB #E7490L), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... Poly(A) RNA was prepared with the Poly(A) mRNA Magnetic Isolation Module (New England Biolabs, E7490), and the sequencing library was generated using the NEBNext® Ultra™II Directional RNA Library Prep Kit (New England Biolabs ...
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bioRxiv - Biophysics 2024Quote: ... DNA extractions from the agarose slices used β-agarase (NEB) enzymatic digestions as per the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... and Illumina sequencing adaptor ligation (New England BioLabs, E6000B-10) as previously published67 ...
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bioRxiv - Cancer Biology 2024Quote: ... and the sequencing library was generated using the NEBNext® Ultra™II Directional RNA Library Prep Kit (New England Biolabs, E7760). RNA-seq was performed on NovaSeq 6000 (Illumina ...
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bioRxiv - Cancer Biology 2024Quote: Total RNA was extracted with the NEB Monarch RNA miniprep kit (NEB cat. T2010S) following manufacturer’s manual with on-column DNA digestion ...
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bioRxiv - Cancer Biology 2024Quote: ... and SalI-HF (NEB cat. R3138T). The linearization reaction was run on 1% agarose gel ...
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bioRxiv - Biophysics 2024Quote: ... Vector DNA was linearized with NheI or MluI and mRNA synthesized in vitro using the SP6 or T7 AmpliCap Max High Yield Message Maker Kit (Cellscript, USA) or HiScribe® T7 ARCA mRNA Kit (New England Biolabs).
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bioRxiv - Biophysics 2024Quote: ... coli BL21 derivative (T7 Express, NEB) using Luria Broth (Miller ...
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bioRxiv - Biophysics 2024Quote: ... On-column phosphatase treatment was performed by incubating the resin with 10 mL SEC buffer supplemented with 1 mM MnCl2 and 4,000 units (10 μL) Lambda Protein Phosphatase (LPP) (New England BioLabs P0753L) standing at 4°C overnight ...
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bioRxiv - Biophysics 2024Quote: ... The codon optimized full-length LRRK2 sequence was inserted into the same vector used previously via Gibson assembly and LRRK2 mutant as cloned using the Q5 Site-Directed Mutagenesis Kit (NEB) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... C1-deleted pMYs-CIC::DUX4-IRES-EGFP was cloned from pMYs-CIC::DUX4-IRES-EGFP (a gift from Takuro Nakamura (52) and Michael Kyba (25)) using the Q5 Site-Directed Mutagenesis Kit (NEB E0554S) with the primers described in Supplemental Dataset S2 ...
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bioRxiv - Cancer Biology 2024Quote: ... RNA samples were additionally processed with the Monarch RNA Cleanup Kit (NEB), analyzed for integrity with a TapeStation (Agilent) ...
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bioRxiv - Cancer Biology 2024Quote: ... C1- and HMG-domain deletion and point-mutant constructs were cloned from pcDNA3.1-HA-CIC::DUX4 (a gift from Takuro Nakamura (6)) using the Q5 Site-Directed Mutagenesis Kit (NEB E0554S) with the primers described in Supplemental Dataset S2 ...
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bioRxiv - Cell Biology 2024Quote: ... we use the Monarch® Genomic DNA Purification Kit (New England Biolabs) and follow the manufacturer recommendation ...
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bioRxiv - Cell Biology 2024Quote: ... polymerase along with template was used for riboprobe synthesis and RNase free DNase (NEB M0303S) was used for template digestion ...
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bioRxiv - Cancer Biology 2024Quote: ... or NEBaseChanger (New England Biolabs (NEB)) ...
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bioRxiv - Cancer Biology 2024Quote: ... or NEBaseChanger (New England Biolabs (NEB)) ...
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bioRxiv - Cell Biology 2024Quote: ... and phosphatase inhibitors sodium orthovanadate (New England Biolabs, Ipswich, MA, USA; item # P0758) and sodium fluoride (New England Biolabs ...
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bioRxiv - Cell Biology 2024Quote: ... and sodium fluoride (New England Biolabs; item # P0759). Western blotting was performed using a sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE ...
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bioRxiv - Cell Biology 2024Quote: ... and specific index primers supplied in NEBNext Multiplex Oligo Kit for Illumina (Index Primer Set 1, NEB, E7335L). Conditions for the PCR were as follows ...
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bioRxiv - Cell Biology 2024Quote: ... Eluted DNA was enriched by PCR using Phusion High-Fidelity PCR Master Mix kit (NEB, M0531S) and specific index primers supplied in NEBNext Multiplex Oligo Kit for Illumina (Index Primer Set 1 ...
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bioRxiv - Cell Biology 2024Quote: ... and cDNAs were synthesized from 2 µg total RNA with Superscript II reverse transcriptase (New England Biolabs). RT-qPCR was performed in a StepOnePlus™ Real-Time PCR System (Applied BioSystems ...
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bioRxiv - Cell Biology 2024Quote: ... mRNA Magnetic Isolation Module and NEBNext Multiplex Oligos for Illumina (Unique Dual Index UMI Adaptors RNA) following the manufacturer’s instructions (New England Biolabs). Quantification and quality checking of libraries was done using the 4200 Tapestation instrument and a D5000 ScreenTape assay (Agilent Technologies) ...
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bioRxiv - Cell Biology 2024Quote: ... All mGreenLantern-tagged constructs consist of ORFs inserted into the multiple cloning site of pcDNA3.1-mGreenLantern using a Gibson cloning kit (NEBuilder HiFi DNA Assembly Cloning Kit, New England Biolabs). Intersectin(SH35 ...
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bioRxiv - Cancer Biology 2024Quote: Quantitative RT-PCR was done with the Luna Universal qPCR Master Mix (#M3003, NEB,) using a QuantStudio 5 Real-time PCR system (Applied Biosystems) ...
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bioRxiv - Cancer Biology 2024Quote: ... Reverse transcription was performed with the LunaScript RT SuperMix Kit (#E3010, NEB) using the manufacturer’s protocol on a SimpliAmp thermal cycler (Applied Biosystems ...
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bioRxiv - Cancer Biology 2024Quote: Total cellular RNA was isolated using the Monarch Total RNA Miniprep Kit (#T2010, NEB, Ipswich, MA, USA) following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were then transferred into SOC outgrowth media (950 μL, New England BioLabs) at 37 ◦C for 1 hr before expansion into YT growth media (50 mL ...
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bioRxiv - Cancer Biology 2024Quote: BL21 DE3 cells (50 μL, New England Biolabs) were transformed with the pSANG10-3FBG4 plasmid36 (2 μL ...
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bioRxiv - Cancer Biology 2024Quote: ... 20 ng genomic DNA was either treated with exonuclease V (RecBCD, NEB), in a total volume of 30 ul ...
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bioRxiv - Cancer Biology 2024Quote: ... Plasmids were recovered using Monarch Plasmid Miniprep Kit (New England Biolabs #T1010S) or E.Z.N.A ...
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bioRxiv - Cancer Biology 2024Quote: ... and sequencing libraries were generated using the NEBNext® Small RNA Library Prep Set for Illumina® (NEB E7330). Libraries were pooled and sequenced using NextSeq 500 High Output ...
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bioRxiv - Cancer Biology 2024Quote: ... transposed DNA was mixed with NEBNext high-Fidelity 2x PCR Master Mix (NEB, #M0541S), AD1_noMX and AD2.1-2.16 barcoded primers ...
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bioRxiv - Cell Biology 2024Quote: ... The sgRNAs were synthesized by in vitro transcription from DNA oligonucleotide templates using the HiScribe T7 Transcription Kit (New England Biolabs). The transcribed products were subsequently purified using the RNA Clean & Concentrator (ZYMO RESEARCH) ...
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bioRxiv - Cell Biology 2024Quote: Total RNA was extracted from differentiated hiPSC-CMs using the Monarch Total RNA Miniprep Kit (NEB #T2010) and equal amounts of RNA per sample were transcribed into cDNA using the GoScriptTM Reverse Transcription Kit (Promega #A5000) ...
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bioRxiv - Cell Biology 2024Quote: ... The region of interest in the HBEGF locus was amplified using Q5 Hot Start High-Fidelity 2x Master Mix (NEB) with 32 cycles of 98°C for 10 sec ...
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bioRxiv - Cell Biology 2024Quote: ... GFP mRNA was produced in-house using the Hiscribe® T7 Quick high yield RNA synthesis kit and 3’-O-me-m7G cap analog (New England Biolabs, MA, USA) following the manufacture’s protocol ...