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Citations for New England Biolabs :
4851 - 4900 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... polynucleotide kinase (NEB) was used to remove 2’-3’ cyclic phosphate ...
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bioRxiv - Biochemistry 2024Quote: ... A His-Tag encoding sequence was added to the C-terminus of RnfG by using oligonucleotides 5 and 6 via site-directed mutagenesis (Q5® Site-Directed Mutagenesis Kit, NEB, Frankfurt am Main, Germany). The resulting plasmid was checked by sequencing analysis (Microsynth Seqlab ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... The vector and the PCR products were then integrated using Gibson assembly (NEBuilder HiFi DNA Assembly Master Mix, New England Biolabs, #E2621S). Clones were sequenced to verify accuracy ...
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bioRxiv - Neuroscience 2024Quote: ... Genomic DNA was extracted from flies using the Biozym gDNA Kit and PCR amplification performed to assess the deletion in the Pink1 gene using the Q5 High-Fidelity DNA Polymerase (New England Biolabs). Primer sequences ...
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bioRxiv - Genomics 2024Quote: ... T7pol was purchased from NEB (ref. M0274) and used following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... were cloned N-terminally to human CISD1 and point mutations introduced by site-directed mutagenesis using the Q5 Site-Directed Mutagenesis kit (NEB). All plasmids were sequence verified ...
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bioRxiv - Genomics 2024Quote: ... were purchased from New England Biolabs (NEB, references N4040S ...
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bioRxiv - Biochemistry 2024Quote: ... His6-tagged GATE16 was generated via Q5 site-directed mutagenesis PCR (New England Biolabs), replacing the GST tag with the sequence MHHHHHHGS ...
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bioRxiv - Biochemistry 2024Quote: ... This PCR was performed using Q5 high-fidelity DNA polymerase (NEB) with the following settings ...
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bioRxiv - Biochemistry 2024Quote: ... This PCR was performed using Q5 high-fidelity DNA polymerase (NEB) with the following settings ...
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bioRxiv - Biochemistry 2024Quote: All truncations were made by using NEBuilder® HiFi DNA assembly master mix (M5520AVIAL, New England Biolabs, NEB) or using Q5 mutagenesis (E0555L ...
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bioRxiv - Biochemistry 2024Quote: The HIV-1 TAR containing a UUCG loop instead of the CUGGGA loop was produced by in vitro transcription with T7 RNA Polymerase High-Concentration (New England Biolabs) in 40 mM Tris HCl (pH 8) ...
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bioRxiv - Biochemistry 2024Quote: ... via HiFi assembly (New England Biolabs) such that it had an N-terminal Avitag followed by a His6 tag and a C-terminal MBP (pl_JD337) ...
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bioRxiv - Biochemistry 2024Quote: All truncations were made by using NEBuilder® HiFi DNA assembly master mix (M5520AVIAL, New England Biolabs, NEB) or using Q5 mutagenesis (E0555L ...
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bioRxiv - Microbiology 2024Quote: ... used Phusion polymerase (NEB, Phusion High Fidelity DNA Pol, M0530L) for amplification ...
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bioRxiv - Microbiology 2024Quote: ... 100 pmol of POWV_Probe and U6_probe DNA probes were radioactively labeled with 5mCi of [γ32-P] ATP using T4 polynucleotide kinase (NEB) for 2 hours at 37°C ...
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bioRxiv - Microbiology 2024Quote: ... 0.25 μL Q5 High-Fidelity DNA polymerase (New England Biolabs); 5 μL of 5× Q5 High GC Enhancer ...
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bioRxiv - Genomics 2024Quote: ... with NEBNext Multiplex Oligos for Illumina (New England Biolabs). Ampure XP beads were used in all of the bead-based cleanup steps ...
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bioRxiv - Microbiology 2024Quote: ... The variable region V3+V4 of the 16S rRNA gene was amplified using a broad-range primer pair (338F: 5’-ACTCCTACGGGAGGCAGCA-3′, 806R:5′-GGACTACHVGGGTWTCTAAT-3′) using the Phusionâ High-Fidelity PCR Master Mix (New England Biolabs, Beverley, MA). The PCR amplification program was as follows ...
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bioRxiv - Immunology 2024Quote: ... 0.2 µl of Taq DNA Polymerase (NEB, M0267S) to 30 µl of PCR reaction ...
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bioRxiv - Genomics 2024Quote: ... concentration of PhiX174 ssDNA (from NEB) was verified both with a spectrophotometer (Nanodrop ...
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bioRxiv - Biochemistry 2024Quote: ... proteins were eluted with lysis buffer + 250 mM imidazole and bound to chitin-resin (S6651L, NEB), before washing in lysis buffer and overnight incubation with lysis buffer + 200 mM DTT and his-ULP1 protease ...
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bioRxiv - Biochemistry 2024Quote: ... The three amplicons were purified and combined using the NEBuilder HiFi DNA Assembly Master Mix (New England BioLabs, Ipswich, MA, USA) according to the instructions provided by the manufacturer ...
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bioRxiv - Biochemistry 2024Quote: ... Beads were resuspended in 1X CutSmart Buffer (New England Biolabs), and DNA was digested with 20 U ScaI-HF and 20 U Sbf-HF for 1 hour at 37°C ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... between long oligos containing the gRNA target sequences using Q5 polymerase (New England Biolabs, #M0491S). The vector and the PCR products were then integrated using Gibson assembly (NEBuilder HiFi DNA Assembly Master Mix ...
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bioRxiv - Microbiology 2024Quote: ... ONT’s proprietary sequencing adaptors were ligated to the DNA fragments using the Blunt/TA Ligase Master Mix (New England Biolabs) provided in the Ligation Sequencing Kit ...
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bioRxiv - Microbiology 2024Quote: ... Samples were then treated with DNase I and cleaned with the Monarch RNA Cleanup Kit (NEB, Ipswich, MA, USA) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... coli Poly(A) Polymerase (NEB, Ipswich, MA, USA) and cleaned with the Monarch RNA Cleanup Kit (NEB ...
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bioRxiv - Microbiology 2024Quote: ... The DNA was subjected to end-repair and dA-tailing using the NEBNext® Ultra™ II End Repair/dA-Tailing Module (New England Biolabs). Following end-repair and dA-tailing ...
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bioRxiv - Developmental Biology 2024Quote: ... Construction of pUAST- attB-Cic(1-15)A-SBP and pUAST-attB-Cic20A-mVenus was carried out by assembling gene fragments using the HiFi DNA assembly Kit (NEB). Construction of the other subgroups of Cic mutants was done by assembling different parts of Cic generated by overlap PCR using wild type Cic ...
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bioRxiv - Developmental Biology 2024Quote: ... were kindly donated by Philippe Lefebvre and RARα sequences were PCR amplified with primers adding PaqCl cut sites (New England Biolabs, R0745S), cut via PaqCI and ligated into an EF1alpha-mScarlet backbone (95) ...
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bioRxiv - Genomics 2024Quote: ... using the Luna universal qPCR master mix (NEB, ref M3003). Amplifications were run in duplicate on a StepOnePlus real-time PCR system with the following cycling conditions ...
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bioRxiv - Immunology 2024Quote: ... 2 × RNA loading dye (NEB, #B0363S) were added to the system to stop the reaction and the RNA was separated by agarose gel electrophoresis.
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bioRxiv - Genomics 2024Quote: ... custom iTru adapters were ligated to amplicons with NEBNext® Ultra II Ligation Master Mix (Cat No. E7648, NEB, Ipswich, MA) with a 15 min incubation at 20°C (Supplemental Table 8) ...
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bioRxiv - Genomics 2024Quote: NEB VarSkip amplicon libraries were prepared according to the NEBNext® ARTIC SARS- CoV-2 FS Library Prep Kit Workflow for Illumina® (NEB, Cat No. E7658, Ipswich, MA).
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bioRxiv - Genomics 2024Quote: ... was integrated into the cleaved vector via Gibson cloning (New England Biolabs, #E2621L) to produce a retroviral vector that contained either mu6-sgGFP-Cr2 (that harbored no capture sequence ...
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bioRxiv - Genomics 2024Quote: ... Following end repair with NEBNext® End Prep Ultra II Kit (Supplemental Table 9, Cat No. E7645, NEB, Ipswich, MA), custom iTru adapters were ligated to amplicons with NEBNext® Ultra II Ligation Master Mix (Cat No ...
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bioRxiv - Cell Biology 2024Quote: ... in the presence of RNase inhibitor (NEB, M3014) at 37°C for 45 min ...
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bioRxiv - Microbiology 2024Quote: ... Gibson cloning using HiFi assembly mix (New England Biolabs) was used to make pCR3.1-PA-X-NLS-eGFP by amplifying the PR8 sequence of PA-X from pCR3.1-PA-X-myc and the eGFP sequence from pCDNA3.1-eGFP ...
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bioRxiv - Biochemistry 2024Quote: ... coli BL21 (DE3) (New England BioLabs). Selection was carried out on LB agar plates containing kanamycin (50 µg/mL ...
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bioRxiv - Biochemistry 2024Quote: ... 10 U T4 RNA ligase 1 (NEB), 50 mM Tris-HCl pH=7.5 ...
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bioRxiv - Biochemistry 2024Quote: ... 0.2 U/mL yeast inorganic pyrophosphatase (NEB),34 ∼15 µM T7 RNA polymerase ...
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bioRxiv - Biochemistry 2024Quote: ... 25U T7 polymerase (NEB), 2.25mM each of CTP ...
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bioRxiv - Biochemistry 2024Quote: ... and clarified by centrifugation at 16,100 x g for 1hr and incubated with ∼1 mL of pre-equilibrated compact amylose affinity resin beads (NEB). The resin was washed three times with buffer H ...
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bioRxiv - Biochemistry 2024Quote: ... was incubated in a final reaction volume of 200µl containing 1x T7 polymerase reaction buffer (NEB), 25U T7 polymerase (NEB) ...
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bioRxiv - Biochemistry 2024Quote: ... and protein samples were treated with 50-100 units of Quick CIP phosphatase (New England Biolabs, M0525S) overnight for complete dephosphorylation ...
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bioRxiv - Bioengineering 2024Quote: ... Constant regions of target constructs were amplified using Phusion® High-Fidelity DNA Polymerase according to manufacturer recommendations (New England Biolabs, Catalog # M0530L). For small libraries (1- or 2-site libraries ...
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bioRxiv - Biochemistry 2024Quote: Nanobody expression and purification for imaging applications – Nanobodies for imaging were produced in SHuffle® Express (NEB) cells ...
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bioRxiv - Biochemistry 2024Quote: ... Mutagenesis was performed using the Q5 site-directed mutagenesis kit (NEB, Cat#E0552S) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... and cloned into the mammalian expression plasmid pcDNA3.1 using Gibson assembly (NEB). Constructs were validated by Sanger sequencing.