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Citations for New England Biolabs :
4551 - 4600 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2024Quote: ... PCR were performed using 2X Q5 PCR master mix (NEB) and GoTaq master mix for colony PCR and genotyping (Promega ...
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bioRxiv - Microbiology 2024Quote: ... restricted with BsaI (NEB Biolabs) and then ligated with T4 DNA ligase (NEB Biolabs ...
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bioRxiv - Cell Biology 2024Quote: ... polymerase along with template was used for riboprobe synthesis and RNase free DNase (NEB M0303S) was used for template digestion ...
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bioRxiv - Cell Biology 2024Quote: ... PCR product using T7 RNA polymerase (NEB, M0251L) as described previously 18 ...
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bioRxiv - Cell Biology 2024Quote: ... then 1 μL Endo H and 2.5 μL GlycoBuffer 3 (New England Biolabs) was added and incubated for 1 hour at 37°C ...
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bioRxiv - Cancer Biology 2024Quote: ... coli SHuffle cells (NEB) and comprised of the minimal CS-binding region (subunit ID1-ID2a ...
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bioRxiv - Cancer Biology 2024Quote: ... The samples with expected deletion (∼400bp) in the PCR screen were then treated with Shrimp Alkaline Phosphatase and Exonuclease I (New England Biolabs) and sent out for Sanger sequencing (Eurofins Genomics).
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bioRxiv - Cell Biology 2024Quote: ... pUC19*EcorI (NEB), 5’ homology arm-Linker-mAID-3’UTR ...
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bioRxiv - Cell Biology 2024Quote: ... The 3’ homology arm was amplified by PCR (Q5 HiFi polymerase®, NEB), from HCT116 cell genomic DNA using the primers NCAPH2 3’ homology arm forward and reverse ...
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bioRxiv - Cancer Biology 2024Quote: ... or NEBaseChanger (New England Biolabs (NEB)) ...
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bioRxiv - Cell Biology 2024Quote: ... The mutagenesis primers for the addition of the STOP codon at the end of the MPV17 gene from MPV17-HA plasmid (F: 5’-TAATCTAGAATGTACCCATACGATGTTC-3’; R: 5’-GAGCCGATGTGCCTTCCA-3’) were generated using the NEBaseChanger bioinformatic tool (NEB). The mutation was confirmed and the plasmids were validated using Sanger sequencing (Eurofins Discovery).
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bioRxiv - Cell Biology 2024Quote: Site directed mutagenesis was performed using the Q5® Site-Directed Mutagenesis Kit according to the manufacturer’s instruction (New England BioLabs Inc. (NEB), Ipswich ...
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bioRxiv - Cell Biology 2024Quote: Site directed mutagenesis was performed using the Q5® Site-Directed Mutagenesis Kit according to the manufacturer’s instruction (New England BioLabs Inc. (NEB), Ipswich ...
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bioRxiv - Cell Biology 2024Quote: ... All sequences were inserted into L4440 using the NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs) and transformed into DH5α bacteria.
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bioRxiv - Cell Biology 2024Quote: ... Each 1 mL aliquot of microsomes was treated for 10 min at 37°C with 4000 U micrococcal nuclease (New England Biolabs), 2 U RNase-free DNase (Promega) ...
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bioRxiv - Cell Biology 2024Quote: ... 25% of the sample was used for Endo H (New England Biolabs) treatment following manufacturer protocol ...
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bioRxiv - Cell Biology 2024Quote: ... we use the Monarch® Genomic DNA Purification Kit (New England Biolabs) and follow the manufacturer recommendation ...
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bioRxiv - Cancer Biology 2024Quote: ... 1 founder with the L424V was determined by restriction digest with MseI (NEB) and Sanger sequence analysis ...
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bioRxiv - Cancer Biology 2024Quote: ... mRNA magnetic isolation module and sequencing libraries were created using NEBNext Ultra II direction RNA library prep kit for Illumina (New England Biolabs). DNA libraries were validated with TapeStation (Aligent Technologies ...
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bioRxiv - Cancer Biology 2024Quote: ... and Protoscript II reverse transcriptase (New England BioLabs, Inc.), according to manufacturer’s instructions.
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bioRxiv - Cancer Biology 2024Quote: ... template switch Oligo dNTPs (NEB, Cat: No447L), and RNase inhibitor (Life Technologies ...
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bioRxiv - Cancer Biology 2024Quote: ... and NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs) library preparation kit ...
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bioRxiv - Cancer Biology 2024Quote: ... C1-deleted pMYs-CIC::DUX4-IRES-EGFP was cloned from pMYs-CIC::DUX4-IRES-EGFP (a gift from Takuro Nakamura (52) and Michael Kyba (25)) using the Q5 Site-Directed Mutagenesis Kit (NEB E0554S) with the primers described in Supplemental Dataset S2 ...
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bioRxiv - Cancer Biology 2024Quote: ... RNA samples were additionally processed with the Monarch RNA Cleanup Kit (NEB), analyzed for integrity with a TapeStation (Agilent) ...
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bioRxiv - Cancer Biology 2024Quote: ... C1- and HMG-domain deletion and point-mutant constructs were cloned from pcDNA3.1-HA-CIC::DUX4 (a gift from Takuro Nakamura (6)) using the Q5 Site-Directed Mutagenesis Kit (NEB E0554S) with the primers described in Supplemental Dataset S2 ...
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bioRxiv - Cell Biology 2024Quote: ... The ATAC-seq libraries were constructed and amplified using NEBNext High-Fidelity 2x PCR Master Mix (NEB, #M0541) and SYBR Green I (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: Plasmids were transformed in BL21(DE3) competent Escherichia coli (New England Biolabs) or Rosetta(DE3)pLysS E ...
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bioRxiv - Cell Biology 2024Quote: RNA sequencing libraries were prepared using the NEBNext Ultra RNA Library Prep Kit for Illumina following manufacturer’s instructions (NEB, Ipswich, MA, USA). Briefly ...
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bioRxiv - Cell Biology 2024Quote: ... Orange (6X) (New England Biolabs, cat. no. B7022S)
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bioRxiv - Cell Biology 2024Quote: Monarch® Genomic DNA Purification Kit (New England Biolabs, cat. no. T3010S)
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bioRxiv - Cell Biology 2024Quote: DpnI enzyme (New England Biolabs, cat. no. R0176S)
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bioRxiv - Cell Biology 2024Quote: ... ▴CRITICAL For PCR amplification we use the Q5® High-Fidelity DNA Polymerase (NEB) but other can be picked such as Phusion ...
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bioRxiv - Cell Biology 2024Quote: Q5® Hot Start High-Fidelity DNA Polymerase (New England Biolabs, cat. no. M0493)
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bioRxiv - Cell Biology 2024Quote: SwaI enzyme and Neb3.1 buffer (New England Biolabs, cat. no. R0604S)
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bioRxiv - Cell Biology 2024Quote: NEBuilder® HiFi DNA Assembly Master Mix (New England Biolabs, cat. no. E2621S)
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bioRxiv - Cell Biology 2024Quote: ... 1 kb Plus DNA ladder (NEB) was use as ruler and the gel was run for 30 minutes at 130 V ...
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bioRxiv - Cell Biology 2024Quote: 1kb Plus DNA ladder (New England Biolabs, cat. no. N3200S)
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bioRxiv - Cell Biology 2024Quote: Monarch® PCR & DNA Cleanup Kit (New England Biolabs, cat. no. T1030S)
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bioRxiv - Cell Biology 2024Quote: Orange gel loading dye 6X (NEB) was added to the StuI digestion and DpnI treated PCR reactions which were subsequently loaded onto an agarose gel (1% in TAE ...
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bioRxiv - Cell Biology 2024Quote: dNTPs (New England Biolabs, cat. no. N0447S)
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bioRxiv - Cell Biology 2024Quote: StuI enzyme (New England Biolabs, cat. no. R0187S)
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bioRxiv - Cell Biology 2024Quote: ... a homologous region of approximately 800 bp at the 3’ end of the respective gene was amplified without the stop codon from 3D7 gDNA using Phusion high fidelity DNA polymerase (NEB). A homology region of about 400 bp at the 5’ end of the respective gene was used for targeted gene disruption ...
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bioRxiv - Cancer Biology 2024Quote: ... the beads underwent an exonuclease treatment (New England Biolabs M0293L) and second strand synthesis en masse followed by whole transcriptome amplification (WTA ...
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bioRxiv - Cell Biology 2024Quote: ... NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs, Cat# E2621S) was used to construct the donor plasmid containing 450 bp ITSN1 5’ homology arm including start codon ...
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bioRxiv - Cancer Biology 2024Quote: ... ChIP-seq libraries were prepared using NEBNext Ultra II DNA Library Prep Kit (NEB; E7645) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... and NEBNext Ultra II Q5 Master mix (NEB). Amplified ATAC-seq libraries were then size selected with AMPure XP Reagent (Beckman Coulter ...
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bioRxiv - Cancer Biology 2024Quote: ... and subsequently used to assemble the RNA-seq library using NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina (NEB; E7760), according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... Individually barcoded strand-specific libraries for mRNA sequencing were prepared from total RNA samples of high quality (approximately 150 ng per sample) using the NEBNext® RNA Ultra II Directional RNA Library Prep Kit (New England Biolabs) for 12 PCR cycles on the liquid handler Biomek i7 (Beckman Coulter GmbH ...
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bioRxiv - Cell Biology 2024Quote: ... for neuron infection using the NEBuilder HiFi DNA Assembly Cloning Kit (New England Biolabs). iPSC-derived neurons were infected using viral particles diluted in N2B27 media (5 MOI ...
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bioRxiv - Cell Biology 2024Quote: ... supplemented with calf intestinal alkaline phosphatase (Quick CIP, NEB M0525S) to avoid self-ligation ...