Labshake search
Citations for New England Biolabs :
4751 - 4800 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... using ETAA1 DBR R1 RT-PCR primer 5′-AAGTTCTTCTTCTTGACTTTGTGTT-3′ and treated with RNaseH (New England Biolabs, M0297S). 1μl of the cDNA was used for PCR amplification reactions using using GoTaq® G2 DNA Polymerase (Promega ...
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Development of a genetically encoded sensor for probing endogenous nociceptin opioid peptide releasebioRxiv - Neuroscience 2024Quote: ... of the Oprl1 gene were amplified from a C57BL/6 BAC clone by PCR using Q5 Polymerase (New England Biolabs) and cloned into polylinkers of a targeting construct that contained IRES-mnCre:GFP ...
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bioRxiv - Microbiology 2024Quote: ... truncated K227Q (NEB; #M0315S). RNA and RPF samples were each pooled together after PAGE-assisted size selection ...
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bioRxiv - Microbiology 2024Quote: ... and ligated into the pMAL-p2E vector digested with the same restriction endonucleases using the T4 DNA Ligase (New England Biolabs) according to the manufactureŕs instructions ...
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bioRxiv - Microbiology 2024Quote: ... Ligation of 5’ linker using T4 RNA ligase 1 (NEB; #M0204S) was conducted after phosphorylating the 5’ end of the pooled fragments with T4 PNK (NEB ...
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bioRxiv - Microbiology 2024Quote: ... and mixed with 250 μl of Amylose resin (New England Biolabs) for 2 hours at 4°C in an orbital shaker ...
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bioRxiv - Microbiology 2024Quote: ... The plasmid was transformed into BL21 (DE3) competent cells following the manufacturer’s protocol (NEB), a single colony was inoculated into 10 mL LB ampicillin (10 mg/ml ...
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bioRxiv - Microbiology 2024Quote: ... 10^106 of DMSO or IMP-1088 released virions were treated with two-fold dilutions of Furin (New England Biolabs, Cat. no. P8077L) in 20 mM HEPES ...
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bioRxiv - Microbiology 2024Quote: ... SARS-CoV-2-specific amplicons were generated using xGen Artic V4 NCoV-2019 primers (Integrated DNA Technologies Inc., Coralville, Iowa) and Q5 Hot Start High Fidelity PCR Master Mix (New England Biolabs, Cat. no. E0555S). Sequencing libraries were prepared using NEBNext ultra II DNA library kit (New England Biolabs ...
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bioRxiv - Microbiology 2024Quote: ... Sequencing libraries were prepared using NEBNext ultra II DNA library kit (New England Biolabs, Cat. no. E7805S) and unique dual indexes (Integrated DNA Technologies Inc. ...
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bioRxiv - Microbiology 2024Quote: ... RNA concentration and quality were evaluated using NanoDrop 2000 Spectrophotometer (Thermo Fischer Scientific) and reverse-transcribed to cDNA using LunaScript® RT SuperMix Kit (New England Biolabs, Cat no. E3010) with random hexamers and oligo dT ...
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bioRxiv - Immunology 2024Quote: ... Stranded RNA sequencing libraries were prepared as described using the NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (Cat# E7760L; New England Biolabs). Purified libraries were qualified on an 4150 TapeStation (Agilent Technologies ...
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bioRxiv - Bioengineering 2024Quote: ... transfected MCF7 cells were harvested using Bio-TRI® (BioLabs) and RNA was extracted following manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... Unique dual index primer pairs (New England Biolabs) were used to barcode single -cell libraries ...
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bioRxiv - Cancer Biology 2024Quote: Total RNA was collected from parental iOvCa147 cells and DYRK1A-/-cells from 24-hour adherent or 6-hour spheroid conditions using the Monarch Total RNA Miniprep Kit (NEB #T2010S) as described above ...
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bioRxiv - Cancer Biology 2024Quote: ... RNA libraries were prepared using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs). These libraries were subsequently sequenced for paired-end reads of 2×100 bp on an MGI system at a sequencing depth of 50X.
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bioRxiv - Cancer Biology 2024Quote: Isolated total RNA was processed using the NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs, MA, USA) to isolate intact poly(A)+ RNA following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... with 1 µg of total RNA used for sequencing library construction with NEBNext Ultra RNA Library Prep Kit for Illumina (NEB, USA). Raw FastQ files were mapped using Bbsplit from the Bbtools 39.01 suite against Mm10 and hg38 (31 ...
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bioRxiv - Physiology 2024Quote: ... The insoluble matrix samples were resuspended in in 1.5x Laemmli buffer with DTT (New England Biolabs) in a normalized volume based on cytosolic protein concentration (50 μl per mg of soluble protein) ...
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bioRxiv - Plant Biology 2024Quote: ... 75mer oligonucleotides comprising the amiRNAs and SisRNAs sequences were cloned into the BsaI-sites by Golden Gate strategy using BsaI-HF®v2 (NEB, R3733) and T4 DNA Ligase (Thermo Fischer Scientific ...
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bioRxiv - Synthetic Biology 2024Quote: ... Plasmid assembly was performed using NEBuilder HiFi DNA Assembly Master Mix (NEB), followed by transformation into NEB 5-alpha competent E ...
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bioRxiv - Synthetic Biology 2024Quote: ... DNA amplification for plasmid construction was performed using Q5 Hot Start High-Fidelity 2x Master Mix (NEB), and colony PCR was performed using Phire Green Hot Start II PCR Master Mix (Thermo Fisher Scientific) ...
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bioRxiv - Systems Biology 2024Quote: ... These PCR products were used for Gibson assembly with the barcoded promoters (#E2611S; NEB). Gibson assembly reactions were purified with magnetic beads and 2 µl of the reaction were electroporated into 20 µl of electrocompetent e ...
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bioRxiv - Systems Biology 2024Quote: ... and barcode-insert fragments were linearized again with I-SceI (#R0694S; NEB). Barcode-insert fragments were then amplified by PCR with Illumina adapters and sequenced on an Illumina NextSeq 550 platform using 150 bp paired-end sequencing ...
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bioRxiv - Plant Biology 2024Quote: ... The RFP insert was digested from the pBeaconRFP_GR vector (https://gatewayvectors.vib.be/index.php/ ID: 3_20, Bargmann & Birnbaum, 2009) using the restriction enzyme NdeI (NEB, R0111S) and further ligated into the NdeI site of the pUC18-AtMIR390a-B/c vector ...
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bioRxiv - Biochemistry 2024Quote: ... was ligated using T4 DNA ligase (NEB, USA) into the pET-26b vector cleaved with the same enzymes ...
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bioRxiv - Biochemistry 2024Quote: ... These fragments were cloned into linearized pUC19 using the Hi-Fi Assembly Cloning Kit (New England Biolabs). The tps1Δ mutant was confirmed by PCR and the TPS1IDDΔ strain was confirmed by PCRs and sequencing to confirm the replacement of the CnTps1IDD with the CaTps1IDD ...
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bioRxiv - Microbiology 2024Quote: ... Repair template was generated by using oligos P7/P8 to amplify GFP sequence from plasmid pCas9/sgRNA/Bleo with Phusion® High-Fidelity DNA Polymerase (New England Biolabs; #M0530S). ME49Δku8023 was transfected with 100 μg of gRNA and 20 μg of repair template ...
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bioRxiv - Microbiology 2024Quote: ... using Q5® Site-Directed Mutagenesis Kit (New England Biolabs; #E0554S). Guide RNA and repair template sequences are listed in Supplementary Data 3c.
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bioRxiv - Microbiology 2024Quote: ... digested with BamHI and PstI restriction endonucleases according to the manufactureŕs instructions (New England Biolabs), and ligated into the pMAL-p2E vector digested with the same restriction endonucleases using the T4 DNA Ligase (New England Biolabs ...
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bioRxiv - Biophysics 2024Quote: ... 25μg of the plasmid containing the hsp70 promoter sequence was digested with HindIII restriction enzyme (NEB R3104) for 3 hours at 37C ...
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bioRxiv - Biophysics 2024Quote: ... followed by Amylose resin (NEB cat. # E8021S) and heparin affinity column (Cytiva cat ...
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bioRxiv - Biophysics 2024Quote: ... and AfeI (NEB; 10,000 units/mL) instead ...
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bioRxiv - Biophysics 2024Quote: ... but employed restriction endonucleases DrdI (NEB; 10,000 units/mL) and AfeI (NEB ...
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bioRxiv - Biophysics 2024Quote: ... A 1 kbp ladder (NEB) was used for the relative comparison of the bands.
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bioRxiv - Biophysics 2024Quote: ... and in 1 × CutSmart buffer (NEB). The reaction mixture was pipetted ten times to ensure thorough mixing ...
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bioRxiv - Biophysics 2024Quote: ... In the first double-digestion reaction two DNA fragments of 3,621 nt (D) and 3,628 nt (D’) were generated using DraIII and BaeGI enzymes (New England Biolabs; Figure S1a). The digestion reaction involved annealing two oligonucleotides (Figure S1b ...
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bioRxiv - Biophysics 2024Quote: ... the mixture was purified using the Monarch PCR and plasmid DNA purification kit (NEB), eluted in 10 mM Tris-HCl pH 8.0 and stored at -20 °C for further experiments ...
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bioRxiv - Biophysics 2024Quote: ... 55 The samples were mixed with 6 × purple loading dye without sodium dodecyl sulfate (NEB) and with 10 × TBE to make a 1 × solution ...
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bioRxiv - Biochemistry 2024Quote: ... the ecpga gene (GenBank: X04114.1) was amplified by PCR using Q5 polymerase (NEB, USA) from our previous plasmid pKA1868 with ecPGA cloning primers bearing NdeI and XhoI site overlaps ...
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bioRxiv - Biochemistry 2024Quote: ... and digested PCR product by NdeI and XhoI restriction enzymes (NEB, USA) was ligated using T4 DNA ligase (NEB ...
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bioRxiv - Biochemistry 2024Quote: ... The parental vector was digested by the DpnI enzyme (NEB, USA), and the ligation mixture was directly electroporated into E ...
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bioRxiv - Biochemistry 2024Quote: ... These fragments were cloned into linearized pUC19 using the Hi-Fi Assembly Cloning Kit (New England Biolabs). The plasmids carrying the desired inserts were transformed into strains by biolistic transformation as previously reported (82) ...
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bioRxiv - Biophysics 2024Quote: ... and T4 ligation (NEB), and the resulting plasmid was subjected to a round of site directed mutagenesis (NEB ...
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bioRxiv - Cancer Biology 2024Quote: ... 1X Non-Essential Amino Acids (NEAA, #SH30238.01, Nordic Biolabs), 2mM L-Serine (#S4311 ...
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bioRxiv - Cell Biology 2024Quote: ... using Luna Universal qPCR Master Mix (NEB, M3003L). Sequences of all primers used can be found in Suppl ...
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bioRxiv - Cell Biology 2024Quote: INS-1 832/3 SNAP/FLAG-hGLP-1R WT or V229A cells were seeded onto glass bottom MatTek dishes and labelled with SNAP-Surface 647 (S9136S, New England Biolabs) for 15 minutes at 37°C in full media ...
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bioRxiv - Cell Biology 2024Quote: ... and inserted between the SgrAI and MfeI sites present at the C-terminal end of mCherry (restriction enzymes provided by NEB). Based on the FL plasmid ...
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bioRxiv - Biochemistry 2024Quote: ... Phusion® High-Fidelity DNA Polymerase was obtained from NEB (New England Biolabs, M0530S). Glycine was obtained by VWR (1042011000) ...
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bioRxiv - Biochemistry 2024Quote: ... followed by fragment assembly using Gibson Assembly Master Mix (NEB). Kits were used according to manufacturer’s instructions.