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Citations for New England Biolabs :
4451 - 4500 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... and then quantified by qPCR (NEBNext Library Quant Kit, NEB, E7630). 20 pM of DNA were loaded onto an Illumina MiSeq (v3 ...
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bioRxiv - Neuroscience 2024Quote: ... The enriched mRNA was fragmented into short fragments using fragmentation buffer and reverse transcribed into cDNA using the NEBNext Ultra RNA Library Prep Kit for Illumina (NEB #7530 ...
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bioRxiv - Neuroscience 2024Quote: ... Using Gibson Assembly (NEBuilder® HiFi DNA Assembly Master Mix, New England Biolabs, E2621), we used CamKII-LGI1-pH (Addgene Plasmid #185537 ...
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bioRxiv - Neuroscience 2024Quote: ... 0.4 mM dNTP mix (New England Biolabs, N0447L), 0.4 U/μL RNaseOUT (Invitrogen ...
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bioRxiv - Neuroscience 2024Quote: ... Sequencing libraries were amplified using NEBNext HiFi 2x Master Mix (NEB) as follows ...
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bioRxiv - Neuroscience 2024Quote: ... Sequencing libraries were prepared using the NEBNext Ultra II Directional RNA Library Prep Kit (New England BioLabs, Ipswich, MA) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... The enriched mRNA was fragmented into short fragments using fragmentation buffer and reverse transcribed into cDNA using the NEBNext Ultra RNA Library Prep Kit for Illumina (NEB #7530, New England Biolabs, Ipswich, MA, USA). The resulting double-stranded cDNA fragments were subjected to end repair ...
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bioRxiv - Neuroscience 2024Quote: ... Amplicons were purified from PCR buffer using Monarch PCR cleanup kit (New England Biolabs) and submitted for Sanger sequencing (EtonBio).
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bioRxiv - Neuroscience 2024Quote: ... and used to form ribonucleoprotein (RNP) complexes when combined with recombinant Cas9 protein (New England Biolabs). Ribonucleoprotein complexes were formed using the method of Kouranova49 ...
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bioRxiv - Neuroscience 2024Quote: ... Genomic region containing the CRISPR-edit was PCR-amplified using OneTaq polymerases in Standard Buffer (New England Biolabs). After PCR ...
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bioRxiv - Neuroscience 2024Quote: ... The RNA library preparation was performed following the NEB NEBNext Ultra II Directional RNA Library Preparation protocol (New England Biolabs Inc., Ipswich, MA, USA). RNA libraries were loaded on a Bioanalyzer 2100 DNA High Sensitivity chip to check for size ...
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bioRxiv - Neuroscience 2024Quote: ... Ribosomal RNA was removed using the rRNA Depletion kit (NEB) and multiplexed RNA-seq libraries were generated using the NEBNext Ultra Directional RNA library Prep Kit for Illumina (NEB) ...
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bioRxiv - Neuroscience 2024Quote: ... 100ng input RNA was used for library preparation with the NEBNext Globin & rRNA depletion kit (New England Biolabs, E7750X) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... 4U/ml Proteinase K (NEB, Ipswich, MA), 50μg/ml RNase A (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... 2006) and introduced into the expression vector pET22b using the restriction sites NdeI and SalI (New England Biolabs). A His6-tag-STOP sequence was introduced into the SalI-reverse primer to enable affinity purification via immobilized metal affinity chromatography (IMAC) ...
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bioRxiv - Neuroscience 2024Quote: ... Pellets were resuspended in NEB buffer prior to centrifugation at 11,500 rpm for 20 minutes at 4°C on a sucrose gradient (1.6M sucrose in NEB and 0.8M sucrose in NEB). Nuclei-containing pellets were fixed with 0.3% formaldehyde in NEB and rotated at room temperature for 10 minutes ...
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bioRxiv - Neuroscience 2024Quote: ... mixed with Nuclear Extraction buffer (NEB) (10mM Tris-HCl pH 8.0 ...
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bioRxiv - Molecular Biology 2024Quote: ... and the D-AlaR cassette (pGGF003) were combined in a ’Greengate reaction’ using BsaI-HF (NEB), T4 DNA-Ligase (Thermo Scientific) ...
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bioRxiv - Molecular Biology 2024Quote: ... and NEBuilder DNA HIFI Assembly Master Mix (NEB, 2621) were used to combine vectors and inserts ...
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bioRxiv - Molecular Biology 2024Quote: ... PCR fragments were amplified using Thermo Scientific Phusion High-Fidelity DNA Polymerase (NEB) and ligated into entry vectors through BsaI-restriction cloning ...
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bioRxiv - Molecular Biology 2024Quote: ... with LdG_IF primers and inserted into EcoRI and NcoI-HF (NEB) digested pLG plasmid by In-Fusion® HD Cloning Kit (Takara ...
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bioRxiv - Molecular Biology 2024Quote: ... The dsDNA was subsequently labelled with T4 polynucleotide kinase (NEB) and γ-32P-ATP ...
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bioRxiv - Neuroscience 2024Quote: ... or SpeI (New England Biolabs, Cat#: 50811989) restriction enzyme digestion to generate antisense templates ...
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bioRxiv - Molecular Biology 2024Quote: ... or 2μg (dual-guide) per well were set up using the Q5 Hot Start High-Fidelity 2× Master Mix (NEB #M0494) in a total volume of 50μl ...
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bioRxiv - Microbiology 2024Quote: ... restricted with BsaI (NEB Biolabs) and then ligated with T4 DNA ligase (NEB Biolabs ...
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bioRxiv - Microbiology 2024Quote: Site-directed mutagenesis was performed by the Q5 site-directed mutagenesis kit (NEB) with primers designed to harbor the mutation site(s) ...
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bioRxiv - Molecular Biology 2024Quote: ... poly(A) selection was performed using Poly(A) mRNA Magnetic Isolation Module (NEB, E7490) with 500 ng of total RNA as input ...
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bioRxiv - Microbiology 2024Quote: ... Amplicons were then PCR purified using a Monarch® PCR & DNA cleanup kit (NEB-T1030S) once a band of the correct size was visualized on a 1% Agarose gel ...
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bioRxiv - Molecular Biology 2024Quote: ... The backbone was digested with BamHI (NEB) and subsequently treated with Antarctic phosphatase (NEB ...
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bioRxiv - Molecular Biology 2024Quote: ... and cloned into pHAGE2- EF1a-Multiple Cloning Site-IRES-PuroR plasmids using NotI and BamHI restriction enzymes (New England Biolabs), as described previously39 ...
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bioRxiv - Molecular Biology 2024Quote: ... KRAB-MECP2 and dTomato were amplified and cloned into the AAVS1 targeting vector described above with the NEBuilder® HiFi DNA Assembly Master Mix (NEB, #M5520). For the PEmax and PEmax-MLHdn1 plasmid the CRISPRi plasmid was used as a backbone while inserting the PEmax or PEmax-P2A-MLHdn1 (Addgene ...
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bioRxiv - Molecular Biology 2024Quote: The point and deletion mutants of SMG8 were PCR amplified using Q5 polymerase (NEB) and inserted with an N-terminal FLAG-tag via NheI and NotI restriction sites into the tetracycline-inducible pcDNA5/FRT/TO vector (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2024Quote: ... the EGFP in the original pAlpha.SIN.SF.EGFP.wPRE. plasmid (Suerth et al. 2012) was exchanged for the d2GFP by digestion with NcoI and SpeI (NEB) restriction enzymes ...
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bioRxiv - Microbiology 2024Quote: The plasmid pCN51F was prepared by cleaving the plasmid pCN5160 with the restriction enzymes SphI and NarI (New England Biolabs) and ligating it with annealed oligonucleotides carrying a Cerulean terminator ...
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bioRxiv - Microbiology 2024Quote: ... Ligation with gentamycin cassette PCR amplified from the plasmid pACEBac1 (GenevaBiotech) was performed O/N at 16 °C using T4 DNA Ligase (NEB). Competent E ...
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bioRxiv - Microbiology 2024Quote: ... treated with Shrimp Alkaline Phosphatase (NEB) and purified by agarose gel electrophoresis ...
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bioRxiv - Synthetic Biology 2024Quote: ... ∼0.5 ng of plasmid DNA was combined with 2.5 µL of rCutSmart™ (New England Biolabs #B6004), 5 units of enzyme ...
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bioRxiv - Genomics 2024Quote: ... 0.4 µM letter-representing DNA and 40 U/µl Quick ligase enzyme (NEB, M2200S) for 1 hour at room temperature in dark ...
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bioRxiv - Genomics 2024Quote: ... murine (NEB, M0314S), 600 units/ml RNasin® Plus RNase Inhibitor (Promega ...
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bioRxiv - Genomics 2024Quote: ... TruSeq-barcoded RNAseq libraries were generated with the NEBNext Ultra II Non-Directional RNA Library Prep Kit (New England Biolabs). Each library was quantified with a Qubit (dsDNA HS kit ...
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bioRxiv - Genomics 2024Quote: ... We then incubated the cells with phosphatase buffer composed of 1x rCutsmart buffer (NEB, B6004S), 0.1 % TX-100 ...
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bioRxiv - Genomics 2024Quote: ... and 20 mM DTT (NEB, 7016L), both of which have only been freeze-thawed once ...
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bioRxiv - Genomics 2024Quote: ... 0.122 U/μL RNase H (New England Biolabs) was added and the reaction was incubated at 37 °C for 40 min before the RNA – internal bridge adapter ligation step following a published protocol (12).
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bioRxiv - Genomics 2024Quote: ... 250 U/ml quick CIP enzyme (NEB, M0525S) for 20 min at 37 °C to remove any free 5’ phosphate groups that may have formed due to stray light and washed the samples with 1x PBS once and nuclease-free water once ...
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bioRxiv - Evolutionary Biology 2024Quote: ... gBlocks fragments (Integrated DNA Technologies) were ordered with overhangs to facilitate HiFI assembly (New England Biolabs; E2621). The codon optimized RGECO1 was cloned into the S ...
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bioRxiv - Cell Biology 2024Quote: ... Site directed mutagenesis was performed according to the manufacturer’s instructions to introduce gRNA target-site mutations in wild-type Pcyt2 using the Q5 Site-Directed Mutagenesis Kit (New England Biolabs, E0554). Primers were designed using NEBaseChangerv1.
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bioRxiv - Evolutionary Biology 2024Quote: ... The assembled plasmid was transformed into NEB 5-alpha (high efficiency) competent cells (New England Biolabs; C2987). After verifying the sequence ...
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bioRxiv - Genomics 2024Quote: ... PolyA+ RNA was then isolated with the NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs). TruSeq-barcoded RNAseq libraries were generated with the NEBNext Ultra II Non-Directional RNA Library Prep Kit (New England Biolabs) ...
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bioRxiv - Genomics 2024Quote: ... The ATAC libraries were amplified for 11 cycles using NEBNext 2X MasterMix and Nextera Index primers (New England Biolabs, # M0541S). The amplified libraries were size selected using AMPure beads (Beckman Coulter ...
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bioRxiv - Synthetic Biology 2024Quote: ... and EcoRI-HF (New England Biolabs # R3101), respectively ...