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Citations for New England Biolabs :
4301 - 4350 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... and used this as input for library generation using the NEBNext® Ultra™ II RNA Library Prep (NEB). The quality and concentration of the cDNA library was assessed using the BioAnalyzer 2100 high sensitivity DNA kit ...
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bioRxiv - Biochemistry 2024Quote: ... We enriched for mRNA using the NEBNext® Poly(A) mRNA Magnetic Isolation Module (NEB) and used this as input for library generation using the NEBNext® Ultra™ II RNA Library Prep (NEB) ...
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bioRxiv - Biochemistry 2024Quote: ... The protein was digested by adding 2 µL of PK (NEB) to the mixture and incubating at 45°C for 45 min ...
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bioRxiv - Biochemistry 2024Quote: ... Purified DNA was treated with 100 nM TDP2 in 30 µL of 1X rCutsmart buffer (NEB) and incubated at 37°C for 180 min to completely remove the 5’ phosphotyrosine adducts ...
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bioRxiv - Biochemistry 2024Quote: ... dNTPs (New England Biolabs, N0446S), N,N-Dimethyl formamide anhydrous (Sigma ...
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bioRxiv - Biochemistry 2024Quote: ... with C-terminal TEV, GFP, 7xHis, and StrepII (Thawani et al., 2020) using the Q5 Site Directed Mutagenesis Kit (NEB). All three constructs were expressed using the Bac-to-Bac Sf9 cell expression system (Invitrogen) ...
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bioRxiv - Biochemistry 2024Quote: ... Reactions were stopped by adding 2µl of stop buffer (10mg.ml-1 proteinase K (NEB), 1% SDS ...
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bioRxiv - Genomics 2024Quote: ... clone C36B11 (NEB, 9733S) was used at 1:200 dilution ...
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bioRxiv - Genomics 2024Quote: ... 2 mg/mL temperature labile proteinase K (NEB, P8111S), and 12.5 mM MgCl2 for RNA fragmentation) ...
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bioRxiv - Genomics 2024Quote: ... 1 μL T4 RNA ligase reaction buffer (NEB), 1 μL RNAseOUT (Invitrogen) ...
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bioRxiv - Genomics 2024Quote: ... 30 μg per replicate of poly-adenylated mRNA was isolated using oligo d(T) beads (NEB E7490L). Resulting mRNA was split into 6 separate reactions and cDNA was synthesized using a gene-specific primer (5’-CTCATCAATGTATCTTATCATGTCTG-3’ ...
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bioRxiv - Genomics 2024Quote: ... 5 μg of HMW DNA sample was fragmented by sonication to a size of 350 bp and sequencing library was prepared using NEB Next® Ultra™ DNA Library Prep Kit for Illumina (New England Biolabs, USA) following manufacturer’s instruction ...
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bioRxiv - Genomics 2024Quote: ... and amplified with 14 cycles of PCR using LongAmp Taq Master Mix (New England Biolabs, UK). cDNA was quantified using the Qubit DNA High sensitivity assay (Invitrogen ...
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bioRxiv - Genomics 2024Quote: ... Supplementary Table 1) using a modified protocol of the NEBNext Single Cell/Low Input cDNA Synthesis & Amplification Module (New England Biolabs, UK) (Supplementary Methods and Supplementary Table 18 ...
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bioRxiv - Plant Biology 2024Quote: ... 1.5 µl of CutSmart Buffer (NEB), 10X Bovin Serum Albumin (1.5 µl ...
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bioRxiv - Microbiology 2024Quote: ... and OneTaq polymerase (NEB, OneTaq Quick-Load DNA polymerase ...
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bioRxiv - Biochemistry 2024Quote: ... 30 μL of Protein G Magnetic beads (NEB) was mixed with 1 μL of anti-RelA antibody (Diagenode ...
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bioRxiv - Genomics 2024Quote: All adapter-ligated amplicons were amplified with custom iTru5 and iTru7 index primers (Glenn et al. 2019) with NEBNext Ultra II Q5 Master Mix (NEB, Cat No, M0544, Ipswich, MA) according to reaction conditions described in Supplemental Table 6 ...
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bioRxiv - Genomics 2024Quote: ... 1× phi29 buffer (New England Biolabs), 4 mM deoxynucleoside triphosphates (Invitrogen) ...
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bioRxiv - Synthetic Biology 2024Quote: ... PCR were performed using 2X Q5 PCR master mix (NEB) and GoTaq master mix for colony PCR and genotyping (Promega ...
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bioRxiv - Biophysics 2024Quote: ... Mfel and Smal (New England Biolabs), respectively ...
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bioRxiv - Cancer Biology 2024Quote: ... The resulting mixture was transformed into competent DH5α bacteria (NEB, C2987I), and the plasmid was amplified and purified using the Nucleobond Xtra Midi kit (Macherey-Nagel ...
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bioRxiv - Biochemistry 2024Quote: ... using Qubit DNA HS kit (New England Biolabs, M0494S). The final RP libraries were single-end sequenced with a NextSeq2000 P2 system (Illumina) ...
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bioRxiv - Cancer Biology 2024Quote: All the newly generated plasmids reported in this study were created by Gibson Assembly using NEBuilder HiFi DNA Assembly Master Mix (New England Biolabs, E2621L) as per manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... 0.2 mM dNTP (NEB®, #N0447L), 2.5 ng/µL forward primer ...
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bioRxiv - Biochemistry 2024Quote: ... coli strain T7 Express lysY/Iq (New England Biolabs, Ipswich, MA, USA) and purified by Ni- ...
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bioRxiv - Synthetic Biology 2024Quote: ... interORF and ORF2p were then assembled using Hifi Assembly mix (NEB) into pCMV vector with the CMV promoter region removed ...
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bioRxiv - Microbiology 2024Quote: ... respectively) using T4 RNA Ligase 1 (New England Biolabs, USA; #M0204) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... FOX templates were obtained from Addgene 45 and FOX-DBD DNA sequences were ligated and cloned into the pGEX-6P2 using Gibson Assembly Master Mix (NEB: E2611L). Point mutants were generated with the QuikChange Lightning site-directed mutagenesis kit (Agilent ...
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bioRxiv - Biochemistry 2024Quote: ... a codon-optimized synthetic gene product was synthesized in two overlapping halves by Twist Biosciences and integrated into a vector using the NEBuilder HiFi DNA Assembly Cloning Kit (NEB). pNH-TrxT was a gift from Opher Gileadi (Addgene plasmid # 26106 ...
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bioRxiv - Biochemistry 2024Quote: ... in Cas9 nuclease reaction buffer (NEB) were incubated for 10 minutes at 25°C in the absence or presence of 1 mM EoHR (1% DMSO ...
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bioRxiv - Biochemistry 2024Quote: ... coli RNA polymerase core enzyme (New England Biolabs) as the analyte ...
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bioRxiv - Biochemistry 2024Quote: ... and OneTaq DNA polymerase (NEB) was used for general analytical PCR ...
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bioRxiv - Biochemistry 2024Quote: ... Qiagen protocol 4 was followed for enzymatic lysis with lysozyme (DOT Scientific catalog #DSL38100) and 20 µL proteinase K (NEB catalog #P8107S). Qiagen protocol 7 was followed for RNA purification including DNase I digestion on-column (Qiagen catalog# 79254) ...
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bioRxiv - Biochemistry 2024Quote: ... 0.5 µL Q5 High-Fidelity DNA Polymerase (NEB, M0491S), 10 µL 5 X Q5 reaction buffer ...
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bioRxiv - Biochemistry 2024Quote: ... followed by addition of 1.5 U of EcoRV (NEB), and further incubation of the mixture for 15 min at 37 °C ...
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bioRxiv - Biochemistry 2024Quote: ... amylose resin (NEB), or glutathione agarose (GE Healthcare) ...
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bioRxiv - Biochemistry 2024Quote: ... EU-labeled cells fixed and permeabilized as above were incubated in 1×CutSmart buffer (NEB) with 4ng/µL RNase A or 5U/µL benzonase ...
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bioRxiv - Biochemistry 2024Quote: ... The high-fidelity Q5 Polymerase enzyme (NEB) was used to amplify the whole plasmid length using the specific site-directed mutagenesis (SDM ...
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bioRxiv - Biochemistry 2024Quote: ... 1 unit of Phusion DNA Polymerase (NEB), as well as 10 pg of a pD-template ...
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bioRxiv - Biochemistry 2024Quote: ... and T.el4h-O.i.I plasmid was linearized using an engineered (BamHI for O.i. and O.i.-TPP and HindIII for T.el4h-O.i.) restriction site (NEB). 50 μg of template DNA was added to a total volume of 1 mL of in vitro transcription buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Genomics 2024Quote: ... 1% SDS) and 20 μl of Proteinase K (NEB, cat. no. P8107S). Subsequently ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 5μl of the PCR product was mixed with 6x loading buffer (New England BioLabs) and electrophoresed on a 1.5% TAE agarose gel for F1534C and V410L ...
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bioRxiv - Genetics 2024Quote: ... Gibson Assembly was performed using the master mix (New England Biolabs, NEB) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2024Quote: ... Gibson Assembly was performed using the master mix (New England Biolabs, NEB) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2024Quote: ... and PvuII-HF (NEB Cat#R3151S). Each K3 allele (K3-WT and K3-H47R ...
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bioRxiv - Genetics 2024Quote: ... NEBridge Ligase Master Mix (NEB #M1100), NEB PaqCI and PaqCI activator (NEB #R0745 ...
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bioRxiv - Genetics 2024Quote: ... Up to 1µg was the used for reverse transcription using the ProtoScript Reverse Transcription kit (NEB). qPCR was done using iTaq universal SYBR Green Supermix (BioRad ...
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bioRxiv - Microbiology 2024Quote: ... and BamHI-HF (NEB, #R3136L). All site directed mutagenesis was performed using the NEBuilder HiFi DNA Assembly Master Mix according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... The RT reaction was diluted 2-fold with nuclease free water and 1 μl of the diluted mix was subjected to 5’ RACE PCR using Q5 polymerase (NEB) with a touchdown PCR protocol ...