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Citations for New England Biolabs :
4251 - 4300 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... All PCR-generated probes were treated with exonuclease I (NEB) according to the manufacturer’s protocol to remove single-stranded DNA ...
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bioRxiv - Microbiology 2024Quote: ... the NEBNext Ultra II DNA Library Prep Kit (NEB) was used according to the manufactu er’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... and the radiolabeled 3’ fragment was ligated to an unlabeled 5’ fragment by splinted ligation with T4 DNA ligase (NEB) at 30°C for 2 h ...
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bioRxiv - Plant Biology 2024Quote: ... To obtain CRISPR-Cas9 mutant alleles of RELK1 one guide RNA specific for exon 2 was cloned into pBUE411 by HiFi cloning (NEB). The resulting construct was introduced into Hi-II immature embryos by Agrobacterium-mediated transformation ...
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bioRxiv - Plant Biology 2024Quote: To obtain CRISPR-Cas9 mutant alleles of RELK2 and RELK3 a dual targeting guide RNA specific for exon 2 was cloned into pBUE411 by HiFi cloning (NEB). To obtain CRISPR-Cas9 mutant alleles of RELK1 one guide RNA specific for exon 2 was cloned into pBUE411 by HiFi cloning (NEB) ...
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bioRxiv - Microbiology 2024Quote: ... illumina sequencing libraries were prepared with a NEBNext®Ultra™DNA Library preparation kit (New England Biolabs, USA) of 500 mg DNA samples from each sample ...
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bioRxiv - Microbiology 2024Quote: ... DNA was digested with Ncol-HF (New England BioLabs, Notting Hill, Australia). Real-time PCR reactions were performed using RT2 Forget-Me-NotTM EvaGreen qPCR Mastermix (Biotium ...
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bioRxiv - Genetics 2024Quote: ... 5ug of genomic DNA was dephosphorylated in a 30ul reaction volume using 15U of Quick calf intestinal phosphatase (NEB cat #M0525) and 3ul of 10x rCutsmart Buffer (NEB B6004S) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... We used the Monarch PCR and DNA Cleanup Kit (NEB) to purify PCR products ...
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bioRxiv - Evolutionary Biology 2024Quote: ... NEBuilder HiFi DNA Assembly Master Mix and T4 DNA ligase from New England Biolabs (NEB). We obtained oligonucleotides from Sigma-Aldrich ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 7.5 ng of plasmid backbone and 2.5 µl NEBuilder HiFi DNA Assembly Master Mix (NEB). We incubated the reaction mix for 16 h at 50 °C ...
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bioRxiv - Evolutionary Biology 2024Quote: ... genomic DNA was extracted from each homogenized pool of 100 flies using the Monarch Genomic DNA Purification Kit (New England Biolabs). DNA quality was assessed using Nanodrop and quantified with Qubit ...
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bioRxiv - Genomics 2024Quote: ... Illumina TruSeq adapters were ligated to DNA fragments in a reaction containing 2X Quick ligation buffer (NEB), 0.25 μM of adapter and 2 μL of Quick ligase (NEB ...
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bioRxiv - Genomics 2024Quote: ... 0.25 μM of adapter and 2 μL of Quick ligase (NEB) for 20 minutes at 23°C in 40 μL ...
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bioRxiv - Genomics 2024Quote: ... 0.2 mM of dATPs and 7.5 U of Klenow fragment-exo (NEB) at 37°C for 60 minutes in 25 μL ...
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bioRxiv - Genetics 2024Quote: ... Plasmid templates were eliminated using DpnI treatment (NEB R0176) prior to the ssDNA synthesis..
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bioRxiv - Genetics 2024Quote: ... Homology arms were obtained by PCR from HEK-293T genomic DNA and a plasmid template was generated per candidate GSH site by Gibson assembly (New England Biolabs) using fragments synthesized by Q5 High Fidelity Taq polymerase (New England Biolabs) ...
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bioRxiv - Genetics 2024Quote: ... phosphorylation by T4 polynucleotide kinase (NEB M0201S), and ligated with T4 DNA ligase (NEB M0202 ...
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bioRxiv - Genetics 2024Quote: ... Enzymes utilized were obtained from NEB, and cloning as well as DNA extraction kits were sourced from Zymo ...
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bioRxiv - Evolutionary Biology 2024Quote: ... After the reaction we purified the PCR product with Monarch PCR and DNA Cleanup Kit (NEB).
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bioRxiv - Genetics 2024Quote: To determine the T-DNA insertion site of line ClvRubq7 we extracted genomic DNA from two different plants and constructed sequencing libraries using NEBNext® Ultra™ II FS DNA Library Prep Kit for Illumina (NEB #E7805) following manufacturer’s instructions ...
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bioRxiv - Genetics 2024Quote: ... and ligated with T4 DNA ligase (NEB M0202) into BsmbI site of vector backbones ...
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bioRxiv - Cell Biology 2024Quote: ... blunted using DNA polymerase I large Klenow fragment (New England Biolabs) and inserted into the lentiviral vector pWPI Puro carrying the puromycin resistance gene ...
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bioRxiv - Genetics 2024Quote: ... Cloning was performed by Gibson Assembly of PCR amplified or commercially synthesized gene fragments (from Integrated DNA Technologies or Twist Bioscience) using NEBuilder Hifi Master Mix (NEB Cat# E262), and final plasmids sequence-verified by Sanger sequencing of the open reading frame and/or commercial whole-plasmid sequencing service provided by Primordium.
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bioRxiv - Genetics 2024Quote: ... 10ul 10x NEB rCutSmart Buffer (NEB B6004S), 79.2ul nuclease-free water ...
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bioRxiv - Genetics 2024Quote: ... digested with Exonuclease-I (New England Biolabs) at 37 °C for 30 min ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and purified the rest of the digestion product with Monarch Nucleic Acid Purification Kit (NEB). We quantified the amount of purified DNA using the Qubit dsDNA HS Assay (Thermofisher ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and performed colony PCRs with Taq polymerase (NEB). We used the Monarch PCR and DNA Cleanup Kit (NEB ...
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bioRxiv - Cell Biology 2024Quote: ... sequences of all myc-his tagged ZFP36 proteins were digested using ClaI and PmeI restrictions enzymes (New England Biolabs), blunted using DNA polymerase I large Klenow fragment (New England Biolabs ...
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bioRxiv - Cell Biology 2024Quote: Prior to rRNA depletion using the NEBNext rRNA Depletion Kit v2 (New England Biolabs), 1 μg total RNA was mixed with 2 μl 1:100 diluted ERCC RNA Spike-In Mix (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2024Quote: ... Libraries were prepared with the NEBNext Ultra II Directional RNA Kit (NEB). Samples were equimolarly pooled and sequenced on a NextSeq550 system (Illumina ...
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bioRxiv - Cell Biology 2024Quote: ... Equimolar amounts of complementary oligonucleotides were phosphorylated by incubation with T4 polynucleotide kinase in T4 ligation buffer (New England Biolabs) for 30 min at 37°C ...
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bioRxiv - Cell Biology 2024Quote: ... using primers flanked by ClaI and XbaI restriction enzymes (New England Biolabs), digested and inserted into pcDNA3-TTP-mycHis vector backbone digested with the same restriction enzymes ...
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bioRxiv - Cell Biology 2024Quote: ... 100 µg cell lysate was mixed with 3,200 U λ protein phosphatase (New England Biolabs) and incubated at 37 °C for 1 h to achieve complete protein dephosphorylation ...
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bioRxiv - Cell Biology 2024Quote: ... The DNAse-digested RNA was purified using the Monarch RNA Cleanup kit (New England Biolabs) as recommended by the manufacturer and used as template for cDNA synthesis and SYBR Green-based qPCR (see quantitative real-time PCR (RT-qPCR ...
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bioRxiv - Genetics 2024Quote: ... Libraries were prepared using Ultra II RNA library kit (NEB) and samples were sequenced on the NovaSeq 6000 platform (Illumina ...
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bioRxiv - Evolutionary Biology 2024Quote: ... were transcribed and purified using HiScribe T7 High Yield Transcription Kit (New England BioLabs) and illustra Microspin G-50 Columns (GE Healthcare Life Sciences) ...
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bioRxiv - Cell Biology 2024Quote: ... and 0.2 U/μl RNase H (NEB #M0297) and immediately used for the overhang assay as follows ...
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bioRxiv - Developmental Biology 2024Quote: ... and RNA was extracted using the Monarch Total RNA Miniprep Kit (New England Biolabs). cDNA was made using iSCRIPT reverse transcriptase (BioRad) ...
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bioRxiv - Cell Biology 2024Quote: ... Linear templates were prepared by digesting CsCl density gradient purified DNA with AscI (NEB) (terminal repeats template ...
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bioRxiv - Cell Biology 2024Quote: ... Deproteinized reactions (63 μl) were incubated for 45 min at 37°C in 1X ThermoPol buffer (NEB) with 15 units E ...
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bioRxiv - Cell Biology 2024Quote: ... 1.56 μM annealed oligonucleotides and 12,000 units T4 Ligase (NEB). Ligation reactions were quenched by addition of EDTA to 25 mM and proteins were digested by incubation at 37°C for 20 min with 0.1% SDS and 20 units/ml proteinase K (NEB) ...
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bioRxiv - Developmental Biology 2024Quote: ... then capped with m7G (5’) ppp (5’) G (NEB) and tailed with a poly(A ...
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bioRxiv - Genetics 2024Quote: ... using fragments synthesized by Q5 High Fidelity Taq polymerase (New England Biolabs). Finally ...
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bioRxiv - Cell Biology 2024Quote: ... Reactions were quenched by addition of EDTA to 50 mM and proteins were removed by treatment with proteinase K (20 units/ml) (NEB) and SDS (0.25% ...
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bioRxiv - Cell Biology 2024Quote: ... 1.25 μM annealed oligonucleotides and 12,000 units T4 Ligase (NEB). For the no-overhang template ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were removed by treatment with proteinase K (20 units/ml) (NEB) and SDS (0.25% ...
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bioRxiv - Cell Biology 2024Quote: ... or PciI (NEB) (internal repeats template) ...
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bioRxiv - Cell Biology 2024Quote: ... Digest were quenched by addition of EDTA to 25 mM and proteins were digested by incubation at 37°C for 40 min following addition of SDS and Proteinase K (NEB) to 0.25% and 20 units/ml respectively ...
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bioRxiv - Cell Biology 2024Quote: ... Ligation reactions were quenched by addition of EDTA to 25 mM and proteins were digested by incubation at 37°C for 20 min with 0.1% SDS and 20 units/ml proteinase K (NEB). Unligated oligonucleotides were removed by gel filtration through a 50 cm x 0.7 cm Sepharose 4B column (Sigma ...