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Citations for New England Biolabs :
401 - 450 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... the concentrated protein at 3 mg/mL was incubated with c-AMP Protein Kinase A PKA (NEB) (molar ratio of 40:1 ...
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bioRxiv - Biochemistry 2024Quote: ... and 1 mM DNA oligo in T4 polynucleotide kinase buffer (NEB). This mixture was then incubated at 37°C for 1 hour ...
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bioRxiv - Biochemistry 2024Quote: ... The ATP and ADP makers were created using T4 polynucleotide kinase (NEB). T4 PNK mixed with 128 µM ATP supplemented with 10 nM [α-32 P]-ATP ...
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bioRxiv - Biochemistry 2024Quote: ... and synthesized using the HiScribe™ T7 High Yield RNA Synthesis Kit (NEB, E2040S), following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... Dephosphorylation reactions were performed with Lambda Protein Phosphatase (Lambda PP) (NEB). 1,000 units of Lambda PP were utilized per µmol of Ycf1 protein in a 500 µL reaction ...
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bioRxiv - Biochemistry 2024Quote: ... coli (NEB). Affinity purification of Strep3-tagged proteins was performed using a Strep-Tactin® Superflow® high capacity FPLC column (IBA Lifesciences).
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bioRxiv - Biochemistry 2024Quote: ... C-terminal Cx36 mutants were generated using the Q5 site directed mutagenesis kit (E0554S; New England Biolabs, Ipswitch, MA) and corresponding primer pairs introducing gaps into the Cx36 coding sequence ...
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bioRxiv - Biochemistry 2024Quote: Velaglucerase (VRPIV®, Takeda) and Imiglucerase (Cerezyme®, Sanofi) were deglycosylated using PNGase F enzyme (New England Biolabs). The deglycosylation reaction was performed according to the recommendation from the manufacturer ...
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bioRxiv - Biochemistry 2024Quote: ... coli (NEB, C2527H) via autoinduction ...
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bioRxiv - Biochemistry 2024Quote: Purified MBP-fusion proteins were treated with TEV-protease (NEB, P8112S) for 1 hour at room temperature ...
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bioRxiv - Biochemistry 2024Quote: ... Gene fragments were subcloned into a pMAL-c6T (NEB, N0378S) expression vector by Gibson Assembly.
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bioRxiv - Biochemistry 2024Quote: ... and purified on amylose resin (NEB, E8021S) according to the manufacturer’s instructions.
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bioRxiv - Biochemistry 2024Quote: Constructs bearing a C-terminal cMyc tag were cloned by site-directed mutagenesis (NEB, M0554S) using oligonucleotides as primers bearing a 5’-overhang encoding the inserted sequence ...
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bioRxiv - Biochemistry 2024Quote: ... coli T7Express cells (New England Biolabs) cells transformed with pET28a expression vector where the gene for the C-terminal His6-tagged Synechococcus elongatus NusG was cloned ...
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bioRxiv - Biochemistry 2024Quote: ... A 13 nt RNA oligonucleotide was radiolabeled at the 5’ end with [γ-32P] ATP and T4 polynucleotide kinase (New England Biolabs) prior to EC assembly ...
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bioRxiv - Biochemistry 2024Quote: ... and NEB-Gibson Assembly (New England Biolabs #E2611S). All point mutations in PLD3 and PLD4 were generated using Q5-site directed mutagenesis kit (New England Biolabs #E0554S).
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bioRxiv - Biochemistry 2024Quote: ... The inserts were each ligated with digested pmirGLO plasmids using a quick ligasation kit (New England Biolabs) and transformed into JM109 competent cells (Promega) ...
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bioRxiv - Biochemistry 2024Quote: ... All point mutations in PLD3 and PLD4 were generated using Q5-site directed mutagenesis kit (New England Biolabs #E0554S).
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bioRxiv - Biochemistry 2024Quote: ... The plasmid was verified by digestion with the NotI restriction enzyme (New England Biolabs), according to manual.
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bioRxiv - Biochemistry 2024Quote: ... Eluted proteins were loaded onto a 6 mL amylose column (New England Biolabs), washed in 50 mM HEPES-KOH (pH 7.5) ...
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bioRxiv - Biochemistry 2024Quote: ... The insert was digested by XhoI and BglII (New England Biolabs) and ligated with digested pCMV vector ...
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bioRxiv - Biochemistry 2024Quote: ... 600 u micrococcal nuclease (NEB, M0247S) and incubation at 37°C for 2 min with 1,250 rpm shaking ...
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bioRxiv - Biochemistry 2024Quote: ... Bound proteins were eluted by digestion with 500 units micrococcal nuclease (New England Biolabs) for 10 minutes at 37°C in 25 mM HEPES-KOH (pH 7.6) ...
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bioRxiv - Biochemistry 2024Quote: ... 150 μM S-adenosyl-methionine (NEB)) ...
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bioRxiv - Biochemistry 2024Quote: ... Fractions containing Cdt1 were further purified on a 5 mL amylose column (New England Biolabs) in 50 mM Tris-HCl (pH 7.8) ...
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bioRxiv - Biochemistry 2024Quote: ... Peak fractions were further purified on 7.5-10 mL amylose columns (New England Biolabs) in 50 mM Tris-HCl (pH 7.8) ...
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bioRxiv - Biochemistry 2024Quote: ... The protein was further purified on a 5-10 mL amylose column (New England Biolabs) in 50 mM Tris-HCl (pH 7.8) ...
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bioRxiv - Biochemistry 2024Quote: ... The protein was expressed using the strain T7express lysY (NEB, C3010I). Expression was induced with 0.4 mM IPTG at OD600 ∼0.6 at 16°C ...
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bioRxiv - Biochemistry 2024Quote: ... crude extracts were treated with the λ-protein phosphatase (λ-PPase; New England BioLabs, Cat# P0753S) before application on the gel ...
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bioRxiv - Biochemistry 2024Quote: ... the crude extract containing 100 μg (in 100 μL volume) of proteins was treated with 400 units of λ-PPase (#P0753; New England Biolabs) for 60 min at 30 °C ...
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bioRxiv - Biochemistry 2024Quote: ... coli BL21 (DE3) cells (New England BioLabs).
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bioRxiv - Biochemistry 2024Quote: ... 5’-end radiolabeled DNA was generated with T4 polynucleotide kinase (M0201L, NEB) and [γ-32P]ATP (NEG035C005MC ...
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bioRxiv - Biochemistry 2024Quote: ... and 0.5 μL of ExoI (NEB) was mixed into each reaction ...
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bioRxiv - Biochemistry 2024Quote: ... and the supernatant was digested for 1 h at 37°C with PNGase F (New England Biolabs, P0704) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... Two-hundred and fifty ng of RNA were used for reverse transcription reactions using the LunaScript RT SuperMix (New England BioLabs cat# E3010). For the qPCR ...
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bioRxiv - Bioengineering 2024Quote: ... coli DH5α (Matthew Scott Lab, University of Waterloo) and NEB10-β (New England Biolabs (NEB), Whitby ...
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bioRxiv - Bioengineering 2024Quote: ... comprising 15 μL of Phusion® High-Fidelity PCR Master Mix (New England Biolabs, Ipswich, MA), 0.2 μM of forward and reverse primers ...
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bioRxiv - Bioengineering 2024Quote: ... PCR products were assembled using NEB Hifi DNA Assembly Master Mix (NEB) according to the manufacturer instructions ...
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bioRxiv - Bioengineering 2024Quote: ... The pTA-Mob plasmid (gift from Thomas Hamilton and David Edgell, Western University) was linearized using AvrII (NEB) and combined with the amplified fragment from pFAST-PETase-cis using a modified NEB Hifi Assembly protocol with a two-hour assembly step at 50 °C ...
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bioRxiv - Bioengineering 2024Quote: ... or Q5 Hot Start High-Fidelity 2x Mastermix (M0492, NEB) in a 15 μL reaction ...
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bioRxiv - Bioengineering 2024Quote: ... Amplicons from mouse embryo lysates were generated using Q5 Hot Start High-Fidelity 2x Mastermix (M0492, NEB) in a 25 μL reaction ...
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bioRxiv - Bioengineering 2024Quote: ... And cloned into a expression plasmid under the control of the EF1α using NEB High-Fidelity DNA Assembly 2x Master Mix (New England Biolabs). To generate 3’ and 5’ stop codon reporters ...
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bioRxiv - Bioengineering 2024Quote: ... either half of the EGFP open reading frame was amplified from pLHA-TR-EF1α-Hipk3-Polio-SplitGFP45 with primers 49 and 50 (first half of EGFP ORF) and primers 51 and 52 (second half of EGFP ORF) using Q5® High-Fidelity DNA Polymerase (New England Biolabs) for 35-cycles ...
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bioRxiv - Bioengineering 2024Quote: All polymerase chain reactions were performed using Q5 High-Fidelity 2X Master Mix (New England Biolabs) and all primers were synthesized by IDT (Integrated DNA Technologies) ...
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bioRxiv - Bioengineering 2024Quote: LunaScript Primer-Free RT Master Mix Kit (NEB E3025S) was used to generate cDNA using primers oAS344 for cRNA and oAS345 for vRNA (Supplementary Note 21).
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bioRxiv - Bioengineering 2024Quote: ... The mara1 fragment was cloned into pRM4e vector using NEBuilder HiFi DNA Assembly (New England Biolabs) master mix and introduced into E ...
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bioRxiv - Bioengineering 2024Quote: ... digesting with DpnI (NEB R0176L), and assembling by isothermal assembly ...
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bioRxiv - Bioengineering 2024Quote: ... qPCR reactions were set up using the Luna Universal qPCR Master Mix (NEB M3003S). Reactions included 6 µL of Luna Mastermix ...
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bioRxiv - Bioengineering 2024Quote: Q5 DNA Polymerase was used for DNA amplification and colony PCR screening (New England Biolabs, Germany). Gibson Assembly and site-directed mutagenesis was performed using the NEBuilder HiFi DNA Assembly Cloning Kit (E5520S ...
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bioRxiv - Bioengineering 2024Quote: ... This fragment was cloned into a lentiviral expression plasmid we have previously described (31) encoding the Notch1 core linked to the tTA transcription factor using NEBuilder HiFi DNA Assembly (New England Biolabs). The resultant plasmid encoded constitutive expression of CII-synNotch receptor from an EF1α promoter and production of the puromycin N-acetyl-transferase transgene via the constitutive PGK promoter for antibiotic selection ...