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Citations for New England Biolabs :
401 - 450 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2024Quote: ... libraries were quantified in triplicate using NEB Luna® Universal Probe qPCR Master Mix (New England Biolabs, Ipswitch, MA), Illumina PhiX standard (San Diego ...
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bioRxiv - Genomics 2024Quote: ... and the supernatant was used for following steps: The cleaned DNA was treated with thermolabile ExoI and ExoVII (NEB) to remove primers (37°C x 30 min ...
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bioRxiv - Genomics 2024Quote: ... 0.5µL Q5 Taq (NEB), 0.2µM dNTP ...
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bioRxiv - Genomics 2024Quote: ... libraries were prepared in quadruplicate from 1ug DNA using the NEBNext® Ultra II ligation kit (New England Biolabs, Ipswitch, MA) according to the manufacturer’s protocols ...
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bioRxiv - Genomics 2024Quote: ... 1X Q5 buffer (NEB), 0.5µL Q5 Taq (NEB) ...
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bioRxiv - Genomics 2024Quote: Two microliters of the cDNA were used for PCR amplification employing Taq polymerase (Genaxxon Bioscience) for HEK293T-derived samples and Q5® High-Fidelity DNA Polymerase (New England Biolabs). Primers are listed in Supplementary Table 5 ...
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bioRxiv - Genomics 2024Quote: ... after which the RNA probes were purified using the Monarch® RNA Cleanup kit (50 µg) (New England Biolabs, Ipswitch, MA). Finally ...
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bioRxiv - Genomics 2024Quote: ... For this HiScribe® T7 High Yield RNA Synthesis Kit (New England Biolabs, Ipswitch, MA) was used according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2024Quote: ... and 0.5 units of Phusion Polymerase (NEB) (total volume ...
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bioRxiv - Evolutionary Biology 2024Quote: ... we generated libraries using the NEBNext Ultra II protocol for Illumina (NEB, E7645S-E7103S), with minor adjustments ...
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bioRxiv - Genomics 2024Quote: ... 4 μg of the barcoded plasmid library was linearized by digestion with NruI-HF (NEB #R3192) at 37°C for 16 hours ...
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bioRxiv - Genomics 2024Quote: ... gDNA was first amplified with primers JLSPr141–144 and JLSPr165–168+171+172 using Q5 High-Fidelity DNA Polymerase (NEB #M0491) for 20 cycles with an annealing temperature of 65°C ...
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bioRxiv - Genomics 2024Quote: ... 1 μg of the step one plasmid library was linearized with XhoI (NEB #R0146) at 37°C for 16 hours ...
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bioRxiv - Genomics 2024Quote: ... was first generated by removing BsmBI restriction sites on pU6-sgRNA-EF1Alpha-puro-T2A-BFP (Addgene #60955)35 through Golden Gate Assembly (NEB E1602S). Then ...
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bioRxiv - Genomics 2024Quote: Each of the fragmented protein factor-enriched chromatin sample was mixed with 50 µg/ml of BSA (cat# B9000S, NEB) to prevent chromatin aggregation ...
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bioRxiv - Genomics 2024Quote: ... the input concentration per pool varied (i.e., for clinical samples using iTru/NEB, the input was between 25-80 ng/µL ...
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bioRxiv - Genomics 2024Quote: ... the intermediate library was digested with Esp3I enzyme (NEB R0734S) at 37°C for 6 hr and gel purified ...
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bioRxiv - Genetics 2024Quote: ... polyA+ mRNA was isolated from total RNA with NEBNext Poly(A) mRNA Magnetics Isolation Module (New England Biolabs). RNA-Seq libraries were prepared with NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England Biolabs) ...
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bioRxiv - Genetics 2024Quote: ... using BbsI (NEB) restriction sites ...
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bioRxiv - Genetics 2024Quote: ... and BstZ17l (NEB) digest was extracted from an agarose gel and co-transfected with the pX330 plasmid into the Htr5 cell line using the PEI transfection method ...
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bioRxiv - Genetics 2024Quote: ... Broad Range (NEB) was used as a molecular weight marker ...
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bioRxiv - Genetics 2024Quote: ... A fragment produced by KpnI (NEB) and BstZ17l (NEB ...
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bioRxiv - Genetics 2024Quote: ... 400 nM of each of the flanking primers PS1057-NGS-F and PS1057-NGS-R (S2 File) and 0.1 units/µl of LongAmp Taq DNA polymerase (NEB Cat. # M0323L). In a second amplification step ...
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bioRxiv - Genetics 2024Quote: ... and 3 units of Taq DNA Polymerase (New England Biolabs, Inc.) under the following reaction conditions ...
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bioRxiv - Genetics 2024Quote: ... 1x Standard Taq Buffer (New England Biolabs, Inc.), 200µM each deoxynucleotide triphosphates ...
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bioRxiv - Genetics 2024Quote: ... The B splice form (Spc105RB) was generated using site directed mutagenesis to delete the first intron from the A form (NEB BaseChanger Kit). All mutants were generated using site specific mutagenesis of the A- or B-form Spc105R coding region in the pENTR4 vector ...
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bioRxiv - Genetics 2024Quote: ... The genes for the probes were amplified using LongAmp Taq 2X Master Mix (New England Biolabs) with optimized PCR cycling parameters ...
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bioRxiv - Immunology 2024Quote: ... meningosepticum) and Endo F3 (NEB) (designated as F1 ...
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bioRxiv - Immunology 2024Quote: ... 5 μL of GC Enhancer (NEB), 5 μL of 5X buffer,10 mM dNTPs ...
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bioRxiv - Immunology 2024Quote: ... TAP-PCR reaction was performed using 5 μL of Q5 polymerase (NEB), 5 μL of GC Enhancer (NEB) ...
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bioRxiv - Immunology 2024Quote: ... cDNA synthesis was performed using ProtoScript M-MuLV First Strand cDNA synthesis kit (New England Biolabs) and random hexamer primers ...
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bioRxiv - Immunology 2024Quote: ... Libraries were prepared using the NEBNext Ultra II DNA library prep kit for Illumina (New England Biolabs) according to manufacturer’s instructions with the following specifications and modifications ...
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bioRxiv - Immunology 2024Quote: ... top and bottom strand oligos (1 µl of 100 µM each) were phosphorylated with T4 PNK (New England Biolabs) for 30 minutes at 37 °C ...
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bioRxiv - Immunology 2024Quote: ... NEBNext adaptor for Illumina from NEBNext Multiplex Oligos for Illumina (New England Biolabs) was diluted 25-fold in TBS buffer ...
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bioRxiv - Genomics 2024Quote: ... Sequencing libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit (New England Biolabs) for Illumina according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... Second-strand synthesis was performed by addition of 2 µl Klenow-Fragment DNA Polymerase (New England Biolabs) and incubation for 15 minutes at 30°C ...
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bioRxiv - Immunology 2024Quote: ... including DNAseI (RNase free, New England Biolabs) digestion to remove genomic DNA ...
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bioRxiv - Immunology 2024Quote: Reverse-transcription on total RNA (100 to 500 ng) was performed with random hexamer/oligo-dT-Primer containing LunaScript RT Supermix (New England Biolabs) following the manufactureŕs instructions ...
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bioRxiv - Immunology 2024Quote: ... Q5 NEBNext® Ultra™ II Q5® Master Mix (New England Biolabs) for 10-14 cycles ...
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bioRxiv - Immunology 2024Quote: ... 0.15 µl Murine RNase Inhibitor (New England Biolabs) and 0.2 µl Superscript III enzyme (Thermofisher ...
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bioRxiv - Immunology 2024Quote: ... 1 µl per 50 µl pool volume of Thermolabile Exonuclease I (New England Biolabs) was then added and incubated for 4 minutes at 37 °C ...
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bioRxiv - Immunology 2024Quote: ... Two microliters were subsequently used in real-time quantitative PCR reactions containing SYBR-green based Luna universal dye qPCR mix (New England Biolabs) and gene specific PCR primers ...
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bioRxiv - Genomics 2024Quote: ... The cDNA was then used as input for the first-round PCR with oJL105 and oJL106 to add the i5/i7 primer sites using Q5 DNA polymerase (NEB M0491L). Libraries were amplified with an initial 98 °C denaturation step for 30 s ...
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bioRxiv - Genomics 2024Quote: ... Reverse transcription was performed with dT priming using the ProtoScript II RT (NEB M0368L) as described by the manufacturer ...
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bioRxiv - Genomics 2024Quote: ... and half the elution was used as a template in a second round of 8-10 cycle PCR to prepare Illumina libraries using NEBNext multiplex oligos using the same conditions described above (NEB E7335L and E7500L). The final PCR product was purified using AMPure XP beads (Beckman Coulter A63882 ...
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bioRxiv - Genomics 2024Quote: ... and 2 U/µl Thermolabile Exonuclease I (New England Biolabs, catalog no. M0568S) and incubated for 15 min at 37 °C on a shaking thermoblock at 400 rpm ...
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bioRxiv - Genomics 2024Quote: ... samples containing a mixture of single-cell ATAC and RNA libraries were resuspended in the pre-amplification mastermix containing 1x NEBNext HF 2x PCR Master Mix (NEB, catalog no. M0541L) and a combination of three primers ...
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bioRxiv - Microbiology 2024Quote: ... Plasmids containing either rpsL-cat or the inducible promoter tetO1 [18] flanked by regions homologous to the DNA up- and downstream of the ggt-promoter were constructed using a commercially available Gibson Assembly Cloning Kit (NEB). Homologous regions were amplified from the genomic DNA of strain PMSS1 using primer pairs FlgK fwd/FlgK rev (rpsL/tetO1 ...
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bioRxiv - Microbiology 2024Quote: ... All three fragments were purified and combined in a Gibson assembly (New England Biolabs [41]). Sequencing revealed a point mutation in the cd3284 open reading frame ...
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bioRxiv - Microbiology 2024Quote: ... Single-construct plasmids expressing A-subunits were constructed via restriction digestion (NdeI and PstI, New England Biolabs) and ligation using pBAD24 (Amp+ ...