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Citations for New England Biolabs :
301 - 350 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2024Quote: ... IVT-produced RNA samples were purified using a Monarch® RNA Cleanup Kit (New England Biolabs). ssRNA during RNA extractions was degraded with RNase T1 (Thermo Scientific) ...
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bioRxiv - Synthetic Biology 2024Quote: ... Ligations were performed using Quick Ligation™ Kit (New England Biolabs). HPLC and LC-MS mobile phases were prepared using ≥99.0% (GC ...
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bioRxiv - Synthetic Biology 2024Quote: ... and purified using Monarch® PCR & DNA Cleanup Kit (New England Biolabs). Gel fragments were extracted using GeneJet Gel extraction kit (Thermo Fisher Scientific) ...
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bioRxiv - Synthetic Biology 2024Quote: ... All constructs were assembled into a novel lentiviral destination backbone50,55 via a PaqCI reaction following manufacturer instructions (New England Biolabs #R0745S). The Gal4-NGN2 plasmid was constructed using Gibson Assembly with In-Fusion® Snap Assembly Master Mix (Takara Bio #638947 ...
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bioRxiv - Synthetic Biology 2024Quote: ... In vitro transcription (IVT) eactions were performed with HiScribe™ T7 High Yield RNA Synthesis Kit (New England Biolabs). Template DNA was degraded with TURBO DNase (Thermo Fisher Scientific) ...
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bioRxiv - Synthetic Biology 2024Quote: All replicative plasmid backbones were constructed using the Golden Gate Assembly strategy with the type II restriction enzyme Esp3I (NEB) according to the manufacturer’s protocol (Table 1) ...
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bioRxiv - Synthetic Biology 2024Quote: ... Amplicons are treated with 15U of Dpn1 (NEB) for 2.5 hours at 37°C followed by 20 minutes at 80°C ...
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bioRxiv - Synthetic Biology 2024Quote: ... DH10B (NEB) electrocompetent cells are transformed with 2μL of diluted isothermal assembly reaction ...
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bioRxiv - Synthetic Biology 2024Quote: ... Isothermal assembly followed Gibson Assembly protocols (NEB), but contained 100 mM Tris-HCl pH 7.5 ...
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bioRxiv - Synthetic Biology 2024Quote: ... and backbone were assembled in a single Golden Gate reaction (NEB) as described above.
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bioRxiv - Synthetic Biology 2024Quote: ... and TtgR_ColE1_SPS_V5 backbone were assembled using Golden Gate assembly (NEB). The libraries with approximately 15 barcodes per variant ...
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bioRxiv - Synthetic Biology 2024Quote: ... using the NEBNext Ultra II Q5 Master Mix (NEB, #M0544), primers (0.5 μM each ...
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bioRxiv - Synthetic Biology 2024Quote: ... The spacer was inserted into the backbone using Golden Gate (NEB) following standard protocols.
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bioRxiv - Synthetic Biology 2024Quote: ... 1ng of RNA was added to Luna Universal One-Step qRT-PCR mix (NEB) containing 4μmol of each primer on ice ...
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bioRxiv - Synthetic Biology 2024Quote: ... which were performed using OneTaq® Polymerase (NEB). For plasmid assembly ...
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bioRxiv - Synthetic Biology 2024Quote: Plasmid libraries are generated using Golden Gate Assembly Kits (NEB, BsaI-HFv2). The reactions undergo a cycling protocol of 30 alternating 5-minute 37°C and 16°C cycles followed by a final 60°C 5-minute hold ...
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bioRxiv - Synthetic Biology 2024Quote: ... The purified RNA was digested using 4U DnaseI (NEB) in a 50μL reaction incubated at 37°C for 30 minutes ...
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bioRxiv - Synthetic Biology 2024Quote: ... along with 8 units of RNAse inhibitor (NEB), and 10 µm malachite green oxalate added to each reaction ...
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bioRxiv - Synthetic Biology 2024Quote: ... Purified plasmids were first amplified and linearized using Q5 Hot Start High-Fidelity DNA Polymerase (NEB); forward and reverse primers are listed in Table S5 ...
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bioRxiv - Synthetic Biology 2024Quote: Plasmid cloning was performed primarily using standard PCR and restriction enzyme cloning with Phusion DNA Polymerase (NEB #M0530L), restriction enzymes (NEB) ...
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bioRxiv - Synthetic Biology 2024Quote: ... 1 μL of diluted GA reaction was then used in a 50 μL PCR reaction using Q5 Hot Start High-Fidelity DNA Polymerase (New England Biolabs).
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bioRxiv - Neuroscience 2024Quote: ... The CROP-seq vector backbone (Datlinger et al., 2017) was obtained from addgene (addgene: #86708) and digested using New England Biolabs’ (NEB) BsmBI enzyme ...
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bioRxiv - Molecular Biology 2024Quote: ... Libraries were prepared with NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB, E7645) and analyzed with either 2100 Bioanalyzer or TapeStation systems (Agilent) ...
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bioRxiv - Molecular Biology 2024Quote: dsRNA was generated by in vitro transcription (NEB T7 E2050s transcription kit) from PCR products generated by the following forward and reverse primers (separated by comma):
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bioRxiv - Neuroscience 2024Quote: ... using fragments obtained from PCR with Q5 or Phusion (NEB) or Platinum SuperFi DNA Polymerase (Invitrogen) ...
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bioRxiv - Neuroscience 2024Quote: Molecular cloning was performed using NEB HiFi Assembly (New England BioLabs (NEB)) using fragments obtained from PCR with Q5 or Phusion (NEB ...
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bioRxiv - Neuroscience 2024Quote: Molecular cloning was performed using NEB HiFi Assembly (New England BioLabs (NEB)) using fragments obtained from PCR with Q5 or Phusion (NEB ...
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bioRxiv - Biochemistry 2024Quote: ... After restriction with the corresponding restriction enzymes (New England Biolabs, Ipswich, MA, USA) (Table S1 ...
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bioRxiv - Biochemistry 2024Quote: ... the membrane was incubated with HRP-conjugated secondary antibody (goat anti-rabbit HRP, BioLabs 7074P2) in 3% BSA in PBS-T shaking for 1-2 h at room temperature ...
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bioRxiv - Biochemistry 2024Quote: ... Protease Inhibitors) with recombinant PKA kinase (2500 U/mg, NEB-P600S) and 1 mM ATP overnight at 30°C and 250 rpm ...
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bioRxiv - Microbiology 2024Quote: ... Plasmid DNA with similar profiles was additionally differentiated by digestion with the BamHI and HindIII restriction enzymes (NEB, USA). The digestion was performed according to the manufacturer’s recommendation ...
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bioRxiv - Molecular Biology 2024Quote: ... Conditional gene deletion was assessed by PCR amplification of genomic DNA using Taq polymerase (NEB) and oligonucleotides SMD357/8 flanking the TbGT8 locus ...
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bioRxiv - Immunology 2024Quote: ... and ligated (Quick Ligase, NEB) into a modified pENTR4-LP55 vector containing the monomeric Fc (“monoFC” ...
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bioRxiv - Molecular Biology 2024Quote: ... the plug was incubated in 160 μL of 1× NEBuffer 3.1 buffer containing 160 units of Bgl II (New England Biolabs) overnight at 37°C ...
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bioRxiv - Molecular Biology 2024Quote: ... Six microliters of 6x Gel Loading Dye (B7025S, New England Biolabs) and 1.5 μL of 2.5 mg/ml EtBr were added to each sample ...
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bioRxiv - Molecular Biology 2024Quote: ... and Phusion® High-Fidelity DNA Polymerase (New England Biolabs, USA). PCR products were purified on a 1% agarose gel and extracted using the GeneJET G el Extraction Kit (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... using the NEBNext Poly(A) mRNA Magnetic Isolation Module and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs (NEB), Ipswich ...
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bioRxiv - Cell Biology 2024Quote: ... was performed to amplify the DNA sequence around the knockin position using Phusion high-fidelity DNA polymerase master mixture reaction (NEB); forward primer (5’-CTT CGG AAT GAG TCT CTG TCA TC-3’ ...
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bioRxiv - Neuroscience 2024Quote: ... using the NEBNext Poly(A) mRNA Magnetic Isolation Module and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs (NEB), Ipswich ...
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bioRxiv - Cell Biology 2024Quote: ... The 13 mutant LEP sequences containing specific point mutations were generated from the original sequence by carrying out PCRs with mutagenic primers and using a KLD enzyme mix (M0554S; NEB) to repair the vectors ...
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bioRxiv - Cell Biology 2024Quote: ... and 0.4 U/μL Terminal Deoxynucleotidyl Transferase (NEB) were mixed in 1× Terminal Transferase Reaction buffer and incubated at 37°C overnight ...
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bioRxiv - Cell Biology 2024Quote: crRNAs were synthesized using HiScribe T7 Quick High Yield RNA Synthesis Kit (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... cDNA synthesis was achieved with the NEBNext RNA First Strand Synthesis and NEBNext Ultra Directional RNA Second Strand Synthesis Modules (New England BioLabs). The remaining steps of library preparation were done using and the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England BioLabs) ...
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bioRxiv - Cell Biology 2024Quote: ... The remaining steps of library preparation were done using and the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England BioLabs). Adapters and PCR primers were purchased from New England BioLabs ...
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bioRxiv - Cell Biology 2024Quote: ... and PCR was performed using Q5® High-Fidelity 2X Master Mix (NEB). The splicing efficiency is monitored using RT-PCR with primers located on two adjacent exons ...
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bioRxiv - Cell Biology 2024Quote: ... The 3′ cDNA adapter was ligated by T4 RNA ligase 1 (NEB) by incubating at 25 °C for 16h ...
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bioRxiv - Molecular Biology 2024Quote: ... The resulting products were pre-amplified in a 100 μL reaction using 10 cycles with 4 μL pre-amp primers (10x Genomics PN 20002714) and 50 μL of NEBNext High-Fidelity 2X PCR Master Mix (NEB, M0541S). Reactions were cleaned with 1.6X SPRI and eluted in 40 μL EB ...
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bioRxiv - Molecular Biology 2024Quote: ... 50 μL NEBNext High-Fidelity 2X PCR Master Mix (NEB, M0541S), 36.9 μL nuclease-free water and 2.5 μL of 10 μM sample index N (10x Genomics ...
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bioRxiv - Neuroscience 2024Quote: ... followed by deoxyribonuclease I (NEB) treatment and subsequently purified using Agencourt RNAClean XP magnetic beads (Beckman Coulter) ...
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bioRxiv - Neuroscience 2024Quote: ... The amplified PCR product was then digested by restriction enzymes of AgeI (New England Biolabs, #R0552S) and MfeI (New England Biolabs ...