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Citations for New England Biolabs :
301 - 350 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2024Quote: ... electroporated into 10beta electrocompetent cells (NEB), then the 1-hour-recovered cells were plated to select on LB+Carb plates ...
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bioRxiv - Zoology 2024Quote: ... For fresh specimens a shearing step with NEBNext dsDNA Fragmentase (New England Biolabs) was performed before library preparation ...
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bioRxiv - Systems Biology 2024Quote: Each tagged-ORF plasmid subpool was treated with I-SceI (NEB) in the provided buffer (at a volume of 50uL for ∼1ug hORF plasmids and 200uL for ∼4ug vORF plasmids) ...
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bioRxiv - Systems Biology 2024Quote: Approximately 6ng of each loxcode cassette was combined with approximately 75ng of the linear digested plasmid pool in a final 10uL of 1x HiFi assembly reaction (NEB). This was incubated on a thermocycler at 50C for 15min ...
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bioRxiv - Microbiology 2024Quote: ... and Luna Probe One-Step RT-qPCR 4X Mix with UDG (NEB) according to the manufacturer’s recommendations ...
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bioRxiv - Developmental Biology 2024Quote: ... the AT1G65620/AS2 coding sequence (600 bps) was amplified from Col-0 cDNA using primers oLH111 and oLH112 by Phusion PCR (NEB), and cloned into pENTR™/D-TOPO™ (Invitrogen™) ...
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bioRxiv - Systems Biology 2024Quote: The dsDNA loxcode cassettes were formed by combining two purchased oligos (IDT) in a 50uL Q5 (NEB) polymerase reaction with the Q5 polymerase ...
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bioRxiv - Systems Biology 2024Quote: ... This was cooled and treated with phosphatase rSAP (NEB) at 37C for 30min ...
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bioRxiv - Systems Biology 2024Quote: ... The poly(A) mRNA-enriched libraries were constructed using NEBNext® Ultra™ II RNA Library Prep Kit (New England BioLabs, Ipswich, MA). Paired-end sequencing (2×150 bp ...
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bioRxiv - Systems Biology 2024Quote: ... Ring-ligation was carried out using T4 DNA ligase (NEB) at 16 °C overnight ...
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bioRxiv - Systems Biology 2024Quote: ... Destination vectors were linearized using single-cutter restriction enzymes (New England Biolabs R0520L ...
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bioRxiv - Systems Biology 2024Quote: ... followed by purification using a Monarch PCR&DNA Cleanup Kit (NEB). Ring-ligation was carried out using T4 DNA ligase (NEB ...
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bioRxiv - Systems Biology 2024Quote: ... The samples then underwent two separate digestion reactions (with up to 4 µg of genomic DNA) using NlaIII and MseI enzymes (NEB) at 37°C overnight ...
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bioRxiv - Systems Biology 2024Quote: ... 1000 bp directly upstream and downstream of the HIS4-targeting sgRNA on the genome was PCR amplified and cloned on the upstream and downstream of the pENO1-Cas9-PptefT on D-227 vector using New England BioLabs (NEB) NEBuilder® HiFi DNA Assembly Master Mix ...
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bioRxiv - Plant Biology 2024Quote: ... qPCR was performed using the Luna Universal qPCR Master Mix (New England Biolabs, Ipswich MA) and the ThermoFisher StepOnePlus Real-Time PCR system to quantify the expression of GTL1 and the leaf proliferation stage marker ANGUSTIFOLIA 3 (AN3) ...
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bioRxiv - Plant Biology 2024Quote: ... and the supernatant was mixed and incubated with chitin beads (New England Biolabs). The PSM polypeptides were eluted with 50 mM DTT to cleave the intein ...
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bioRxiv - Plant Biology 2024Quote: ... Libraries were constructed using Ultra II DNA kit (NEB).
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bioRxiv - Plant Biology 2024Quote: cDNA libraries with different insert sizes were constructed using the NEB Next Ultra TM Directional RNA Library Prep Kit for Illumina (NEB, USA) following the manufacturer’s recommendations and were assessed on an Agilent Bioanalyzer 2100 system ...
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bioRxiv - Plant Biology 2024Quote: ... and ligated overnight at 16°C (T4 DNA ligase; NEB). The same approach was used for the second deletion (353 bp ...
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bioRxiv - Plant Biology 2024Quote: ... RNA-Seq libraries were prepared by NebNext Ultra II RNA library preparation kit (New England Biolabs, Massachusetts, USA) and sequenced using Illumina’s state-of-the-art NovaSeq 6000 V1.5 platform (Illumina ...
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bioRxiv - Plant Biology 2024Quote: ... was used for Level 1 and 3 assembly and SapI (New England BioLabs, USA) for Level 2 assembly ...
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bioRxiv - Plant Biology 2024Quote: ... Sequencing libraries were prepared using an NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs Japan, Tokyo, Japan) following the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2024Quote: ... The target loci in ERF74 and GAI genes were PCR amplified using Phusion High-Fidelity DNA polymerase (New England Biolabs) and gene-specific primers (Supplementary Table S1) ...
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bioRxiv - Plant Biology 2024Quote: ... when OD600 reached 0.5 for 4 h at 37 °C and then they were purified using amylose resin column (New England Biolabs, for MBP related proteins purification) or Ni-NTA agarose column (Qiagen ...
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bioRxiv - Plant Biology 2024Quote: ... polyA-enriched RNASeq libraries were prepared using a PolyA selection NEBNext Ultra II RNA kit (New England BioLabs, Ipswich MA) and sequenced on the Illumina NovaSeq S4 sequencing platform (2×150 bp reads ...
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bioRxiv - Plant Biology 2024Quote: ... the Luna® Universal qPCR Master Mix (New England Biolabs) was used ...
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bioRxiv - Plant Biology 2024Quote: ... cDNA was synthesized from 1 µg total RNA using the ProtoScript® II First Strand cDNA Synthesis Kit (NEB) with d(T)23VN primer.
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bioRxiv - Plant Biology 2024Quote: ... PCRs were performed with the Q5® High Fidelity DNA Polymerase (NEB) and specific ...
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bioRxiv - Biophysics 2024Quote: ... cleaved with BseRI using Gibson Assembly® Master Mix (NEB #E2611L) using the primers indicated in Table S1.
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bioRxiv - Plant Biology 2024Quote: ... Libraries were prepared using NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2024Quote: ... 0.2 mg/ml BSA (NEB), 1mM DTT.
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bioRxiv - Biophysics 2024Quote: ... and Proteinase K (15ug, NEB) for 3 h at 55 °C and crosslinks were reversed overnight at 65 °C ...
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bioRxiv - Biophysics 2024Quote: ... 0.5 mg/ml BSA (NEB), 0.5 mg/ml tRNA (Ambion) ...
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bioRxiv - Biophysics 2024Quote: ... and the second fragments for ligation with T4 DNA ligase (NEB) following the manufacture manual ...
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bioRxiv - Synthetic Biology 2024Quote: ... The DNA fragments were purified using the Monarch PCR & DNA Cleanup Kit (NEB). We assembled the plasmids through Gibson Assembly using the NEBuilder HiFi DNA Assembly Master Mix (NEB ...
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bioRxiv - Synthetic Biology 2024Quote: ... and T4 Ligase (NEB #M0202) was performed as recommended by the manufacturer (using recommendations for “simple-moderate assembly difficulty”) ...
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bioRxiv - Synthetic Biology 2024Quote: ... We assembled the plasmids through Gibson Assembly using the NEBuilder HiFi DNA Assembly Master Mix (NEB) and followed the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2024Quote: ... After 5 PCR cycles using Q5 polymerase (NEB #M0491) with 5X GC Buffer with a melting temperature of 72°C ...
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bioRxiv - Synthetic Biology 2024Quote: ... The variants were created from the wild type plasmids using the Q5 Site-Directed Mutagenesis Kit (NEB) and transformed into MegaX cells made chemically competent (Mix & Go E ...
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bioRxiv - Synthetic Biology 2024Quote: ... DpnI treated PCR products were purified with silica column (FastGene Gel/PCR Extraction Kit, Nippon Genetics Co., Ltd., Tokyo, Japan) and then HiFi assembly reaction (New England Biolabs, Ipswich, MA) was carried out to connect each end of linear DNA products ...
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bioRxiv - Synthetic Biology 2024Quote: ... Golden Gate Assembly using PaqCI (NEB #R0745) and T4 Ligase (NEB #M0202 ...
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bioRxiv - Synthetic Biology 2024Quote: ... PCRs were performed with Q5 High-fidelity DNA polymerase (NEB, M0491L) plus GC buffer with the following reaction conditions ...
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bioRxiv - Plant Biology 2024Quote: ... The RNA samples were treated with DNase I (NEB, RNase-free, Ipswich, MA, USA) and approximately 1 μg of RNA was reverse transcribed using SuperScriptTM II RT (Invitrogen ...
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bioRxiv - Plant Biology 2024Quote: ... DNA libraries were prepared using the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina® (New England Biolabs Cat. No. E7645) and the NEBNext® Multiplex Oligos for Illumina® (New England Biolabs Cat ...
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bioRxiv - Plant Biology 2024Quote: ... in an N-terminal fusion (MIROs are tail anchored proteins) with mCherry that was generated by Gibson assembly (New England Biolabs, Ipswich ...
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bioRxiv - Plant Biology 2024Quote: ... In-frame deletions and substitutions were generated using a Q5 Site-Directed Mutagenesis Kit (New England Biolabs, Hitchen UK) using the primers listed in Table S1 ...
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bioRxiv - Plant Biology 2024Quote: ... RNA (1 µg per sample) was transcribed to cDNA by the Protoscript®II reverse transcriptase (NEB). clpP ...
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bioRxiv - Synthetic Biology 2024Quote: ... and Bsu36I (NEB) yielded a DNA fragment with UMI and the SrIRED gene ...
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bioRxiv - Plant Biology 2024Quote: ... The Illumina cDNA library was prepared using a NEBNext Ultra II RNA Library Prep Kit (NEB, Ipswich, MA, USA) according to the standard experimental protocol ...
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bioRxiv - Plant Biology 2024Quote: ... CLAMT1c-Iso2 (Supplementary table 6) were amplified by Phusion polymerase (New England Biolabs) from cDNA (CLAMT1b ...