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Citations for New England Biolabs :
251 - 300 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2024Quote: ... with Phusion high-fidelity DNA Polymerase Kit (NEB), Common sgRNA-R and Primer 1 or Primer 2 (see below).
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bioRxiv - Genomics 2024Quote: ... A-tailing was done with 1X NEBuffer 2 (New England Biolabs), 0.2 mM dATP ...
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bioRxiv - Genomics 2024Quote: ... then treated with the End Repair/dA-Tailing Module (NEB, E7442L) and Ligation Module (NEB ...
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bioRxiv - Genomics 2024Quote: ... and hydroxyl repair was performed using T4 Polynucleotide Kinase (PNK; NEB). Purified RNAs were subsequently ligated with the 5’ RNA adaptor (RA5 ...
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bioRxiv - Genomics 2024Quote: ... Target loci were amplified from purified gDNA by PCR using NEBNext Ultra II Q5 Master Mix (#M0544X, NEB). Adapters for dual-indexed Illumina sequencing were attached in a second PCR step ...
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bioRxiv - Genomics 2024Quote: ... Purple (6X; New England Biolabs, cat. no. B7024)
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bioRxiv - Evolutionary Biology 2024Quote: ... intermedium cells using the Monarch® Total RNA Miniprep Kit (New England Biolabs). For the nutrient medium condition ...
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bioRxiv - Genomics 2024Quote: ... The linearized plasmid library and barcoding oligo were assembled with NEBuilder HiFi DNA Assembly Master Mix (NEB #E2621) at a 1:5 molar ratio ...
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bioRxiv - Genomics 2024Quote: ... 975 μL of pre-warmed 10-beta/Stable Outgrowth Medium was added (NEB #B9035) to each cuvette ...
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bioRxiv - Genomics 2024Quote: ... The digest product was run on a 1% agarose gel and the band was excised and purified using a Monarch DNA Gel Extraction Kit (NEB #T1020) following the manufacturer’s recommended protocol ...
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bioRxiv - Genomics 2024Quote: The ligation product was purified using a Monarch PCR & DNA Cleanup Kit (NEB #T1030) following the manufacturer’s recommended protocol ...
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bioRxiv - Genomics 2024Quote: Genomic DNA was extracted from each fraction using a Monarch Genomic DNA Purification Kit (NEB #T3010). Barcode sequences were amplified from gDNA using a two-step protocol ...
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bioRxiv - Genomics 2024Quote: ... coli (NEB #C3020). Briefly ...
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bioRxiv - Genomics 2024Quote: ... The library construct was ligated between the AflII (NEB #R0520) and NheI (NEB #R3131 ...
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bioRxiv - Genomics 2024Quote: ... and 500 uM angelicin prior to digestion with BciVI (NEB). 2.5 micrograms of DNA modified at each angelicin concentration were cut with either 4 or 8 units of restriction enzyme ...
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bioRxiv - Genomics 2024Quote: ... The ligation was performed with T4 Ligase (NEB #M0202) at 16°C for 16 hours.
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bioRxiv - Genomics 2024Quote: ... and NheI (NEB #R3131) sites in pJLS84v2 ...
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bioRxiv - Genomics 2024Quote: ... 1 μg of the step two plasmid library was linearized with SpeI-HF (NEB #R3133) at 37°C for 16 hours ...
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bioRxiv - Genomics 2024Quote: The dual pegRNA lentiviral vectors with pre-integrated co-selection pegRNAs were prepared for pegRNA library cloning by BsmBI-digestion and Quick CIP (#M0525L, NEB) treatment followed by gel purification ...
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bioRxiv - Genomics 2024Quote: Exonuclease I (New England Biolabs, cat. no. M2903)
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bioRxiv - Genomics 2024Quote: dNTPs (New England Biolabs, Ipswich, MA, USA; cat. no. N0477L)
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bioRxiv - Genomics 2024Quote: Dra I restriction enzyme (New England Biolabs, cat. no. R0129)
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bioRxiv - Genomics 2024Quote: Exonuclease I Reaction Buffer (New England Biolabs, cat. no. B0293)
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bioRxiv - Genomics 2024Quote: ... gRNAs were phosphorylated with a T4 Polynucleotide Kinase (NEB, M0201) for 30 minutes at 37°C followed by inactivation at 95°C for 5 minutes ...
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bioRxiv - Genomics 2024Quote: ... 400 ng genomic DNA of each sample was used to construct whole-genome DNA libraries with an NEBNext Ultra II FS DNA Library prep kit (NEB) and NEBNext Multiplex Oligos for Illumina (NEB) ...
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bioRxiv - Genomics 2024Quote: ... and NEBNext Multiplex Oligos for Illumina (NEB). DNA fragments were size-selected for an average insert size of 450 bp ...
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bioRxiv - Genomics 2024Quote: ... the DNA was added to 4.5 µL of 10X NEBuffer 4 (New England Biolabs), uridine diphosphate-6-azideglucose (UDP-6-N3-Glu ...
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bioRxiv - Genomics 2024Quote: ... Two 500 ng reaction tubes of DLE1-labeled DNA were each mixed with 4 µL of 10X CutSmart buffer (New England Biolabs), 60 µM of lab-made synthetic AdoYnATTO643 (Margalit et al ...
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bioRxiv - Genomics 2024Quote: ... 30 units of T4 β-glucosyltransferase (New England Biolabs) and ultra-pure water in a final volume of 45 µL ...
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bioRxiv - Genomics 2024Quote: ... pX458 and pX459 vectors were previously digested 2hours at 37°C by BbsI (NEB, R0539) and dephosphorylated 10 minutes at 37°C using Quick CIP (NEB ...
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bioRxiv - Genomics 2024Quote: ... and dephosphorylated 10 minutes at 37°C using Quick CIP (NEB, M0525). The vectors were then purified with PCR CleanUp kit (Macherey Nagel ...
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bioRxiv - Genomics 2024Quote: rCutSmart™ Buffer (New England Biolabs, cat. no. B6004)
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bioRxiv - Genomics 2024Quote: ... The ligation of vectors with gRNA inserts was performed overnight at 4°C using T4 DNA ligase (NEB, M0202). The ligation products were then transformed ...
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bioRxiv - Genomics 2024Quote: ... 1 µL of M.TaqI (10 units/µL; New England Biolabs) and ultrapure water in a total volume of 40 µL ...
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bioRxiv - Evolutionary Biology 2024Quote: ... with NEBNext Multiplex Oligos for Illumina (Dual Index Primers Sets 1 and 2) (NEB #E7600S and #E7780S), with two-sided size selection around a mode of 480 base pairs ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Genomic DNA libraries were constructed using the NEBNext Ultra II DNA Library Prep Kit (NEB #E7645L) with NEBNext Multiplex Oligos for Illumina (Dual Index Primers Sets 1 and 2 ...
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bioRxiv - Genomics 2024Quote: ... treated with USER enzyme (NEB) and amplified by PCR with barcoded universal primers NEBNext Multiplex Oligos for Illumina (NEB) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... One microliter was used as DNA matrix to produce PCR amplicons with the Phusion High Fidelity Taq Polymerase (#M0530; NEB) and new primers (Salten-pb1-3183-F and Salten-pb1-4136-R ...
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bioRxiv - Evolutionary Biology 2024Quote: ... PCR products were then introduced into pBBR1-MCS2 plasmid at XhoI and HindIII restriction sites (#R0146 and #R0104; New England Biolabs NEB). We then transformed (thermal shock ...
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bioRxiv - Evolutionary Biology 2024Quote: ... PCR products were then introduced into pBBR1-MCS2 plasmid at XhoI and HindIII restriction sites (#R0146 and #R0104; New England Biolabs NEB). We then transformed (thermal shock ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Amplicons were obtained using the Q5 high-fidelity DNA polymerase (#M0491; New England Biolabs NEB) and sent for Sanger sequencing to Eurofins Genomics France (Nantes ...
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bioRxiv - Evolutionary Biology 2024Quote: ... we multiplexed the different DNA extractions using the NebNext Ultra II FS Library Prep Kit (#E7805, #E6440 and #E6177; NEB). Briefly ...
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bioRxiv - Evolutionary Biology 2024Quote: ... France) the amplicon obtained from PCR using Q5 high-fidelity DNA polymerase (#M0491; New England Biolabs NEB) and the primers Salten-pb1-3183-F / Salten-pb1-4136-R primers (TableS1) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Amplicons were obtained using the Q5 high-fidelity DNA polymerase (#M0491; New England Biolabs NEB) and sent for Sanger sequencing to Eurofins Genomics France (Nantes ...
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bioRxiv - Evolutionary Biology 2024Quote: ... pBBR1-E and pBBR-EV were extracted using Monarch Plasmid Miniprep kit (#T1010; NEB) and following supplier’s instructions ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Successful amplicons were purified using the Exo-CIP Rapid PCR Cleanup Kit (New England BioLabs®) and sent for bi-directional Sanger sequencing with Eurofins Genomics.
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bioRxiv - Evolutionary Biology 2024Quote: ... we first confirmed the presence of mutations in the gene coding for pb1 in evolved phage populations by sequencing (Sanger; Eurofins, Nantes, France) the amplicon obtained from PCR using Q5 high-fidelity DNA polymerase (#M0491; New England Biolabs NEB) and the primers Salten-pb1-3183-F / Salten-pb1-4136-R primers (TableS1) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 3’ ends were repaired with adenine and Illumina methylated sequence adapters for NEBNext Ultra library preparation were ligated (New England Biolabs, Ipswich, MA, USA). To quantify bisulfite conversion efficiency ...
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bioRxiv - Evolutionary Biology 2024Quote: ... or four (white shavenbaby experimental) sgRNA targeting the first or second exon (100 ng/μl) and CAS9 protein (EnGen Spy Cas9 NLS from NEB, Catalog # M0646M). The distribution of the major trichome height for the white control was normal (Shapiro-Wilk normality test W = 0.9637 ...
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bioRxiv - Evolutionary Biology 2024Quote: ... The PCR reaction was then used as a template for in vitro transcription using EnGen® sgRNA Synthesis Kit (NEB), and the MEGACLEAR Transcription Clean-Up KIT (Invitrogen ...