Labshake search
Citations for New England Biolabs :
251 - 300 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Synthetic Biology 2024Quote: ... standard ligations were performed using T4 DNA ligase (NEB), while Gibson assembly reactions used the NEBuilder(R ...
-
bioRxiv - Synthetic Biology 2024Quote: ... 2µl T4 RNA ligase buffer (NEB), 1.9µl H2O ...
-
bioRxiv - Synthetic Biology 2024Quote: ... pMTB2-NLS-Cas9-2a-Citrine was digested using MfeI and XhoI restriction enzymes (NEB). Insert was amplified by PCR and digested with BbsI (NEB ...
-
bioRxiv - Developmental Biology 2024Quote: ... and the rbm8aΔ3 allele by Hinf1 (R0155S, NEB) while the rbm8aΔ5 allele stayed intact upon Xmn1 or Hinf1 digest.
-
bioRxiv - Synthetic Biology 2024Quote: All PCRs were carried out using Phusion High-Fidelity PCR Master Mix with GC Buffer (NEB), gel extractions using the QIAquick Gel Extraction Kit (QIAGEN ...
-
bioRxiv - Synthetic Biology 2024Quote: ... purple (NEB) followed by loading of 2µl mixture into a 2% agarose gel ...
-
bioRxiv - Cell Biology 2024Quote: ... using the HiFi Assembly kit (NEB). NEBuilder Assembly Tool 2.0 was used to design RRM1-2 primers with 21-bp overlap with the destination vector ...
-
bioRxiv - Developmental Biology 2024Quote: ... linearized with EcoRI (R0101S, NEB), T7 polymerase for IVT)182 ...
-
bioRxiv - Developmental Biology 2024Quote: ... 47 ml water) and 4 µL 10mg/ml Proteinase K (P8108S, NEB). The samples were incubated for 8 hours at 55 °C followed by 10 min at 98 °C to inactivate the Proteinase K ...
-
bioRxiv - Cell Biology 2024Quote: ... PCR amplicons were loaded on to 1% (w/v) agarose gels stained with Midori Green Advance DNA stain (Geneflow #S6-0022) alongside 100 bp ladder (NEB #N3231S), before submitting for DNA Sanger sequencing (Source Genomics ...
-
bioRxiv - Cell Biology 2024Quote: ... The resulting amplicon was used as starting material for in vitro transcription using HiScribe T7 High Yield RNA Synthesis Kit (NEB, E2040S). Finally ...
-
bioRxiv - Developmental Biology 2024Quote: ... Poly(A) mRNA selection and library preparation was performed using NEBNext Ultra II RNA Kit (NEB). Sequencing was performed at Boston University Microarray and Sequencing core ...
-
bioRxiv - Developmental Biology 2024Quote: ... Complementary DNA (cDNA) was prepared from total RNA (5 μg) by reverse transcription using LunaScript® RT SuperMix Kit (NEB). qPCR reactions were performed using Power SYBR Green Master Mix (Thermo ...
-
bioRxiv - Developmental Biology 2024Quote: ... PCR reactions with Q5 high fidelity polymerase (NEB) were carried out as follows ...
-
bioRxiv - Developmental Biology 2024Quote: ... Reactions were purified with a PCR purification kit (NEB). Approximately 120–150 ng of DNA was used as a template for a T7 in vitro transcription (IVT ...
-
bioRxiv - Developmental Biology 2024Quote: ... IVT sgRNA products were purified Monarch PCR and DNA Cleanup Kit (NEB) and quantified ...
-
bioRxiv - Developmental Biology 2024Quote: ... DNA was then purified with Monarch PCR & DNA Cleanup Kit (New England BioLabs). Libraries were prepared using Q5 High-Fidelity 2X Master Mix NEB ...
-
bioRxiv - Developmental Biology 2024Quote: ... Amplified libraries were purified with Monarch PCR & DNA Cleanup Kit (New England BioLabs) and sequences Illumina NextSeq 2000 system at Boston University Microarray and Sequencing core ...
-
bioRxiv - Systems Biology 2024Quote: ... PLP ligation was performed for 4 hours at room temperature using 0.5 U/µl SplintR Ligase (NEB, M0375), 1x Ampligase ligase buffer ...
-
bioRxiv - Systems Biology 2024Quote: RCA was performed with 0.5 U/μL Phi29 polymerase (NEB, M0269L), 1x Phi29 polymerase buffer ...
-
bioRxiv - Synthetic Biology 2024Quote: ... PCR reactions were performed utilizing the Q5® Hot Start High-Fidelity 2X Master Mix (New England Biolabs). Plasmids were sanger-sequenced to confirm the presence of all inserts ...
-
bioRxiv - Genomics 2024Quote: Experiments in T-47D and LNCaP cells were conducted using restriction enzyme MboI (New England Biolabs). Crosslinked chromatin was sonicated using Covaris E220 with the settings of fill level=10 ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... Custom P1 adapters containing unique 7-bp barcodes (Hohenlohe, Bassham, Currey, & Cresko, 2012) were ligated to each individual sample with T4 ligase (New England Biolabs). The DNA from all uniquely barcoded individuals in a library was pooled at equimolar concentration ...
-
bioRxiv - Developmental Biology 2024Quote: ... a mix containing 18µM sgRNA and 12µM Cas9 protein (EnGen™ Spy Cas9-NLS S. pyogenes, NEB Cat# M0646T) was incubated for 10 min at room temperature ...
-
bioRxiv - Developmental Biology 2024Quote: ... Cas9 Nuclease Reaction Buffer (NEB Cat# B0386A) was added to obtain a final 45µM sgRNA stock solution ...
-
bioRxiv - Developmental Biology 2024Quote: ... Libraries were prepared from 200 ng DNA using the NEB Enzymatic Methyl-Seq kit (NEB #E7120) following the manufacturer’s instructions ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... We digested genomic DNA with restriction enzymes EcoRI and MseI (New England Biolabs) and ligated Illumina primer and adapter sequences to the cut sites with an individual 8-nucleotide barcode on the EcoRI side ...
-
bioRxiv - Evolutionary Biology 2024Quote: ... 1 U of Q5 HotStart Taq polymerase (New England Biolabs) in 1 X Q5 buffer ...
-
Upregulated GIRK2 counteracts ethanol-induced changes in excitability & respiration in human neuronsbioRxiv - Neuroscience 2024Quote: ... using BsmB1-v1 (NEB, discontinued) in an all-in-one T7 ligase (NEB#M0318S ...
-
bioRxiv - Biophysics 2024Quote: ... Microexon 4 sequence (nucleotides 1258-1281) was deleted by PCR on the pBSK-nCPEB4 plasmid using Gibson Assembly® Master Mix (New England Biolabs, E2611S), following the manufacturer’s instructions ...
-
bioRxiv - Developmental Biology 2024Quote: ... The Tg(UAS:GFP-P2A-ppp3ccacA) has been generated by ligation (KLD kit, NEB) of PCR fragments GFP-P2A-ppp3ccacA (IM459/IM460 ...
-
bioRxiv - Developmental Biology 2024Quote: ... Template pDNA was linearized and ARCA-capped IVT-mRNA was produced using the T7 HiScribe kit from NEB, pseudo-uridine was incorporated into the reaction ...
-
bioRxiv - Developmental Biology 2024Quote: ... and purified using the Monarch RNA Cleanup Kit (NEB) and stored at −80 °C before use ...
-
bioRxiv - Developmental Biology 2024Quote: ... The donor DNA construct containing both homology arms (pENTR-HAL-mCherry-HAR; 5853 bp) was created using the NEBuilder HiFi DNA Assembly Master Mix (NEB #E2621). The following primers were used for amplifying each DNA fragment.
-
bioRxiv - Developmental Biology 2024Quote: ... constructs cloned into the pG1-HSP70 plasmids were extracted using XhoI/NotI restriction enzymes and inserted into the XhoI/NotI pre-digested pG1-flk1 vector using DNA T4 ligation (NEB, Cat # M0202S).
-
bioRxiv - Developmental Biology 2024Quote: ... coli (NEB, Cat # C2987) and resistant clones then used for miniprep plasmid preparations (Nucleospin plasmid ...
-
bioRxiv - Developmental Biology 2024Quote: ... All constructs generated in this study were obtained using the Gibson assembly assay (NEB, Cat # E2611S).
-
bioRxiv - Biophysics 2024Quote: ... using Gibson Assembly® Master Mix (New England Biolabs, E2611S) and following the manufacturer’s instructions ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... or the Q5 Site-Directed Mutagenesis Kit (New England Biolabs). We verified the sequences of plasmid DNAs purified from transformed E ...
-
bioRxiv - Cell Biology 2024Quote: ... 0.1% BSA) with or without Short Cut RNase III (New England Biolabs; 1:150) for 90 min at 37°C ...
-
bioRxiv - Cell Biology 2024Quote: ... cells were treated with a 1:150 dilution of RNase III (New England Biolabs) in low-salt buffer (50 mM Tris-HCl pH 7.6 ...
-
bioRxiv - Cell Biology 2024Quote: Library preparation for Nanopore sequencing was carried out using the NEBNext Companion Module for Oxford Nanopore Technologies Ligation Sequencing (New England Biolabs), the Ligation Sequencing Kit V14 and the Flongle Sequencing Expansion (Oxford Nanopore Technologies) ...
-
bioRxiv - Cell Biology 2024Quote: ... coli-derived recombinant human histone H1 (NEB, Ipswich, MA, USA #M2501), H2A (NEB ...
-
bioRxiv - Cell Biology 2024Quote: ... or H4 (NEB, #M2504) (NEB’s products are proteases-free ...
-
bioRxiv - Cell Biology 2024Quote: ... H3.1 (NEB, #M2503), or H4 (NEB ...
-
bioRxiv - Cell Biology 2024Quote: ... H2A (NEB, #M2502), H2B (NEB ...
-
bioRxiv - Cell Biology 2024Quote: ... H2B (NEB, #M2505), H3.1 (NEB ...
-
bioRxiv - Cell Biology 2024Quote: ... or H4 (NEB, #M2504) (NEB’s products are proteases-free ...
-
bioRxiv - Cell Biology 2024Quote: ... containing a 5′-Biotin-PC group and a 3’-OH (Sup. Table. 2) were ligated to the 5′ end of RNAs using T4 RNA ligase (NEB, Cat #M0204S) for 3 hours at 37°C ...
-
bioRxiv - Physiology 2024Quote: ... mRNA Magnetic Isolation Module was followed by library preparation using the NEBNext Ultra II RNA Directional Library Prep Kit for Illumina (New England BioLabs). Single read sequencing took place on a NextSeq 2000 System (Illumina) ...