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Citations for New England Biolabs :
601 - 650 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... using Gibson Assembly (NEB E2611) with a GSSG linker between the protein and the tag ...
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bioRxiv - Microbiology 2024Quote: ... cDNA was obtained using reverse transcription PCR (Yugong Biolabs, China). The reaction system was prepared according to the kit’s requirements (Yugong Biolabs ...
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bioRxiv - Microbiology 2024Quote: ... The reaction system was prepared according to the kit’s requirements (Yugong Biolabs) and fluorescence quantitative analysis was performed using the ABI 7500 PCR system (Applied Biosystems) ...
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bioRxiv - Genomics 2024Quote: ... and 5′ hydroxyl repair with PNK (NEB). The 5′ adapter was ligated with T4 RNA ligase (NEB) ...
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bioRxiv - Neuroscience 2024Quote: ... and then restriction digested using HindIII and PspXI (New England BioLabs, Ipswich, MA) to create compatible sticky ends ...
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bioRxiv - Developmental Biology 2024Quote: ... Fragments were digested using EcoRI-HF (New England Biolabs) and purified using the MinElute Cleanup kit (Qiagen) ...
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bioRxiv - Developmental Biology 2024Quote: ... pUAST vector was double digested using EcoRI-HF and HpaI (New England Biolabs), then the digested vector was run in an electrophoresis gel ...
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bioRxiv - Developmental Biology 2024Quote: The mRNA isolation utilized the NEBNext Poly(A) mRNA Magnetic Isolation Kit (NEB, #E7490) with 1 μg of total RNA ...
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bioRxiv - Developmental Biology 2024Quote: ... RNA-seq libraries were generated using the NEBNext Ultra Directional RNA Library Prep Kit (NEB, #E7420L), followed by sequencing on an Illumina Hiseq 4000 system ...
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bioRxiv - Cell Biology 2024Quote: ... under a CamKII promoter were both restriction digested with AgeI and the HALO protein was ligated with Quick Ligase (NEB) into the ER-GCaMP yielding the CamKII ER-Halo-GCaMP6-150 ...
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bioRxiv - Cell Biology 2024Quote: ... The ER-Halo-GCaMP6-150 was PCR amplified and the resulting fragment was combined with the hDLXI56i backbone with HiFi (NEB). The hDLXI56i enhancer was originally obtained from CN1851-rAAV-hI56i-minBglobin-iCre-4×2C-WPRE3-BGHpA (Graybuck et al. ...
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bioRxiv - Cell Biology 2024Quote: ... and 0.4 U/μL Terminal Deoxynucleotidyl Transferase (NEB) were mixed in 1× Terminal Transferase Reaction buffer and incubated at 37°C overnight ...
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bioRxiv - Cell Biology 2024Quote: crRNAs were synthesized using HiScribe T7 Quick High Yield RNA Synthesis Kit (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... cDNA synthesis was achieved with the NEBNext RNA First Strand Synthesis and NEBNext Ultra Directional RNA Second Strand Synthesis Modules (New England BioLabs). The remaining steps of library preparation were done using and the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England BioLabs) ...
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bioRxiv - Cell Biology 2024Quote: ... The remaining steps of library preparation were done using and the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England BioLabs). Adapters and PCR primers were purchased from New England BioLabs ...
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bioRxiv - Cell Biology 2024Quote: ... and PCR was performed using Q5® High-Fidelity 2X Master Mix (NEB). The splicing efficiency is monitored using RT-PCR with primers located on two adjacent exons ...
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bioRxiv - Cell Biology 2024Quote: ... The 3′ cDNA adapter was ligated by T4 RNA ligase 1 (NEB) by incubating at 25 °C for 16h ...
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bioRxiv - Cancer Biology 2024Quote: ... The oligo was obtained from IDT DNA and amplified by PCR using Phusion Polymerase (NEB F630) and purified for in vitro transcription using the MEGAshortscript T7 kit (Invitrogen AM1333) ...
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bioRxiv - Cancer Biology 2024Quote: ... by PCR amplification with the indicated primers (Key Resources table) followed by a digestion with Bam HI and Xba I enzymes (New England Biolabs) for 37°C 2 h ...
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bioRxiv - Cancer Biology 2024Quote: ... 1ug of DNase-treated RNA was then used for NEB Next Poly(A) mRNA isolation and subsequent next generation sequencing library construction (NEB) following factory procedures ...
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bioRxiv - Cancer Biology 2024Quote: ... 1% sodium dodecyl sulfate) and reverse crosslinked with proteinase K overnight at 65C and subsequently treated with RNAse A (NEB) for 1 hour at 37°C ...
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bioRxiv - Cancer Biology 2024Quote: The purified DNA was fragmented using the Covaris M220 and the libraries were constructed using the NEBNext Ultra II DNA Library Prep Kit (New England Biolabs) for next generation sequencing ...
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bioRxiv - Cell Biology 2024Quote: ... the two fragments were assembled using a Gibson assembly mix (NEB, no. E2611S) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... 16.4 mL elution was taken into 40 mL ligation reaction (13 Quick ligase buffer [NEB], 4000U Quick ligase [NEB] ...
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bioRxiv - Cell Biology 2024Quote: ... 20 mL ERA reaction (13 T4 DNA ligase buffer [NEB], 0.5 mM dNTPs, 0.25 mM ATP, 2.75% PEG 4000, 6U T4 PNK [NEB] ...
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bioRxiv - Cell Biology 2024Quote: ... 20 mL ERA reaction (13 T4 DNA ligase buffer [NEB] ...
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bioRxiv - Cell Biology 2024Quote: ... and TE buffer, DNA was reverse crosslinked by incubating beads in Elution buffer (1% SDS, 0.1M NaHCO3 and Proteinase K (NEB)) ...
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bioRxiv - Microbiology 2024Quote: ... The Gibson Assembly master mix was obtained from NEB (E2611S). DNA fragments were amplified by PCR from bacterial genomic DNA or from DNA synthesized by TWIST Bioscience ...
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bioRxiv - Cell Biology 2024Quote: ... Following BamHI (NEB #R3136)-XhoI (NEB #R0146) digest of the MDM2 PCR product and destination vector ...
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bioRxiv - Cell Biology 2024Quote: ... Following BamHI (NEB #R3136)-XhoI (NEB #R0146 ...
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bioRxiv - Cell Biology 2024Quote: ... following NdeI (NEB #R0111S)-XhoI (NEB #R0146) digest.
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bioRxiv - Cell Biology 2024Quote: ... following NdeI (NEB #R0111S)-XhoI (NEB #R0146 ...
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bioRxiv - Cell Biology 2024Quote: ... ligations were performed with T4 ligase (NEB #M0202). Ligated plasmids were transformed into competent XL-1 blue cells (Agilent Technologies #200249) ...
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bioRxiv - Synthetic Biology 2024Quote: ... and PCR products were purified with Monarch PCR and DNA Cleanup kit (New England Biolabs, Ipswich, Massachusetts, USA).
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bioRxiv - Systems Biology 2024Quote: Approximately 100 ng of each RNA sample was used to create Illumina sequencing libraries using a NEBNext Ultra II Directional RNA Library Prep Kit for Illumina with NEBNext Poly(A) mRNA Magnetic Isolation Module (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Systems Biology 2024Quote: ... Approximately 100 ng of each DNA sample was used to create Illumina sequencing libraries using a NEBNext Ultra II FS DNA Library Prep Kit (New England Biolabs (NEB), E7805S) ...
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bioRxiv - Systems Biology 2024Quote: ... After PCR amplification by Phusion DNA polymerase (NEB) and TBS380 (Picogreen ...
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bioRxiv - Systems Biology 2024Quote: 50 ng RNA of each sample were reverse transcribed and barcoded by using the PCR-cDNA barcoding kit (SQK-PCB111.24) and NEBNext Companion Module (NEB E7180L). Libraries were then sequenced on the Nanopore PromethION platform.
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bioRxiv - Systems Biology 2024Quote: ... Approximately 100 ng of each DNA sample was used to create Illumina sequencing libraries using a NEBNext Ultra II FS DNA Library Prep Kit (New England Biolabs (NEB), E7805S) ...
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bioRxiv - Cancer Biology 2024Quote: ... coli (High Efficiency) (NEB, C2987H) and Stbl3 Competent E ...
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bioRxiv - Plant Biology 2024Quote: ... The sgRNA was first synthesised using the EnGen sgRNA synthesis kit New England Biolabs (NEB #E3322) before mixing with the Cas9-NLS to form the RNP complex ...
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bioRxiv - Plant Biology 2024Quote: ... The resulting cDNA was then amplified using Phusion High-Fidelity DNA Polymerase (New England Biolabs). GAPDH level was utilized as the transcriptional control ...
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bioRxiv - Developmental Biology 2024Quote: ... runx1 (using pBS-runx1a, linearized with HindIII (R0104S, NEB), T7 polymerase for IVT)183 (a kind gift from Dr ...
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bioRxiv - Cancer Biology 2024Quote: ... was conducted using the Monarch® Genomic DNA Purification Kit (NEB, Cat # T3010S). Primers were procured from Integrated DNA Technologies and designed with the PrimerQuest™ Tool (IDT ...
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bioRxiv - Cancer Biology 2024Quote: ... DNA fragments were then cloned into a lentiviral expression vector with a Gibson assembly product (NEB, E2611S). DNA sequencing was performed to confirm accuracy of the vector ...
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bioRxiv - Cancer Biology 2024Quote: ... Bound antibodies were detected by incubation with DyLight 800-conjugated secondary antibodies (New England BioLabs), and analysed using an InnoScan 710-IR scanner (Innopsys) ...
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bioRxiv - Cancer Biology 2024Quote: ... Dot blot samples were prepared from 1 µg gDNA with or without RNaseH1 2500 U treatment (NEB, M0297) O/N 37°C.Samples were denatured in Denaturation Buffer (NaOH 400 mM ...
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bioRxiv - Neuroscience 2024Quote: ... Illumina library was made from polyA RNA with NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (NEB) according to the manufacturer’s manual74 ...
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bioRxiv - Cancer Biology 2024Quote: ... PCR was performed using unique dual index primer pairs (NEBNext Multiplex Oligos for Illumina from New England BioLabs, # E6440S) according the following parameters ...
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bioRxiv - Cancer Biology 2024Quote: ... followed by Klenow fragment exo-A-tailing (NEB, cat. M0212), adaptors ligation with Quick Ligation kit (NEB ...