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Citations for New England Biolabs :
601 - 650 of 10000+ citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... and 20U RNase inhibitor (NEB). The translation reactions were incubated in 384 well plates at 30°C for 1hr ...
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bioRxiv - Biochemistry 2024Quote: ... Parental plasmids were digested by adding 5 µL 10x cutsmart buffer (NEB), and 1 µL DpnI (NEB ...
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bioRxiv - Biochemistry 2024Quote: ... A colony PCR using Taq Polymerase (New England Biolabs) on positive resistant colonies can help identifying false positives for Ampicillin resistance ...
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bioRxiv - Biochemistry 2024Quote: ... coli (Suppl. Table 1) were cloned into a pBAD24 with ampicillin resistance using the USER cloning method (New England Biolabs, Table 2). All sequences were cloned including a 10x histidine tag located in the N-terminus for BtuG1-3 and the C-terminus for BtuG2 and all its mutants ...
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bioRxiv - Biochemistry 2024Quote: ... reactions were quenched on ice with STOP Buffer (0.4 U proteinase K (NEB), 18 mM EDTA ...
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bioRxiv - Biophysics 2024Quote: ... coli NiCo21(DE3) strain (NEB). Cells were grown at 37°C supplemented with 2XYT medium (Melford ...
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bioRxiv - Biophysics 2024Quote: ... The isolated RNA underwent enzymatic capping (NEB) and polyadenylation (Thermo Fisher) ...
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bioRxiv - Biochemistry 2024Quote: ... Total RNA was extracted using the Monarch Total RNA Miniprep Kit (T2010S, New England Biolabs) and cDNA was synthesized using the SuperScript IV First-Strand Synthesis System (18091050 ...
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bioRxiv - Biochemistry 2024Quote: ... the collected cell pellets were processed using Monarch RNA extraction kit (New England BioLabs). The RNA samples were then quantified with nanodrop and normalized before cDNA synthesis (Lambda Biotechnologies) ...
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bioRxiv - Biochemistry 2024Quote: ... The plasmids were transformed into either BL21(DE3) (NEB) or OrigamiB(DE3 ...
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bioRxiv - Biochemistry 2024Quote: ... Fragments were assembled into the pBAD24 vector using HiFi DNA Assembly Kit (New England Biolabs, Ipswich, MA) with 1-hour ...
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bioRxiv - Biochemistry 2024Quote: ... followed by affinity-purification with amylose resin (New England Biolabs). The amylose eluates were purified further by anion exchange chromatography using Source 15Q Resin (Cytiva) ...
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bioRxiv - Biochemistry 2024Quote: ... The HDV sequence was cloned into the XbaI/EcoRV sites of the AVA421 vector to enable linearization for run-off transcription by digestion with EcoRV-HF (NEB) in a reaction containing 1X CutSmart buffer and 0.4 U/μl EcoRV-HF.
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bioRxiv - Biochemistry 2024Quote: ... a pET-derived vector that is normally used for T7 RNA polymerase-dependent protein expression (AVA421) was linearized by digestion with XbaI (NEB) in a reaction containing 1X CutSmart buffer and 0.5 U/μl XbaI to be used for run-off transcription using T7 RNA polymerase to transcribe the first 26 nucleotides following the T7 promoter sequence to generate the standard model RNA substrate ...
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bioRxiv - Biochemistry 2024Quote: ... and 1 µL DpnI (NEB) directly to the PCR reaction mixture after PCR and continued incubation for 1h at 37°C ...
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bioRxiv - Biochemistry 2024Quote: ... coli NEB C2566 cells (New England Biolabs). The cells were grown at 37°C in TB medium for HINT1 ...
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bioRxiv - Bioengineering 2024Quote: ... and mCherry inserts were generated via PCR and cloned into the linearized viral backbone as a single fusion protein using HiFi cloning mix (NEB). H2B-iRFP nuclear marker was (Addgene Plasmid #90237) ...
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bioRxiv - Biophysics 2024Quote: ... coli SHuffle T7 Express (New England Biolabs, Ipswich, MA) cells were transformed with 100 ng pET32-Trx-LqhαIT and ...
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bioRxiv - Biophysics 2024Quote: ... Monarch RNase A (NEB) (0.1 ng/mL in final concentration ...
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bioRxiv - Biochemistry 2024Quote: ... 1 µL T4 DNA Ligase (NEB), 100 ng DNA (oligo 3) ...
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bioRxiv - Biophysics 2024Quote: ... R4: 5’-TAGGTACAGTCCTCAGGTGTCTT[AmC6dT]T) were coupled with SNAP-substrate NHS ester (BG-GLA-NHS, NEB). The DNA strands at 1 mM concentration were incubated with 10 mM of BG-GLA-NHS and reactions were cleaned up using a Bio-Spin P-30 column (Bio-rad).
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bioRxiv - Biophysics 2024Quote: ... Splint ligation reaction was done by mixing DNA in equal molar ratios of 1 mM in a 20 μl reaction and annealed in 1✕ ligation buffer (NEB) over 4 hours using a thermocycler (bio-rad) ...
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bioRxiv - Biophysics 2024Quote: ... N-glycosylation was removed by a 1-hour incubation with PNGase F (500 U, NEB) and following digest with sequence-grade trypsin (Promega ...
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bioRxiv - Bioengineering 2024Quote: ... grown in 5-alpha competent Escherichia coli (NEB, Ipswich, MA) and purified using the PureYield MidiPrep System (Promega ...
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bioRxiv - Biochemistry 2024Quote: ... isolated chaperones and empty 70S ribosomes (NEB) were prepared as controls ...
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bioRxiv - Biochemistry 2024Quote: ... coli cells (NEB) were transformed with plasmids encoding β-gal RNC constructs and grown in ZYM-5052 autoinduction media 106 for 18 h at 37 °C ...
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bioRxiv - Biochemistry 2024Quote: ... deletions and point mutations were introduced using site-directed mutagenesis with Q5 or Phusion polymerases (NEB). All constructs used in this study (Table S10 ...
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bioRxiv - Bioengineering 2024Quote: ... Genotyping PCR was then conducted using Q5 polymerase (NEB) for genomic DNA and cDNA ...
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bioRxiv - Bioengineering 2024Quote: The 5’ RACE protocol using the template-switching reverse transcriptase enzyme mix from NEB was executed following the manufacturer’s guidelines ...
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bioRxiv - Bioengineering 2024Quote: ... mRNA was then reverse-transcribed using LunaScript RT-supermix (NEB #E3010). Genotyping PCR was then conducted using Q5 polymerase (NEB ...
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bioRxiv - Bioengineering 2024Quote: ... and RT enzyme mix (NEB #M0466), followed by an incubation step at 42°C for 90 minutes and then at 85°C for 5 minutes ...
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bioRxiv - Biochemistry 2024Quote: ... and parent plasmids were degraded by DpnI (NEB). All plasmids were transformed into DH5α cells.
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bioRxiv - Biochemistry 2024Quote: ... residue F31 was mutated to W31 using Q5 mutagenesis (New England Biolabs) only in the GST-tagged construct ...
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bioRxiv - Biochemistry 2024Quote: ... 25 μL of Q5 High-Fidelity DNA Polymerase (New England Biolabs #M0494S), 2.5 μL of diluted forward and reverse primer ...
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bioRxiv - Biochemistry 2024Quote: ... 0.1 mg/ml recombinant albumin (New England Biolabs), 0.1 mg/ml RNase A (QIAGEN) ...
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bioRxiv - Biochemistry 2024Quote: ... appropriate fragment of SynPOR gene was amplified with specific primers with additional fragments corresponding to amplified pET15b_AtPORB (Table S2) with Q5 polymerase (New England Biolabs) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2024Quote: ... were amplified with PCR using Q5 polymerase (New England Biolabs) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2024Quote: ... was obtained from New England Biolabs (NEB). Some deglycosylation enzymes can be seen on SDS-PAGE gels when added.
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bioRxiv - Bioengineering 2024Quote: ... PNGase F (P0710S NEB) was used ...
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bioRxiv - Bioengineering 2024Quote: ... deglycosylation mix II (P6044S NEB) was obtained from New England Biolabs (NEB) ...
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bioRxiv - Bioengineering 2024Quote: ... The extracted DNA (3 μg) was fully digested with NcoI restriction enzyme (New England Biolabs, USA), separated in a 0.8% agarose gel and blotted onto a Hybond+ Nylon membrane (Amersham Biosciences) ...
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bioRxiv - Bioengineering 2024Quote: ... a restriction digest of the nanoplasmid constructs using SSPI-HF (Cat # R3132S, New England Biolabs, Ipswich, MA) was performed ...
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bioRxiv - Bioengineering 2024Quote: Linear dsDNA templates were made via PCR amplification was done using the GD2-CAR or no CAR Control plasmid as a template using Q5 Hot Start Polymerase (Cat # M0494S, NEB, Ipswich, MA) in 50 uL reaction volumes ...
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bioRxiv - Biochemistry 2024Quote: The DNA encoding full-length β-galactosidase was amplified from NEB Escherichia coli (E ...
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bioRxiv - Biochemistry 2024Quote: ... Gibson Assembly cloning (NEB) was used to generate a set of pET21 plasmids encoding β-galactosidase ORFs upstream of a ribosome stalling sequence (WWPRIRGPPGS 90 ...
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bioRxiv - Biochemistry 2024Quote: ... coli Rosetta DE3 competent cells (NEB) to co-express full-length HIV-1 IN and the IBD ...
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bioRxiv - Biochemistry 2024Quote: KP4 or HEK293T cells were reverse transfected with preformed Cas9 RNP with EnGen Cas9 NLS (NEB), crRNA targeting RNF19B (target sequence ...
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bioRxiv - Biochemistry 2024Quote: ... DNA substrates were 5’ terminally labelled with T4 Polynucleotide Kinase (NEB, M0201S) and ATP-γ-32P (PerkinElmer ...
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bioRxiv - Biochemistry 2024Quote: ... 125 μg of λ-phage DNA was mixed with two oligos (2 μM oligo Lab07 (/5Phos/AGG TCG CCG CCC/3BioTEG) and 2 μM oligo Lab06 (/5Phos/GGG CGG CGA CCT/3BioTEG) in 1× T4 DNA ligase reaction buffer (NEB B0202S) and heated to 70°C for 15 min followed by gradual cooling to 15°C for 2 hours ...
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bioRxiv - Biochemistry 2024Quote: ... Truncated EWSR1 constructs were created by PCR-based cloning from the His-MBP-EWSR1 expression vector using inverse PCR with Phusion DNA polymerase (New England Biolabs, F530S) then DpnI digest (New England Biolabs ...