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Citations for New England Biolabs :
501 - 550 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... into the HindIII and SpeI restriction sites of the pSEVA23a1 vector79 where a hygromycin resistance cassette had been inserted in BamHI and XbaI (all restriction enzymes were from New England Biolabs). Correct cloning was confirmed by Sanger sequencing (Eurofins) ...
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bioRxiv - Microbiology 2024Quote: ... 25 ng of linearised plasmids were ligated with 75 ng of purified VH and VL by Gibson Assembly (New England BioLabs). The reaction was performed in a final volume of 5 μL ...
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bioRxiv - Microbiology 2024Quote: ... Specific probes were generated by end-labeling of oligodeoxyribonucleotides (Table S3) using T4 Polynucleotide Kinase (New England Biolabs) and [γ-32P]-ATP (Hartmann Analytic) ...
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bioRxiv - Microbiology 2024Quote: ... TAP-PCR was performed in a total volume of 25 μL containing 0.25 μL of Q5 polymerase (NEB), 5 μL of GC Enhancer (NEB) ...
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bioRxiv - Immunology 2024Quote: ... The region of interest was then amplified using Q5 2× mastermix (New England Biolabs, cat. M0492S), 500 ng of template DNA ...
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bioRxiv - Immunology 2024Quote: Poly A-enriched libraries were made using the NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB, cat. E7770) and sequenced on a NovaSeq SP with PE150 read length.
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bioRxiv - Immunology 2024Quote: Sequencing: Poly A-enriched libraries were made using the NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB, cat. E7770) and sequenced on a NovaSeq SP with PE150 read length.
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bioRxiv - Immunology 2024Quote: ... Libraries were prepared by the West Virginia University Genomics Core using the NEBNext Low Input/Single Cell RNA-Seq kit (New England Biolabs). Libraries were sequenced to 30 million reads on a Nextseq 2000 (Illumina ...
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bioRxiv - Microbiology 2024Quote: ... the pUC19-PRha-YFP-3×FLAG plasmid was inverse amplified by PCR (Q5 polymerase, NEB), omitting the yfp gene ...
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bioRxiv - Microbiology 2024Quote: ... Poly(A) mRNA was isolated via NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB) or the Ribo-Zero rRNA removal Kit (Illumina) ...
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bioRxiv - Microbiology 2024Quote: ... Then we used T7 Ribomax Express (NEB) in vitro RNA synthesis kit to generate single-stranded negative sense RNA that resembles the vRNA molecule ...
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bioRxiv - Microbiology 2024Quote: ... were prepared by cloning the codon-optimized (E.coli expression) synthetic gene fragments into pMAL-p2x vector (New England Biolabs) for periplasmic expression in BL21-CodonPlus(DE3)-RIPL (Agilent Technologies ...
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bioRxiv - Microbiology 2024Quote: ... The Monarch RNA Cleanup Kit (NEB) was used to purify the resulting RNA ...
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bioRxiv - Microbiology 2024Quote: ... and detected using anti-MBP antibodies (New England Biolabs). As a negative control ...
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bioRxiv - Microbiology 2024Quote: ... Using Gibson Assembly (New England Biolabs), the upstream and downstream regions of ttdV ...
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bioRxiv - Microbiology 2024Quote: ... the upstream homologous region was cloned into the ApaI/KpnI-HF (NEB) digested pSFS2 plasmid ...
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bioRxiv - Microbiology 2024Quote: ... digested pSFS2 plasmid using NEBuilder HiFi (NEB) as per manufacturer instructions ...
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bioRxiv - Microbiology 2024Quote: ... 5-10 µg of DNase-treated RNA samples were denatured for 3 min at 70°C and separated on a 15% (w/v) TBE-urea gel (Novex®) with a low range ssRNA ladder (New England Biolabs, USA). The gel was stained with 1x SYBR gold dye (Thermo Fisher Scientific GmbH ...
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bioRxiv - Microbiology 2024Quote: ... the upstream homologous region was cloned into the XhoI/KpnI-HF (NEB) digested pSFS2 plasmid using NEBuilder HiFi (NEB ...
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bioRxiv - Microbiology 2024Quote: ... Metagenomic sequencing libraries were prepared using the NEBNext Ultra II DNA Library Prep kit (New England Biolabs, MA, USA) with a targeted insert size of 350-400bp and Dual Index multiplex oligos ...
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bioRxiv - Microbiology 2024Quote: ... the sequence encoding amino acid residues 1 to 35 of the N-terminal end of BVG96_RS17270 (89) was PCR amplified using Q5 polymerase (NEB) and cloned via NEBuilder HiFi DNA Assembly (NEB ...
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bioRxiv - Microbiology 2024Quote: Molecular biology reagents were from NEB and chemical reagents were from Sigma unless otherwise noted ...
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bioRxiv - Microbiology 2024Quote: ... and cloned via NEBuilder HiFi DNA Assembly (NEB) into plasmid pIDMv5K-J231000-Dasher-GFP-B1006 (unpublished ...
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bioRxiv - Immunology 2024Quote: ... Immunoglobulin heavy and light chain cDNA was amplified using the Q5 High-Fidelity 2X Master mix (NEB) and pooled forward primers that bind the leader sequences of the variable regions and the reverse primers that bind the first exon of the mu and kappa constant regions ...
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bioRxiv - Microbiology 2024Quote: ... colony PCRs (GOTAQ, NEB) were performed on positive E ...
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bioRxiv - Microbiology 2024Quote: ... The sequence of the rhamnose construct starting at the ilvBN terminator and ending with the rhaBAD cassette of pCK355 [66] was amplified using Q5 polymerase (NEB), adding overlaps for AQUA cloning into pUC19 [67] ...
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bioRxiv - Molecular Biology 2024Quote: ... vanadyl ribonucleoside complex (VRC; New England Biolabs) was added to the NPB at a concentration of 10 mM ...
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bioRxiv - Microbiology 2024Quote: ... Bisulfite-converted RNAs were then end-repaired and subjected to NEBNExt Small RNA Library Prep Set for Illumina (NEB, E7330L), according to manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2024Quote: ... 20-50 ng of purified RNA was processed by NEBNext rRNA Depletion Kit (New England BioLabs, Inc.) for removing ribosomal RNA ...
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bioRxiv - Molecular Biology 2024Quote: ... LongAmp® Hot Start (New England Biolabs, USA); Phusion™ Hot Start II DNA Polymerase (Invitrogen™ ...
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bioRxiv - Molecular Biology 2024Quote: ... Q5U® Hot Start High-Fidelity DNA Polymerase (New England Biolabs, USA); NEB Next® Ultra™ II Q5® mastermix (New England Biolabs ...
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bioRxiv - Microbiology 2024Quote: ... and menA were amplified by PCR from the IR715 genome using Q5 High Fidelity Master Mix (New England Biolabs). PCR products were purified using the QIAEX II Gel Extraction Kit (Qiagen) ...
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bioRxiv - Microbiology 2024Quote: ... PCR products and vector pBRCS12 were digested with HindIII and BamHI (New England Biolabs) restriction endonucleases ...
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bioRxiv - Microbiology 2024Quote: ... Digestion products were ligated using DNA Ligase (New England Biolabs) and the plasmids were transformed into XL10 competent cells and the plasmid was purified ...
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bioRxiv - Microbiology 2024Quote: ... Clean deletions were verified via PCR using Quick-Load Taq Master Mix (New England Biolabs) or Q5 High Fidelity Master Mix ...
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bioRxiv - Molecular Biology 2024Quote: ... and LunaScript® RT SuperMix (New England Biolabs, USA), referred to as Superscript™ and LunaScript® ...
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bioRxiv - Molecular Biology 2024Quote: ... NEB Next® Ultra™ II Q5® mastermix (New England Biolabs, USA); and Platinum™ SuperFi™ DNA Polymerase (Invitrogen™ ...
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bioRxiv - Microbiology 2024Quote: ... and cDNA library preparation (NEBNext Ultra II Directional RNA Library Prep Kit - Bacteria (New England Biolabs) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... genomic DNA was digested on-beads using 1U of DNAse I (NEB M0303) at room temperature for 10 minutes ...
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bioRxiv - Immunology 2024Quote: ... A total of 1µg of the purified PCR product was used for in vitro transcription using the HiScribe T7 High Yield RNA synthesis (NEB E2040S), as recommended by the manufacturer ...
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bioRxiv - Cell Biology 2024Quote: ... 15 µl of genomic DNA (DNA concentration is around 100 ng/µl) were digested or mock with 20 units of BsrGI or BamHI restriction enzymes (New England BioLabs) for 5 hours at 37 °C ...
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bioRxiv - Developmental Biology 2024Quote: ... Total RNA was then purified with Monarch RNA Cleanup Kit (NEB) and quantified using Qubit Fluorimetric Quantification (Thermo Scientific) ...
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bioRxiv - Developmental Biology 2024Quote: ... The sorted spermatid fractions were collected in Monarch DNA/RNA protection reagent (NEB) and flash frozen in liquid nitrogen before the RNA extraction.
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bioRxiv - Developmental Biology 2024Quote: ... The sequencing libraries were prepared using NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB Catalog# E7645S) following manufacturer instructions.
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bioRxiv - Developmental Biology 2024Quote: ... The amplified pools were subjected to PCR product purification by Monarch PCR and DNA Cleanup Kit (NEB). The purified cDNAs were cloned by NEB PCR Cloning Kit and individual clones were isolated and sequenced (Azenta Life Sciences).
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bioRxiv - Developmental Biology 2024Quote: Total RNA was extracted from collected spermatids fractions with Monarch RNA extraction kit (NEB) according to manufacturers’ protocol for RNA extraction from tissue or leukocytes ...
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bioRxiv - Developmental Biology 2024Quote: ... 400 µL MNase complete buffer (85 mM Tris-HCl pH 7.5, 3mM MgCl2, 2mM CaCl2, 0.3M Sucrose, 60 IU of MNase (NEB Catalog# 0247S) was added to each chromatin preparation ...
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bioRxiv - Developmental Biology 2024Quote: The Rapid Amplification of 5’cDNA end was carried out with Template Switching Reverse Transcription protocol (Wulf et al, 2019) provided by New England Biolabs (NEB #M0466). In the first step ...
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bioRxiv - Developmental Biology 2024Quote: ... The purified cDNAs were cloned by NEB PCR Cloning Kit and individual clones were isolated and sequenced (Azenta Life Sciences).
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bioRxiv - Cell Biology 2024Quote: ... with T4 ligase (New England Biolabs). To generate the STIM1 knockdown plasmid ...