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Citations for New England Biolabs :
501 - 550 of 10000+ citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... pSNAPf vector containing SNAP-tag was purchased from NEB (N9183S) and optimised to Drosophila codon suing Genewiz (USA ...
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bioRxiv - Cell Biology 2024Quote: ... These fragments were assembled using a NEBuilder® HiFi DNA Assembly kit (NEB) to create a pET24a-anti-ALFA-std expression vector ...
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bioRxiv - Cell Biology 2024Quote: ... The amplified fragment containing SNAP was assembled with an amplified pUbq destination backbone using a NEBuilder® HiFi DNA Assembly kit (NEB) to create a pUbq-SNAP destination vector ...
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bioRxiv - Cell Biology 2024Quote: ... The pRNA destination backbone was linearised by primers s5 and s6 (Table 3) and assembled with the mScarlet-I3 fragment using a NEBuilder® HiFi DNA Assembly kit (NEB) to create a pRNA-mScarlet-I3 destination vector ...
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bioRxiv - Cell Biology 2024Quote: ... the constructs were digested and linearised by AscI (R0558S, NEB), precipitated with 10 mM sodium acetate and 7 mM EDTA in 66% ethanol overnight at −20 °C ...
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bioRxiv - Cell Biology 2024Quote: ... Synthetic oligonucleotides encoding 3xFLAG were then ligated to the 5’ position of APEX2 sequence using NEBuilder HiFi DNA assembly kit (New England Biolabs) to form the final vector pcDNA5/FRT/TO/3xFLAG-APEX2.
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bioRxiv - Molecular Biology 2024Quote: ... and 5′ end labelled using γ32P UTP (Perking Elmer) and T4 polynucleotide kinase (NEB) following manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... Sequencing libraries were prepared on beads using the Next Ultra II DNA Library Prep Kit for Illumina and Next Multiplex Oligos for Illumina (New England Biolabs) following instructions from the Arima-HiC+ for HiC (Arima Genomics) ...
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bioRxiv - Molecular Biology 2024Quote: ... 1 µg of DNA was incubated 20min at 37°C with 15 units of RNAseH1 (New England Biolabs) and first digested for 2h at 37°C with 35 units of AsiSI restriction enzyme (New England Biolabs) ...
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bioRxiv - Molecular Biology 2024Quote: ... and first digested for 2h at 37°C with 35 units of AsiSI restriction enzyme (New England Biolabs). Then ...
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bioRxiv - Molecular Biology 2024Quote: ... 10ng of purified DNA (average size 250–300bp) was used to prepare sequencing libraries with the Next Ultra II Library Prep Kit for Illumina (New England Biolabs) using the application note for “Low input ChIP-seq” ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.1pmol of each fragment were then assembled for 1h at 50°C using NEBuilder HiFi DNA Assembly master mix from the NEBuilder HiFi DNA Assembly Cloning Kit (NEB, ref E5520S). Reaction products were amplified in provided bacteria and purified using QIAprep Spin Miniprep Kit.
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bioRxiv - Genetics 2024Quote: ... added 10.0 µl Gibson Assembly Mix (NEB), and incubated at 50C for 1h ...
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bioRxiv - Molecular Biology 2024Quote: ... A 2.8 kb dsDNA fragment was obtained by PCR with Q5 Polymerase (NEB) using phosphorylated VP 1394 ...
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bioRxiv - Molecular Biology 2024Quote: ... The oligonucleotides (60nM) were hybridized to φX174 ssDNA plasmids (5.3 kb, NEB) (20nM ...
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bioRxiv - Molecular Biology 2024Quote: ... and 10 units PNK (NEB) in 50 μl PNK buffer 1X (30’ at 37°C ...
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bioRxiv - Genetics 2024Quote: ... and 0.5 µl T4 DNA ligase (NEB M0202S). Sequences of the synthesized Drop-in fragments are in Supplementary File 3.
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bioRxiv - Genetics 2024Quote: ... containing the homology arms and guides was then ligated with the digested T2A-LexA/QF2-Hsp70-3xP3-RFP with 2.5 µl 10× T4 DNA ligase buffer (NEB B0202S) and 0.5 µl T4 DNA ligase (NEB M0202S) ...
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bioRxiv - Genetics 2024Quote: gBlocks™ and oligos were ordered from Integrated DNA Technologies (IDT) and restriction enzymes were from NEB. The sequences of all gBlocks™ and oligos used to build pHDR-QF2-LexA-GAD are in Supplementary materials and methods ...
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bioRxiv - Genetics 2024Quote: ... or Q5 (NEB). In cases where the PCR product was faint ...
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bioRxiv - Neuroscience 2024Quote: ... elongated by Phusion polymerase (M0530S, New England Biolabs), and used as templates for in vitro transcription with the MAXIscript T7 Transcription Kit (AM1312 ...
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bioRxiv - Neuroscience 2024Quote: ... and then combined with the homology arms in a ligation reaction (M0202S, New England Biolabs). To synthesize ugRNA and an sgRNA targeting the rln3a gene ...
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bioRxiv - Neuroscience 2024Quote: ... and linearized by digestion with BamHI (R0136S, New England Biolabs). The template for the sst1.1 probe was a cDNA clone in a pSPORT1 vector (Argenton et al. ...
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bioRxiv - Neuroscience 2024Quote: ... following digestion of pDR274 with BsaI (R3733S, New England Biolabs). The pDR274 templates and the pDR274 vector for synthesis of the GFP bait sgRNA (Auer et al. ...
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bioRxiv - Neuroscience 2024Quote: ... was digested with XbaI (R0145S, New England Biolabs), and Cas9 RNA was synthesized using the mMESSAGE mMACHINE Transcription Kit (AM1348 ...
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bioRxiv - Genomics 2024Quote: ... digested with EcoRI-HF and NotI-HF (NEB), and then cloned into the EagI and EcoRI sites of pJK03 (AddGene #173,490 ...
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bioRxiv - Genomics 2024Quote: ... All subsequent steps were performed on that bead-bound DNA fraction including DNA ends repair with repair mix (10Х ligase NEB buffer ...
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bioRxiv - Genomics 2024Quote: ... 5U/ul Klenow (NEB #M0210S) for 90 min 900 rpm at +37 °C ...
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bioRxiv - Genetics 2024Quote: ... We ligated the PCR fragments into the vectors using T4 ligase (NEB), transformed into E ...
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bioRxiv - Genetics 2024Quote: ... which was then digested using BamHI-HF or HindIII-HF as per the manufacturer’s instructions (New England Biolabs, Ipswich, MA, USA). The percentage of targeted alleles within a cell population was measured with a Bio-Rad QX200 ddPCR machine and QuantaSoft software (v.1.7 ...
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bioRxiv - Genetics 2024Quote: ... using Gibson assembly (Gibson et al. 2009) (NEB E2611). The hsp70-3’UTR was amplified from a pCRISPaint-Gal4-Hsp70 plasmid using the following primers ...
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bioRxiv - Genetics 2024Quote: ... Gibson Assembly Mastermix (New England Biolabs, Ipswich, MA, USA) was used for the creation of all DNA repair vectors as per manufacturer’s instructions ...
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bioRxiv - Genetics 2024Quote: ... We used Phusion (NEB), Taq (Takara ...
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bioRxiv - Genetics 2024Quote: ... The ds-oligos were then ligated into BbsI-digested pCFD3 vector with T4 ligase (NEB). Following cloning ...
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bioRxiv - Genetics 2024Quote: ... 0.5 µl T4 polynucleotide kinase (NEB), and 6.5 µl dH2O ...
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bioRxiv - Genetics 2024Quote: QF2-T2A-LexA-GAD ORF PCR-amplified with FRT-QF2-fwd + FRT-lexA-GAD-rev primer pair – used Hot-Start Q5 polymerase with GC enhancer (NEB);
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bioRxiv - Developmental Biology 2024Quote: ... was mutated using Q5 site directed mutagenesis kit (NEB, primers: SP (RSV) AP2 (hTFAP2A)-CRISPR RES-fw and SP (RSV ...
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bioRxiv - Developmental Biology 2024Quote: ... T7 Endonuclease I assay (NEB) was then used to identify the presence of indel mutations in the targeted region of F1 fish ...
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bioRxiv - Developmental Biology 2024Quote: ... Library concentration was determined using NEBNext® Library Quant Kit for Illumina (New England Biolabs) or by Qubit (Thermo) ...
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bioRxiv - Developmental Biology 2024Quote: ... cDNA and library preparation were performed using NEBNext® Ultra™ II Directional RNA Library Prep kit for Illumina (New England Biolabs). Library concentration was determined using NEBNext® Library Quant Kit for Illumina (New England Biolabs ...
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bioRxiv - Evolutionary Biology 2024Quote: ... 600-800 ng of genomic DNA per-sample (depending on the library, see Table S2, Table S3) was digested using the SbfI-HF enzyme (New England Biolabs). Custom P1 adapters containing unique 7-bp barcodes (Hohenlohe ...
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bioRxiv - Molecular Biology 2024Quote: ... NEBuilder® HiFi DNA Assembly Kit (catalog no. E2621L, NEB) was used to clone the sequence of interest into plasmid backbone.
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bioRxiv - Molecular Biology 2024Quote: ... 5 µg of replicon plasmid was linearised with AscI (NEB), purified by phenol-chloroform extraction ...
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bioRxiv - Genomics 2024Quote: ... HiChIP in Ishikawa cells was conducted using restriction enzyme DpnII (New England Biolabs). Crosslinked chromatin was sonicated using an EpiShear probe-in sonicator (Active Motif ...
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bioRxiv - Genomics 2024Quote: ... The 5′ adapter was ligated with T4 RNA ligase (NEB), followed by a third biotinylated RNA enrichment ...
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bioRxiv - Genomics 2024Quote: ... in multiple ligation reactions (NEB T4 ligase). Ligation products were transformed into either 5-alpha or 10-beta electrocompetent cells (NEB ...
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bioRxiv - Genomics 2024Quote: ... and digested with NheI-HF and SphI-HF (NEB). Adding mBCs to the reporter gene allows us to test larger libraries by amplifying sublibraries with different primer sets and cloning each sublibrary in parallel with a unique mBC ...
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bioRxiv - Genomics 2024Quote: ... Ligation products were transformed into either 5-alpha or 10-beta electrocompetent cells (NEB) and grown in liquid LB-Amp cultures ...
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bioRxiv - Genomics 2024Quote: ... Oligos were amplified through multiple PCR reactions (New England Biolabs [NEB] Q5 High-Fidelity 2X Master Mix ...
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bioRxiv - Genomics 2024Quote: ... and digested using DpnII (NEB) overnight at 37°C ...