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Citations for New England Biolabs :
201 - 250 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2024Quote: ... and in the YB_TATA minimal promoter using SpeI-HF (NEB). The natural TF binding site array inserts were designed to match these overhangs and replace the part of the YB_TATA minimal promoter that was removed when linearizing the backbone ...
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bioRxiv - Synthetic Biology 2024Quote: ... 1 µL T4 Ligase (400 U/µL; NEB), 1 µL backbone (typically around 50 ng) ...
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bioRxiv - Synthetic Biology 2024Quote: ... 0.5 µL BsaI-HF (20 U/µL; NEB), 0.5 µL T4 Ligase (400 U/µL ...
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bioRxiv - Synthetic Biology 2024Quote: ... 0.5 µL T4 Ligase (400 U/µL; NEB), 10 fmol of vector ...
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bioRxiv - Synthetic Biology 2024Quote: ... integration vector backbones were transformed into chemically competent NEB Stable Escherichia coli (NEB #C3040H), and cells were grown at 30 °C ...
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bioRxiv - Synthetic Biology 2024Quote: ... PCR amplification of the library region was performed with Q5 High Fidelity Polymerase (NEB) using primers containing Illumina partial adapters ...
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bioRxiv - Systems Biology 2024Quote: ... and 1 µl of Klenow large fragment DNA polymerase (New England Biolabs M0210) were then added ...
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bioRxiv - Systems Biology 2024Quote: ... the cDNA was diluted to 0.05 µg/ml and PCR-amplified using Phusion DNA polymerase (New England Biolabs M0530) with 0.5 mM dNTPs ...
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bioRxiv - Systems Biology 2024Quote: ... 2 µl of NEBuffer 2 (New England Biolabs B7002) and 1 µl of Klenow large fragment DNA polymerase (New England Biolabs M0210 ...
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bioRxiv - Systems Biology 2024Quote: ... 3 µl of 100 µM barcode-linked oligo-dT primer was added to each well with 5 µl of 5x ProtoScript RT buffer (New England Biolabs M0368). The plate was incubated at 94°C for 2 min and immediately cooled on ice for at least 5 min ...
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bioRxiv - Synthetic Biology 2024Quote: ... coli DH10B (New England Biolabs) was used for all routine cloning and biosensor characterization ...
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bioRxiv - Systems Biology 2024Quote: ... 5µl of RNA (roughly corresponding to 1µg) was reverse transcribed to cDNA using the LunaScript RT SuperMix kit - dye based qPCR detection (NEB) following manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2024Quote: ... Purification of PCR products for downstream applications was accomplished using either phenol/chloroform ethanol precipitation or the Monarch® PCR & DNA Cleanup Kit (New England Biolabs) according to the manufacturer’s instructions.
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bioRxiv - Synthetic Biology 2024Quote: ... The HDRT integration site was PCR-amplified from genomic DNA using Q5 High-Fidelity Polymerase (NEB), with an expected amplicon size of ∼1kb for non-edited B2M alleles and ∼2kb for alleles with HDRT integration ...
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bioRxiv - Synthetic Biology 2024Quote: ... nucleic acids were digested by addition of 10 µL of DNaseI (2000 units/mL, New England Biolabs) and CaCl2 to a final concentration of 10 mM ...
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bioRxiv - Synthetic Biology 2024Quote: ... coli (New England Biolabs, C3020K) according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2024Quote: ... PCRs were carried out using Q5 High-Fidelity DNA Polymerase (New England Biolabs). Following DpnI digest and PCR purification ...
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bioRxiv - Synthetic Biology 2024Quote: ... IVT-produced RNA samples were purified using a Monarch® RNA Cleanup Kit (New England Biolabs). ssRNA during RNA extractions was degraded with RNase T1 (Thermo Scientific) ...
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bioRxiv - Synthetic Biology 2024Quote: ... Ligations were performed using Quick Ligation™ Kit (New England Biolabs). HPLC and LC-MS mobile phases were prepared using ≥99.0% (GC ...
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bioRxiv - Synthetic Biology 2024Quote: ... and purified using Monarch® PCR & DNA Cleanup Kit (New England Biolabs). Gel fragments were extracted using GeneJet Gel extraction kit (Thermo Fisher Scientific) ...
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bioRxiv - Synthetic Biology 2024Quote: ... All constructs were assembled into a novel lentiviral destination backbone50,55 via a PaqCI reaction following manufacturer instructions (New England Biolabs #R0745S). The Gal4-NGN2 plasmid was constructed using Gibson Assembly with In-Fusion® Snap Assembly Master Mix (Takara Bio #638947 ...
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bioRxiv - Synthetic Biology 2024Quote: ... In vitro transcription (IVT) eactions were performed with HiScribe™ T7 High Yield RNA Synthesis Kit (New England Biolabs). Template DNA was degraded with TURBO DNase (Thermo Fisher Scientific) ...
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bioRxiv - Synthetic Biology 2024Quote: All replicative plasmid backbones were constructed using the Golden Gate Assembly strategy with the type II restriction enzyme Esp3I (NEB) according to the manufacturer’s protocol (Table 1) ...
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bioRxiv - Synthetic Biology 2024Quote: ... Amplicons are treated with 15U of Dpn1 (NEB) for 2.5 hours at 37°C followed by 20 minutes at 80°C ...
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bioRxiv - Synthetic Biology 2024Quote: ... DH10B (NEB) electrocompetent cells are transformed with 2μL of diluted isothermal assembly reaction ...
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bioRxiv - Synthetic Biology 2024Quote: ... Isothermal assembly followed Gibson Assembly protocols (NEB), but contained 100 mM Tris-HCl pH 7.5 ...
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bioRxiv - Synthetic Biology 2024Quote: ... and backbone were assembled in a single Golden Gate reaction (NEB) as described above.
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bioRxiv - Synthetic Biology 2024Quote: ... and TtgR_ColE1_SPS_V5 backbone were assembled using Golden Gate assembly (NEB). The libraries with approximately 15 barcodes per variant ...
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bioRxiv - Synthetic Biology 2024Quote: ... using the NEBNext Ultra II Q5 Master Mix (NEB, #M0544), primers (0.5 μM each ...
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bioRxiv - Synthetic Biology 2024Quote: ... The spacer was inserted into the backbone using Golden Gate (NEB) following standard protocols.
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bioRxiv - Synthetic Biology 2024Quote: ... 1ng of RNA was added to Luna Universal One-Step qRT-PCR mix (NEB) containing 4μmol of each primer on ice ...
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bioRxiv - Synthetic Biology 2024Quote: ... which were performed using OneTaq® Polymerase (NEB). For plasmid assembly ...
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bioRxiv - Synthetic Biology 2024Quote: Plasmid libraries are generated using Golden Gate Assembly Kits (NEB, BsaI-HFv2). The reactions undergo a cycling protocol of 30 alternating 5-minute 37°C and 16°C cycles followed by a final 60°C 5-minute hold ...
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bioRxiv - Synthetic Biology 2024Quote: ... The purified RNA was digested using 4U DnaseI (NEB) in a 50μL reaction incubated at 37°C for 30 minutes ...
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bioRxiv - Synthetic Biology 2024Quote: ... along with 8 units of RNAse inhibitor (NEB), and 10 µm malachite green oxalate added to each reaction ...
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bioRxiv - Synthetic Biology 2024Quote: ... Purified plasmids were first amplified and linearized using Q5 Hot Start High-Fidelity DNA Polymerase (NEB); forward and reverse primers are listed in Table S5 ...
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bioRxiv - Synthetic Biology 2024Quote: Plasmid cloning was performed primarily using standard PCR and restriction enzyme cloning with Phusion DNA Polymerase (NEB #M0530L), restriction enzymes (NEB) ...
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bioRxiv - Synthetic Biology 2024Quote: ... 1 μL of diluted GA reaction was then used in a 50 μL PCR reaction using Q5 Hot Start High-Fidelity DNA Polymerase (New England Biolabs).
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bioRxiv - Synthetic Biology 2024Quote: Using the HiScribe T7 High Yield RNA Synthesis Kit (NEB) 1 µg of the amplified and linearized DNA construct was transcribed in an 80 µL reaction volume and incubated overnight at 37 °C ...
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bioRxiv - Systems Biology 2024Quote: ... Ligations used T4 DNA ligase (NEB M0202) and fragment assembly used NEBuilder Hifi (NEB E2621) ...
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bioRxiv - Synthetic Biology 2024Quote: ... The tDNA was generated in a subsequent PCR reaction (Q5 High-Fidelity DNA Polymerase, NEB) and purified using the E.Z.N.A Cycle Pure Kit (Omega Bio-Tek) ...
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bioRxiv - Synthetic Biology 2024Quote: ... were used with 1 µL T4 DNA Ligase Reaction Buffer (NEB, 10x). We found that using these enzymes resulted in higher efficiency and fidelity for the cloning of mismatched gRNA libraries ...
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bioRxiv - Synthetic Biology 2024Quote: ... The PCR reaction was set up with 1 µL of the supernatant in a 12.5 µL reaction with Taq polymerase (NEB), according to manufacturer’s instruction.
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bioRxiv - Synthetic Biology 2024Quote: ... then replaced with T7 RNAP mix (New England Biolabs E2040S). The sample was incubated at 37 C overnight in a humidified tupperware.
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bioRxiv - Synthetic Biology 2024Quote: ... Golden Gate Assembly reactions were performed in a volume of 10 µL with 0.5 µL of either Esp3I (10,000 U/mL, NEB) or BsaI (10,000 U/mL Thermo Scientific) ...
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bioRxiv - Synthetic Biology 2024Quote: ... and 0.5 µL T4 DNA Ligase (NEB, 400,000 U/mL) were used with 1 µL T4 DNA Ligase Reaction Buffer (NEB ...
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bioRxiv - Synthetic Biology 2024Quote: ... Assembly of the final CRISPRi plasmid was done by combining the libraries of the gRNA position vectors with the pST_301 plasmid in a Golde Gate reaction as described above but with Esp3I (NEB, 10,000 U/mL) instead of BsaI ...
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bioRxiv - Synthetic Biology 2024Quote: ... using NEBNext Ultra II End repair/dA-tailing Module (New England Biolabs, USA) and NEB Blunt/TA Ligase Master Mix (New England Biolabs ...
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bioRxiv - Synthetic Biology 2024Quote: ... and both homology arms were ordered as gBlocks from Integrated DNA Technologies (IDT) and assembled (NEBuilder® HiFi DNA Assembly Master Mix (New England Biolabs (NEB)) ...
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bioRxiv - Synthetic Biology 2024Quote: ... and NEB Blunt/TA Ligase Master Mix (New England Biolabs, USA) respectively ...