Labshake search
Citations for New England Biolabs :
351 - 400 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Plant Biology 2024Quote: ... the full-length coding regions of TAL1 and TAC3 ligated into SpeI- and SmaI-digested pFGFP (R3133, R0141, New England BioLabs, Ltd., USA) using the In-Fusion method ...
-
bioRxiv - Plant Biology 2024Quote: ... the respective promoter was isolated via PCR using Phusion High Fidelity Enzyme (New England Biolabs, no. M0530L) and inserted upstream of GUS gene of pCambia1301 vectors ...
-
bioRxiv - Plant Biology 2024Quote: ... One µL of the end- prepped DNA was amplicons were barcoded with 1 µL of Nanopore Native Barcode using 5 µL Blunt/TA ligase master mix (NEB) in total reaction volume of 10 µL for 20 min at 20 °C ...
-
bioRxiv - Plant Biology 2024Quote: ... 0.25 µL of Q5 High-Fidelity DNA Polymerase (NEB), 14.75 uL of Nuclease Free Water (NFW ...
-
bioRxiv - Plant Biology 2024Quote: ... the full-length coding regions of TAL1 and ClHDA9 ligated into SmaI- and BamHI-HF-digested pDT1H-4MYC (R0141, R3136, New England BioLabs, Ltd., USA) using the In-Fusion method53 ...
-
bioRxiv - Plant Biology 2024Quote: ... The sequencing libraries were prepared by PCR amplification using the NEBNext PCR master mix (New England Biolabs, no. M0541S). The amplified libraries were purified with AMPure beads (Beckman Coulter ...
-
bioRxiv - Plant Biology 2024Quote: ... Sequencing libraries were generated using the NEBNext® Multiplex Small RNA Library Prep Set for Illumina (E7330S, New England BioLabs, Ltd., USA) following manufacturer’s recommendations and index codes were added to attribute sequences to each sample ...
-
bioRxiv - Plant Biology 2024Quote: ... Sequencing libraries were generated using the NEBNext® Ultra™ RNA Library Prep Kit for Illumina® (E7770, New England BioLabs, Ltd., USA) by the Beijing Allwegene Technology Company Limited (Beijing ...
-
bioRxiv - Plant Biology 2024Quote: ... 6 µg of the commercial EngenCas9 (NEB) with 2 µg of the freshly synthetized SgRNA1 obtained with the HiScribe™ T7 High Yield RNA Synthesis Kit (NEB) ...
-
bioRxiv - Plant Biology 2024Quote: ... The RNP complex was obtained by incubating for 30 min at 37°C in 15 µL of EngesCas9 specific buffer (NEB), 6 µg of the commercial EngenCas9 (NEB ...
-
Spray-induced gene silencing (SIGS) as a tool for the management of Pine Pitch Canker forest diseasebioRxiv - Plant Biology 2024Quote: ... 2018).Plasmid and inserts were digested with KpnI and BglII or SacII restriction enzymes (New England Biolabs, Ipswich, MA, USA) following manufacturer instructions ...
-
bioRxiv - Plant Biology 2024Quote: ... with 2 µg of the freshly synthetized SgRNA1 obtained with the HiScribe™ T7 High Yield RNA Synthesis Kit (NEB). DNA repair templates containing the fenhexamid resistance cassette surrounded by 60 bp of the target gene for HR were obtained by PCR ...
-
bioRxiv - Plant Biology 2024Quote: ... 0.02 U/µl of the Q5 High Fidelity DNA polymerase (New England BioLabs) and 1 µl of the template ...
-
bioRxiv - Plant Biology 2024Quote: ... the pooled amplicons were initially repaired using the Ultra II End Repair / dA-tailing Module (NEB, USA). Subsequently ...
-
bioRxiv - Synthetic Biology 2024Quote: ... 1 μL factor mix (NEB), 250 nM pre-incubated 50S ribosomal subunits ...
-
bioRxiv - Synthetic Biology 2024Quote: ... 3.2 μL solution A (NEB), 1 μL factor mix (NEB) ...
-
bioRxiv - Synthetic Biology 2024Quote: ... an in vitro translation mixture was assembled using the ΔRibosome PURExpress kit (NEB): 3.2 μL solution A (NEB) ...
-
bioRxiv - Synthetic Biology 2024Quote: ... pLK35 plasmids were transformed into NEB Express Iq cells (NEB) which are a bL21 derivative that constitutively expresses the lac repressor ...
-
bioRxiv - Synthetic Biology 2024Quote: After transformation into chemically competent BL21(DE3) cells (NEB), colonies were picked into deep-well plates (Greiner ...
-
bioRxiv - Plant Biology 2024Quote: ... Adapter ligation and library preparation were carried out using NEBNext Ultra II DNA Library Prep Kit for Illumina (NEB), and adapter ligation was checked using qPCR ...
-
bioRxiv - Biochemistry 2024Quote: ... PCR was performed using the Q5 high-fidelity polymerase (New England Biolabs). After each PCR step ...
-
bioRxiv - Plant Biology 2024Quote: ... barcodes were ligated using the NEB Blunt/TA Ligase Master Mix (NEB, USA). The entire sequencing procedure was performed on a MinION Mk1B device with a flowcell R9.4.1 ...
-
bioRxiv - Plant Biology 2024Quote: ... The RNA was ethanol precipitated and phosphorylated with T4 polynucleotide kinase (New England Biolabs) prior to library construction using the NEXTFLEX Small RNA-Seq Kit v4 (Perkin Elmer) ...
-
bioRxiv - Plant Biology 2024Quote: ... An aliquot containing 10 mg of protein was then subjected to lambda protein phosphatase reaction mix following the manufacturer’s instructions (New England Biolabs, US) for 1 hour at 30°C ...
-
bioRxiv - Biochemistry 2024Quote: ... Purified products were again transformed into either NEB 10-beta (New England Biolabs, Ipswich, MA) or SIG10 (Sigma-Aldrich ...
-
bioRxiv - Biochemistry 2024Quote: ... The resulting plasmid products were transformed into NEB 10-beta electrocompetent cells (New England Biolabs, Ipswich, MA) and purified using a ZymoPURE Midiprep Kit (Zymo Research ...
-
bioRxiv - Biochemistry 2024Quote: ... Nicking mutagenesis plasmid products were transformed into NEB 10-beta electrocompetent cells (New England Biolabs, Ipswich, MA) and purified using a ZymoPURE Midiprep Kit (Zymo Research ...
-
bioRxiv - Biochemistry 2024Quote: ... these libraries were then bottlenecked through an additional transformation in NEB Turbo electrocompetent cells (New England Biolabs, Ipswich, MA), and were again purified using a ZymoPURE Midiprep Kit (Zymo Research ...
-
bioRxiv - Plant Biology 2024Quote: ... Libraries were selected for cDNA target fragments of 300 bp on 2% Low Range Ultra Agarose followed by PCR amplified using Phusion DNA polymerase (NEB) for 15 PCR cycles ...
-
bioRxiv - Neuroscience 2024Quote: ... sgRNAs were mixed with Cas9 protein (M0646M NEB, or B25641 invitrogen) and coinjected into one-cell stage embryos using a microinjector (Eppendorf) ...
-
bioRxiv - Plant Biology 2024Quote: ... After DNase I treatment (New England Biolabs, Ipswich, MA, USA), 10 µg of RNA were further cleaned up by RNA Clean & Concentrator-5 (Zymo Research ...
-
bioRxiv - Plant Biology 2024Quote: Arabidopsis thaliana RNA sequencing libraries were prepared with Illumina-compatible NEBNext® Ultra™ II Directional RNA Library Preparation Kit (New England BioLabs, MA, USA). Five hundred ng of total RNA was taken for mRNA isolation ...
-
bioRxiv - Plant Biology 2024Quote: ... Nucleotide changes leading to amino acid substitutions for Pic3 and Pic12 were first created in pENTR/D-TOPO (which contained wild-type Pic3 or Pic12) vectors by using the Q5 site-directed mutagenesis kit (New England Biolabs) with specific primers (Supplemental Table S4) ...
-
bioRxiv - Plant Biology 2024Quote: ... ndhF amplicons were amplified with the Q5® polymerase (NEB) from all samples ...
-
bioRxiv - Plant Biology 2024Quote: 6X loading buffer (New England Biolabs, Ipswich, MA, USA)
-
bioRxiv - Plant Biology 2024Quote: ... the RNA was processed with the NEBNext Ultra II RNA Library Prep Kit from Illumina (NEB; E7770L). The libraries were then sequenced on an Illumina NextSeq1000 system and analyzed on the Galaxy platform (59) ...
-
bioRxiv - Plant Biology 2024Quote: ... 2µl of 6X loading buffer (New England Biolabs, Ipswich, MA) was added to each reaction and then loaded onto a 1% agarose/TBE gel along with 5 µl each of 100 bp Plus and 1 kb Plus DNA ladders (Goldbio ...
-
bioRxiv - Plant Biology 2024Quote: ... mRNA was isolated using NEBNext Poly(A) mRNA Magnetic Isolation Module (NEB). Libraries were prepared using NEBNext Ultra II RNA Library Prep Kit for Illumina (NEB ...
-
bioRxiv - Plant Biology 2024Quote: ... RT-qPCR was performed in technical triplicates using the Luna® Universal qPCR Master Mix (NEB) in a CFX96 Touch™ Real-Time PCR Detection System (Bio-Rad ...
-
bioRxiv - Plant Biology 2024Quote: ... DNA contamination was removed by 2U DNaseI (NEB; M0303S) then purified with the RNA clean and concentrator 5 kit (ZYMO ...
-
bioRxiv - Plant Biology 2024Quote: ... RNA samples were digested with DNase I (NEB) to remove genomic DNA ...
-
bioRxiv - Plant Biology 2024Quote: ... followed by purification with the Monarch RNA Clean Up Kit (NEB). RNA (1 µg per sample ...
-
bioRxiv - Plant Biology 2024Quote: ... Amplification specificity was assessed by agarose gel electrophoresis and amplicons were subsequently purified with the Monarch PCR & DNA Clean Up Kit (NEB). Resulting DNA concentrations were spectrophotometrically measured by NanoDrop ...
-
bioRxiv - Plant Biology 2024Quote: ... PCR fragments used for cloning were obtained using Q5 high-fidelity DNA polymerase (New England Biolabs, Frankfurt, Germany). All PCR-based constructs were sequenced ...
-
bioRxiv - Synthetic Biology 2024Quote: ... The resulting aqueous solution was concentrated using a spin column (Monarch, NEB) following the manufacturer’s instructions ...
-
bioRxiv - Bioengineering 2024Quote: ... Synthetic RNA was generated through in vitro transcription of gene fragments using HiScribe T7 High Yield RNA Synthesis Kit (New England Biolabs, Ipswich, MA, USA) and quantified via qPCR standard curve.
-
bioRxiv - Molecular Biology 2024Quote: ... and 10 pmol of each used in a 4-fragment Gibson assembly reaction (NEB) to generate TbGT8 5’_BSDr_TbGT8 3’_pUC19 ...
-
bioRxiv - Molecular Biology 2024Quote: Plasmid DNA was purified from Escherichia coli DH5α competent cells (New England Biolabs) using a Qiagen Miniprep kit ...
-
bioRxiv - Molecular Biology 2024Quote: ... ∼500 bp 5’ and 3’ flanking regions of Tb427.10.12290 (NEB) with primers SMD400/1 and SMD404/5 respectively using (Lister strain 427 ...
-
bioRxiv - Molecular Biology 2024Quote: ... the bound TfR was digested on beads with EndoH according to the manufacturer’s recommendations (New England Biolabs). Eluates were analysed by lectin or Western blotting as described above.