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Citations for New England Biolabs :
451 - 500 of 10000+ citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... Base editing sgRNA directed against the SF3B1 K700 locus was designed using BE-Designer28 and cloned into pLKO.5 Puro-2A-GFP between BsmBI (New England Biolabs) cut sites ...
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bioRxiv - Cancer Biology 2024Quote: ... the hU6-pegRNA2-polyT cassette was amplified from the pU6-pegRNA-gg-acceptor backbone and ligated into ngRNA1 pLKO.5 Puro-2A-RFP between EcoRI and XhoI (New England Biolabs) to create pLKO.5-K700E-ng1+pg2-Puro-2A-RFP ...
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bioRxiv - Bioengineering 2024Quote: ... Cleaved products were incubated with proteinase K (NEB, Ipswich, MA), A-tailed ...
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bioRxiv - Bioengineering 2024Quote: ... DNA circularization was performed using T4 DNA ligase (NEB, Ipswich, MA), followed by treatment with a mixture of exonucleases to remove residual linear DNA ...
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bioRxiv - Bioengineering 2024Quote: ... USER enzyme (NEB, Ipswich, MA) and T4 polyncucleotide was then used to treat the gap-repaired DNA ...
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bioRxiv - Bioengineering 2024Quote: ... PCRs were carried out using the Q5 Hot Start 2x Master Mix (NEB, Ipswich, MA) according to the manufacturer’s protocol for 30 cycles ...
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bioRxiv - Bioengineering 2024Quote: ... ligated with a hairpin adapter (NEB, Ipswich, MA), and treated with USER enzyme (NEB ...
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bioRxiv - Bioengineering 2024Quote: ... single indices for demultiplexing were added using the NEBNext Multiplex Oligos for Illumina (NEB, E7335S, E7500S, or E7710S) in 6-cycle PCR ...
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bioRxiv - Bioengineering 2024Quote: High Molecular Weight (HMW) DNA was obtained using the Monarch® HMW DNA extraction kit for Tissue (T3060L, New England Biolabs), as previously described (Low et al ...
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bioRxiv - Bioengineering 2024Quote: ... The RNA template of the SARS-CoV-2 N gene was synthesised using in-vitro transcription (Hiscribe, NEB, US) and a template plasmid (Molecular Diagnostics Collection ...
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bioRxiv - Bioengineering 2024Quote: ... Typhi (the bacterial pathogen that causes typhoid fever) using 2X Warmstart colorimetric LAMP (M1800, NEB, US). The RNA template of the SARS-CoV-2 N gene was synthesised using in-vitro transcription (Hiscribe ...
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bioRxiv - Biophysics 2024Quote: M2R constructs were dephosphorylated through a 30-minute room temperature treatment with 200 units of lambda protein phosphatase (NEB), 10 units of calf intestinal phosphatase (NEB) ...
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bioRxiv - Biophysics 2024Quote: ... 10 units of calf intestinal phosphatase (NEB), 5 units of antarctic phosphatase (NEB) ...
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bioRxiv - Bioengineering 2024Quote: ... gDNA extraction using Monarch® Genomic DNA Purification Kit (New England Biolabs), and histology sectioning.
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bioRxiv - Bioengineering 2024Quote: ... Then it was incubated with 20ME (Molar equivalent) of BG-GLA-NHS (NEB) for 30 minutes at room temperature ...
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bioRxiv - Bioengineering 2024Quote: ... The TSA1 promoter was amplified by PCR from genomic DNA obtained from the X-33 strain using Q5 polymerase (New England Biolabs) and primers TSA1_BstBI_Fw and TSA1-GFP_Rv_KpnI ...
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bioRxiv - Biophysics 2024Quote: ... and then transformed BL21(DE3) Escherichia coli (New England BioLabs) with this plasmid ...
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bioRxiv - Biophysics 2024Quote: ... 5 units of antarctic phosphatase (NEB), and 1 mM manganese chloride ...
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bioRxiv - Cancer Biology 2024Quote: ... that has been previously digested with XbaI (New England Biolabs, Bioconcept) and XmaI ...
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bioRxiv - Bioengineering 2024Quote: ... Synthesized mRNA was purified using the Monarch RNA cleanup kit (New England Biolabs) and stored at −80°C ...
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bioRxiv - Bioengineering 2024Quote: ... coli strains used in this study were DH5α (New England Biolabs) for cloning ...
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bioRxiv - Cancer Biology 2024Quote: ... and treated with 800 U of Lambda Phosphates (New England Biolabs) for 30 min at 30°C ...
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bioRxiv - Bioengineering 2024Quote: All polymerase chain reaction (PCR) amplification steps were performed using Phusion DNA polymerase (NEB, Whitby, Ontario, Canada). Primers used for PCR amplification were ordered from Integrated DNA Technologies (IDT ...
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bioRxiv - Bioengineering 2024Quote: ... coli DH5α (Matthew Scott Lab, University of Waterloo) and NEB10-β (New England Biolabs (NEB), Whitby ...
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bioRxiv - Bioengineering 2024Quote: ... the DNA templates were linearized by digestion with HindIII and ScaI (NEB) and purified by phenol-chloroform extraction ...
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bioRxiv - Neuroscience 2024Quote: ... (R0701S and R0712S, New England Biolabs) and then combined with the homology arms in a ligation reaction (M0202S ...
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bioRxiv - Neuroscience 2024Quote: ... The template for the sst1.1 probe was a cDNA clone in a pSPORT1 vector (Argenton et al., 1999) linearized by digestion with SalI (R3138L, New England Biolabs).
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bioRxiv - Molecular Biology 2024Quote: Restriction enzymes: all restriction enzymes acquired from NEB: BbsI (R0539S) ...
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bioRxiv - Molecular Biology 2024Quote: ... and indexed with NEBNext® Multiplex Oligos for Illumina (catalog no. E6440S, NEB), according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2024Quote: ... libraries were constructed with the NEBNext® Ultra™ II Directional RNA Library Prep Kit for Illumina (catalog no. E7760L, NEB) and indexed with NEBNext® Multiplex Oligos for Illumina (catalog no ...
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bioRxiv - Molecular Biology 2024Quote: ... The crosslink bearing oligo and an uncrosslinked control was then phosphorylated by T4 polynucleotide kinase (New England Biolabs M0201S).
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bioRxiv - Molecular Biology 2024Quote: ... Enriched DNA was subjected to library construction using the NEBNext® Ultra™ II DNA Library Prep Kit for Illumina (catalog no. E7645L, NEB), with the adapter ligation step performed at 50°C instead of 65°C ...
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bioRxiv - Molecular Biology 2024Quote: ... and a 50 pm RNA probe was incubated with 20U T4 Polynucleotide kinase (PNK) (NEB, catalogue # M0201S), 1X T4 PNK buffer and 30 µCi P32ATP at 37°C for 30minutes (reaction volume 50µl ...
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bioRxiv - Molecular Biology 2024Quote: ... Small RNA libraries were prepared using the NEBNext Small RNA Library Prep kit (NEB), with some modifications ...
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bioRxiv - Molecular Biology 2024Quote: ... radiolabelled using Reaction Buffer (10mM Tris-HCl, 5mM MgCl2, 7.5mM DTT [pH 7.5]) and Exo-Klenow DNA polymerase (NEB, catalogue #M0212S); and Stop Solution (10mM EDTA ...
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bioRxiv - Molecular Biology 2024Quote: ... PCR products were digested by ExoI (NEB) to remove excess of oligos and purified using Qiagen PCR purification kit ...
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bioRxiv - Molecular Biology 2024Quote: ... The phosphorylated DNA strand was then specifically digested with lambda exonuclease (NEB); the ssDNA strands obtained were checked on 0.8% agarose gel ...
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bioRxiv - Molecular Biology 2024Quote: ... and 10 units of PNK (NEB) in a total volume of 25µl PNK buffer 1X ...
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bioRxiv - Molecular Biology 2024Quote: ... and 2) library for miRNAs detection utilizing NEBNext® Small RNA Library Prep Set for Illumina (New England Biolabs (UK) Ltd ...
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bioRxiv - Molecular Biology 2024Quote: ... Two microliters of digested samples were used to run the reaction of PCR with Taq 2X Master Mix (New England Biolabs (UK) Ltd ...
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bioRxiv - Cancer Biology 2024Quote: ... T4 DNA Ligase (New England BioLabs #M0202L) and Ligase Buffer (New England BioLabs #B0202S) ...
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bioRxiv - Cancer Biology 2024Quote: ... Real-time PCR reactions were prepared using the Luna® Universal qPCR Master Mix (NEB). Reactions were run and data analyzed using the QuantStudioTM 7 Flex Real-time PCR system (Applied Biosystems) ...
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bioRxiv - Cancer Biology 2024Quote: ... The transcription products were purified with Monarch RNA Cleanup Kit (NEB) followed by polyadenylation using Poly(U ...
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bioRxiv - Cancer Biology 2024Quote: ... and purification with Monarch RNA Cleanup Kit (NEB). RNAs were then used for MeRIP assays.
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bioRxiv - Cancer Biology 2024Quote: ... PCR-amplified DNA templates encoding 5’UTR of ATF4 mRNA were used for in vitro transcription of ATF4 reporter mRNA using HiScribe T7 High Yield RNA Synthesis Kit (NEB) according to the manufacturer’s protocols ...
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bioRxiv - Cancer Biology 2024Quote: ... followed by polyadenylation using Poly(U) Polymerase (NEB) with Bio-16-UTP (Invitrogen ...
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bioRxiv - Cancer Biology 2024Quote: ... and the chromatin was digested using 200 units of HindIII (NEB) at 37 °C overnight and then the digestion was stopped with 1.6% SDS at 65 °C for 20 min ...
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bioRxiv - Cancer Biology 2024Quote: ... Digested DNA fragments were ligated using T4 DNA ligase (NEB) for 4 h at 16 °C ...
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bioRxiv - Cell Biology 2024Quote: ... RNA sequencing library preparation used the NEBNext Ultra RNA Library Prep Kit for Illumina followed by manufacturer’s instructions (NEB, Ipswich, MA, USA). Briefly ...
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bioRxiv - Cell Biology 2024Quote: ... Protein A magnetic beads (New England Biolabs) were saturated with rabbit-anti-GFP ...