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Citations for New England Biolabs :
451 - 500 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2024Quote: ... according to manufacturer’s instructions) and cloned into the SacI (NEB) digested pAbAi plasmid (Takarabio) ...
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bioRxiv - Pathology 2024Quote: ... 5 min ice) and plasmids were extracted using the Monarch Plasmid Miniprep kit (NEB) following manufacturer’s instructions.
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bioRxiv - Pathology 2024Quote: ... schachtii cDNA (Q5 polymerase (NEB)) and cloned into the PCR amplified pDEST22 plasmid (Invitrogen) ...
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bioRxiv - Genomics 2024Quote: ... multiplexed stranded cDNA sequencing libraries were prepared using the NEBNext Ultra Directional RNA Library Prep Kit for Illumina (New England BioLabs, Ipswich, MA, USA) using poly(A ...
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bioRxiv - Immunology 2024Quote: ... Oxford Genomics Centre prepared bulk RNA libraries using an NEBNext Single Cell/Low Input RNA Library Prep Kit for Illumina (New England Biolabs) and sequenced the samples at a depth of 25 million reads per sample on a NovaSeq6000 (Illumina) ...
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bioRxiv - Microbiology 2024Quote: Suitable primer pairs (Table S5) were designed utilizing the Q5 site-directed mutagenesis kit (New England Biolabs) to generate mutated amidases ...
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bioRxiv - Pathology 2024Quote: ... Prior to transformation the bait plasmids were linearised in the URA3 gene by PCR or restriction digest (BbsI/Esp3I/BstBI (NEB)) to allow integration into the genome ...
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bioRxiv - Pathology 2024Quote: ... schachtii gDNA (Q5 polymerase (NEB) according to manufacturer’s instructions ...
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bioRxiv - Pathology 2024Quote: ... The isolated RNA was then reverse transcribed into first-strand cDNA using LunaScript RT SuperMix (NEB) according to the manufacturer’s instructions ...
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bioRxiv - Pathology 2024Quote: ... qRT PCR was performed with the LUNA Universal qPCR Master Mix (NEB) following manufacturer’s instructions and 1 µl of cDNA ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 2.6 µL of 10% NP-40 (Cat.#B2704S; New England Biolabs), and 1.5 µL of PNGase F in a total reaction volume of 26.7 µL ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 40 – 50 µg of protein lysate from each sample was denatured in 1X Glycoprotein Denaturing Buffer (Cat.#B1704S; New England Biolabs) with 20 µL total volume at 100°C for 10 min ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... or PNGase F (Cat.#P0708L; New England Biolabs). For Endo Hf digestion ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 20 µL of denatured protein lysate was combined with 2.6 µL of 10X GlycoBuffer 2 (Cat.#B3704S; New England Biolabs), 2.6 µL of 10% NP-40 (Cat.#B2704S ...
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bioRxiv - Cancer Biology 2024Quote: ... Q5 High-Fidelity 2x Master Mix (M0494X, New England Biolabs) was used to amplify the U6-BC-crRNA region from 32 μg of genomic DNA in a total reaction volume of 800 μl per sample using unique dual-indexed primers as described22 ...
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bioRxiv - Pathology 2024Quote: ... The resulting eluent was further purified with amylose resin (New England BioLabs E8021S) which binds MBP and then washed with amylose wash buffer (50 mM HEPES ...
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bioRxiv - Cell Biology 2024Quote: ... RNA was digested with RNase H (NEB) and RNase A/T1 (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... using the NEBNext Poly(A) mRNA Magnetic Isolation Module and NEBNext Ultra II Directional RNA Library Prep Kit for Illumina (New England Biolabs (NEB), Ipswich ...
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bioRxiv - Cancer Biology 2024Quote: ... and poly(A) mRNA was isolated using the NEBNext Poly(A) mRNA Magnetic Isolation kit (NEB E7490L). The RNA library for direct RNA sequencing was prepared from ∼500 ng of poly-A RNA according to the Oxford Nanopore technology (ONT ...
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bioRxiv - Cell Biology 2024Quote: ... coli BL21 (DE3) (C2527I, New England Biolabs) and grown in 1 L of Terrific Broth (TB ...
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bioRxiv - Cell Biology 2024Quote: ... NEB)/EcoRI-HF (Cat #: R310S; NEB) digestion and subsequent ligation (Cat # ...
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bioRxiv - Cell Biology 2024Quote: ... was used to generate pcDNA3.1-ABDTS from pcDNA3.1 vector digested with EcoRI-HF (Cat #: R310S; NEB)/NotI-HF (Cat # ...
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bioRxiv - Cell Biology 2024Quote: ... digestion and subsequent ligation (Cat #: M0202S; NEB). All newly generated constructs were verified by DNA sequencing (GENEWIZ from Azenta).
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bioRxiv - Cell Biology 2024Quote: ... Gibson Assembly (with Gibson Assembly Master Mix, E2611S; NEB, Ipswich, MA) was used to generate pcDNA3.1-ABDTS from pcDNA3.1 vector digested with EcoRI-HF (Cat # ...
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bioRxiv - Cell Biology 2024Quote: ... pcDNA3.1-ABDTL was generated via PCR from pcDNA3.1-ABDTS and inserted into pcDNA3.1 via BamHI-HF (Cat #: R3136S; NEB)/EcoRI-HF (Cat # ...
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bioRxiv - Cell Biology 2024Quote: ... NEB)/NotI-HF (Cat #: R3189S; NEB,) and the following three fragments containing complementary regions ...
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bioRxiv - Microbiology 2024Quote: ... GM16 genomic DNA using primers SA-Reg FWD and SA-Reg REV (Table 4) and polymerase Q5 (New England Biolabs, Massachusetts, USA). The destination plasmid pGW44 [33] was linearized using primers pGW44 FWD and pGW44 REV ...
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bioRxiv - Microbiology 2024Quote: ... The SA pathway was then introduced into pGW44 using the Gibson Assembly Cloning Kit (New England BioLabs), creating plasmid pJM455 ...
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bioRxiv - Microbiology 2024Quote: ... The vector and amplified arms were digested using the PmeI cut site (NEB). The arms and vector were ligated using the Gibson Assembly cloning kit (NEB) ...
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bioRxiv - Microbiology 2024Quote: ... The arms and vector were ligated using the Gibson Assembly cloning kit (NEB), thus generating pMSR::630Δerm_relQ_arms ...
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bioRxiv - Microbiology 2024Quote: ... for subsequent purification with DNaseI (NEB) treatment ...
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bioRxiv - Cancer Biology 2024Quote: ... Briefly the plasmid was digested with Esp3I (NEB#R0734) at 37°c overnight ...
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bioRxiv - Cancer Biology 2024Quote: ... Single stranded oligo pools were ordered from IDT (oPools™ Oligo Pools) and built into the digested PCC_01 vector using NEBuilder HiFi DNA Assembly Master Mix (E2621L, New England Biolabs) according to the manufacturer’s instruction ...
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bioRxiv - Microbiology 2024Quote: ... qRT-PCR reactions were conducted using the Luna Universal qPCR Master Mix (New England BioLabs) according to manufacturer instructions ...
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bioRxiv - Microbiology 2024Quote: ... using the Taq DNA Polymerase (NEB) and primers that bind in the deletion cassette and outside of the homologous regions upstream and downstream (Table S1) ...
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bioRxiv - Microbiology 2024Quote: ... All PCR reactions were performed using Q5 High-Fidelity 2X Master Mix (NEB, Frankfurt, Germany). The PCR product was purified and sequencing was done using a gene specific primer (GSP3) ...
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bioRxiv - Microbiology 2024Quote: ... and the PNGase F assay (New England Biolabs) was completed following the manufacturer’s instructions using 15 µg of protein ...
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bioRxiv - Microbiology 2024Quote: ... with either RNase-free DNase I (NEB), 100 ng of RNase A ...
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bioRxiv - Microbiology 2024Quote: ... in vitro translation of the short-model mRNA was performed using a PURExpress® In VitroProtein Synthesis Kit (New England Biolabs, US). The reaction mixture (volume ...
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bioRxiv - Microbiology 2024Quote: ... Genomic libraries were prepared using the NEBNext Ultra II FS library preparation kit (New England Biolabs, USA) according to the manufacturer’s instructions for MiSeq (Illumina ...
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bioRxiv - Microbiology 2024Quote: ... (NEB). The p57mA3 plasmid was digested with MscI restriction enzyme and the following oligomers were used ...
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bioRxiv - Microbiology 2024Quote: The msdDNA cDNA was isolated from acrylamide gels and 100 ng was used to extend the 3’ end with dCTP or dGTP and terminal deoxynucleotidyl transferase (TdT) from NEB, according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... Spacers were introduced into pNA15 via Golden Gate cloning with BsaI-HFv2 (New England Biolabs Cat. #R3733S) and T7 DNA ligase (New England Biolabs Cat ...
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bioRxiv - Microbiology 2024Quote: ... 200 ng of nucleic acids from each complex were mixed in 1xDNase I buffer (NEB) with either RNase-free DNase I (NEB) ...
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bioRxiv - Microbiology 2024Quote: ... and T7 DNA ligase (New England Biolabs Cat. #M0318S), then electroporated into Endura Duo electrocompetent cells (Biosearch Technologies Cat ...
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bioRxiv - Molecular Biology 2024Quote: ... the lentiCRISPR-v2 vector underwent BsmBI-v2 (NEB, Cat# R0739L) digestion and Quick CIP (NEB ...
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bioRxiv - Molecular Biology 2024Quote: ... digestion and Quick CIP (NEB, Cat# M0525S) dephosphorylation ...
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bioRxiv - Immunology 2024Quote: ... with the exception of KLF2 which was performed using the Q5 High-Fidelity kit (NEB). For the 11q deletion variant (P3) ...
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bioRxiv - Immunology 2024Quote: Streptavidin magnetic beads (BioLabs, Cat. #S1420S) were washed twice with PBST and incubated with recombinant biotinylated spike proteins (spike ...
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bioRxiv - Microbiology 2024Quote: ... 5 μL of GC Enhancer (NEB), 5 μL of 5X buffer ...