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Citations for New England Biolabs :
551 - 600 of 10000+ citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2024Quote: ... then ligated to reporter gene inserts in multiple reactions (NEB T4 ligase). Ligation products were transformed into electrocompetent cells and grown in liquid culture from which we prepared plasmid DNA.
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bioRxiv - Genomics 2024Quote: ... followed by 5′ cap repair with RppH (NEB) and 5′ hydroxyl repair with PNK (NEB) ...
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bioRxiv - Developmental Biology 2024Quote: ... 0.01% Molecular Grade BSA (New England Biolabs), 146 mM NaCl (ThermoFisher Scientific) ...
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bioRxiv - Genomics 2024Quote: ... NEBNext Ultra II Directional RNA Library Prep kit with poly(A) mRNA isolation was used to construct RNA-seq libraries according to the manufacturer’s instructions (NEB). Sequencing reads were aligned to hg19 build of the human genome using HISAT2 (Kim et al. ...
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bioRxiv - Genomics 2024Quote: ... dCas9-VP16(10x) plasmid was linearized with restriction enzyme AflII (New England Biolabs R0520S). Successful integration of the dCas9-VP16(10x ...
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bioRxiv - Genomics 2024Quote: ... followed by 3′ adapter ligation using T4 RNA ligase (NEB). Biotinylated RNAs were enriched for a second time ...
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bioRxiv - Genomics 2024Quote: ... Oligos were amplified through multiple PCR reactions (New England Biolabs [NEB] Q5 High-Fidelity 2X Master Mix ...
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bioRxiv - Genomics 2024Quote: ... Plasmid pools were digested with SphI-HF and SpeI-HF and treated with Antarctic phosphatase or Quick CIP (NEB), then ligated to reporter gene inserts in multiple reactions (NEB T4 ligase) ...
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bioRxiv - Microbiology 2024Quote: ... and 750 bp upstream and 747 bp downstream were cloned using primers JCP343/344 into SmaI (NEB)-digested pTOX5 allelic exchange vector44 ...
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bioRxiv - Molecular Biology 2024Quote: ... NEBNext Small RNA Library Prep kit (NEB), RNA oligonucleotides (Horizon Discoveries) ...
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bioRxiv - Molecular Biology 2024Quote: ... Sequencing libraries were made using the Small RNA library preparation kit NEBNext (NEB) Small RNA library prep set according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... and T4 polynucleotide kinase (New England BioLabs (NEB)) ...
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bioRxiv - Cancer Biology 2024Quote: ... and T4 polynucleotide kinase (New England BioLabs (NEB)) ...
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bioRxiv - Cancer Biology 2024Quote: ... A single adenine base was added to fragment ends by Klenow fragment (3′ to 5′ exo minus; NEB), followed by ligation of Illumina adaptors (Quick ligase ...
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bioRxiv - Cancer Biology 2024Quote: ... followed by ligation of Illumina adaptors (Quick ligase, NEB). The adaptor-ligated DNA fragments were enriched by PCR using the Illumina Barcode primers and Phusion DNA polymerase (NEB) ...
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bioRxiv - Cancer Biology 2024Quote: ... The adaptor-ligated DNA fragments were enriched by PCR using the Illumina Barcode primers and Phusion DNA polymerase (NEB). PCR products were size-selected using 3% NuSieve agarose gels (Lonza ...
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bioRxiv - Cancer Biology 2024Quote: ... were incorporated into telomeres by 5U Taq DNA polymerase at 72°C for one hour in 1X Thermopol buffer (New England Biolabs). The nick-labeled sample was treated with proteinase K (QIAGEN ...
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bioRxiv - Biochemistry 2024Quote: ... and 4 units of Proteinase K (New England Biolabs).
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bioRxiv - Biochemistry 2024Quote: ... linearized with NdeI and XhoI (NEB), using HiFi DNA Assembly Master Mix (NEB ...
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bioRxiv - Biochemistry 2024Quote: ... Cloning reagents were purchased from New England BioLabs (NEB). Codon-optimized gene fragments were purchased from Twist Biosciences ...
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bioRxiv - Biochemistry 2024Quote: A Q5 Site-Directed Mutagenesis Kit (NEB, E0554S) was used to introduce mutations in amino acid residues predicted to play a role in stabilizing zinc ions ...
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bioRxiv - Biochemistry 2024Quote: ... using HiFi DNA Assembly Master Mix (NEB) following the manufacturer’s instructions (Supplementary Table 3) ...
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bioRxiv - Biochemistry 2024Quote: ... and tRNAGln with 5 nt-long 5’ leader and same 24 nt-long 3’ trailer as tRNATyr (5–tRNAGln–24) – were transcribed in reactions containing 1x T7 RNA polymerase reaction buffer (NEB), 0.001% (w/v ...
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bioRxiv - Biochemistry 2024Quote: ... coli PPIase (NEB), 5 U µL-1 T7 RNA polymerase (Thermo Fisher ...
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bioRxiv - Biochemistry 2024Quote: ... coli Shuffle T7 Express cells (C3029J, New England Biolabs) to aid disulfide bond formation ...
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bioRxiv - Biochemistry 2024Quote: ... library indexing was carried out with the NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs, Cat. E7645S) and sequenced on a NextSeq 1000 P2 cartridge (Illumina ...
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bioRxiv - Biochemistry 2024Quote: ... crosslinked RNA fragments were dephosphorylated at their 3’-ends using T4-PNK (NEB, M0201S) and ligated to a pre-adenylated 3’-adapter (L3-App ...
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bioRxiv - Biochemistry 2024Quote: The cloning of human wild type Mad2 and the mutant Mad2R133A by the USER® method (NEB) into the pRSFDuet-1 vector (71341-3 ...
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bioRxiv - Biochemistry 2024Quote: ... 2 µl T4-PNK (10 U/µl, NEB) and 9.5 µl H2OMQ were added ...
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bioRxiv - Biochemistry 2024Quote: ... 2 µl T4-PNK buffer (NEB), 2 µl T4-PNK (10 U/µl ...
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bioRxiv - Biochemistry 2024Quote: ... NEBuilder HiFi DNA Assembly Master Mix (NEB) was used for subcloning ...
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bioRxiv - Biochemistry 2024Quote: ... Methylated template DNA was digested using DpnI (NEB, Ipswich, MA, United States), before heat-shock transformation into chemically competent E ...
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bioRxiv - Biochemistry 2024Quote: ... All cloning was performed using Gibson assembly cloning (NEB). All cDNA constructs were confirmed by sequencing.
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bioRxiv - Biochemistry 2024Quote: ... and NEBStable (NEB) cells were used for molecular cloning ...
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bioRxiv - Biochemistry 2024Quote: All PCR was performed with Q5 High-Fidelity DNA Polymerase kit (New England Biolabs) according to manufacturer’s protocols ...
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bioRxiv - Biochemistry 2024Quote: ... we employed specific restriction enzymes (XhoI, NcoI, and NdeI from NEB) to cleave the plasmids at predetermined sites corresponding to the gene insertion ...
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bioRxiv - Biochemistry 2024Quote: ... coli BL-21 cells (New England Biolabs) were used for the transformation of pET-28b(+ ...
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bioRxiv - Bioengineering 2024Quote: ... dsDNA was mixed with lambda exonuclease (New England Biolabs, M0262) in the vendor supplied buffer ...
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bioRxiv - Biochemistry 2024Quote: ... The strep tags were removed by incubating the proteins with 64 units of Enterokinase light chain (BioLabs) in 10 mM Tris ...
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bioRxiv - Biochemistry 2024Quote: ... the larger DNA fragment of pNLX.Luc.R-.ΔAvrII73 following digestion with PflMI and AgeI restriction enzymes (New England Biolabs) was assembled with 1.85-kb synthetic fragments (Twist Bioscience ...
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bioRxiv - Biochemistry 2024Quote: ... BsmBI-v2 0.5 µL (R0739L, New England Biolabs, Ipswich, Massachusetts, United States), pYTK001 0.5 µL ...
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bioRxiv - Biochemistry 2024Quote: ... The on- and off-target genomic sites (experimentally determined from a previous study) were PCR amplified with Phusion plus DNA polymerases (New England Biolabs) and locus-specific primers having tails complementary to the Truseq adapters ...
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bioRxiv - Biochemistry 2024Quote: ... T4 ligase 0.5 µL (M0202L, New England Biolabs, Ipswich, Massachusetts, United States), BsaI-HF®v2 0.5 µL (R3733L ...
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bioRxiv - Biochemistry 2024Quote: ... BsaI-HF®v2 0.5 µL (R3733L, New England Biolabs, Ipswich, Massachusetts, United States), DNA parts 0.5 µL (20 fm) ...
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bioRxiv - Biochemistry 2024Quote: ... cells were harvested 3 days after electroporation and genomic DNA was extracted with 100 μL lysis buffer (10 mM Tris-HCl, pH 7.5, 0.05% SDS, 25 μg/mL proteinase K (NEB)) at 37 °C with incubation for 1 hour ...
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bioRxiv - Biochemistry 2024Quote: ... T4 ligase 0.5 µL (M0202L, New England Biolabs, Ipswich, Massachusetts, United States), BsmBI-v2 0.5 µL (R0739L ...
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bioRxiv - Biochemistry 2024Quote: ... Template DNA was removed by treatment with DpnI (New England Biolabs), and the PCR products were subsequently 5’ phosphorylated using T4 polynucleotide kinase (New England Biolabs ...
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bioRxiv - Biochemistry 2024Quote: ... PCR reactions were carried out using Taq polymerase (New England Biolabs) and contained 2.5 µM gene-specific primers that flanked the intron ...
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bioRxiv - Biochemistry 2024Quote: ... The PCR product was purified by phenol chloroform extraction and RNA was synthesized using the HiScribe T7 Quick High Yield RNA Synthesis Kit (NEB). 1 ug of starting linear DNA template was used and transcription was performed for 2hrs at 37°C ...
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bioRxiv - Biochemistry 2024Quote: ... and the PCR products were subsequently 5’ phosphorylated using T4 polynucleotide kinase (New England Biolabs) and self-ligated by T4 DNA ligase (New England Biolabs ...