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Citations for New England Biolabs :
651 - 700 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... BsmBI-v2 0.5 µL (R0739L, New England Biolabs, Ipswich, Massachusetts, United States), pYTK001 0.5 µL ...
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bioRxiv - Biochemistry 2024Quote: ... The on- and off-target genomic sites (experimentally determined from a previous study) were PCR amplified with Phusion plus DNA polymerases (New England Biolabs) and locus-specific primers having tails complementary to the Truseq adapters ...
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bioRxiv - Biochemistry 2024Quote: ... T4 ligase 0.5 µL (M0202L, New England Biolabs, Ipswich, Massachusetts, United States), BsaI-HF®v2 0.5 µL (R3733L ...
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bioRxiv - Biochemistry 2024Quote: ... BsaI-HF®v2 0.5 µL (R3733L, New England Biolabs, Ipswich, Massachusetts, United States), DNA parts 0.5 µL (20 fm) ...
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bioRxiv - Biochemistry 2024Quote: ... cells were harvested 3 days after electroporation and genomic DNA was extracted with 100 μL lysis buffer (10 mM Tris-HCl, pH 7.5, 0.05% SDS, 25 μg/mL proteinase K (NEB)) at 37 °C with incubation for 1 hour ...
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bioRxiv - Biochemistry 2024Quote: ... T4 ligase 0.5 µL (M0202L, New England Biolabs, Ipswich, Massachusetts, United States), BsmBI-v2 0.5 µL (R0739L ...
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bioRxiv - Biochemistry 2024Quote: ... Template DNA was removed by treatment with DpnI (New England Biolabs), and the PCR products were subsequently 5’ phosphorylated using T4 polynucleotide kinase (New England Biolabs ...
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bioRxiv - Biochemistry 2024Quote: ... PCR reactions were carried out using Taq polymerase (New England Biolabs) and contained 2.5 µM gene-specific primers that flanked the intron ...
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bioRxiv - Biochemistry 2024Quote: ... The PCR product was purified by phenol chloroform extraction and RNA was synthesized using the HiScribe T7 Quick High Yield RNA Synthesis Kit (NEB). 1 ug of starting linear DNA template was used and transcription was performed for 2hrs at 37°C ...
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bioRxiv - Biochemistry 2024Quote: ... and the PCR products were subsequently 5’ phosphorylated using T4 polynucleotide kinase (New England Biolabs) and self-ligated by T4 DNA ligase (New England Biolabs ...
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bioRxiv - Biochemistry 2024Quote: ... and 20U RNase inhibitor (NEB). The translation reactions were incubated in 384 well plates at 30°C for 1hr ...
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bioRxiv - Biochemistry 2024Quote: ... Parental plasmids were digested by adding 5 µL 10x cutsmart buffer (NEB), and 1 µL DpnI (NEB ...
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bioRxiv - Biochemistry 2024Quote: ... A colony PCR using Taq Polymerase (New England Biolabs) on positive resistant colonies can help identifying false positives for Ampicillin resistance ...
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bioRxiv - Biochemistry 2024Quote: ... coli (Suppl. Table 1) were cloned into a pBAD24 with ampicillin resistance using the USER cloning method (New England Biolabs, Table 2). All sequences were cloned including a 10x histidine tag located in the N-terminus for BtuG1-3 and the C-terminus for BtuG2 and all its mutants ...
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bioRxiv - Biochemistry 2024Quote: ... reactions were quenched on ice with STOP Buffer (0.4 U proteinase K (NEB), 18 mM EDTA ...
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bioRxiv - Biophysics 2024Quote: ... coli NiCo21(DE3) strain (NEB). Cells were grown at 37°C supplemented with 2XYT medium (Melford ...
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bioRxiv - Biophysics 2024Quote: Cloning procedures involving PCR were performed using Q5 High-fidelity polymerase (New England Biolabs) according to manufacturer’s protocol ...
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bioRxiv - Biophysics 2024Quote: ... The isolated RNA underwent enzymatic capping (NEB) and polyadenylation (Thermo Fisher) ...
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bioRxiv - Biophysics 2024Quote: ... coli strain (NEB, Ipswich, MA, USA) were used for the gene cloning ...
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bioRxiv - Biophysics 2024Quote: ... Gibson Assembly Cloning Kit (NEB, Ipswich, MA, USA) and 5-alpha E ...
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bioRxiv - Biochemistry 2024Quote: ... Total RNA was extracted using the Monarch Total RNA Miniprep Kit (T2010S, New England Biolabs) and cDNA was synthesized using the SuperScript IV First-Strand Synthesis System (18091050 ...
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bioRxiv - Biochemistry 2024Quote: Ribosome-protected fragments were dephosphorylated using T4 Polynucleotide Kinase (M0201, New England Biolabs). Dephosphorylated RNA was extracted using ROTI Acqua P/C/I for RNA extraction (X985.1 ...
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bioRxiv - Biochemistry 2024Quote: ... the collected cell pellets were processed using Monarch RNA extraction kit (New England BioLabs). The RNA samples were then quantified with nanodrop and normalized before cDNA synthesis (Lambda Biotechnologies) ...
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bioRxiv - Biochemistry 2024Quote: ... Libraries were PCR amplified using Phusion DNA Polymerase (M0530, New England Biolabs). Quality assessment of the libraries and sequencing were performed by the NGS platform of the University of Bern.
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bioRxiv - Biochemistry 2024Quote: ... RNA was ligated using T4 RNA ligase 2 (M0242, New England Biolabs) to a preadenylated linker and purified with ROTI Aqua P/C/I for RNA extraction and precipitated with NaOAc ...
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bioRxiv - Biochemistry 2024Quote: ... The plasmids were transformed into either BL21(DE3) (NEB) or OrigamiB(DE3 ...
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bioRxiv - Biochemistry 2024Quote: ... Fragments were assembled into the pBAD24 vector using HiFi DNA Assembly Kit (New England Biolabs, Ipswich, MA) with 1-hour ...
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bioRxiv - Biochemistry 2024Quote: ... followed by affinity-purification with amylose resin (New England Biolabs). The amylose eluates were purified further by anion exchange chromatography using Source 15Q Resin (Cytiva) ...
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bioRxiv - Biochemistry 2024Quote: ... The HDV sequence was cloned into the XbaI/EcoRV sites of the AVA421 vector to enable linearization for run-off transcription by digestion with EcoRV-HF (NEB) in a reaction containing 1X CutSmart buffer and 0.4 U/μl EcoRV-HF.
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bioRxiv - Biochemistry 2024Quote: ... a pET-derived vector that is normally used for T7 RNA polymerase-dependent protein expression (AVA421) was linearized by digestion with XbaI (NEB) in a reaction containing 1X CutSmart buffer and 0.5 U/μl XbaI to be used for run-off transcription using T7 RNA polymerase to transcribe the first 26 nucleotides following the T7 promoter sequence to generate the standard model RNA substrate ...
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bioRxiv - Biochemistry 2024Quote: ... and 1 µL DpnI (NEB) directly to the PCR reaction mixture after PCR and continued incubation for 1h at 37°C ...
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bioRxiv - Biochemistry 2024Quote: ... coli NEB C2566 cells (New England Biolabs). The cells were grown at 37°C in TB medium for HINT1 ...
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bioRxiv - Bioengineering 2024Quote: ... and mCherry inserts were generated via PCR and cloned into the linearized viral backbone as a single fusion protein using HiFi cloning mix (NEB). H2B-iRFP nuclear marker was (Addgene Plasmid #90237) ...
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bioRxiv - Biophysics 2024Quote: ... coli SHuffle T7 Express (New England Biolabs, Ipswich, MA) cells were transformed with 100 ng pET32-Trx-LqhαIT and ...
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bioRxiv - Biophysics 2024Quote: ... Monarch RNase A (NEB) (0.1 ng/mL in final concentration ...
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bioRxiv - Biochemistry 2024Quote: ... ExoIII (NEB#M0206V), APE1 (NEB#M0282S) ...
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bioRxiv - Biochemistry 2024Quote: ... 1 µL T4 DNA Ligase (NEB), 100 ng DNA (oligo 3) ...
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bioRxiv - Biochemistry 2024Quote: ... and LbCas12a (NEB#M0653S) were bought from New England Biolabs (Ipswich ...
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bioRxiv - Biochemistry 2024Quote: ... APE1 (NEB#M0282S), T7 exonuclease (T7 exo ...
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bioRxiv - Biochemistry 2024Quote: ... T7 exonuclease (T7 exo) (NEB#M0263L) and LbCas12a (NEB#M0653S ...
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bioRxiv - Biochemistry 2024Quote: ... at 37°C for 30 min and purified by Monarch RNA Cleanup Kit (New England Biolabs, Massachusetts, USA). The concentration of crRNA was determined by Nanodrop 2000 (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2024Quote: ... R4: 5’-TAGGTACAGTCCTCAGGTGTCTT[AmC6dT]T) were coupled with SNAP-substrate NHS ester (BG-GLA-NHS, NEB). The DNA strands at 1 mM concentration were incubated with 10 mM of BG-GLA-NHS and reactions were cleaned up using a Bio-Spin P-30 column (Bio-rad).
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bioRxiv - Biophysics 2024Quote: ... Splint ligation reaction was done by mixing DNA in equal molar ratios of 1 mM in a 20 μl reaction and annealed in 1✕ ligation buffer (NEB) over 4 hours using a thermocycler (bio-rad) ...
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bioRxiv - Biophysics 2024Quote: ... N-glycosylation was removed by a 1-hour incubation with PNGase F (500 U, NEB) and following digest with sequence-grade trypsin (Promega ...
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bioRxiv - Bioengineering 2024Quote: ... grown in 5-alpha competent Escherichia coli (NEB, Ipswich, MA) and purified using the PureYield MidiPrep System (Promega ...
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bioRxiv - Biochemistry 2024Quote: ... isolated chaperones and empty 70S ribosomes (NEB) were prepared as controls ...
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bioRxiv - Biochemistry 2024Quote: ... coli cells (NEB) were transformed with plasmids encoding β-gal RNC constructs and grown in ZYM-5052 autoinduction media 106 for 18 h at 37 °C ...
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bioRxiv - Biochemistry 2024Quote: ... deletions and point mutations were introduced using site-directed mutagenesis with Q5 or Phusion polymerases (NEB). All constructs used in this study (Table S10 ...
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bioRxiv - Bioengineering 2024Quote: ... Genotyping PCR was then conducted using Q5 polymerase (NEB) for genomic DNA and cDNA ...
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bioRxiv - Bioengineering 2024Quote: The 5’ RACE protocol using the template-switching reverse transcriptase enzyme mix from NEB was executed following the manufacturer’s guidelines ...