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Citations for New England Biolabs :
651 - 700 of 10000+ citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... Coli (NEB, C2527I) were grown on LB (Luria-Bertani ...
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bioRxiv - Biochemistry 2024Quote: N-term-His-ELMO1C438A construct was generated from N-term-His-ELMO1 construct obtained above using Q5® Site-Directed Mutagenesis Kit (New England BioLabs, cat # E0554S), with the following primers.
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bioRxiv - Biochemistry 2024Quote: ... using Q5® Site-Directed Mutagenesis Kit (New England BioLabs, cat # E0554S) with the following primers.
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bioRxiv - Biochemistry 2024Quote: ... lentiviral plasmids were constructed using a pLX317 backbone in competent Escherichia coli (New England Biolabs, C3050H). Lenti-XTM 293T (Takara ...
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bioRxiv - Biochemistry 2024Quote: All gene insertions were carried out using the NEBuilder HiFi DNA Assembly kit (New England Biolabs).
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bioRxiv - Biochemistry 2024Quote: ... which was dephosphorylated at the 5’-end with 3 µl of Quick-CIP (5000 U/µl, NEB) in a total volume of 20 µl according to manufactureŕs instructions ...
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bioRxiv - Biochemistry 2024Quote: ... coli BL21(DE3) (NEB) using the recommended heat-shock protocol and plated onto LB Miller agar supplemented with 50 mg mL-1 kanamycin before being incubated overnight at 37 °C.
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bioRxiv - Biochemistry 2024Quote: ... B2R phosphorylation site mutants mentioned in the manuscript were generated by site-directed mutagenesis using Q5 Site-directed mutagenesis kit (NEB, Cat. No. E0554). All DNA constructs used in this study were verified by sequencing from Macrogen ...
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bioRxiv - Biochemistry 2024Quote: ... NEBuilder HiFi DNA Assembly Master Mix(NEB) was then used to insert them into 6x histidine tagged PanZ(HTSD1.10 ...
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bioRxiv - Bioengineering 2024Quote: ... and 10 U of DNA polymerase I (NEB) was added to the samples on ice for a final volume of 50 μl ...
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bioRxiv - Biochemistry 2024Quote: Gibson assembly (New England Biolabs) was used to generate all constructs ...
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bioRxiv - Biochemistry 2024Quote: ... 2 μL of T4 DNA ligase (NEB M0202S) was added to the mixture and incubated overnight at room temperature to seal nicks on DNA ...
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bioRxiv - Biochemistry 2024Quote: ... 100 μL of 1 unit mL-1 SfoI restriction enzyme (NEB) was injected to generate blunt-end DNA molecules.
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bioRxiv - Biochemistry 2024Quote: ... 200 U of T4 ligase in T4 ligase buffer (NEB), 0.1 mg/mL BSA (NEB ...
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bioRxiv - Biochemistry 2024Quote: ... 0.5 µL T4 DNA Ligase (NEB #M0202L), 0.5 µL T4 Polynucleotide Kinase (NEB #M0201L) ...
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bioRxiv - Biochemistry 2024Quote: ... 0.1 mg/mL BSA (NEB) and 40 GoldenGate digestion ligation cycles (80) ...
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bioRxiv - Biochemistry 2024Quote: ... 0.5 µL T4 Polynucleotide Kinase (NEB #M0201L), and 0.5 µL DpnI (NEB #R0176L ...
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bioRxiv - Biochemistry 2024Quote: ... coli (NEB #C2984H ...
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bioRxiv - Biochemistry 2024Quote: ... 200 U of T4 ligase in T4 ligase buffer (NEB), 0.1 mg/mL BSA (NEB ...
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bioRxiv - Biochemistry 2024Quote: ... and 0.5 µL DpnI (NEB #R0176L) for 2 hours at 25 °C ...
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bioRxiv - Biochemistry 2024Quote: ... The PCR reaction was DpnI (New England Biolabs) digested at 37 °C for 2 h without prior clean-ups ...
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Autorepression of Yeast Hsp70 co-chaperones by intramolecular interactions involving their J-domainsbioRxiv - Biochemistry 2024Quote: ... SIS1VR 5’- CCAATCTGTTCGCGGTGAGCCTCA-3’) by Gibson Assembly (NEB). All constructs were confirmed by sequencing.
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bioRxiv - Cancer Biology 2024Quote: ... the pooled oligo library was amplified using NEB Next High-Fidelity PCR Master Mix (New England Biolabs, cat#M0541S). The PCR product was digested with Esp3I (BsmBI ...
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bioRxiv - Cell Biology 2024Quote: ... or (Q71L for Arf1) and dominant negative (T27N for Arf6) or (T31N for Arf1) by using site-directed mutagenesis (SDM kit from NEB) kit ...
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bioRxiv - Cell Biology 2024Quote: ... or N-terminus of PEmax (PEmax-hP53DD-P2A-hMLH1dn) under the constitutive expression from a CMV promoter using Hifi DNA assembly (NEB). To generate the other two all-in-one prime editor with linkages in between the three components ...
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bioRxiv - Cell Biology 2024Quote: ... while lenti-pegRNA-GG-acceptor or lenti-tmpknot-GG-acceptor was digested with BsmBI-v2 (NEB). The pegRNA or epegRNA plasmid was cloned using Golden-Gate assembly ...
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bioRxiv - Cell Biology 2024Quote: ... pU6- or pU6-tmpknot-GG-acceptor was digested with BsaI-HFv2 (NEB), while lenti-pegRNA-GG-acceptor or lenti-tmpknot-GG-acceptor was digested with BsmBI-v2 (NEB) ...
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bioRxiv - Cell Biology 2024Quote: ... gibco) supplemented with 10% fetal calf serum (eurobio scientific) and 1% ZellShield cell culture contamination preventive (Minerva-Biolabs). Cells were passaged at 80-90% confluency ...
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bioRxiv - Cell Biology 2024Quote: ... The editing region was amplified with PCR using Q5 High-Fidelity 2X Master Mix (NEB) followed by purification the PCR product using the QIAquick PCR Purification Kit (QIAGEN) ...
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bioRxiv - Cell Biology 2024Quote: ... orthovanadate (200 nM; New England Biolabs, P0758S) and β-glycerophosphate (25 mM ...
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bioRxiv - Cell Biology 2024Quote: ... Genomic DNA was isolated from ∼2×106 cells using the Monarch DNA purification Kit (New England Biolabs). For genotyping ...
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bioRxiv - Cancer Biology 2024Quote: ... before ligation to microsatellite expansion adapters containing an EcoRV restriction site (listed in Supplemental Table 1) using the T4 DNA Ligase Kit (NEB) at a 5:1 ratio overnight at 16°C ...
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bioRxiv - Cancer Biology 2024Quote: ... Adapter sequences were then removed by restriction digest (PCR reaction product, 1X rCutSmart NEB Buffer, 5 U EcoRV-HF (NEB)) at 37°C for 1 hour ...
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bioRxiv - Cancer Biology 2024Quote: ... Adapter sequences were then removed by restriction digest (PCR reaction product, 1X rCutSmart NEB Buffer, 5 U EcoRV-HF (NEB)) at 37°C for 1 hour ...
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bioRxiv - Cell Biology 2024Quote: ... washed and incubated with T cell medium containing 5 μM (Snap-Cell Block S9106S, New England Biolabs, NEB) for 30 min at 37°C ...
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bioRxiv - Cell Biology 2024Quote: ... washed and incubated with T cell medium containing 5 μM (Snap-Cell Block S9106S, New England Biolabs, NEB) for 30 min at 37°C ...
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bioRxiv - Cell Biology 2024Quote: ... or 3 * 106 cells with the lowest or highest 5% of Spry4:H2B-Venus fluorescence were FAC sorted and their DNA isolated by column-based genomic DNA purification (Monarch Genomic DNA Purification Kit, NEB). For reference ...
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bioRxiv - Cell Biology 2024Quote: ... using BbsI (NEB) overhangs following Ran et al. ...
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bioRxiv - Cell Biology 2024Quote: ... the plasmids were linearised by the DraI restriction enzyme (New England Biolabs; R0129), a dual cutter for the plasmid ...
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bioRxiv - Cell Biology 2024Quote: ... was linearised with the BsaI restriction enzyme (New England Biolabs; #R0535). The linearised plasmid was run on agarose gel and purified with QIAquick Gel Extraction Kit (Qiagen ...
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bioRxiv - Biophysics 2024Quote: ... Plasmids created in this study were assembled using the Gibson Assembly Cloning Kit (New England Biolabs). All plasmid sequences were confirmed by whole plasmid sequences services provided my Plasmidsaurus SNPsaurus LLC.
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bioRxiv - Cancer Biology 2024Quote: cDNA was generated from RNA using components from a NEBNext® Single Cell/Low Input cDNA Synthesis & Amplification Module (New England Biolabs, cat. no. E6421S), MAS-Seq for 10x Single Cell 3’ kit (PacBio ...
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bioRxiv - Cancer Biology 2024Quote: ... cDNA was generated from RNA using components from a NEBNext® Single Cell/Low Input cDNA Synthesis & Amplification Module (New England Biolabs, cat. no. E6421S). The RNA Samples were diluted ...
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bioRxiv - Biophysics 2024Quote: ... and R88 using the Q5 site-directed mutagenesis kit (NEB). Giant spheroplasts were generated as described previously (Martinac et al. ...
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bioRxiv - Biophysics 2024Quote: ... The plasmid was linearized with NheI-HF (New England Biolabs) and purified using QIAquick PCR Purification Kit (QIAGEN) ...
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bioRxiv - Biophysics 2024Quote: ... 25 mU/mL Apyrase (New England Biolabs), and 100 μM TCEP at room temperature for 2 h ...
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bioRxiv - Biophysics 2024Quote: ... Plasmids were isolated from NEB Stable Competent cells using the QIAGEN Plasmid Plus Midi Kit.
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bioRxiv - Biophysics 2024Quote: ... was obtained from Addgene (#31207) and subsequently inserted into a pGEX-6p-1 vector (Cytiva, 28954648) using Gibson Assembly (New England Biolabs, E2611L). The resulting construct was expressed in Escherichia coli BL21(DE3 ...
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bioRxiv - Biophysics 2024Quote: ... coli chemically-competent cells (New England BioLabs). Single colonies of transformed cells from agar plates containing 100 μg/mL ampicillin and grown at 37 °C were used for starter cultures using 50 mL LB media in 250 mL shake flasks containing 100 μg/mL ampicillin ...
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bioRxiv - Cell Biology 2024Quote: ... the DNA is linearized by KpnI-HF from Biolabs. The linearized DNA was concentrated to 1 μg/μL by ethanol precipitation.