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Citations for New England Biolabs :
4151 - 4200 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The 1392-bp region comprising the E9 region was PCR amplified from genomic DNA and cloned upstream of the DPYD promoter using KpnI and SacI sites (New England Biolabs). A vector containing the A allele of rs4294451 within E9 was confirmed by Sanger sequencing ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... and ligated into digested BsmBI-digested (New England Biolabs) lentiGuide-Puro vector (a gift from Feng Zhang ...
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bioRxiv - Neuroscience 2024Quote: ... were amplified by PCR employing Q5 polymerase (NEB#M0494). Subsequently ...
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bioRxiv - Molecular Biology 2024Quote: ... PCR fragments were cloned into psiCHECK-2 using the restriction enzymes XhoI/NotI or SglI/NOTI (New England Biolabs). Ligations were performed with a quick ligation kig (New England Biolabs ...
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bioRxiv - Molecular Biology 2024Quote: ... Phusion High-Fidelity PCR Master Mix with HF Buffer (NEB) following the manufacturer’s instruction and specific primers (see primer list) ...
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bioRxiv - Molecular Biology 2024Quote: ... Ligations were performed with a quick ligation kig (New England Biolabs) and ligation products were transformed into DH5α competent E ...
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bioRxiv - Microbiology 2024Quote: ... the RNA pellet was resuspended in 3’ ligation reaction buffer 1x and subjected to library preparation using the NEBNext® Small RNA Library Prep Set for Illumina® (NEB, #E7330S). Specific primers for V ...
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bioRxiv - Genomics 2024Quote: ... sections were cleared for 24-48 hours with a mixture of VIZGEN Clearing Solution (VIZGEN 20300003) and Proteinase K (New England Biolabs P8107S) according to the manufacturer’s instructions ...
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bioRxiv - Genetics 2024Quote: ... T4 DNA Ligase (NEB) was used for ligation reactions ...
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bioRxiv - Microbiology 2024Quote: ... Up to 1ug of RNA is then converted into cDNA using the NEB LunaScript® RT Supermix Kit (NEB #E3010) followed by qPCR using the NEB Luna Universal qPCR Master Mix (NEB #M3003) ...
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bioRxiv - Microbiology 2024Quote: ... followed by qPCR using the NEB Luna Universal qPCR Master Mix (NEB #M3003). The ΔΔCT method was used for quantification of relative transcript level normalized to YPD samples (44).
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bioRxiv - Microbiology 2024Quote: ... DNA was amplified from cDNA using 10µl of 5x Q5 reaction buffer and 2.5µl Q5 enzyme (NEB), 0.5µl 100mM dNTPs ...
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bioRxiv - Genomics 2024Quote: ... The bPCA library was assembled overnight by temperature-cycle ligation using T4 ligase (New England Biolabs) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2024Quote: ... 3.5 U of Large (Klenow) fragment (NEB) and 6 U of T4 DNA polymerase for 30 minutes at 20°C in a total volume of 33 μL ...
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bioRxiv - Genomics 2024Quote: ... End repair was performed in T4 ligase reaction buffer (New England Biolabs, NEB), 0.4mM of dNTPs ...
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bioRxiv - Genomics 2024Quote: RRS-lysO and RRS-dusC sequences were amplified from MG1655 genomic DNA using Q5 High-Fidelity master mix (New England Biolabs) and pairs of primers containing a MfeI restriction site at each end ...
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bioRxiv - Genomics 2024Quote: M13seq primer (5’-GACGTTGTAAAACGACGGC-3’) was labeled with 32P at 5’-end by T4 polynucleotide kinase (New England Biolabs). Primer/template (p/t ...
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bioRxiv - Genomics 2024Quote: ... Psoralen pull-down DNA was further purified using a Monarch PCR & DNA clean-up kit (New England Biolabs), eluted in 15 μl of EB buffer (10 mM Tris ...
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bioRxiv - Molecular Biology 2024Quote: ... The supernatant was incubated with amylose resin (New England Biolabs, E8021S) for 1 h before elution with lysis buffer + 10 mM maltose ...
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bioRxiv - Molecular Biology 2024Quote: ... Proteinase K and RNAse A were added at the concentration recommended by NEB. Proteins were next separated from the gDNA by adding 40 µL of protein separation solution ...
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bioRxiv - Molecular Biology 2024Quote: ... Genomic DNAs (gDNAs) were extracted using the Monarch HMW DNA extraction kit for Tissue from New England Biolabs (NEB) with the following modifications ...
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bioRxiv - Molecular Biology 2024Quote: ... Library was prepared using Ultra II Directional RNA Library Prep Kit (NEB, Cat #E7765) for all samples ...
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bioRxiv - Molecular Biology 2024Quote: ... RNA was DNase treated then 1µg was reverse transcribed using Protoscript II reverse transcriptase (New England Biolabs). qPCR was performed using LUNA SYBR green reagent (New England Biolabs ...
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bioRxiv - Molecular Biology 2024Quote: ... Libraries were prepared using the NEBNext Ultra™ II Directional RNA Library Prep Kit for Illumina (New England Biolabs). sPOINT was performed in the same manner with the following difference ...
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bioRxiv - Microbiology 2024Quote: ... PCR amplifications were carried out using Q5® High-Fidelity DNA Polymerase (New England Biolabs) for cloning and DreamTaq™ DNA Polymerase (Thermo Fisher ...
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bioRxiv - Microbiology 2024Quote: ... Restriction and modification enzymes were used following to the manufacturer’s instructions (New England Biolabs). PCR amplifications were carried out using Q5® High-Fidelity DNA Polymerase (New England Biolabs ...
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bioRxiv - Cell Biology 2024Quote: ... Alice Ting’s plasmid (30) via PCR using Q5 high-fidelity (M0492, NEB). Next ...
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bioRxiv - Cell Biology 2024Quote: ... Loss-of-function mutations were generated using site directed mutagenesis using Q5 Site-directed mutagenesis kit (E0552S, NEB) with the HA::SQSTM1/p62 plasmid as template (Addgene#280227) ...
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bioRxiv - Cancer Biology 2024Quote: ... 20 sec 72°C) with Phusion polymerase (New England Biolabs (NEB)) ...
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bioRxiv - Cancer Biology 2024Quote: ... RNA of 2×106 cells was extracted with the Monarch Total RNA Miniprep Kit with “on column” DNase I treatment (NEB). cDNA was generated by using LunaScript RT SuperMix Kit (NEB ...
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bioRxiv - Cancer Biology 2024Quote: ... cDNA was generated by using LunaScript RT SuperMix Kit (NEB) and qPCR was performed with the Luna universal qPCR Master Mix (NEB ...
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bioRxiv - Cancer Biology 2024Quote: ... and qPCR was performed with the Luna universal qPCR Master Mix (NEB) (primer sequences in Table S1).
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bioRxiv - Evolutionary Biology 2024Quote: ... Library enrichment was performed using either a Q5U® Hot Start High-Fidelity DNA Polymerase (New England Biolabs) or the NEBNext Ultra II Q5DNA polymerase (Table S1) ...
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bioRxiv - Biophysics 2024Quote: ... mRNA was isolated using Poly(A) mRNA magnetic isolation module (New England BioLabs #E7490). Preparation of libraries followed the manufacturer’s protocol (Version 2.2 05/19) ...
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bioRxiv - Biophysics 2024Quote: ... Methylated adaptors (NEBNext Multiplex Oligos for Illumina; NEB #E7535L) were ligated to the product from the preceding step using NEBNext Ultra Ligation Module (NEB #7445L) ...
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bioRxiv - Biophysics 2024Quote: ... WGBS libraries were prepared using the NEBNext Ultra DNA Library Prep Kit (NEB #E7370L) according to manufacturer’s instructions with modifications ...
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bioRxiv - Cancer Biology 2024Quote: ... Libraries were prepared using Ultra II FS library preparation kit (New England Biolabs) and amplified with KAPA HiFi HotStart Real-time PCR using KAPA P5 and P7 primers (KAPA Biosystems).
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bioRxiv - Cancer Biology 2024Quote: ... ChIP-seq libraries were prepared using the NEBNext Ultra II DNA Library Prep Kit (NEB, E7645L) and single-end (1 × 75 bp ...
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bioRxiv - Developmental Biology 2024Quote: ... Total RNA was extracted from each pool using the Monarch® Total RNA Miniprep Kit (New England Biolabs) including on-column DNase treatment ...
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bioRxiv - Developmental Biology 2024Quote: ... mRNA was enriched using the New England Biolab’s (NEB) NEBNext Poly(A ...
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bioRxiv - Developmental Biology 2024Quote: ... mRNA isolation module for samples with RINs greater than 7 and libraries were prepared using the NEBNext Ultra II directional RNA library preparation kit (NEB, E7760). QC was then performed on these libraries using an Agilent Bioanalyzer 2100 and the libraries were quantified using fluorometric methods ...
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bioRxiv - Cell Biology 2024Quote: ... the Q5 Site-Directed Mutagenesis Kit (NEB) was used to delete the miniTurbo sequence from the above pLVX-KRT5-miniTurbo-FLAG-IRES-Puro and pLVX-KRT6A-miniTurbo-FLAG-IRES-Puro constructs ...
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bioRxiv - Cell Biology 2024Quote: ... with C-terminal fluorescent tags using seamless cloning (HiFi DNA Assembly Master Mix, New England Biolabs). The pLVX-KRT5-mNG-IRES-Puro construct includes an mNeonGreen (Allele Biotechnology ...
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bioRxiv - Genetics 2024Quote: ... 10ul NEBNExt Quick T4 DNA Ligase (NEB E6056S), and 5ul Ligation Adapter ...
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bioRxiv - Genetics 2024Quote: ... Golden Gate Assembly reaction was carried out in a 100ul reaction containing 2.5U Esp3I (Thermo ER0452) and 1000U T4 DNA Ligase in T4 DNA Ligase reaction buffer (NEB M0202L). The reaction mix was incubated for 31 cycles alternating between 37deg ...
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bioRxiv - Cell Biology 2024Quote: ... Reactions were quenched by addition of EDTA to 50 mM and proteins were removed by treatment with proteinase K (20 units/ml) (NEB) and SDS (0.25% ...
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bioRxiv - Cell Biology 2024Quote: ... and was resuspended in 65 μl 1X rCutSmart buffer (NEB). Samples were divided into three 20 μl aliquots that were treated (or mock treated ...
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bioRxiv - Cell Biology 2024Quote: ... 8 U/μl Salt-T4 DNA ligase (NEB #M0467)) ...
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bioRxiv - Cell Biology 2024Quote: ... Primer extension was performed with 0.006 U/μl Q5 (NEB, #M0491) and 200 μM each of dATP/dCTP/dTTP at 21°C for 10 min and an additional 15 min incubation with 25 nM recombinant human RPA (a gift from Sarah W ...
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bioRxiv - Cell Biology 2024Quote: ... coli ExoI (#M0293, NEB) in 67 mM Glycine-NaOH (pH 9.5) ...