Labshake search
Citations for New England Biolabs :
4051 - 4100 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Molecular Biology 2024Quote: ... mRNA sequencing libraries were generated using the poly-A selection module in the NEBNext UltraII Directional RNA Library Prep Kit (NEB E7760) and sequenced on the Illumina HiSeq 2500 sequencer with single-end 50 cycles ...
-
bioRxiv - Molecular Biology 2024Quote: ... Protein-coding or lncRNA genes with adjusted P-value < 0.05 and absolute log2 fold change > 1 (NEB Directional RNA-seq) or with adjusted P-value < 0.05 and absolute log2 fold change > 0 (QuantSeq 3’ mRNA-seq ...
-
bioRxiv - Molecular Biology 2024Quote: ... Quantitative RT-PCR was conducted with the Luna Universal One-Step RT-qPCR Kit (NEB, E3005) on a Bio-Rad CFX96 Real-Time PCR Detection System (Bio-Rad ...
-
bioRxiv - Molecular Biology 2024Quote: ... and the luciferase gene was replaced with the cGAS or EGFP sequences using NEBuilder HiFi DNA Assembly Master Mix (NEB E2621). The pAAV:cTnT::GFP-icGAS vector was generated by inserting the catalytically-inactive cGAS (icGAS ...
-
bioRxiv - Molecular Biology 2024Quote: ... The R1R DNA adapter was adenylated by using a 5’ DNA Adenylation kit (New England Biolabs) and then ligated to the 3’ end of the cDNA by using Thermostable 5’ App DNA/RNA Ligase (New England Biolabs ...
-
bioRxiv - Molecular Biology 2024Quote: ... Libraries were qualified on Agilent 2100 Bioanalyzer using High Sensitivity DNA Kit and quantified by qPCR using NEBNext Library Quant Kit for Illumina (NEB, E7630). Equal molarity of each library was pooled and subjected to sequencing on Illumina NovaSeq 6000 platform at the McGill University and Génome Québec Innovation Centre to generate 100 bp paired-end reads.
-
bioRxiv - Molecular Biology 2024Quote: ... PCR amplification was performed using NEB primers for 15-16 cycles using the Q5 Hot Start HiFi PCR Master Mix (NEB). The PCR-amplified library was purified using Ampure XP beads and its quality was assessed on a Bioanalyzer 2100 system (Agilent) ...
-
bioRxiv - Cell Biology 2024Quote: ... freshly supplemented with 10 μl proteinase K (P8107S, New England Biolabs). Plates were stored slightly tilted to ensure the coverslip was completely covered with digestion solution and incubated at room temperature overnight ...
-
bioRxiv - Cell Biology 2024Quote: ... Total lysates (200 µg each) were incubated with or without 400 U lambda protein phosphatase (New England Biolabs, #P0753S) and 1 mM MnCl2 at 30 °C for 30 min ...
-
bioRxiv - Molecular Biology 2024Quote: ... 2 units of T4 polynucleotide kinase (NEB, M0201S), 0.09 nM dNTPs and 0.045 μg/μL of BSA at 12°C for 30 min ...
-
bioRxiv - Molecular Biology 2024Quote: ... Samples were then treated with 5 units of Antartic phosphatase (NEB M0289S) in a final volume of 50 µL for one hour at 37°C ...
-
bioRxiv - Cell Biology 2024Quote: ... MBP::CDC-42 coding sequence was inserted into the pMAL-c5X plasmid (NEB). The S71A mutation was introduced into the pMALc-MBP::CDC-42 plasmid with the QuickChange II XL Site directed mutagenesis kit (#200521 ...
-
bioRxiv - Cell Biology 2024Quote: ... 1 mM EDTA) and passed through an amylose resin column (NEB#E8021L). Columns were washed prior to recombinant protein elution with 10 mM maltose in column buffer.
-
bioRxiv - Developmental Biology 2024Quote: ... cDNA was synthesized using M-MuLV Reverse Transcriptase (New England Biolabs) with random hexamer primers (Integrated DNA Technologies) ...
-
bioRxiv - Cell Biology 2024Quote: ... site directed mutagenesis was performed using a Q5 polymerase (New England BioLabs). All oligonucleotide sequences used for site-directed mutagenesis can be found in Table EV2.
-
bioRxiv - Molecular Biology 2024Quote: ... Ribosome foot-printing was performed on samples collected as mentioned above by adding 1.25 U/μl RNase I (NEB, #M0243L) to 500 μl clarified lysate and incubating samples on a rotator mixer for 45 min at room temperature ...
-
bioRxiv - Molecular Biology 2024Quote: ... All treated RNA samples were used for library preparation using NEBNext Multiplex Small RNA Library Prep Set for Illumina (NEB #E7300/7580) according to the manufacturer protocol ...
-
bioRxiv - Molecular Biology 2024Quote: ... we performed a streptavidin extraction (New England Biolabs, 150 μl per sample) for 30 minutes at room temperature using streptavidin-linked magnetic beads ...
-
bioRxiv - Molecular Biology 2024Quote: ... to a final volume of 800 µl after washing it with 1x T4 ligase buffer (NEB) (2,500 xg ...
-
bioRxiv - Molecular Biology 2024Quote: ... and incubated in buffer IIa (0.2% SDS, 1x NEBuffer 3.1; NEB) for 10 min at 50 °C ...
-
bioRxiv - Molecular Biology 2024Quote: ... Library preparation was performed on beads using NEBNext ChIP-Sequencing Library Prep Master Mix Set for Illumina (NEB). The manufacturer’s instructions were followed ...
-
bioRxiv - Molecular Biology 2024Quote: ... and 35 µl rSAP (NEB) were added and sample was incubated for at least 8 h at 37 °C ...
-
bioRxiv - Molecular Biology 2024Quote: ... were annealed (81 µl of each Oligo 100 µM, 1x T4 ligase buffer; NEB) at 98 °C for 5 min ...
-
bioRxiv - Molecular Biology 2024Quote: ... and incubated in buffer IIa (0.2% SDS, 1x NEBuffer 3.1; NEB) for 10 min at 50 °C ...
-
bioRxiv - Molecular Biology 2024Quote: ... 850 U DpnII (NEB) and 35 µl rSAP (NEB ...
-
bioRxiv - Molecular Biology 2024Quote: ... The nuclei pellet was resuspended in buffer IV (160 µl annealed oligos, 1.2x T4 ligase buffer, 6,000 U T4 ligase, 1x protease inhibitors; NEB) to a final volume of 800 µl after washing it with 1x T4 ligase buffer (NEB ...
-
bioRxiv - Molecular Biology 2024Quote: ... For library preparation NEBnext Ultra II DNA Library Prep Kit (New England Biolabs) was used ...
-
bioRxiv - Molecular Biology 2024Quote: ... Buffer IIIb (3% Triton X-100, 1x T4 ligase buffer, 1x protease inhibitors; NEB, Sigma-Aldrich) was added to a final volume of 1 ml and sample was incubated for 15 min at 37 °C ...
-
bioRxiv - Molecular Biology 2024Quote: ... the nuclei pellet was washed with 1x NEBuffer 3.1 (NEB) and incubated in buffer IIa (0.2% SDS ...
-
bioRxiv - Genomics 2024Quote: ... 20 μl of Cutsmart buffer (NEB), 3 μl each of HinP1I (NEB R0124S) ...
-
bioRxiv - Genomics 2024Quote: ... and PCR amplified for 2 cycles using the NEBNext master mix (NEB M0544L) with Illumina Nextera primers and conditions ...
-
bioRxiv - Cancer Biology 2024Quote: ... and transformation (chemically competent NEB 5-alpha E. coli) were performed according to manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2024Quote: ... RNA was then extracted from washed beads using Trizol LS and treated with T4 Polynucleotide Kinase (New England Biolabs) to obtain 5’ phosphate ends for subsequent ligations and passed through NucAway columns (Ambion ...
-
bioRxiv - Microbiology 2024Quote: ... input and immunoprecipitated DNA were prepared into multiplexed libraries using NEBNext Ultra II DNA Library Prep Kit for Illumina (New England Biolabs, Ipswich, MA). For the RNA-seq library ...
-
bioRxiv - Cancer Biology 2024Quote: ... Shipston and was N-terminally attached to an RFP (BKCa-DECRFP) using KpnI and BamHI restriction sites after PCR amplification (NEB Q5 High-Fidelity DNA-Polymerase ...
-
bioRxiv - Microbiology 2024Quote: ... Then the enriched mRNA was fragmented into short fragments using fragmentation buffer and reversely transcribed into cDNA by using NEBNext Ultra RNA Library Prep Kit for Illumina (NEB #7530 ...
-
bioRxiv - Cancer Biology 2024Quote: ... the RNA was resuspended in an appropriate volume of RNase-free water supplemented with RNA inhibitors (New England Biolabs). RNA samples were stored at -80°C until further use ...
-
bioRxiv - Cancer Biology 2024Quote: ... the product was digested with the XmnI enzyme (NEB, R0194L) at 37°C for 3 hours ...
-
bioRxiv - Cancer Biology 2024Quote: ... restriction digest and subsequent ligation using T4 DNA ligase (New England Biolabs, #M0202). The RUNX1 HDR template was subcloned into plasmid pUC19 (Addgene ...
-
bioRxiv - Cancer Biology 2024Quote: ... Ligation of sequences was performed by PCR-based cloning via addition of specific restriction sites using the Phusion High-Fidelity PCR Master Mix (New England Biolabs, #F531L), restriction digest and subsequent ligation using T4 DNA ligase (New England Biolabs ...
-
bioRxiv - Biophysics 2024Quote: ... Elutions were pooled and loaded onto 3mL of Amylose Resin (New England Biolabs) that had been equilibrated with buffer E and incubated for 1 hr at 4°C ...
-
bioRxiv - Biophysics 2024Quote: ... SNAP-Cell 647-SiR (New England BioLabs) of 0.1 µM was administered into the culture medium ...
-
bioRxiv - Biophysics 2024Quote: ... Recombinant BimC plasmid was generated by integration of the BimC cDNA fragment into a modified Novagen pET-17b vector containing a 6xHis-tag and a GFP using Gibson Assembly (NEB). Plasmid region corresponds to the 71-1184 amino acids of BimC and the modified vector was amplified and assembled with KLD Enzyme Mix Reaction (NEB ...
-
bioRxiv - Biophysics 2024Quote: ... A 691 bp biotinylated DNA handle with a 29 nt 5′ overhang was amplified by Q5 DNA polymerase (NEB, Cat# M0491S) using a 5′ biotinylated forward primer and a reverse primer containing an abasic site 43 ...
-
bioRxiv - Biophysics 2024Quote: ... purified using a PCR clean up kit (NEB, Cat# T1030L) and eluted in water ...
-
bioRxiv - Biophysics 2024Quote: ... A 30 nt 3′ overhang was created by treating the purified PCR product with USER enzyme mix (NEB, Cat# M5505S) for 15 min at 37°C ...
-
bioRxiv - Biophysics 2024Quote: ... Transcription templates were prepared by PCR using primers complementary to the T7 promoter and terminator sequences (see Supplementary Table 1) or by digestion of the plasmid DNA with SphI (NEB, Cat# R3182S). mRNA fragments were prepared by in vitro transcription with T7 RNA polymerase using a ratio of 10:30 AU:GC NTPs ...
-
bioRxiv - Biophysics 2024Quote: ... and oligo-dT primers (New England Biolabs). Quantitative real-time PCR was performed in triplicates using iTaqUniversal SYBRGreen reagent (Bio-Rad ...
-
bioRxiv - Biophysics 2024Quote: 500 ng extracted total RNA was reverse transcribed into cDNA for each sample using Protoscript II reverse transcriptase (New England Biolabs) and oligo-dT primers (New England Biolabs) ...
-
bioRxiv - Cancer Biology 2024Quote: ... Assembly reaction was performed at 1:2 vector: insert ratio using the NEBuilder HiFi DNA Assembly Cloning Kit (New England Biolabs #E5520) according to the manufacturer’s protocol ...